scholarly journals Antioxidative Activity of Ferrocenes Bearing 2,6-Di-Tert-Butylphenol Moieties

2010 ◽  
Vol 2010 ◽  
pp. 1-6 ◽  
Author(s):  
E. R. Milaeva ◽  
S. I. Filimonova ◽  
N. N. Meleshonkova ◽  
L. G. Dubova ◽  
E. F. Shevtsova ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Rat Brain ◽  
Antioxidative Activity ◽  
Neuroprotective Effect ◽  
In Vitro Study ◽  
Brain Homogenate ◽  
Liver Mitochondria ◽  
Rat Liver Mitochondria ◽  
Calcium Dependent

The antioxidative activity of ferrocenes bearing either 2,6-di-tert-butylphenol or phenyl groups has been compared using DPPH (1,1-diphenyl-2-picrylhydrazyl) test and in the study of the in vitro impact on lipid peroxidation in rat brain homogenate and on some characteristics of rat liver mitochondria. The results of DPPH test at20∘C show that the activity depends strongly upon the presence of phenolic group but is improved by the influence of ferrocenyl fragment. The activity of N-(3,5-di-tert-butyl-4-hydroxyphenyl)iminomethylferrocene (1), for instance, was 88.4%, which was higher than the activity of a known antioxidant 2,6-di-tert-butyl-4-methylphenol (BHT) (48.5%), whereas the activity of N-phenyl-iminomethylferrocene2was almost negligible−2.9%. The data obtained demonstrate that the compounds with 2,6-di-tert-butylphenol moiety are significantly more active than the corresponding phenyl analogues in the in vitro study of lipid peroxidation in rat brain homogenate. Ferrocene1performs a promising behavior as an antioxidant and inhibits the calcium-dependent swelling of mitochondria. These results allow us to propose the potential cytoprotective (neuroprotective) effect of ditopic compounds containing antioxidant 2,6-di-tert-butylphenol group and redox active ferrocene fragment.

Magnetic nanostructured lipid carrier for dual triggered curcumin delivery: Preparation, characterization and toxicity evaluation on isolated rat liver mitochondria

2021 ◽  
pp. 088532822110346
Author(s):  
Mohammad Yoozbashi ◽  
Hamid Rashidzadeh ◽  
Mehraneh Kermanian ◽  
Somayeh Sadighian ◽  
Mir-Jamal Hosseini ◽  
...  
Keyword(s):  
In Vitro Study ◽  
Liver Mitochondria ◽  
Rat Liver Mitochondria ◽  
Nanostructured Lipid Carrier ◽  
Mitochondrial Toxicity ◽  
X Ray Diffraction ◽  
Temperature Dependent ◽  
Reducing Ability ◽  
Transmission Electron

In this research, magnetic nanostructured lipid carriers (Mag-NLCs) were synthesized for curcumin (CUR) delivery. NLCs are drug-delivery systems prepared by mixing solid and liquid (oil) lipids. For preparation of NLCs, cetylpalmitate was selected as solid lipid and fish oil as liquid lipid. CUR-Mag-NLCs were prepared using high-pressure homogenization technique and were characterized by methods including X-ray diffraction (XRD), transmission electron microscopy (TEM), vibrating sample magnetometer (VSM), and dynamic light scattering (DLS). The CUR-Mag-NLCs were developed as a particle with a size of 140 ± 3.6 nm, a polydispersity index of 0.196, and a zeta potential of −22.6 mV. VSM analysis showed that the CUR-Mag-NLCs have excellent magnetic properties. Release rate of the drug was higher at 42 °C than 37 °C, indicating that release of the synthesized nanoparticles is temperature-dependent. Evaluation of mitochondrial toxicity was done using the isolated rats liver mitochondria including glutathione (GSH), malondialdehyde (MDA), and the ferric- reducing ability of plasma (FRAP) assays to study biosafety of the CUR-Mag-NLCs. Results of In vitro study on the isolated mitochondria revealed that both CUR-Mag-NLCs and curcumin have no specific mitochondrial toxicity.

IN VITRO LIPID PEROXIDATION INHIBITORY AND ANTI-ARTHRITIC ACTIVITIES OF SOME INDIAN MEDICINAL PLANTS

INDIAN DRUGS ◽  
2012 ◽  
Vol 49 (06) ◽  
pp. 31-35
Author(s):  
A. A Rege ◽  
◽  
P. R. Juvekar ◽  
A. R. Juvekar
Keyword(s):  
Lipid Peroxidation ◽  
Liver Mitochondria ◽  
Tinospora Cordifolia ◽  
Ocimum Sanctum ◽  
Rat Liver Mitochondria ◽  
Total Phenolic ◽  
Lipid Peroxidation Inhibitory Activity ◽  
Gallic Acid Equivalent ◽  
Indian Medicinal Plants

Anti-lipid peroxidation effect of aqueous extracts of Ocimum sanctum, Tinospora cordifolia and Withania somnifera was evaluated against Fe2+-ascorbic acid-induced lipid peroxidation using rat liver mitochondria as model system, whereas, anti-arthritic activity was evaluated by proteinase inhibitory assay. O. sanctum showed potent anti-lipid peroxidation and anti-arthritic activities. T. cordifolia exhibited moderate anti-lipid peroxidation activity, but considerable anti-arthritic activity, whereas, W. somnifera revealed least lipid peroxidation inhibitory activity and considerable anti-arthritic activity. Besides, Folin-Ciocalteu reagent in terms of gallic acid equivalent achieved the total phenolic content and the trend was found to be O. sanctum > T. cordifolia > W. somnifera.

The effects of CeO2 nanoparticle (CeNPs) on oxidative stress biomarkers of rat liver mitochondria: In vitro study ‏

2020 ◽  
Vol 10 ◽  
Author(s):  
Mona Pourjafar ◽  
Sara Malih ◽  
Akram Ranjbar
Keyword(s):  
Oxidative Stress ◽  
Rat Liver ◽  
Antioxidant Properties ◽  
In Vitro Study ◽  
Liver Mitochondria ◽  
Rat Liver Mitochondria ◽  
Oxidative Stress Biomarkers ◽  
Beneficial Effects ◽  
Stress Biomarkers

: In recent years, the applications of nanoparticles have received a great attention due to their industrial and biomedical applications, while their beneficial effects suffer from controversial results at clinical stages. In the current study, cytotoxicity of cerium oxide (CeNP) nanoparticles (100 nm) were evaluated using mitochondria derived from wistar rat's liver. Isolated mitochondria from rat’s liver were divided into 7 groups including group 1 as control and group 2 to 7 as treatment group with different doses of CeNP (5, 10, 50, 100, 250 and 500mg/ml, respectively), for 24,48 and 72 hours. After exposure, oxidative stress biomarkers such as total ‎antioxidant capacity (TAC), lipid peroxidation (LPO), total thiol groups (TTG), catalase activity (CAT) and mitochondrial viability, were determined in isolated rat liver mitochondria. Results have shown that CeNPs increase TAC, TTG, CAT, LPO and viability of mitochondria in various exposure times and confirm antioxidant properties of CeNPs in mithocondria while mitochondria is a main source for the generation of reactive oxygen species (ROS).

scholarly journals Effect of diterpene alkaloids on lipid peroxidation in mitochondria

2021 ◽  
Vol 20 (2) ◽  
pp. e850
Author(s):  
Dilnoza Kh. Muratova ◽  
Nurali A. Ergashev ◽  
Jobir J. Sobirov ◽  
Utkir Kh. Kurbanov ◽  
Muzaffar I. Asrarov
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Protective Effect ◽  
Rat Liver ◽  
Liver Mitochondria ◽  
Rat Liver Mitochondria ◽  
Diterpenoid Alkaloids ◽  
Secondary Products ◽  
Diterpene Alkaloids

In this article, the antioxidant activity of some alkaloids lipid peroxidation (LPO) in rat liver mitochondria has been studied. It has been established that diterpenoid alkaloids: 1-О-benzoylnapelline, napelline and songorine have a protective effect on mitochondria, reducing the damaging effect of Fe2+/ascorbate and the release of malondialdehyde (MDA) into the secondary products of peroxidation. The effect of alkaloids napelline, 1-O-benzoylnapelline and songorine on the processes of MDA formation in rat liver mitochondria in vitro has been studied.Where in at 200 мM concentrations, 1-О-benzoylnapelline inhibited the formation of MDA by 95 %, and the alkaloids napelline and songorine at this concentration inhibited the formation of MDA by 54 and 44 %. From the data obtained, it can be shown that 1-О-benzoylnapelline strongly inhibits the formation of MDA compared to songorine and napelline.

scholarly journals Neuroprotective effect of Danshensu derivatives as anti-ischaemia agents on SH-SY5Y cells and rat brain

2013 ◽  
Vol 33 (4) ◽  
Author(s):  
Sunisa Seetapun ◽  
Jia Yaoling ◽  
Yang Wang ◽  
Yi Zhun Zhu
Keyword(s):  
Rat Brain ◽  
Infarct Volume ◽  
Neuroprotective Effect ◽  
In Vitro Study ◽  
Artery Occlusion ◽  
Neuroprotective Effects ◽  
Inhibit Lipid Peroxidation ◽  
Cysteine Derivative

Novel Danshensu derivatives (3–8) were designed and synthesized to improve bioactivity based on the strategy of ‘medicinal chemical hybridization’. Our previous studies indicated that these compounds exhibited noticeable cardioprotective activities. Here, we investigate whether these novel Danshensu derivatives exert neuroprotective activities. An in vitro study revealed that these compounds could increase cell viability and reduce LDH (lactate dehydrogenase) leakage. Moreover, Danshensu-cysteine derivative compounds 6 and 8 could significantly inhibit lipid peroxidation of cell membrane and regulate the expression of apoptosis-related protein (Bcl-2, Bax and caspase 3). An in vivo study demonstrated that treatment with compound 6 at 30 mg/kg markedly decreased the infarct volume of MCAO (middle cerebral artery occlusion) insulted rat brain. Furthermore, treatment with compound 6 showed the antioxidant capacity by increasing the activity of SOD (superoxide dismutase) and GPx (glutathione peroxidase) and decreasing the level of MDA (malondialdehyde) and the ROS (reactive oxygen species) production significantly. These results suggested that these novel conjugates exert significant neuroprotective effects as anti-ischaemia agents and those with high potential merit further investigation.

scholarly journals AN IN VITRO STUDY OF THE INTERACTION OF SEA-NINE® WITH RAT LIVER MITOCHONDRIA

2005 ◽  
Vol 24 (5) ◽  
pp. 1074 ◽  
Author(s):  
Marcantonio Bragadin ◽  
Bruno Pavoni ◽  
Guido Scutari ◽  
Sabrina Manente
Keyword(s):  
Rat Liver ◽  
In Vitro Study ◽  
Liver Mitochondria ◽  
Vitro Study ◽  
Rat Liver Mitochondria
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