scholarly journals Pinellia ternata,Citrus reticulata, and Their Combinational Prescription Inhibit Eosinophil Infiltration and Airway Hyperresponsiveness by Suppressing CCR3+ and Th2 Cytokines Production in the Ovalbumin-Induced Asthma Model

2009 ◽  
Vol 2009 ◽  
pp. 1-10 ◽  
Author(s):  
In-Soo Ok ◽  
Seung-Hyung Kim ◽  
Bok-Kyu Kim ◽  
Jang-Cheon Lee ◽  
Young-Cheol Lee
Keyword(s):  
Murine Model ◽  
Airway Hyperresponsiveness ◽  
Immunoglobulin E ◽  
Allergic Inflammation ◽  
Eosinophil Infiltration ◽  
Citrus Reticulata ◽  
Th2 Cytokines ◽  
Pinellia Ternata ◽  
Th2 Cytokine ◽  
Histamine Production

Background and Objective. This study was aimed to analyse the curative effects ofPinellia ternata,Citrus reticulata, and their combination on airway hyperresponsiveness (AHR) to inhaled methacholine, pulmonary eosinophilic infiltration, Th2 cytokine production, and IgE and histamine production in a murine model of asthma.Methods. For this purpose, BALB/c mice were systemically sensitized to ovalbumin (OVA) followed intratracheally, intraperitoneally, and by aerosol allergen challenges for 12 weeks. We examined the development of pulmonary eosinophilic accumulation, control of Th2 cytokine, immunoglobulin E (IgE), and histamine productions in a murine model of asthma.Results. Our data suggest that the therapeutic mechanism by whichPinellia ternata,Citrus reticulata, and their combinational prescription effectively treats asthma is based on reductions of eosinophil infiltration, eotaxin receptor (CCR3), histamine, OVA-specific IgE productions in serum, and Th2 cytokines (IL-5, IL-13) by marked reductions of IL-5 and IL-13 mRNA expression in lung tissue.Conclusions. These findings provide evidence thatPinellia ternata,Citrus reticulata, and their combination play a regulatory role in allergic inflammation and offer therapeutic approaches as novel CCR3 antagonists for treatment asthma. However, it is not clear whether pharmacological activities of prescription composed of two herbs are potentiated due to synergistic effect or additive effect.

scholarly journals Th2 cytokines-DUOX2-ROS-HMGB1 translocation axis is important in the pathogenesis of allergic rhinitis

2021 ◽  
Vol 135 (3) ◽  
pp. 483-494
Author(s):  
Hyun Jin Min ◽  
Joon Soon Park ◽  
Kyung Soo Kim ◽  
Seung Yong Park ◽  
Honghwan Choi ◽  
...  
Keyword(s):  
Allergic Rhinitis ◽  
Murine Model ◽  
Immunoglobulin E ◽  
Periodic Acid ◽  
High Mobility ◽  
Upper Airway ◽  
Th2 Cytokines ◽  
Periodic Acid Schiff ◽  
Th2 Cytokine

Abstract The function of high-mobility group box 1 (HMGB1) varies according to its location. However, the translocation mechanism behind HMGB1 remains unclear. We hypothesize that type 2 helper T cell (Th2) cytokines are involved in the translocation of HMGB1 in the upper airway epithelium. We investigated the mechanism behind HMGB1 translocation using Th2 cytokine stimulation and examined the clinical significance of HMGB1 translocation in allergic rhinitis (AR). Cytoplasmic and extracellular HMGB1 were increased in AR. Inhibiting HMGB1 translocation with glycyrrhizic acid (GA) decreased the level of antigen-specific immunoglobulin E (IgE), the degree of Periodic Acid–Schiff (PAS), and Sirius Red staining in the murine model. The in vivo reactive oxygen species (ROS) level in the nasal mucosa was higher in the mice with AR than in the controls. Th2 cytokine-induced up-regulation of the ROS and translocation of HMGB1 by Th2 cytokines was dependent on the generated ROS. The ROS level also increased in the murine model. We suggest that the Th2 cytokine-dual oxidase (DUOX)2-ROS-HMGB1 translocation axis is important in AR pathogenesis.

scholarly journals Antiasthmatic Effects of Hesperidin, a Potential Th2 Cytokine Antagonist, in a Mouse Model of Allergic Asthma

2011 ◽  
Vol 2011 ◽  
pp. 1-12 ◽  
Author(s):  
Seung-Hyung Kim ◽  
Bok-Kyu Kim ◽  
Young-Cheol Lee
Keyword(s):  
Mouse Model ◽  
Airway Inflammation ◽  
Airway Hyperresponsiveness ◽  
Immune Cell ◽  
Airflow Obstruction ◽  
Specific Ige ◽  
Eosinophil Infiltration ◽  
Th2 Cytokines ◽  
Th2 Cytokine

Background and Objective. The features of asthma are airway inflammation, reversible airflow obstruction, and an increased sensitivity to bronchoconstricting agents, termed airway hyperresponsiveness (AHR), excess production of Th2 cytokines, and eosinophil accumulation in the lungs. To investigate the antiasthmatic potential of hesperidin as well as the underlying mechanism involved, we studied the inhibitory effect and anti-inflammatory effect of hesperidin (HPN) on the production of Th2 cytokines, eotaxin, IL-17, -OVA-specific IgEin vivoasthma model mice.Methods. In this paper, BALB/c mice were systemically sensitized to ovalbumin (OVA) followed intratracheally, intraperitoneally, and by aerosol allergen challenges. We investigated the effect of HPN on airway hyperresponsiveness, pulmonary eosinophilic infiltration, various immune cell phenotypes, Th2 cytokine production and OVA-specific IgE production in a mouse model of asthma.Results. In BALB/c mice, we found that HPN-treated groups had suppressed eosinophil infiltration, allergic airway inflammation, and AHR, and these occurred by suppressing the production of IL-5, IL-17, and OVA-specific IgE.Conclusions. Our data suggest that the therapeutic mechanism by which HPN effectively treats asthma is based on reductions of Th2 cytokines (IL-5), eotaxin, OVA-specific IgE production, and eosinophil infiltration via inhibition of GATA-3 transcription factor.

Dissociation of Airway Hyperresponsiveness from Immunoglobulin E and Airway Eosinophilia in a Murine Model of Allergic Asthma

1999 ◽  
Vol 20 (6) ◽  
pp. 1326-1334 ◽  
Author(s):  
Julie A. Wilder ◽  
D.David S. Collie ◽  
Bridget S. Wilson ◽  
David E. Bice ◽  
C. Richard Lyons ◽  
...  
Keyword(s):  
Allergic Asthma ◽  
Murine Model ◽  
Airway Hyperresponsiveness ◽  
Immunoglobulin E ◽  
Airway Eosinophilia

scholarly journals Illicium verum Extract and Trans-Anethole Attenuate Ovalbumin-Induced Airway Inflammation via Enhancement of Foxp3+ Regulatory T Cells and Inhibition of Th2 Cytokines in Mice

2017 ◽  
Vol 2017 ◽  
pp. 1-12 ◽  
Author(s):  
Yoon-Young Sung ◽  
Seung-Hyung Kim ◽  
Dong-Seon Kim ◽  
Ji-eun Lee ◽  
Ho Kyoung Kim
Keyword(s):  
T Cells ◽  
Regulatory T Cells ◽  
Airway Inflammation ◽  
Airway Hyperresponsiveness ◽  
Cytokine Production ◽  
Foxp3 Expression ◽  
Eosinophilic Infiltration ◽  
Th2 Cytokines ◽  
Th2 Cytokine ◽  
Illicium Verum

Illicium verum is used in traditional medicine to treat inflammation. The study investigates the effects of IVE and its component, trans-anethole (AET), on airway inflammation in ovalbumin- (OVA-) induced asthmatic mice. Asthma was induced in BALB/c mice by systemic sensitization to OVA, followed by intratracheal, intraperitoneal, and aerosol allergen challenges. IVE and AET were orally administered for four weeks. We investigated the effects of treatment on airway hyperresponsiveness, IgE production, pulmonary eosinophilic infiltration, immune cell phenotypes, Th2 cytokine production in bronchoalveolar lavage, Th1/Th2 cytokine production in splenocytes, forkhead box protein 3 (Foxp3) expression, and lung histology. IVE and AET ameliorated OVA-driven airway hyperresponsiveness (p<0.01), pulmonary eosinophilic infiltration (p<0.05), mucus hypersecretion (p<0.01), and IL-4, IL-5, IL-13, and CCR3 production (p<0.05), as well as IgE levels (p<0.01). IVE and AET increased Foxp3 expression in lungs (p<0.05). IVE and AET reduced IL-4 and increased IFN-γ production in the supernatant of splenocyte cultures (p<0.05). Histological studies showed that IVE and AET inhibited eosinophilia and lymphocyte infiltration in lungs (p<0.01). These results indicate that IVE and AET exert antiasthmatic effects through upregulation of Foxp3+ regulatory T cells and inhibition of Th2 cytokines, suggesting that IVE may be a potential therapeutic agent for allergic lung inflammation.

scholarly journals The Ethanol Extract of Holotrichia diomphalia Larvae, Containing Fatty acids and Amino acids, Exerts Anti-Asthmatic Effects through Inhibition of the GATA-3/Th2 Signaling Pathway in Asthmatic Mice

Molecules ◽  
2019 ◽  
Vol 24 (5) ◽  
pp. 852 ◽  
Author(s):  
Jung-Hee Hong ◽  
Seung-Hyung Kim ◽  
Young-Cheol Lee
Keyword(s):  
Allergic Asthma ◽  
Immunoglobulin E ◽  
Ethanol Extract ◽  
Allergic Diseases ◽  
Eosinophil Infiltration ◽  
Th2 Cytokines ◽  
Cell Hyperplasia ◽  
Vitro Assay ◽  
Anti Inflammatory

Holotrichia diomphalia larvae (HD), a natural product from an insect resource, possesses many pharmacological properties, including anticoagulant, antitumor, anti-inflammatory, and analgesic activity. The major bioactive ingredients include oleic acid, palmitic acid, palmitoleic acid, linoleic acid, proline, and glutamic acid. Although HD is associated with immunoregulatory activities in allergic diseases, the therapeutic mechanisms of the action of HD in allergic diseases have not been investigated. The aim of this study was to evaluate the anti-asthmatic potential of HD in an ovalbumin (OVA)-induced mouse model of allergic asthma. Moreover, the anti-inflammatory potential of HD was examined to identify a plausible mechanism of action of HD in vitro. HD strongly reduced goblet cell hyperplasia, eosinophil infiltration, and reactive oxygen species (ROS), which reduced airway hyperresponsiveness (AHR), inflammation, and the expression of Th2 cytokines (IL-5 and IL-13) in bronchoalveolar lavage fluid (BALF). The expression of IL-5, IL-4, eotaxin-2, lysyl oxidase-like 2 (loxl2), and GATA-binding protein 3 (GATA-3) was attenuated in the lungs. In an in vitro assay, HD exerted immunomodulatory effects through the suppression of Th2 cytokines (IL-5, IL-13), IL-17, and tumor necrosis factor (TNF)-α production through downregulation of GATA-3 expression in EL-4 T cells. These findings suggest that the anti-asthmatic activity of HD may occur through the suppression of Th2 cytokines and total Immunoglobulin E (IgE) production by inhibition of the GATA-3 transcription pathway. Our results suggest that HD may be a potential alternative therapy, or a novel therapeutic traditional medicine, for the treatment of allergic asthma.

scholarly journals Antiasthmatic Effects of Herbal Complex MA and Its Fermented Product MA128

2012 ◽  
Vol 2012 ◽  
pp. 1-12 ◽  
Author(s):  
Dong-Seon Kim ◽  
Seung-Hyung Kim ◽  
Bok-Kyu Kim ◽  
Min Cheol Yang ◽  
Jin Yeul Ma
Keyword(s):  
Lactobacillus Acidophilus ◽  
Airway Hyperresponsiveness ◽  
Cytokine Production ◽  
Immune Cell ◽  
Allergic Airway Inflammation ◽  
Lavage Fluid ◽  
Specific Ige ◽  
Eosinophil Infiltration ◽  
Th2 Cytokine ◽  
Fermented Product

This study was conducted to determine if oral administration of the novel herbal medicine, MA, and itsLactobacillus acidophilusfermented product, MA128, have therapeutic properties for the treatment of asthma. Asthma was induced in BALB/c mice by systemic sensitization to ovalbumin (OVA) followed by intratracheal, intraperitoneal, and aerosol allergen challenges. MA and MA128 were orally administered 6 times a week for 4 weeks. At 1 day after the last ovalbumin exposure, airway hyperresponsiveness was assessed and samples of bronchoalveolar lavage fluid, lung cells, and serum were collected for further analysis. We investigated the effect of MA and MA128 on airway hyperresponsiveness, pulmonary eosinophilic infiltration, various immune cell phenotypes, Th2 cytokine production, OVA-specific IgE production, and Th1/Th2 cytokine production in this mouse model of asthma. In BALB/c mice, we found that MA and MA128 treatment suppressed eosinophil infiltration into airways and blood, allergic airway inflammation and AHR by suppressing the production of IL-5, IL-13, IL-17, Eotaxin, and OVA-specific IgE, by upregulating the production of OVA-specific Th1 cytokine (IFN-γ), and by downregulating OVA-specific Th2 cytokine (IL-4) in the culture supernatant of spleen cells. The effectiveness of MA was increased by fermentation withLactobacillus acidophilus.

scholarly journals Th2 cells and macrophages cooperatively induce allergic inflammation through histamine signaling

PLoS ONE ◽  
2021 ◽  
Vol 16 (3) ◽  
pp. e0248158
Author(s):  
Naruhito Iwasaki ◽  
Seigo Terawaki ◽  
Kouhei Shimizu ◽  
Daisuke Oikawa ◽  
Hirokazu Sakamoto ◽  
...  
Keyword(s):  
Mouse Model ◽  
Allergic Inflammation ◽  
Allergic Diseases ◽  
Th2 Cells ◽  
Th2 Cytokines ◽  
Receptor Signaling ◽  
Alternatively Activated Macrophages ◽  
Th2 Cell ◽  
Histamine Production

Histamine, which is mainly produced by mast cells and basophils, participates in various allergic symptoms, and some studies have reported that macrophages also produce histamine. Moreover, recent studies have revealed that macrophages, especially alternatively activated macrophages (M2) induced by T helper 2 (Th2) cytokines, such as interleukin (IL)-4 and IL-13, participate in the pathogenesis of allergic diseases. The major source of Th2 cytokines is antigen-specific Th2 cells. To elucidate the relationship between histamine, macrophages, and Th2 cells in allergic inflammation, we established a macrophage-Th2 cell co-culture model in vitro and an antigen-specific Th2 cell transfer mouse model of rhinitis. In vitro analyses indicated that macrophages produce histamine by interacting with antigen-specific Th2 cells through the antigen. Furthermore, Th2 cells and macrophages cooperatively elicited rhinitis in the mouse model. We determined that histamine induces Th2- and macrophage-elicited sneezing responses through H1 receptor signaling, whereas it induces nasal eosinophil infiltrations through H4 receptor signaling. Collectively, these results indicate a novel histamine production mechanism by macrophages, in which Th2 cells and macrophages cooperatively induce nasal allergic inflammation through histamine signaling.

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