scholarly journals Effects of Radix Adenophorae and Cyclosporine A on an OVA-Induced Murine Model of Asthma by Suppressing to T Cells Activity, Eosinophilia, and Bronchial Hyperresponsiveness

2008 ◽  
Vol 2008 ◽  
pp. 1-11 ◽  
Author(s):  
Seong-Soo Roh ◽  
Seung-Hyung Kim ◽  
Young-Cheol Lee ◽  
Young-Bae Seo
Keyword(s):  
T Cells ◽  
Lung Tissue ◽  
Cyclosporine A ◽  
Murine Model ◽  
Flow Cytometric Analysis ◽  
Bronchial Hyperresponsiveness ◽  
Enzyme Linked Immunosorbent Assay ◽  
Inhibitory Effects ◽  
Lung Weight ◽  
Flow Cytometric

The present study is performed to investigate the inhibitory effects of Radix Adenophorae extract (RAE) on ovalbumin-induced asthma murine model. To study the anti-inflammatory and antiasthmatic effects of RAE, we examined the development of pulmonary eosinophilic inflammation and inhibitory effects of T cells in murine by RAE and cyclosporine A (CsA). We examined determination of airway hyperresponsiveness, flow cytometric analysis (FACS), enzyme-linked immunosorbent assay (ELISA), quantitative real time (PCR), hematoxylin-eosin staining, and Masson trichrome staining in lung tissue, lung weight, total cells, and eosinophil numbers in lung tissue. We demonstrated how RAE suppressed development on inflammation and decreased airway damage.

scholarly journals Human Articular Chondrocytes Induce Interleukin-2 Nonresponsiveness to Allogeneic Lymphocytes

Cartilage ◽  
2016 ◽  
Vol 8 (3) ◽  
pp. 300-306 ◽  
Author(s):  
Satomi Abe ◽  
Hitoshi Nochi ◽  
Hiroshi Ito
Keyword(s):  
T Cells ◽  
Articular Chondrocytes ◽  
Interleukin 2 ◽  
Flow Cytometric Analysis ◽  
Third Party ◽  
Soluble Form ◽  
Enzyme Linked Immunosorbent Assay ◽  
Dependent Manner ◽  
Activated T Cells ◽  
Allogeneic Lymphocytes

Introduction We previously showed that articular chondrocytes (ACs) have immune privilege and immunomodulatory functions like those of mesenchymal stem cells. To elucidate these mechanisms, we focused on interleukin-2 (IL-2), which plays critical roles in lymphocyte mitogenic activity. The purpose of this study was to explore whether ACs affect the role of IL-2 underlying immunomodulatory functions. Material and Methods Irradiated human ACs from osteoarthritis donors were used. Third-party ACs were added to the mixed lymphocyte reaction (MLR) with or without recombinant human IL-2 (rhIL-2), and the levels of IL-2 and the soluble form of the IL-2 receptor α (sIL-2Rα) protein in supernatant were measured by enzyme-linked immunosorbent assay. Recombinant human IL-2 (rhIL-2) was also added to the MLR. To detect the expression of IL-2 receptor α (CD25) on lymphocytes in the MLR, flow cytometric analysis was performed. Last, ACs and allogeneic activated CD4+ T cell were co-cultured, and the expression of CD25 on activated T cells was examined by flow cytometry. Results Third-party ACs significantly inhibited the MLR and reduced the level of sIL-2Rα in a dose-dependent manner, but did not affect the concentration of IL-2. Exogenous rhIL-2 accelerated MLR but did not rescue the inhibitory effect of ACs. ACs inhibited the expression of CD25 on activated CD4+ T cells. Discussion Our results showed that third-party ACs inhibited the proliferation of allogeneic activated lymphocytes, thereby inhibiting production sIL-2Rα, although ACs did not affect IL-2 secretion from lymphocytes. Also, ACs inhibited CD25 expression on activated CD4+ T cells. Thus, ACs inhibited the immune response of allogeneic lymphocytes by inducing IL-2 nonresponsiveness.

Flow cytometric analysis of intracellular IL-17A in T-cells during ectromelia virus infection

2012 ◽  
Vol 55 (04) ◽  
pp. 367-369 ◽  
Author(s):  
L. SZULC ◽  
M. GIERYŃSKA ◽  
A. BORATYŃSKA ◽  
L. MARTYNISZYN ◽  
M. NIEMIAŁTOWSKI
Keyword(s):  
T Cells ◽  
Virus Infection ◽  
Flow Cytometric Analysis ◽  
Ectromelia Virus ◽  
Flow Cytometric

scholarly journals High frequency of circulating HBcAg-specific CD8 T cells in hepatitis B infection: a flow cytometric analysis

2001 ◽  
Vol 124 (3) ◽  
pp. 435-444 ◽  
Author(s):  
S. Matsumura ◽  
K. Yamamoto ◽  
N. Shimada ◽  
N. Okano ◽  
R. Okamoto ◽  
...  
Keyword(s):  
T Cells ◽  
Hepatitis B ◽  
Cd8 T Cells ◽  
High Frequency ◽  
Flow Cytometric Analysis ◽  
Hepatitis B Infection ◽  
Flow Cytometric

289. Comparison of lymphocyte subsets and function in the rat and mouse testis

2005 ◽  
Vol 17 (9) ◽  
pp. 122 ◽  
Author(s):  
D. Aridi ◽  
D. Pellicci ◽  
P. Hutchinson ◽  
M. P. Hedger
Keyword(s):  
T Cells ◽  
Cell Line ◽  
Tumour Cell ◽  
Flow Cytometric Analysis ◽  
Nkt Cells ◽  
Mouse Testis ◽  
Tumour Cell Line ◽  
Rat Testis ◽  
Flow Cytometric ◽  
Rats And Mice

Testicular leukocytes are assumed to be involved in immunological surveillance against infection and tumours as well as regulation of local immune responses. They are implicated in mechanisms that make the testis a successful site for tissue transplantation in both rats and mice. Our previous studies using multi-colour fluorescence flow cytometric analysis to examine isolated testicular leukocytes in the rat testis have established the existence of a significant population of predominantly CD8+ T cells and a comparable number of lymphocytes expressing natural killer (NK) cell markers (NK and NKT cells). The functional activity of these testicular NK and NKT cells subsequently has been confirmed by a standard flow cytometric cytotoxicity assay using an NK-sensitive tumour cell line (YAC-1) and an NKT-sensitive tumour cell line (U937). Similar analyses of mouse testicular leukocytes have shown a slightly different pattern. The data indicate that mouse testicular lymphocytes comprise T cells, NK cells, and NKT cells, similar to the rat testis. However, while the apparent numerical densities of T cells in rat and mouse testes were similar, the numbers of NK and NKT cells were considerably lower in the mouse. Mouse testicular NKT cells were positive for staining with the tetramer CD1d/αGC, which is used to identify classical NKT cells, whereas rat NKT cells did not stain for this marker. Moreover, the CD8/CD4 T cell ratio in the mouse testis displayed a skewing towards the CD4+ subset. These data highlight the possibility that the immunological environment, and hence the course of immunological events, might be quite different in the testes of the two species. The reasons for these differences are not clear, however they should be taken into account when considering studies of testicular immune processes. Finally, comparative studies of immunological process in the testes of rats and mice may be very informative.

QUANTITATION OF IMMUNOSUPPRESSION BY FK506 USING FLOW CYTOMETRIC ANALYSIS OF IL-2 AND INF-γ INHIBITION IN PERIPHERAL BLOOD T CELLS.

2000 ◽  
Vol 69 (Supplement) ◽  
pp. S393 ◽  
Author(s):  
Mamun Ahmed ◽  
Raman Venkataraman ◽  
Alison J. Logar ◽  
Abdul S. Rao ◽  
Griffith P. Bartley ◽  
...  
Keyword(s):  
T Cells ◽  
Peripheral Blood ◽  
Flow Cytometric Analysis ◽  
Flow Cytometric

scholarly journals Arginase I Release from Activated Neutrophils Induces Peripheral Immunosuppression in a Murine Model of Stroke

2015 ◽  
Vol 35 (10) ◽  
pp. 1657-1663 ◽  
Author(s):  
Trisha R Sippel ◽  
Takeru Shimizu ◽  
Frank Strnad ◽  
Richard J Traystman ◽  
Paco S Herson ◽  
...  
Keyword(s):  
T Cells ◽  
Ischemic Stroke ◽  
T Cell ◽  
Murine Model ◽  
Cell Function ◽  
Culture Media ◽  
Flow Cytometric Analysis ◽  
Post Stroke ◽  
Activated Neutrophils ◽  
Arginase I

Transient suppression of peripheral immunity is a major source of complication for patients suffering from ischemic stroke. The release of Arginase I (ArgI) from activated neutrophils has recently been associated with T-cell dysfunction in a number of pathologies. However, this pathway has not been previously explored in ischemic stroke. Using the murine model of transient middle cerebral artery occlusion, we explored effects of stroke on peripheral T-cell function and evaluated the role of neutrophils and ArgI. Stimulation of splenic T cells from post-stroke animals with anti-CD3/CD28 resulted in decreased proliferation and interferon-γ production when compared with sham-surgery controls. Flow cytometric analysis of intrasplenic leukocytes exposed the presence of a transient population of activated neutrophils that correlated quantitatively with elevated ArgI levels in culture media. In vitro activation of purified resting neutrophils from unmanipulated controls confirmed the capacity for murine neutrophils to release ArgI from preformed granules. We observed decreased expression of the L-arg-sensitive CD3ζ on T cells, consistent with decreased functional activity. Critically, L-arg supplementation restored the functional response of post-stroke T cells to mitogenic stimulation. Together, these data outline a novel mechanism of reversible, neutrophil-mediated peripheral immunosuppression related to ArgI release following ischemic stroke.

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