scholarly journals Characterization ofBacillus subtilissporulation and bacteriophage infection via FT-IR spectroscopy

2009 ◽  
Vol 23 (3-4) ◽  
pp. 165-174
Author(s):  
Tami H. Mysliwiec ◽  
Anthony F. Tierno ◽  
David J. Aurentz
Keyword(s):  
Spectral Analysis ◽  
Ir Spectroscopy ◽  
Attenuated Total Reflectance ◽  
Wild Type ◽  
Total Reflectance ◽  
Bacteriophage Infection ◽  
Aliphatic Ester ◽  
Cellular Population ◽  
Ft Ir ◽  
Ft Ir Spectroscopy

A comparative analysis on cultures from wild-type and sporulation mutants ofBacillus subtilisinfected with pseudotemperate spore-converting bacteriophages was performed to discern differences in the chemical functionality of the cellular population. Variations in the cell surface chemistry ofB. subtiliscultures were analyzed using attenuated total reflectance (ATR) Fourier transform infrared (FT-IR) spectroscopy of dried sample films on optical plates. Comparison of wild-type and phage infected cultures revealed an increase in the amount of aliphatic ester in the infected populations. Spectral analysis of cultures from bacteriophage infected sporulation mutants,spo0Kandspo0Jrevealed varying levels of ester linkages.

scholarly journals Chemometric analysis of attenuated total reflectance infrared spectra ofProteus mirabilisstrains with defined structures of LPS

2016 ◽  
Vol 22 (5) ◽  
pp. 325-335 ◽  
Author(s):  
Paulina Zarnowiec ◽  
Andrzej Mizera ◽  
Magdalena Chrapek ◽  
Mariusz Urbaniak ◽  
Wieslaw Kaca
Keyword(s):  
Ir Spectroscopy ◽  
Infrared Region ◽  
Attenuated Total Reflectance ◽  
Mid Infrared ◽  
Total Reflectance ◽  
Typing Methods ◽  
Ft Ir ◽  
Tract Infections ◽  
Ft Ir Spectroscopy

Proteus spp. strains are some of the most important pathogens associated with complicated urinary tract infections and bacteremia affecting patients with immunodeficiency and long-term urinary catheterization. For epidemiological purposes, various molecular typing methods have been developed for this pathogen. However, these methods are labor intensive and time consuming. We evaluated a new method of differentiation between strains. A collection of Proteus spp. strains was analyzed by attenuated total reflectance Fourier transform infrared (ATR FT-IR) spectroscopy in the mid-infrared region. ATR FT-IR spectroscopy used in conjunction with a diamond ATR accessory directly produced the biochemical profile of the surface chemistry of bacteria. We conclude that a combination of ATR FT-IR spectroscopy and mathematical modeling provides a fast and reliable alternative for discrimination between Proteus isolates, contributing to epidemiological research.

scholarly journals Analysis of ‘legal high’ substances and common adulterants using handheld spectroscopic techniques

2015 ◽  
Vol 7 (2) ◽  
pp. 736-746 ◽  
Author(s):  
S. Assi ◽  
A. Guirguis ◽  
S. Halsey ◽  
S. Fergus ◽  
J. L. Stair
Keyword(s):  
Fourier Transform ◽  
Ir Spectroscopy ◽  
Near Infrared ◽  
Fourier Transform Infrared ◽  
Attenuated Total Reflectance ◽  
Spectroscopic Techniques ◽  
Total Reflectance ◽  
Legal High ◽  
Ft Ir ◽  
Ft Ir Spectroscopy

Three handheld spectrometers, near-infrared (NIR), Raman and attenuated total reflectance Fourier transform-infrared (ATR-FT-IR) spectroscopy, were used for the identification of ‘legal high’ model mixtures and Internet products.

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