scholarly journals Cytotoxic Activity of Crude Extracts as well as of Pure Components fromJatrophaSpecies, Plants Used Extensively in African Traditional Medicine

2011 ◽  
Vol 2011 ◽  
pp. 1-7 ◽  
Author(s):  
Olapeju O. Aiyelaagbe ◽  
Amao A. Hamid ◽  
Ernesto Fattorusso ◽  
Orazio Taglialatela-Scafati ◽  
Heinz C. Schröder ◽  
...  
Keyword(s):  
Cytotoxic Activity ◽  
Traditional Medicine ◽  
Neuronal Cell ◽  
Carcinoma Cells ◽  
Cervix Carcinoma ◽  
Highly Active ◽  
African Traditional Medicine ◽  
Growth Of Tumor ◽  
Mouse Lymphoma

Extracts fromJatropha curcas, a plant used in African traditional medicine for various diseases, were tested for cytotoxic activity. The root extracts strongly reduced cell growth of tumor cellsin vitro, a result consistent with the knowledge of the application of these plant extracts in traditional medicine, especially to cure/ameliorate cancer. A selection of pure diterpenoids existing in extracts fromJatrophaspecies and isolated fromJ. curcas, for example, curcusone C, curcusone D, multidione, 15-epi-4Z-jatrogrossidentadion, 4Z-jatrogrossidentadion, 4E-jatrogrossidentadion, 2-hydroxyisojatrogrossidion, and 2-epi-hydroxyisojatrogrossidion, were likewise tested, and they also showed strong cytotoxic activity. It turned out that these extracts are highly active against L5178y mouse lymphoma cells and HeLa human cervix carcinoma cells, while they cause none or only very low activity against neuronal cell, for example, PC12. These data underscore that extracts fromJ. curcasor pure secondary metabolites from the plant are promising candidates to be anticancer drug, combined with low neuroactive effects.

In vitro cytotoxic activity of Verbascum alceoides against cervix carcinoma cells

2020 ◽  
Vol 9 (1) ◽  
pp. 19
Author(s):  
Vajihe Akbari ◽  
Masoud Sadeghi Dinani ◽  
Samin Malakooti
Keyword(s):  
Cytotoxic Activity ◽  
Carcinoma Cells ◽  
Cervix Carcinoma

scholarly journals Cytotoxic Activity of Anticancer Drugs on Hepatocellular Carcinoma Cells in Hypoxic-Hyponutritional Culture

2014 ◽  
Vol 99 (6) ◽  
pp. 745-752 ◽  
Author(s):  
Qiang Li ◽  
Lin-Zhong Zhu ◽  
Ren-Jie Yang ◽  
Xu Zhu
Keyword(s):  
Hepatocellular Carcinoma ◽  
Cytotoxic Activity ◽  
Anticancer Drugs ◽  
Culture Medium ◽  
Carcinoma Cells ◽  
Diphenyltetrazolium Bromide ◽  
Pemetrexed Disodium ◽  
Higher Sensitivity ◽  
Arterial Chemoembolization

Abstract To investigate which anticancer drugs and combination of dual drugs could further promote the inhibition of cell growth in vitro against HCC cell line (HepG2) in the hypoxic and hyponutritional culture medium (HHCM) mimicked the different scenarios of transcatheter arterial chemoembolization (TACE). The cells of hepatocellular carcinoma (HCC) treated by TACE suffered various hypoxia and hyponutrition. The cells were treated for 2 hours, 4 hours, 6 hours, and 24 hours, respectively, using 10 drugs including epirubicin (EPI), cisplatin (DDP), mitomycin-C (MMC), oxaliplatin (OXA), hydroxycamptothecin (HCPT), 5-fluorouracil (5-FU), gemcitabine (GEM), docetaxel (DTX), thiotepa (TSPA), and pemetrexed disodium (PEM) in 4 concentrations of HHCM (5%, 10%, 25%, and 50%, respectively) mimicking the scenario of TACE and were assessed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The cells treated with combinations of dual drugs for 24 hours were also tested. The sensitive drugs with inhibition rates more than 30% were EPI, MMC, HCPT, OXA, and PEM in 4 types of HHCMs. The sensitivity of the cells to treatment with drugs for 24 hours was significantly higher than the sensitivity of the cells to treatment with drugs for 2 hours in 5%, 10%, and 25% HHCM. The sensitivity of the combination of dual drugs was no more than the sensitivity of the single drug with higher sensitivity in 4 concentrations of HHCM. EPI, MMC, HCPT, OXA, and PEM exhibited cytotoxic activity against HepG2 cells in various hypoxia and hyponutrition states. Prolonging the time of exposure could increase the sensitivity of drug, and the combination of dual drugs cannot enhance the cytotoxic effect.

Cytostatic Activity of Aeroplysinin-1 against Lymphoma and Epithelioma Cells

1989 ◽  
Vol 44 (7-8) ◽  
pp. 680-688 ◽  
Author(s):  
M. H. Kreuter ◽  
A. Bernd ◽  
H. Holzmann ◽  
W. Müller-Klieser ◽  
A. Maidhof ◽  
...  
Keyword(s):  
Test System ◽  
Marine Sponges ◽  
Carcinoma Cells ◽  
Nmri Mouse ◽  
Lymphoma Cells ◽  
Murine Spleen ◽  
Mouse Lymphoma Cells ◽  
Spleen Lymphocytes ◽  
Mouse Lymphoma

(±)-Aeroplysinin-1, an optically active 1.2-dihydroarene-1.2-diol. was isolated from the marine sponges Verongia aerophoba (+-isomer) and lanthella ardis (--isomer). For the experiments presented we used the +-isomer from Verongia aerophoba. Here we describe the hitherto unknown biological and pharmacological property of this compound to display pronounced anticancer activity against L5178y mouse lymphoma cells (ED50: 0.5 μm). Friend erythroleukemia cells (ED50: 0.7μm) , human mamma carcinoma cells (ED50: 0.3μm) and human colon carcinoma cells (ED50: 3.0 μm) in vitro. Furthermore, aeroplysinin caused a preferential inhibition of [3H]thymidine (dThd) incorporation rates in L5178y mouse lymphoma cells if compared with murine spleen lymphocytes in vitro. At concentrations between 1.1 and 28.5 μm, the [3H]dThd incorporation rates in L5178y cells were suppressed to 28% -0% but only to 78% -18% in murine spleen lymphocytes. The same differential effect in vitro was found with the following epithelial cells: 14.70 μm of the compound were required to inhibit normal human fibroblasts to 50% , but only 2.9 μm in the assays with human malign keratinocytes or malignant melanoma cells to observe the same inhibitory effect. Moreover, aeroplysinin-1 displayed antileukemic activity in vivo using the L5178y cell/NMRI mouse system; administered at a dose of 50 mg/kg for five consecutive days, the T/C (% ) value was determined to be 338. Preliminary toxicology studies revealed an acute LD50 of 202 mg/kg and a subacute LD50 of 150 mg/kg. Aeroplysinin-1 is neither a direct mutagen nor a premutagen in the umu/Salmonella typhimurium test system.

In vitro cytotoxic activity of nine plants used in Mayan traditional medicine

2009 ◽  
Vol 121 (3) ◽  
pp. 462-465 ◽  
Author(s):  
G. Mena-Rejon ◽  
E. Caamal-Fuentes ◽  
Z. Cantillo-Ciau ◽  
R. Cedillo-Rivera ◽  
J. Flores-Guido ◽  
...  
Keyword(s):  
Cytotoxic Activity ◽  
Traditional Medicine

scholarly journals Double-Edged Lipid Nanoparticles Combining Liposome-Bound TRAIL and Encapsulated Doxorubicin Showing an Extraordinary Synergistic Pro-Apoptotic Potential

Cancers ◽  
2019 ◽  
Vol 11 (12) ◽  
pp. 1948 ◽  
Author(s):  
Diego De Miguel ◽  
Ana Gallego-Lleyda ◽  
Miguel Martinez-Ara ◽  
Javier Plou ◽  
Alberto Anel ◽  
...  
Keyword(s):  
Cytotoxic Activity ◽  
Molecular Mechanisms ◽  
Xenograft Model ◽  
Carcinoma Cells ◽  
Tumor Xenograft ◽  
Cytotoxic Potential ◽  
Primary Tumors ◽  
Soluble Trail

Although TRAIL (TNF-related apoptosis-inducing ligand, also known as Apo2L) was described as capable of inducing apoptosis in transformed cells while sparing normal cells, limited results obtained in clinical trials has limited its use as an anti-tumor agent. Consequently, novel TRAIL formulations with enhanced bioactivity are necessary for overcoming resistance to conventional soluble TRAIL (sTRAIL) exhibited by many primary tumors. Our group has generated artificial liposomes with sTRAIL anchored on their surface (large unilamellar vesicle (LUV)-TRAIL), which have shown a greater cytotoxic activity both in vitro and in vivo when compared to sTRAIL against distinct hematologic and epithelial carcinoma cells. In this study, we have improved LUV-TRAIL by loading doxorubicin (DOX) in its liposomal lumen (LUVDOX-TRAIL) in order to improve their cytotoxic potential. LUVDOX-TRAIL killed not only to a higher extent, but also with a much faster kinetic than LUV-TRAIL. In addition, the concerted action of the liposomal DOX and TRAIL was specific of the liposomal DOX and was not observed when with soluble DOX. The cytotoxicity induced by LUVDOX-TRAIL was proven to rely on two processes due to different molecular mechanisms: a dynamin-mediated internalization of the doxorubicin-loaded particle, and the strong activation of caspase-8 exerted by the liposomal TRAIL. Finally, greater cytotoxic activity of LUVDOX-TRAIL was also observed in vivo in a tumor xenograft model. Therefore, we developed a novel double-edged nanoparticle combining the cytotoxic potential of DOX and TRAIL, showing an exceptional and remarkable synergistic effect between both agents.

scholarly journals Three New Iridoid Derivatives Have Been Isolated from the Stems of Neonauclea reticulata (Havil.) Merr. with Cytotoxic Activity on Hepatocellular Carcinoma Cells

Molecules ◽  
2018 ◽  
Vol 23 (9) ◽  
pp. 2297 ◽  
Author(s):  
Fang-Pin Chang ◽  
Wei Chao ◽  
Sheng-Yang Wang ◽  
Hui-Chi Huang ◽  
Ping-Jyun Sung ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Abscisic Acid ◽  
Cytotoxic Activity ◽  
Spectroscopic Data ◽  
Carcinoma Cells ◽  
Coumaric Acid ◽  
Cytotoxic Assay ◽  
Hep3b Cells ◽  
Hcc Cells

Three new iridoids, namely neonanin A (1), neonanin B (2) and neoretinin A (3), as well as twelve known compounds, 6-hydroxy-7-methyl-1-oxo-4-carbomethoxyoctahydrocyclopenta[c]pyran (4), 4-epi-alyxialactone (5), loganetin (6), loganin (7), phenylcoumaran-α′-aldehyde (8), cleomiscosin A (9), ficusal (10), balanophonin (11), vanillic acid (12), p-coumaric acid (13), cis,trans-abscisic acid (14), and trans,trans-abscisic acid (15) were isolated from the stems of Neonauclea reticulata (Havil.) Merr. These new structures were determined by the detailed analysis of spectroscopic data and comparison with the data of known analogues. Compounds 1–13 were evaluated using an in-vitro MTT cytotoxic assay for hepatocellular carcinoma (HCC) cells, and the preliminary results showed that ficusal (10), balanophonin (11), and p-coumaric acid (13) exhibited moderate cytotoxic activity, with EC50 values of 85.36 ± 4.36, 92.63 ± 1.41, and 29.18 ± 3.48 µg/mL against Hep3B cells, respectively.

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