scholarly journals The Chick Chorioallantoic Membrane: A Model of Molecular, Structural, and Functional Adaptation to Transepithelial Ion Transport and Barrier Function during Embryonic Development

2010 ◽  
Vol 2010 ◽  
pp. 1-12 ◽  
Author(s):  
Maria Gabriella Gabrielli ◽  
Daniela Accili
Keyword(s):  
Calcium Transport ◽  
Chorioallantoic Membrane ◽  
Allantoic Fluid ◽  
Model System ◽  
Functional Adaptation ◽  
Acid Base ◽  
Functional Potential ◽  
Chick Chorioallantoic Membrane ◽  
Wide Range ◽  
Respiratory Gases

The chick chorioallantoic membrane is a very simple extraembryonic membrane which serves multiple functions during embryo development; it is the site of exchange of respiratory gases, calcium transport from the eggshell, acid-base homeostasis in the embryo, and ion andH2O reabsorption from the allantoic fluid. All these functions are accomplished by its epithelia, the chorionic and the allantoic epithelium, by differentiation of a wide range of structural and molecular peculiarities which make them highly specialized, ion transporting epithelia. Studying the different aspects of such a developmental strategy emphasizes the functional potential of the epithelium and offers an excellent model system to gain insights into questions partly still unresolved.

scholarly journals Transcriptome-based insights into the calcium transport mechanism of chick chorioallantoic membrane

2022 ◽  
Vol 11 (2) ◽  
pp. 383-392
Author(s):  
Qun Huang ◽  
Ran Yang ◽  
Qia Wang ◽  
Hui Teng ◽  
Hongbo Song ◽  
...  
Keyword(s):  
Calcium Transport ◽  
Transport Mechanism ◽  
Chorioallantoic Membrane ◽  
Chick Chorioallantoic Membrane

Transepithelial calcium transport in the chick chorioallantoic membrane. II. Compartmentalization of calcium during uptake

1993 ◽  
Vol 105 (2) ◽  
pp. 381-388 ◽  
Author(s):  
R.E. Akins ◽  
R.S. Tuan
Keyword(s):  
Calcium Transport ◽  
Chorioallantoic Membrane ◽  
Cytosolic Calcium ◽  
Calcium Flux ◽  
Free Calcium ◽  
Extracellular Medium ◽  
Calcium Accumulation ◽  
Chick Chorioallantoic Membrane ◽  
Cellular Compartments ◽  
Endosomal Vesicles

Calcium transport from the eggshell to the developing chick embryo is carried out by the ectoderm cells of the chick chorioallantoic membrane. Primary cells isolated from chick chorioallantoic membrane ectoderm were used to analyze the subcellular distribution of 45Ca2+ accumulated from the extracellular medium. We present evidence suggesting that calcium may be sequestered into endosome-like vesicles during the initial phase of uptake. A combination of techniques were utilized to monitor calcium fluxes and calcium compartmentalization in the cultured chorioallantoic membrane cells: (1) fura-2 fluorescence was used to indicate cytosolic free calcium concentrations, (2) 45Ca2+ tracer was used to follow calcium accumulation in all cellular compartments, and (3) digitonin was used to differentially permeabilize subcellular membranes in order to localize 45Ca2+ by following tracer release profiles. Differences between cytosolic calcium flux and whole cell calcium accumulation suggested that the pathway of calcium uptake from the medium involves sequestration into an internal compartment separate from the cytosol. Kinetic analysis of the digitonin-mediated release of specific subcellular markers (lactate dehydrogenase, NAD-dependent isocitrate dehydrogenase, [3H]inulin, and [3H]-2-deoxyglucose) and preloaded 45Ca2+ indicated that calcium was localized in a compartment similar to endosomal vesicles. Our results are consistent with a transcytotic mechanism for chorioallantoic membrane calcium transport.

scholarly journals Calcium-binding protein of the chick chorioallantoic membrane. I. Immunohistochemical localization

1978 ◽  
Vol 77 (3) ◽  
pp. 743-751 ◽  
Author(s):  
RS Tuan ◽  
WA Scott ◽  
ZA Cohn
Keyword(s):  
Calcium Binding ◽  
Calcium Transport ◽  
External Surface ◽  
Binding Protein ◽  
Chorioallantoic Membrane ◽  
Immunohistochemical Localization ◽  
Calcium Binding Protein ◽  
Transport Function ◽  
Chick Chorioallantoic Membrane

The preparation of a specific antiserum (anti-CaBP) against the calcium-binding protein (CaBP) of the chorioallantoic membrane (CAM) is described. The anti-CaBP appeared to be specific for the CaBP by immunodiffusion and immunoelectrophoresis. Application of the anti-CaBP in immunofluorescence histochemistry revealed that the CaBP is present in the CAM only at developmental ages corresponding with the expression of the calcium transport function of the membrane. Furthermore, the CaBP is localized to the ectoderm of the CAM, appears to be exposed to the entire external surface of the ectoderm, and can be shown to be associated with cells enzymatically dissociated from the CAM. These results are consistent with a functional role of the CaBP in the CAM calcium transport process.

scholarly journals Angiogenic and invasive properties of neurofibroma Schwann cells.

1990 ◽  
Vol 111 (2) ◽  
pp. 645-653 ◽  
Author(s):  
S Sheela ◽  
V M Riccardi ◽  
N Ratner
Keyword(s):  
Extracellular Matrix ◽  
Schwann Cells ◽  
Chorioallantoic Membrane ◽  
Model System ◽  
Basement Membranes ◽  
Benign Tumors ◽  
Epigenetic Changes ◽  
Normal Cells ◽  
Chick Chorioallantoic Membrane ◽  
Von Recklinghausen

Neurofibromas are benign tumors from patients with von Recklinghausen Neurofibromatosis (NF1) that are comprised primarily of Schwann cells. These Schwann cells are found both in association with axons and in the extracellular matrix that is prevalent in neurofibromas, and in which fibroblasts are also abundant. An unresolved question has been whether cells in neurofibromas are normal cells or are intrinsically abnormal. We have tested the hypothesis that cells in neurofibromas are abnormal and have shown that neurofibroma Schwann cells, unlike normal Schwann cells, promote angiogenesis in the chick chorioallantoic membrane model system, and invade basement membranes in this system. In contrast, neurofibroma fibroblasts neither promote angiogenic reactions nor invade basement membranes. When injected into nude mice, neurofibroma Schwann cells do not form progressive tumors. These results suggest that NF1 Schwann cells differ from normal Schwann cells, that they are preneoplastic, and that genetic and/or epigenetic changes in Schwann cells may be required for development of peripheral nerve tumors in NF1.

scholarly journals Fetal Mouse Skin Heals Scarlessly in a Chick Chorioallantoic Membrane Model System

2012 ◽  
Vol 69 (1) ◽  
pp. 85-90 ◽  
Author(s):  
Antoine L. Carre ◽  
Barrett J. Larson ◽  
Joseph A. Knowles ◽  
Kenichiro Kawai ◽  
Michael T. Longaker ◽  
...  
Keyword(s):  
Chorioallantoic Membrane ◽  
Mouse Skin ◽  
Model System ◽  
Membrane Model ◽  
Fetal Mouse ◽  
Chick Chorioallantoic Membrane
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