scholarly journals Comparative Study on Two Commercial Strains ofSaccharomyces cerevisiaefor Optimum Ethanol Production on Industrial Scale

2010 ◽  
Vol 2010 ◽  
pp. 1-5 ◽  
Author(s):  
K. Mukhtar ◽  
M. Asgher ◽  
S. Afghan ◽  
K. Hussain ◽  
S. Zia-ul-Hussnain
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Yeast Cell ◽  
Ethanol Production ◽  
Yeast Strain ◽  
Fermentation Process ◽  
Cell Concentration ◽  
Inoculum Size ◽  
Summer Season ◽  
Maximum Conversion ◽  
Fermentation Parameters

Two commercial strains of Saccharomyces cerevisiae, Saf-Instant (Baker's yeast) and Ethanol red (Mutant) were compared for ethanol production during hot summer season, using molasses diluted up to 6- Brix containing 4%-5% sugars. The yeasts were then propagated in fermentation vessels to study the effects of yeast cell count and varying concentrations of Urea, DAP, inoculum size and Lactrol (Antibiotic). Continuous circulation of mash was maintained for 24 hours and after this fermenter was allowed to stay for a period of 16 hours to give time for maximum conversion of sugars into ethanol. Saccharomyces cerevisiae strain (Saf-instant) with cell concentration of 400 millions/mL at molasses sugar level of 13%–15% (pH , Temp. ), inoculum size of 25% (v/v), urea concentration, 150 ppm, DAP, 53.4 ppm and Lactrol,150 ppm supported maximum ethanol production (8.8%) with  L ethanol per tone molasses (96.5% yield), and had significantly lower concentrations of byproducts. By selecting higher ethanol yielding yeast strain and optimizing the fermentation parameters both yield and economics of the fermentation process can be improved.

Optimization of temperature, sugar concentration, and inoculum size to maximize ethanol production without significant decrease in yeast cell viability

2009 ◽  
Vol 83 (4) ◽  
pp. 627-637 ◽  
Author(s):  
Cecilia Laluce ◽  
João Olimpio Tognolli ◽  
Karen Fernanda de Oliveira ◽  
Crisla Serra Souza ◽  
Meline Rezende Morais
Keyword(s):  
Cell Viability ◽  
Yeast Cell ◽  
Ethanol Production ◽  
Inoculum Size ◽  
Sugar Concentration

Ethanol Production from Different Substrates by a Flocculent Saccharomyces cerevisiae Strain

2009 ◽  
Vol 7 (1) ◽  
Author(s):  
José Duarte ◽  
Vera Lourenço ◽  
Belina Ribeiro ◽  
Maria Céu Saagua ◽  
Joana Pereira ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Ethanol Production ◽  
Yeast Strain ◽  
Fossil Fuels ◽  
Ethanol Yield ◽  
Corn Fiber ◽  
Production Costs ◽  
Raw Material ◽  
Continuous Reactor ◽  
Different Strains

During the last years there has been an increasing interest in using ethanol as a substitute for fossil fuels. The bioethanol used today is mainly produced from sugar cane and cereals, but reducing the production costs of ethanol is still crucial for a viable economic process. Cellulose from vegetable biomass will be the next cheap raw material for second generation fuel ethanol production and agricultural by-products with a low commercial value, as corn stover, corn fiber and cane bagasses would become an attractive feedstock for bioethanol production.In this study, different strains of Saccharomyces cerevisiae have been screened for the ability of bioethanol production. Yeasts were grown in a synthetic liquid medium containing sucrose in batch regime and the growth rates, ethanol and biomass productions were determined as well as their growth ability in cane molasses.The results indicate that a flocculent yeast, isolated in our lab and designated by strain F, was the most promising yeast strain among those tested for continuous ethanol production. This strain was isolated from corn hydrolysates, obtained from a Portuguese distillery facility (DVT, Torres Novas, Portugal) showing highest growth rate (0.49h-1), highest ethanol yield (0.35g/g) and high flocculation capacity.The study on ethanol production in continuous reactor process with the selected yeast strain (strain F) was made on sucrose and cane molasses at different dilution rates (0.05-0.42 h-1). A steady flocculating yeast fluidized bed reactor system was established allowing the functioning of the reactor for 1000 h. Data shows that when the dilution rate rose to 0.42h-1, the highest productivity (20g/Lh) was obtained attaining an ethanol concentration in the reactor of 47g/L for sucrose and molasses media.

scholarly journals Production of bioethanol from amla (Emblica officinalis Gaertn.)

2021 ◽  
pp. 66-69
Author(s):  
Minal Deolekar ◽  
Trupti Shende
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Ethanol Production ◽  
Fermentation Process ◽  
Emblica Officinalis ◽  
Iodometric Titration ◽  
Titration Method ◽  
Herbal Products ◽  
Yeast Peptone Dextrose ◽  
Increasing Demand ◽  
Ethanol Ethanol

On account of the increasing demand for valuable herbal products, an attempt was made to produce a functional fermented Ethanol from Amla. This study investigates the potential of ethanol production from Amla (Emblica officinalis Gaertn). In the present study, Amla juice was extracted, filtered, fermented and it shows a suitable medium for the growth of Saccharomyces cerevisiae on yeast peptone dextrose medium for the production of ethanol. Ethanol was separated by fractional distillation and then estimated at 4, 6, 8 and 10 days of the fermentation process by iodometric method for 30oC. The ethanol percentage estimated by the iodometric titration method was high on the 10th day, and it was found to be 1.63 gm% compared to all days. So, the outcome of this study reveals that amla fruit can be used as a crucial constituent for the yield of ethanol with a higher commercial value.

Inactivation of maltose permease and maltase in sporulatingSaccharomyces cerevisiae

2000 ◽  
Vol 46 (4) ◽  
pp. 383-386 ◽  
Author(s):  
Julio C Ferreira ◽  
Anita D Panek ◽  
Pedro S de Araujo
Keyword(s):  
Gene Expression ◽  
Saccharomyces Cerevisiae ◽  
Yeast Strain ◽  
Cell Concentration ◽  
Alternative Mechanism ◽  
Sporulation Medium ◽  
Maltose Permease ◽  
Maltose Transport ◽  
Maltase Activity ◽  
Acetate Medium

Maltose transport and maltase activities were inactivated during sporulation of a MAL constitutive yeast strain harboring different MAL loci. Both activities were reduced to almost zero after 5 h of incubation in sporulation medium. The inactivation of maltase and maltose permease seems to be related to optimal sporulation conditions such as a suitable supply of oxygen and cell concentration in the sporulating cultures, and occurs in the fully derepressed conditions of incubation in the sporulation acetate medium. The inactivation of maltase and maltose permease under sporulation conditions in MAL constitutive strains suggests an alternative mechanism for the regulation of the MAL gene expression during the sporulation process.Key words: maltase activity, maltose permease activity, sporulation, Saccharomyces cerevisiae.

scholarly journals The evaluation of nanoparticles ZnO and TiO2 effects on Saccharomyces cerevisiae CNMN-Y-20 yeast strain

2016 ◽  
Vol 20 (1) ◽  
pp. 85-92 ◽  
Author(s):  
Agafia Usatîi ◽  
Natalia Chiseliţa ◽  
Nadejda Efremova
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cell Proliferation ◽  
Yeast Cell ◽  
Protein Content ◽  
Biomass Production ◽  
Yeast Strain ◽  
Cell Membranes ◽  
Carbohydrate Content

Abstract This paper investigates the action of nanoparticles ZnO (10 nm) and TiO2 (30 nm) on growth of Saccharomyces cerevisiae CNMN-Y-20 yeast. Nanoparticles in concentration of 0,5; 1,0 and 5,0 mg/L in YPD medium did not modify significantly cell proliferation, biomass production, the carbohydrate content and the content of β-glucans at Saccharomyces cerevisiae CNMN-Y-20. Nanoparticles ZnO and TiO2 contributed to the decrease in protein content, which demonstrated the appearance of the alterations of yeast cell membranes.

scholarly journals Production of bioethanol from amla (Emblica officinalis Gaertn.)

2021 ◽  
pp. 66-69
Author(s):  
Minal Deolekar ◽  
Trupti Shende
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Ethanol Production ◽  
Fermentation Process ◽  
Emblica Officinalis ◽  
Iodometric Titration ◽  
Titration Method ◽  
Herbal Products ◽  
Yeast Peptone Dextrose ◽  
Increasing Demand ◽  
Ethanol Ethanol

On account of the increasing demand for valuable herbal products, an attempt was made to produce a functional fermented Ethanol from Amla. This study investigates the potential of ethanol production from Amla (Emblica officinalis Gaertn). In the present study, Amla juice was extracted, filtered, fermented and it shows a suitable medium for the growth of Saccharomyces cerevisiae on yeast peptone dextrose medium for the production of ethanol. Ethanol was separated by fractional distillation and then estimated at 4, 6, 8 and 10 days of the fermentation process by iodometric method for 30oC. The ethanol percentage estimated by the iodometric titration method was high on the 10th day, and it was found to be 1.63 gm% compared to all days. So, the outcome of this study reveals that amla fruit can be used as a crucial constituent for the yield of ethanol with a higher commercial value.

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