scholarly journals Synthesis and Antimicrobial Activity of a Silver-Hydroxyapatite Nanocomposite

2009 ◽  
Vol 2009 ◽  
pp. 1-6 ◽  
Author(s):  
Marcos Díaz ◽  
Flora Barba ◽  
Miriam Miranda ◽  
Francisco Guitián ◽  
Ramón Torrecillas ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Reduction Process ◽  
Bactericidal Effect ◽  
Gram Negative Bacteria ◽  
Subsequent Reduction ◽  
Visible Spectroscopy ◽  
High Antimicrobial Activity ◽  
Chemical Route ◽  
Uv Visible ◽  
High Degree

A silver-hydroxyapatite nanocomposite has been obtained by a colloidal chemical route and subsequent reduction process in H2/Ar atmosphere at350∘C. This material has been characterized by TEM, XRD, and UV-Visible spectroscopy, showing the silver nanoparticles (∼65 nm) supported onto the HA particles (∼130 nm) surface without a high degree of agglomeration. The bactericidal effect against common Gram-positive and Gram-negative bacteria has been also investigated. The results indicated a high antimicrobial activity forStaphylococcus aureus, PneumococcusandEscherichia coli,so this material can be a promising antimicrobial biomaterial for implant and reconstructive surgery applications.

Green Synthesis of Silver Nanoparticle Using Banana (Musa) Sap

2015 ◽  
Vol 1086 ◽  
pp. 7-10 ◽  
Author(s):  
S. Deena ◽  
Arun Dakshinamurthy ◽  
Paulraj Mosae Selvakumar
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Silver Nanoparticle ◽  
Gram Negative Bacteria ◽  
Visible Spectroscopy ◽  
High Antimicrobial Activity ◽  
Gram Positive Bacteria ◽  
Coated Fabric ◽  
Uv Visible

Green synthesis of silver nanoparticles using banana sap as a solvent, reducing agent and capping agent is reported in this work. Banana sap has also been used as a binder to incorporate silver nanoparticles into cotton fabric. UV-Visible spectroscopy was used to monitor the quantitative formation of silver nanoparticles. Silver nanoparticle coated fabric showed high antimicrobial activity against gram negative bacteria such asPseudomonas aureginosaandEscherichia coli, and gram positive bacteria such asBacillus subtilisandStaphylococcus aureus.KeywordsGreen synthesis, Silver nanoparticles, banana sap, antimicrobial activity

scholarly journals Antimicrobial Activity of Antibiotic Doped Carbon Nanoparticles Extracted from Kitchen Soot against Pathogenic Gram-negative Bacteria

2020 ◽  
pp. 1-8
Author(s):  
. Habiba ◽  
Israf Ud Din ◽  
S. Tasleem ◽  
Muhammad Mubashir ◽  
M. Afroz Bakht
Keyword(s):  
Antimicrobial Activity ◽  
Carbon Nanoparticles ◽  
Inhibition Zone ◽  
Dilution Method ◽  
Gram Negative Bacteria ◽  
Visible Spectroscopy ◽  
Carbon Nanoparticle ◽  
Gram Negative ◽  
Uv Visible ◽  
Antibacterial Potential

In this study carbon nanoparticles were extracted from kitchen soot and characterized by using UV/Visible spectroscopy.  Amoxicyline and vibramycin were doped on the carbon nanoparticles by mixing the solutions of the aforementioned antibiotics and carbon nanoparticles and then evaporated to dryness at 50°C. The antibacterial potential of carbon nanoparticle, antibiotics and carbon nanoparticles doped antibiotics were evaluated using Agar tub dilution method against two bacterial strain i.e. P. Aerogunis and Salmonella entrica. The result indicated that the carbon nanoparticle doped antibiotics showed marked increase in the antibacterial potential with inhibition zone of 16.2 mm against P. aerogunisa and 12.5 mm against Salmonela entrica for Vibramycin Doped CNPs. The inhibition zone of Amoxicyline doped carbon nanoparticle is 25.0 mm against P. aerogunisa and 30.0 mm against Salmonela entrica.

Disinfection of Fruits with Activated Water

2019 ◽  
Vol 10 ◽  
pp. 1864-1872
Author(s):  
Prof. Teodora P. Popova
Keyword(s):  
Antimicrobial Activity ◽  
Aqueous Solutions ◽  
Antimicrobial Properties ◽  
The Other ◽  
Gram Negative Bacteria ◽  
High Antimicrobial Activity ◽  
Gram Negative ◽  
E Coli ◽  
Number Of Microorganisms ◽  
Lower Effect

The effect of ionized aqueous solutions (anolytes and catholyte) in the processing of fruits (cherries, morellos, and strawberries) for decontamination has been tested. Freshly prepared analytes and catholyte without the addition of salts were used, as well as stored for 7 months anolytes, prepared with 0.5% NaCl and a combination of 0.5% NaCl and 0.5% Na2CO3. The anolyte prepared with a combination of 0.5% NaCl and 0.5% Na2CO3, as well as the anolyte obtained with 0.5% NaCl, exhibit high antimicrobial activity against the surface microflora of strawberries, cherries, and sour cherries. They inactivate E. coli for 15 minutes. The other species of the fam. Enterobacteriaceae were also affected to the maximum extent, as is the total number of microorganisms, especially in cherries and sour cherries. Even stored for 7 months, they largely retain their antimicrobial properties. Anolyte and catholyte, obtained without the addition of salts, showed a lower effect on the total number of microorganisms, but had a significant effect on Gram-negative bacteria, and especially with regard to the sanitary indicative E. coli.

Optimization of Silver Nanoparticles Production using Aspergillus niger and Study of Antimicrobial Activity

2021 ◽  
Vol 12 (4) ◽  
pp. 2383-2388
Author(s):  
Suguna Selvakumaran ◽  
Kayathri Marimuthu ◽  
Thiruvany Poopalan ◽  
Kalaiyarasi Tamil Selvan ◽  
Nozieana Khairuddin
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Aspergillus Niger ◽  
Culture Supernatant ◽  
Gram Negative Bacteria ◽  
Mixing Ratio ◽  
Zone Of Inhibition ◽  
Gram Positive Bacteria ◽  
Physiochemical Parameters ◽  
Uv Visible

Silver nanoparticles have attracted high attention worldwide for their various applications. The physiochemical parameters such as temperature, media, mixing ratio affect the rate of synthesis of silver nanoparticles and their yield. Thus, optimization of these physiochemical parameters is needed to enhance the production of silver nanoparticles. In this study, silver nanoparticles were synthesized using Aspergillus niger culture supernatant. The produced silver nanoparticles were characterized using UV-visible Spectrophotometer at 200 nm to 700 nm, which had a peak at 450 nm, indicates the formation of silver nanoparticles. It was found that Sabouraud Dextrose Broth (SDB) as optimum media, 40 ml of supernatant and 10 ml of silver nitrate as optimum mixing ratio and 65°C as optimum temperature to produce silver nanoparticles. The optimized silver nanoparticles were subjected to antimicrobial activity, and it was found that it is highly effective towards gram-negative bacteria than gram-positive bacteria where the zone of inhibition for Escherichia coli was  7 ± 2.7 mm and 5.3 ± 2.1 mm for Staphylococcus aureus.

scholarly journals Structural Characterization, Antimicrobial Activity, and In Vitro Cytotoxicity Effect of Black Seed Oil

2019 ◽  
Vol 2019 ◽  
pp. 1-9 ◽  
Author(s):  
Sewara J. Mohammed ◽  
Hassan H. H. Amin ◽  
Shujahadeen B. Aziz ◽  
Aram M. Sha ◽  
Sarwar Hassan ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Structural Characterization ◽  
Seed Oil ◽  
Double Bonds ◽  
Visible Spectroscopy ◽  
Cytotoxic Effects ◽  
Black Seed ◽  
Uv Visible ◽  
Black Seed Oil

This study was aimed to investigate the structure of bioactive components of black seed oil (BSO) and their antimicrobial and cytotoxic effects. Initially, the structural examination was conducted using various spectroscopic techniques, such as FTIR, TLC, and UV-visible spectroscopy, which are important in determining substituents, functional groups, and the presence of conjugated double bonds in BSO. From the FTIR spectra, a variety of sharp, strong, and weak peaks were specified relating to the main components of thymoquinone (TQ), dithymoquinone, thymohydroquinone, and thymol in BSO. The results of UV-visible spectroscopy confirmed the presence of thymoquinone as a major compound, and conjugated double bonds were also found. In addition, qualitative TLC analysis was used to identify thymoquinone from the methanol-extracted layer in BSO, by calculating the retention factor (Rf) value. Furthermore, antimicrobial activity of BSO was studied against various types of bacteria. Strong bacterial inhibitory effects were observed, especially against Bacillus subtilis, with an average inhibition zone of 15.74 mm. Moreover, through the use of the MTT assay in vitro, it was shown that BSO does not exhibit any cytotoxicity towards human peripheral blood mononuclear cells (PBMCs). It was also found from the structural characterization of BSO that the existence of TQ is responsible for potential antibacterial activity without any cytotoxic effects. The main observation of this work is that BSO has antimicrobial activity even against methicillin-resistant Staphylococcus aureus (MRSA).

scholarly journals Investigation of the Antimicrobial Activity of Some Streptomyces spp. Isolated from Nineveh Province

2010 ◽  
Vol 4 (2) ◽  
pp. 24-32
Author(s):  
M. B. I. Kassim ◽  
S. S. Eleya
Keyword(s):  
Antimicrobial Activity ◽  
Active Compound ◽  
Bioactive Compound ◽  
Gram Negative Bacteria ◽  
High Antimicrobial Activity ◽  
Biochemical Tests ◽  
Uv Spectrum ◽  
Gram Negative ◽  
Streptomyces Spp ◽  
Streptomyces Lavendulae

Atotal of 62 different Streptomyces isolates were recovered from 17 samples of soil collected from different sites of Nineveh Province. Only 23 isolates showed activity against test Gram-positive and Gram-negative bacteria. One isolate showed high antimicrobial activity against Staphylococcus aureus, was selected and identified as Streptomyces lavendulae on the bases of microscopic, morphologic, biochemical tests and its sensitivity to some antibiotics. The bioactive compound produced by S. lavendulae was isolated on TLC plate (Rf 0.85). The UV spectrum of the active compound in methanol showed one peak at 280 nm. From these data it could be concluded that the active compound probably belongs to macrolide antibiotics group.

scholarly journals Biosynthesis and Characterisation of Silver Nanoparticles using Leaf Extract of Achras sapota l. for its Antimicrobial Activity

2020 ◽  
pp. 246-256
Author(s):  
Maphibanri Maring ◽  
Akila Elias ◽  
V. B. Narayanaswamy
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Leaf Extract ◽  
Colour Change ◽  
Visible Spectroscopy ◽  
Sem And Tem ◽  
Achras Sapota ◽  
Uv Visible ◽  
Biosynthesized Agnps ◽  
Ethanolic Leaf Extract

<p>Nanotechnology is a field that is rapidly growing, making an impact in all spheres of human life. In the current study, silver nanoparticles were synthesized using the ethanolic leaf extract of <em>Achras sapota. </em>Characterization was carried out using UV-Visible spectroscopy, FTIR, XRD, SEM and TEM. The formation of AgNPs was confirmed through UV-Visible spectroscopy by the colour change. Based on the XRD pattern, the crystalline property of the AgNPs was established. The functional group present in the ethanolic leaf extract of <em>Achras sapota</em> is responsible for the reduction of the Ag<sup>+</sup> ion which was studied through FTIR. From the SEM and TEM analysis, it was found that the formed nanoparticles are spherical in shape and nano in size. The biosynthesized AgNPs was evaluated for its antimicrobial activity against gram positive bacteria (<em>S. aureus and L. bacillus</em>) and gram negative bacteria (<em>E. coli and P. aeruginosa</em>) using disc diffusion for preliminary screening of antimicrobial activity and dilution method for evaluation of antibacterial effectiveness and effect of silver nanoparticles on bacterial growth and it was found to exhibit potential antimicrobial activity. The biosynthesized AgNPs was found to be efficient in terms of reaction time as well as stability, eco-friendly and cost effective.</p>

scholarly journals Novel moisturized and antimicrobial hand gel based on zinc-aminoclay and Opuntia humifusa extract

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Hien Thi Hoang ◽  
Vinh Van Tran ◽  
Vu Khac Hoang Bui ◽  
Oh-Hyeok Kwon ◽  
Ju-Young Moon ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Skin Irritation ◽  
Gram Negative Bacteria ◽  
Skin Protection ◽  
Toxicity Evaluation ◽  
High Antimicrobial Activity ◽  
Gram Positive Bacteria ◽  
Opuntia Humifusa ◽  
Standard Level ◽  
Low Toxicity

AbstractThe high antimicrobial ability and low toxicity of zinc-aminoclay (ZnAC) are claimed in our previous reports. In this study, we formulate a novel hand gel based on ZnAC and Opuntia humifusa (O. humifusa) extract, which is a high moisturizing agent. The antimicrobial activity, cytotoxicity, moisturizing effect, and clinical skin irritation of the hand gel are evaluated. The hand gel with 0.5 wt.% ZnAC and 1.0 v/v% O. humifusa extract can kill more than 99% Escherichia coli (gram-negative bacteria) and Staphylococcus aureus (gram-positive bacteria) after 24 h. Toxicity evaluation shows that, the hand gel does not affect the viability of mammalian HaCaT cells. Additionally, skin moisture is increased by applying the hand gel while its viscosity is at the standard level of commercial products. The hand gel has a skin irritation index of 0.0 and is classified as a non-irritating product. We successfully formulated hand gel from ZnAC, glucomannan, glycerol, and O. humifusa extract. Owing to the high antimicrobial activity and skin protection of hand gels, they are suitable to be used as hand sanitizers in restaurants, hospitals, and homes effectively.

scholarly journals Composition and Antimicrobial Analysis of the Essential Oil of Litsea Laevigata Nees. (Lauraceae)

2008 ◽  
Vol 3 (7) ◽  
pp. 1934578X0800300 ◽  
Author(s):  
Arif M Muhammed ◽  
Raj M Subbu ◽  
Leopold Jirovetz ◽  
Shafi P Mohamed
Keyword(s):  
Antimicrobial Activity ◽  
Aspergillus Niger ◽  
Essential Oil ◽  
Western Ghats ◽  
Klebsiella Aerogenes ◽  
Gram Negative Bacteria ◽  
Proteus Vulgaris ◽  
High Antimicrobial Activity ◽  
Gram Positive ◽  
Gram Positive Bacteria

The essential oil of the berries of Litsea laevigata Nees., growing wild in Western Ghats, Kerala, India, was obtained by hydrodistillation and was fractionated by passage through a column using n-pentane and diethyl ether as eluents. The essential oil and its fractions were analysed by GC and GC-MS. Twenty seven compounds representing 99.2% of the original oil were identified. The major components are the terpene hydrocarbons trans-α-bergamotene(26.7%), α-pinene (25%) and β-pinene (8.2%). The antimicrobial activity of the essential oil and its fractions against four Gram-positive and four Gram-negative bacteria ( Staphylococcus aureus, Bacillus subtilis, Streptococcus faecalis, Staphylococcus albus, Escherichia coli, Pseudomonas aeruginosa, Proteus vulgaris, Klebsiella aerogenes), as well as two fungi ( Candida albicans, Aspergillus niger) was studied. The bioassay showed that the oil exhibited moderate to high antimicrobial activity, being very active against gram-positive bacteria, such as Streptococcus albus, and the fungus Aspergillus niger.

scholarly journals Biological Activity and Composition of the Essential Oil of Tetrataenium Nephrophyllum (Apiaceae) from Iran

2007 ◽  
Vol 2 (12) ◽  
pp. 1934578X0700201 ◽  
Author(s):  
Ali Sonboli ◽  
Mohammad Reza Kanani ◽  
Morteza Yousefzadi ◽  
Mehran Mojarrad
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oil ◽  
Gram Negative Bacteria ◽  
Flowering Stage ◽  
High Antimicrobial Activity ◽  
Aerial Parts ◽  
Sesquiterpene Hydrocarbons ◽  
Germacrene D ◽  
Aliphatic Esters ◽  
Total Oil

The aerial parts of Tetrataenium nephrophyllum were collected at the flowering stage, hydrodistilled, and the essential oil was analyzed by GC and GC-MS. Forty components accounting for 97.9% of the total oil were identified. Germacrene D (38.5%), 2-ethylhexyl acetate (11.2%), n-octyl 2-methylbutanoate (9.2%) and geranyl isovalerate (8.3%) were the major constituents. Sesquiterpene hydrocarbons (51.3%) and aliphatic esters (40.4%) were found to be the main group of compounds. The antimicrobial activity of the essential oil of T. nephrophyllum was determined against seven Gram-positive and Gram-negative bacteria (Bacillus subtilis, Enterococcus faecalis, Staphylococcus aureus, S. epidermidis, Escherichia coli, Pseudomonas aeruginosa and Klebsiella pneumoniae), as well as three fungi (Candida albicans, Saccharomyces cerevisiae and Aspergillus niger). The bioassay showed that the oil exhibited moderate to high antimicrobial activity.

Sign in / Sign up

Export Citation Format

Share Document