scholarly journals Kinetic Spectrophotometric Determination of Certain Cephalosporins in Pharmaceutical Formulations

2009 ◽  
Vol 2009 ◽  
pp. 1-12 ◽  
Author(s):  
Mahmoud A. Omar ◽  
Osama H. Abdelmageed ◽  
Tamer Z. Attia
Keyword(s):  
Initial Rate ◽  
Pharmaceutical Formulations ◽  
Fixed Time ◽  
Rate Of Change ◽  
Ceftriaxone Sodium ◽  
Accuracy And Precision ◽  
Significant Difference ◽  
Cephalosporin Antibiotics ◽  
Kinetic Spectrophotometric

A simple, reliable, and sensitive kinetic spectrophotometric method was developed for determination of eight cephalosporin antibiotics, namely, Cefotaxime sodium, Cephapirin sodium, Cephradine dihydrate, Cephalexin monohydrate, Ceftazidime pentahydrate, Cefazoline sodium, Ceftriaxone sodium, and Cefuroxime sodium. The method depends on oxidation of each of studied drugs with alkaline potassium permanganate. The reaction is followed spectrophotometrically by measuring the rate of change of absorbance at 610 nm. The initial rate and fixed time (at 3 minutes) methods are utilized for construction of calibration graphs to determine the concentration of the studied drugs. The calibration graphs are linear in the concentration ranges 5–15 g  and 5–25 g  using the initial rate and fixed time methods, respectively. The results are validated statistically and checked through recovery studies. The method has been successfully applied for the determination of the studied cephalosporins in commercial dosage forms. Statistical comparisons of the results with the reference methods show the excellent agreement and indicate no significant difference in accuracy and precision.

scholarly journals New Kinetic Spectrophotometric Method for Determination of Fexofenadine Hydrochloride in Pharmaceutical Formulations

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Safwan Ashour ◽  
Mouhammed Khateeb
Keyword(s):  
Spectrophotometric Method ◽  
Initial Rate ◽  
Pharmaceutical Preparations ◽  
Pharmaceutical Formulations ◽  
Fixed Time ◽  
Official Method ◽  
Reaction Conditions ◽  
Accuracy And Precision ◽  
Kinetic Spectrophotometric

A simple and sensitive kinetic spectrophotometric method was developed for the determination of fexofenadine hydrochloride in bulk and pharmaceutical preparations. The method is based on a kinetic investigation of the oxidation reaction of fexofenadine using alkaline potassium permanganate as an oxidizing agent at room temperature. The reaction is followed spectrophotometrically by measuring the increase of absorbance owing to the formation of manganate ion at 610 nm. The initial rate and fixed time (at 15 min) methods are utilized for construction of calibration graphs. All the reaction conditions for the proposed method have been studied. The linearity range was found to be 2.5–50.0 μg mL−1 with detection limit of 0.055 μg mL−1 for both initial rate and fixed time methods. The proposed method was applied successfully for the determination of fexofenadine in pharmaceutical formulations; the percentage recoveries were 99.98–101.96%. The results obtained were compared statistically with those obtained by the official method and showed no significant differences regarding accuracy and precision.

scholarly journals Kinetic Spectrophotometric Method for the Estimation of Ofloxacin in Pharmaceutical Formulations

2017 ◽  
Vol 56 (4) ◽  
Author(s):  
Lalit Kishore ◽  
Ashok Kumar ◽  
Anroop Nair ◽  
Navpreet Kaur
Keyword(s):  
Spectrophotometric Method ◽  
Reference Method ◽  
Pharmaceutical Formulations ◽  
Fixed Time ◽  
Rate Of Change ◽  
Accuracy And Precision ◽  
Ich Guidelines ◽  
Significant Difference ◽  
Rate Method ◽  
Kinetic Spectrophotometric

The objective of the current study was to develop a direct, sensitive spectrophotometric method based on the oxidation of Ofloxacin using potassium permanganate in alkaline medium. The rate of change of absorbance was measured at 603 nm. The initial rate method and fixed time method (at 4 min) are utilised to construct calibration graphs for calculating the concentration of the drug. The results were validated through inter day and intraday precision assays according to the ICH guidelines and also through recovery studies. Statistical comparison of the proposed methods with that of reference method shows excellent agreement and indicates no significant difference in their accuracy and precision.

scholarly journals New Kinetic Spectrophotometric Method for Determination of Folic Acid in Pharmaceutical Formulations

2015 ◽  
Vol 50 ◽  
pp. 169-178
Author(s):  
Mouhammed Khateeb ◽  
Basheer Elias ◽  
Fatema Al Rahal
Keyword(s):  
Folic Acid ◽  
Spectrophotometric Method ◽  
Pharmaceutical Formulations ◽  
Fixed Time ◽  
Rate Data ◽  
Sulfuric Acid Medium ◽  
Significant Difference ◽  
Kinetic Spectrophotometric ◽  
Comparison Of The Results

A simple and sensitive kinetic spectrophotometric method has been developed for the determination of folic acid (FA) in bulk and pharmaceutical Formulations. The method is based on the oxidation of FA by Fe (III) in sulfuric acid medium. Fe (III) subsequently reduces to Fe (II) which is coupled with potassium ferricyanide to form Prussian blue. The reaction is followed spectrophotometrically by measuring the increase in absorbance at λmax 725 nm. The rate data and fixed time methods were adopted for constructing the calibration curves. The linearity range was found to be 1–20 μg mL-1 for each method. The correlation coefficient was 0.9978 and 0.9993, and LOD was found to be 0.91 and 0.09 μg mL-1 for rate data and fixed time methods, respectively. The proposed method has been successfully applied to the determination of FA in formulations with no interference from the excipients. Statical comparison of the results shows that there is no significant difference between the proposed and pharmacopoeial methods

scholarly journals A kinetic spectrophotometric method for the determination of lansoprazole in pharmaceutical formulations

2006 ◽  
Vol 71 (10) ◽  
pp. 1107-1120 ◽  
Author(s):  
Nafisur Rahman ◽  
Zehra Bano ◽  
Hejaz Azmi ◽  
Mohammad Kashif
Keyword(s):  
Spectrophotometric Method ◽  
Room Temperature ◽  
Pharmaceutical Formulations ◽  
Accuracy And Precision ◽  
Significant Difference ◽  
Kinetic Spectrophotometric ◽  
Comparison Of The Results ◽  
Calibration Equations ◽  
Method Show

Asimple kinetic spectrophotometric method has been developed for the determination of lansoprazole in pharmaceutical formulations. The method is based on the oxidation of the drug with alkaline potassium permanganate at room temperature. The reaction was followed spectrophotometrically by measuring the increase in the absorbance owing to the formation of MnO 42? at 610 nm (Method A) and the decrease in the absorbance at 530 nm due to the disapperance of MnO4? (Method B). Calibration procedures were adopted for the assay of the drug. The calibration curves were linear over the concentration ranges of 5-150 and 5-70?g ml-1, with the corresponding calibration Equations: rate = -3.915x10-6 + 5.271x10-5 c and ?A = 1.04x 10-3 + 1.78x10-3 c for methods A, and B, respectively. A statistical comparison of the results of the proposed procedures with those of the reference spectrophotometric method show excellent agreement and indicated no significant difference between the compared methods in terms of accuracy and precision. Interval hypothesis tests were also performed, which indicated that the true bias of all samples was less than ? 2 %. .

scholarly journals A Novel Use of 3-Methyl-2-Benzothiazolinone Hydrazone Hydrochloride Monohydrate for Kinetic Spectrophotometric Determination of Captopril in Pharmaceutical Formulations

2016 ◽  
Vol 71 ◽  
pp. 29-39
Author(s):  
Mouhammed Khateeb ◽  
Bashir Elias ◽  
Shahama Adi
Keyword(s):  
Correlation Coefficients ◽  
Pharmaceutical Formulations ◽  
Fixed Time ◽  
Rate Data ◽  
Significant Difference ◽  
Colored Product ◽  
Hydrochloride Monohydrate ◽  
Kinetic Spectrophotometric ◽  
Comparison Of The Results

A new, simple and sensitive kinetic spectrophotometric method has been proposed for the determination of captopril (CPT) in pharmaceutical formulations. The method is based on oxidation of 3-methyl-2-benzothiazolinone hydrazone hydrochloride monohydrate (MBTH) by ferric chloride followed by its coupling with the drug to form green-yellow colored product with absorbance maximum at 395nm. The concentration of CPT was calculated using the calibration equation for the rate data and fixed time methods. The linearity range was found to be 0.5–22.5 μg mL-1for each method. The correlation coefficients were 0.9994 and 0.9971 for rate data and fixed time methods respectively. The proposed methods were applied successfully for the determination of CPT in pharmaceutical formulations. Statistical comparison of the results shows that there is no significant difference between the proposed and official methods.

scholarly journals Quality of Ceftriaxone Sodium in Lyophilized Powder for Injection Evaluated by Clean, Fast, and Efficient Spectrophotometric Method

2017 ◽  
Vol 2017 ◽  
pp. 1-4 ◽  
Author(s):  
Patrícia Vidal de Aléssio ◽  
Ana Carolina Kogawa ◽  
Hérida Regina Nunes Salgado
Keyword(s):  
Spectrophotometric Method ◽  
Pharmaceutical Formulations ◽  
Uv Detector ◽  
Ceftriaxone Sodium ◽  
Accuracy And Precision ◽  
Relative Standard ◽  
Significant Difference ◽  
Gram Negative Organisms ◽  
Comparison Of The Results

Ceftriaxone sodium, an antimicrobial agent that plays an important role in clinical practice, is successfully used to treat infections caused by most Gram-positive and Gram-negative organisms. Since there are few rapid analytical methods for ceftriaxone analysis to use in the pharmaceutical routine, the aim of this research was to develop a new method able to quantify this cephalosporin. Therefore, a sensitive, rapid, simple UV spectrophotometric method for the determination and quantification of ceftriaxone sodium was proposed. The UV detector was set at 241 nm. Beer’s law obeyed the concentration range of 10–20 µg mL−1. Statistical comparison of the results with a well-established reported method showed excellent agreement and proved that there is no significant difference in the accuracy and precision. Intra- and interday variability for the method were less than 2% relative standard deviation. The proposed method was applied to the determination of the examined drugs in pharmaceutical formulations and the results demonstrated that the method is equally accurate, precise, and reproducible as the official methods.

Comparative Study for the Assay of Plant Derived Chemicals in Pharmaceutical Formulation Using Methods Dependent on Factorized Spectra

2021 ◽  
Author(s):  
Nesma M Fahmy ◽  
Adel M Michael
Keyword(s):  
Pharmaceutical Formulations ◽  
Pharmaceutical Formulation ◽  
Ternary Mixtures ◽  
Ratio Method ◽  
The Novel ◽  
Naturally Occurring ◽  
Accuracy And Precision ◽  
Validation Parameters ◽  
Significant Difference

Abstract Background Modern built-in spectrophotometer software supporting mathematical processes provided a solution for increasing selectivity for multicomponent mixtures. Objective Simultaneous spectrophotometric determination of the three naturally occurring antioxidants—rutin(RUT), hesperidin(HES), and ascorbic acid(ASC)—in bulk forms and combined pharmaceutical formulation. Method This was achieved by factorized zero order method (FZM), factorized derivative method (FD1M), and factorized derivative ratio method (FDRM), coupled with spectrum subtraction(SS). Results Mathematical filtration techniques allowed each component to be obtained separately in either its zero, first, or derivative ratio form, allowing the resolution of spectra typical to the pure components present in Vitamin C Forte® tablets. The proposed methods were applied over a concentration range of 2–50, 2–30, and 10–100 µg/mL for RUT, HES, and ASC, respectively. Conclusions Recent methods for the analysis of binary mixtures, FZM and FD1M, were successfully applied for the analysis of ternary mixtures and compared to the novel FDRM. All were revealed to be specific and sensitive with successful application on pharmaceutical formulations. Validation parameters were evaluated in accordance with the International Conference on Harmonization guidelines. Statistical results were satisfactory, revealing no significant difference regarding accuracy and precision. Highlights Factorized methods enabled the resolution of spectra identical to those of pure drugs present in mixtures. Overlapped spectra of ternary mixtures could be resolved by spectrum subtraction coupled FDRM (SS-FDRM) or by successive application of FZM and FD1M.

scholarly journals Kinetic Spectrophotometric Determination of Gemifloxacin Mesylate and Moxifloxacin Hydrochloride in Pharmaceutical Preparations Using 4-Chloro-7-nitrobenzo-2-oxa-1,3-diazole

2014 ◽  
Vol 2014 ◽  
pp. 1-12 ◽  
Author(s):  
Mohammed G. Abdel Wahed ◽  
Ragaa El Sheikh ◽  
Ayman A. Gouda ◽  
Sayed Abou Taleb
Keyword(s):  
Limit Of Detection ◽  
Kinetic Method ◽  
Pharmaceutical Preparations ◽  
Fixed Time ◽  
Spectrophotometric Methods ◽  
Relative Standard ◽  
Significant Difference ◽  
Kinetic Spectrophotometric ◽  
Comparison Of The Results

Simple, sensitive, and accurate kinetic spectrophotometric method was proposed for the determination of gemifloxacin mesylate (GMF) and moxifloxacin hydrochloride (MOX) in pure forms and pharmaceutical preparations (tablets). The method is based on coupling the studied drugs with 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole (NBD-Cl) in the presence of alkaline borate buffer. Spectrophotometric measurement was achieved by recording the absorbance at 466 and 464 nm for GMF and MOX, respectively, after a fixed time of 20 and 15 min on a water bath adjusted at 70 ± 5°C for both drugs. The different experimental parameters affecting the development and stability of the color were carefully studied and optimized. The absorbance-concentration plots were linear over the ranges 0.5–8.0 and 2.0–12 μg mL−1for GMF and MOX, respectively. The limit of detection of the kinetic method was about 0.12 (2.47 × 10−7 M) and 0.36 (8.22 × 10−7 M) μg mL−1for GMF and MOX, respectively. The proposed methods have been applied and validated successfully with percentage relative standard deviation (RSD% ≤ 0.52) as precision and percentage relative error (RE% ≤ 1.33) as accuracy. The robustness of the proposed method was examined with recovery values that were 97.5–100.5 ± 1.3–1.9%. Statistical comparison of the results with the reference spectrophotometric methods shows excellent agreement and indicates no significant difference in accuracy or precision.

scholarly journals  Determination of CLINDAMYCIN HCl in Capsules by New, Validated, Simple and Green Kinetic Spectrometric Method

2021 ◽  
Author(s):  
Shaza Affas ◽  
Amir Alhaj Sakur
Keyword(s):  
Initial Rate ◽  
Limit Of Detection ◽  
Reference Method ◽  
Fixed Time ◽  
Spectrometric Method ◽  
Iodide Ions ◽  
Kinetic Spectrophotometric ◽  
Optimized Conditions ◽  
Good Agreement

Abstract Background: simple, sensitive, free of organic solvents, kinetic spectrophotometric method has been developed for the determination of Clindamycin Hydrochloride, both in pure form and Capsules. Method used is based on reaction of Clindamycin with potassium iodide and potassium iodate in aqueous medium at (25 ±2 °c) to produce yellow colored tri iodide ions (I3-). the reaction is followed spectrophotometrically by measuring the absorbance at 350 nm wavelength during 40 minutes. Results: the effects of analytical parameters on reported kinetic methods were investigated. Under the optimized conditions, the initial rate and fixed time (at 10 min) methods were used for constructing the calibration graphs. The graphs were linear in concentration ranges 1-20 μg.ml-1 with limit of detection of 0.12 and 0.22 μg ml-1for the initial rate and fixed time methods, respectively. The results were satisfactory and the analytical performance for both methods was validated. Conclusion: The proposed methods have been applied to determine the components in capsules with an average recovery of 98.25% to 102.00% and the results are in good agreement with those found by the reference method.

scholarly journals Determination of penicillamine, tiopronin and glutathione in pharmaceutical formulations by kinetic spectrophotometry

2021 ◽  
Vol 71 (4) ◽  
pp. 619-630
Author(s):  
Lea Kukoc-Modun ◽  
Maja Biocic ◽  
Njegomir Radić
Keyword(s):  
Initial Rate ◽  
Low Cost ◽  
Pharmaceutical Formulations ◽  
Fixed Time ◽  
Calibration Graph ◽  
Simple Method ◽  
Kinetic Spectrophotometry ◽  
Rate Method ◽  
Time Ease

Abstract A novel and simple method for the determination of penicillamine (PEN), tiopronin (mercaptopropionyl glycine, MPG) and glutathione (GSH) in pharmaceutical formulations by kinetic spectrophotometry has been developed and validated. It is based on the redox reaction where the thiol compound (RSH) reduces CuII-neocuproine complex to CuI-neocuproine complex. The non-steady state signal of the formed CuI- neocuproine complex is measured at 458 nm. The initial rate and fixed time (at 1 min) methods were validated. The calibration graph was linear in the concentration range from 8.0 × 10‒7 to 8.0 × 10‒5 mol L−1 for the initial rate method and from 6.0 × 10‒7 to 6.0 × 10−5 mol L−1 for the fixed time method, with the detection limits of 2.4 × 10−7 and 1.4 × 10‒7 mol L−1, resp. Levels of PEN, MPG and GSH in pharmaceutical formulations were successfully assayed by both methods. The advantages of the presented methods include sensitivity, short analysis time, ease of application and low cost.

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