scholarly journals M. tuberculosisin Lymph Node Biopsy Paraffin-Embedded Sections

2011 ◽  
Vol 2011 ◽  
pp. 1-5 ◽  
Author(s):  
Abdurehman Eshete ◽  
Ahmed Zeyinudin ◽  
Solomon Ali ◽  
Solomon Abera ◽  
Mona Mohammed
Keyword(s):  
Lymph Node ◽  
Histopathological Examination ◽  
Extrapulmonary Tuberculosis ◽  
Staining Technique ◽  
Cross Sectional Study ◽  
Lymph Node Biopsy ◽  
Medical Laboratory ◽  
Cross Sectional ◽  
Node Biopsy ◽  
Nodal Tissue

Background. Tuberculosis lymphadenitis is one of the most common forms of all extrapulmonary tuberculosis.Objective. To evaluate the magnitude ofM. tuberculosisfrom lymph node biopsy paraffin-embedded sections among suspected patients visiting the Jimma University Specialized Hospital.Method. A cross-sectional study design of histological examination among lymph node biopsy paraffin-embedded sections by Ziehl-Neelsen and hematoxylin/eosin staining technique was conducted from December, 2009, to October, 2010, at the Department of Medical Laboratory Science and Pathology.Result. Histopathological examination of the specimens by hematoxylin and eosin staining technique revealed the presence of granulomas. But for the caseation and necrosis they were present in 85% cases of nodal tissue biopsies. From those, 56.7% were from females. The presence of acid-fast bacilli was microscopically confirmed by ZN staining in 37 (61.7%) of the nodal tissue biopsies.Conclusion and Recommendation. Tuberculosis lymphadenitis is significantly more common in females. Hence, attention should be given for control and prevention of extrapulmonary tuberculosis.

scholarly journals Evaluation of the Yield of Histopathology in the Diagnosis of Lymph Node Tuberculosis in Morocco, 2017: Cross-Sectional Study (Preprint)

2019 ◽  
Author(s):  
Kenza Bennani ◽  
Asmae Khattabi ◽  
Mohammed Akrim ◽  
Mohamed Mahtar ◽  
Najib Benmansour ◽  
...  
Keyword(s):  
Lymph Node ◽  
Predictive Value ◽  
Clinical Evidence ◽  
Histopathological Examination ◽  
Extrapulmonary Tuberculosis ◽  
Cross Sectional Study ◽  
Sectional Study ◽  
Cross Sectional ◽  
Caseous Necrosis ◽  
Lymph Node Tuberculosis

BACKGROUND The frequency of occurrence of extrapulmonary tuberculosis (EPTB) has been increasing globally over the last two decades. In Morocco, EPTB cases account for 46% of the patients reported with a new episode of tuberculosis (TB). Lymph node TB (LNTB) is the most common form of EPTB. In line with the guidelines of the National TB Program, the diagnosis is mainly based on clinical evidence, including histopathology. OBJECTIVE This study aimed to evaluate the yield of histopathology testing in the diagnosis of LNTB. METHODS This cross-sectional, prospective study was conducted among patients with cervical lymph node who were enrolled in the study from November 2016 to May 2017 in three regions of Morocco. We compared the outcomes of histopathological testing with those of bacteriology. Sensitivity (Se), specificity (Sp), positive predictive value (PPV), and negative predictive value (NPV) of histopathology testing were calculated. Culture and Xpert tests were used as the gold standard Laboratoty Testing. RESULTS A total of 262 patients were enrolled in this study. The Se, Sp, PPV, and NPV of histopathology testing were 95.6% (129/135), 64.6% (82/127), 74.1% (129/174), and 93.2% (82/88), respectively, in the presence of granuloma with or without caseous necrosis and were 84.4% (114/135), 74.8% (95/127), 78.1% (114/146), and 81.9% (95/116), respectively, in the presence of granuloma with caseous necrosis. The granuloma with caseous necrosis was associated with increased PPV and Sp of histopathology testing (P<.05). CONCLUSIONS The presence of the granuloma with caseous necrosis in the histopathological examination had significantly improved the yield of histopathology testing for the diagnosis of LNTB. The findings recommend to maintain histopathology testing in establishing the LNTB diagnosis and to explore other techniques to improve it.

scholarly journals Evaluation of the Yield of Histopathology in the Diagnosis of Lymph Node Tuberculosis in Morocco, 2017: Cross-Sectional Study

10.2196/14252 ◽  
2019 ◽  
Vol 5 (4) ◽  
pp. e14252 ◽  
Author(s):  
Kenza Bennani ◽  
Asmae Khattabi ◽  
Mohammed Akrim ◽  
Mohamed Mahtar ◽  
Najib Benmansour ◽  
...  
Keyword(s):  
Lymph Node ◽  
Predictive Value ◽  
Clinical Evidence ◽  
Histopathological Examination ◽  
Extrapulmonary Tuberculosis ◽  
Cross Sectional Study ◽  
Sectional Study ◽  
Cross Sectional ◽  
Caseous Necrosis ◽  
Lymph Node Tuberculosis

Background The frequency of occurrence of extrapulmonary tuberculosis (EPTB) has been increasing globally over the last two decades. In Morocco, EPTB cases account for 46% of the patients reported with a new episode of tuberculosis (TB). Lymph node TB (LNTB) is the most common form of EPTB. In line with the guidelines of the National TB Program, the diagnosis is mainly based on clinical evidence, including histopathology. Objective This study aimed to evaluate the yield of histopathology testing in the diagnosis of LNTB. Methods This cross-sectional, prospective study was conducted among patients with cervical lymph node who were enrolled in the study from November 2016 to May 2017 in three regions of Morocco. We compared the outcomes of histopathological testing with those of bacteriology. Sensitivity (Se), specificity (Sp), positive predictive value (PPV), and negative predictive value (NPV) of histopathology testing were calculated. Culture and Xpert tests were used as the gold standard Laboratoty Testing. Results A total of 262 patients were enrolled in this study. The Se, Sp, PPV, and NPV of histopathology testing were 95.6% (129/135), 64.6% (82/127), 74.1% (129/174), and 93.2% (82/88), respectively, in the presence of granuloma with or without caseous necrosis and were 84.4% (114/135), 74.8% (95/127), 78.1% (114/146), and 81.9% (95/116), respectively, in the presence of granuloma with caseous necrosis. The granuloma with caseous necrosis was associated with increased PPV and Sp of histopathology testing (P<.05). Conclusions The presence of the granuloma with caseous necrosis in the histopathological examination had significantly improved the yield of histopathology testing for the diagnosis of LNTB. The findings recommend to maintain histopathology testing in establishing the LNTB diagnosis and to explore other techniques to improve it.

Staging N0 oral cancer: lymphoscintigraphy and conventional imaging

2005 ◽  
Vol 46 (5) ◽  
pp. 492-496 ◽  
Author(s):  
J. B. Thomsen ◽  
J. A. Sørensen ◽  
P. Grupe ◽  
J. Karstoft ◽  
A. Krogdahl
Keyword(s):  
Lymph Node ◽  
Sentinel Lymph Node ◽  
Sentinel Lymph Node Biopsy ◽  
Oral Cavity ◽  
Doppler Ultrasonography ◽  
Histopathological Examination ◽  
Lymph Node Biopsy ◽  
Predictive Values ◽  
Node Biopsy ◽  
Neck Disease

Purpose: To compare sentinel lymph node biopsy, magnetic resonance imaging (MRI), Doppler ultrasonography, and palpation as staging tools in patients with T1/T2 N0 cancer of the oral cavity. Material and Methods: Forty consecutive patients were enrolled (17 F and 23 M, aged 32–90 years), 24 T1 and 16 T2 cN0 squamous cell carcinoma of the oral cavity. Palpation was carried out by two observers prior to inclusion. MRI, gray-scale and Doppler ultrasonography were performed. Lymphoscintigraphies were done after peritumoral injections of 99mTc labelled rheniumsulphide nanocolloid, followed by sentinel lymph node biopsy guided by a gamma probe and Patent Blue. Palpation, Doppler ultrasonography, MRI, and sentinel lymph node biopsy were compared to a combination of histopathology and follow-up. Diagnostic testing was performed using the x2 test. Results: Histopathological examination revealed metastatic spread to the neck in 14 of 40 patients. One patient had bilateral neck disease. Sentinel lymph node biopsy and ultrasonography were performed in 80 neck sides of 40 patients and MRI in 70 neck sides (5 patients were claustrophobic). SN revealed suspicious lymph nodes in 12 necks, ultrasonography in 23 necks, and MRI in 9 necks. The positive predictive value of sentinel lymph node biopsy was 100%, ultrasonography 57%, and MRI 56%. The respective negative predictive values were 96%, 96%, and 85%. The sensitivity of sentinel lymph node biopsy 80% was comparable to ultrasonography 87%, but the sensitivity of MRI 36% was low. The specificities were 100%, 85%, and 93%, respectively. By combined sentinel lymph node biopsy and ultrasonography the overall sensitivity could have been 100%. Conclusion: Sentinel lymph node biopsy improved staging of patients with small N0 oral cancers. Combined sentinel lymph node biopsy and Doppler ultrasonography may further improve staging. MRI and simple palpation results were poor.

scholarly journals Influence of mammography screening in the treatment of women diagnosed with breast cancer

Mastology ◽  
2020 ◽  
Vol 30 (Suppl 1) ◽  
Author(s):  
Luiza da Rosa Ramos ◽  
Laura Becker Carminatti ◽  
Laura Gazola Ugioni
Keyword(s):  
Breast Cancer ◽  
Lymph Node ◽  
Mammography Screening ◽  
Secondary Data ◽  
Conservative Surgery ◽  
Lymph Node Involvement ◽  
Cross Sectional Study ◽  
Lymph Node Biopsy ◽  
Cross Sectional ◽  
Previous Diagnosis

Introduction: Apart from non-melanoma skin tumors, breast cancer is the most prevalent neoplasm among women in Brazil and worldwide. Breast cancer is a very heterogeneous disease, a fact attributed to the plasticity of its cells. Therefore, the stratification of tumors is critical to achieving better clinical outcomes. Breast cancer screening often allows diagnosing the disease in its earlier stages, manifested as smaller tumors, and still without lymph node involvement. The multiple prognostic factors that must be taken into account when considering the eligibility for treatment, such as age, reproductive status, type of cancer, and severity of the disease, make it impossible to establish clear approach guidelines for the disease, given the many different clinical situations. Objectives: To evaluate the influence of mammography screening in the treatment of women with a previous diagnosis of breast cancer. Methodology: This is an observational, descriptive, cross-sectional study conducted with primary and secondary data and a quantitative approach. The research was carried out in a high-complexity hospital in the far-south of Santa Catarina, Southern Brazil, and assessed patients with a previous diagnosis of breast cancer, from 2012 to 2017, who were on oncological outpatient follow-up. Results: Among the 99 patients analyzed, 58.6% undergo the examination annually, and 49.5% were diagnosed less than 12 months after the last mammography. Stage I diseases were more frequent, corroborating the finding that 74.7% of patients were submitted to conservative surgeries and 68.7% to sentinel lymph node biopsy, instead of more extensive procedures. With respect to the treatment of choice, patients with annual or biennial mammography frequency had surgical and chemotherapeutic outcomes similar to those of women who had no set frequency or who had never had a screening, that is, 72.4% of patients with annual frequency and 100% of patients with biennial frequency were submitted to conservative surgery, as occurred with 85% of patients with no set frequency and 66% of those who had never had the test. Conclusion: Patients who had annual mammographies and those diagnosed less than 12 months after the last mammography presented smaller tumors at diagnosis, but these characteristics did not influence the type of treatment chosen.

scholarly journals Lymphadenopathy in a TB-Endemic Region: Employing Focused Techniques in a Rapid Access Lymph Node Biopsy Clinic

Blood ◽  
2018 ◽  
Vol 132 (Supplement 1) ◽  
pp. 5318-5318
Author(s):  
Katherine Rae Antel ◽  
Estelle Verburgh ◽  
Vernon J. Louw ◽  
Jenna Oosthuizen ◽  
Francois Malherbe ◽  
...  
Keyword(s):  
Lymph Node ◽  
Core Needle Biopsy ◽  
Needle Biopsy ◽  
Extrapulmonary Tuberculosis ◽  
Lymph Node Biopsy ◽  
Endemic Region ◽  
Core Needle ◽  
Rapid Access ◽  
Tb Treatment ◽  
Node Biopsy

Abstract Introduction Lymphoma and extrapulmonary tuberculosis (EPTB) are common in HIV and may cause peripheral lymphadenopathy. EPTB can be difficult to diagnose and consequently, patients with lymphadenopathy in TB-endemic regions are frequently placed on empiric TB treatment. This may result in a missed or delayed diagnosis of lymphoma. In the local setting, in Cape Town (South Africa), primary level doctors have difficulty accessing lymph node excision biopsies which rely on an over-burdened surgical service. Core-needle biopsy is not currently routinely performed for peripheral lymphadenopathy in the investigation of lymphadenopathy. Methods A prospective study of patients with peripheral lymphadenopathy >2cm was performed. A rapid access lymph node biopsy clinic examined the performance of three methods of investigation on a lymph node: the fine-needle aspirate for cytology (FNAC), the Xpert MTB/Rif Ultra (on both aspirate and tissue specimens), and the ultrasound-guided core-needle biopsy. In all patients a final histological diagnosis was sought, including referral for excision biopsy if core-needle histology was inconclusive. If >0.5ml of pus was aspirated this was tested with the Xpert MTB/Rif and a biopsy was not performed. Results Sixty-two patients including 48% HIV+ have undergone investigation. The final diagnosis is known for 56 patients: lymphoma in 34%, TB in 30%, cancer in 12%. Ultra had a 64% sensitivity on the aspirate (95% CI 31-89%), and 73% on tissue (95% CI 39-94). In both cases specificity was 100%. FNAC was diagnostic in 0% of lymphoma cases (reported atypical in 24%); it was diagnostic for TB (+AFBs) in 27% with TB; and diagnostic in 36% of disseminated cancer cases. The core biopsy was able to make a diagnosis in 83% of cases (including sub-typing in 89% of lymphoma cases). Conclusion The Ultra shows promising sensitivity and should routinely be performed for both HIV+ and -patients at the first visit in the investigation of unexplained lymphadenopathy in a TB-endemic region. If the Ultra is negative, a core-needle biopsy is the most appropriate next investigation. In a TB-endemic region, FNAC should be reserved for patients >50 years who are HIV negative. There is no role for empiric TB treatment for patients with peripheral lymphadenopathy in the era of new TB diagnostic tests, and the application of these investigations with early biopsy for patients with negative TB tests will result in earlier detection of lymphoma. Disclosures No relevant conflicts of interest to declare.

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