scholarly journals International Standard Reagents for HPV Detection

2007 ◽  
Vol 23 (4) ◽  
pp. 283-296 ◽  
Author(s):  
Sonia R. Pagliusi ◽  
Suzanne M. Garland
Keyword(s):  
Disease Burden ◽  
Hpv Vaccination ◽  
Detection Methods ◽  
Analytical Sensitivity ◽  
International Standard ◽  
Laboratory Services ◽  
Hpv Types ◽  
Cellular Markers ◽  
Increasing Demand ◽  
The Impact

Humam papillomavirus is the commonest genital viral infection in healthy sexually active subjects, and the presence of chronic or persistent HPV types in genital cells may constitute a prognostic marker of underlying, or predict future HPV-associated diseases. A variety of novel tests for detecting the presence of oncogenic HPV types in biological specimens have been reported. These are based on the various stages of infection and viral life cycle. HPV infects squamous epithelium with expression of various gene products intimately linked to epithelial cell differentiation. Hence, there are basically three classes of detectable markers directly derived from HPVs: molecular markers based on detection of nucleic acid sequences, serological markers based on detection of antibodies against viral proteins, and cellular markers based on detection of proteins expressed intracellularly, upon either infection or carcinogenesis. The nature of various assays and the development of international standard reagents for qualitative and quantitative assessment of assay performance are outlined. There is an increasing demand to develop standard tools to assess the quality of HPV detection systems, for regulatory and clinical management purposes. International standard reagents for HPV will help defining the analytical sensitivity and specificity of various detection methods, and will allow assuring that laboratory services used to evaluate disease burden, HPV vaccines, and cancer prevention strategies are accurate and comparable worldwide. The advancement of prophylactic vaccine candidates against HPV infections and related diseases stresses the increasing importance of HPV assays in monitoring the impact of HPV vaccination on disease burden.

scholarly journals Seroprevalence of mucosal and cutaneous human papillomavirus (HPV) types among children and adolescents in the general population in Germany

2022 ◽  
Vol 22 (1) ◽  
Author(s):  
Anna Loenenbach ◽  
Michael Pawlita ◽  
Tim Waterboer ◽  
Thomas Harder ◽  
Christina Poethko-Müller ◽  
...  
Keyword(s):  
Children And Adolescents ◽  
Adult Population ◽  
Hpv Vaccination ◽  
Serological Response ◽  
Serum Samples ◽  
Representative Data ◽  
Hpv Types ◽  
Nationally Representative ◽  
The Impact ◽  
Hpv 1

Abstract Background In Germany, HPV vaccination of adolescent girls was introduced in 2007. Nationally representative data on the distribution of vaccine-relevant HPV types in the pre-vaccination era are, however, only available for the adult population. To obtain data in children and adolescents, we assessed the prevalence and determinants of serological response to 16 different HPV types in a representative sample of 12,257 boys and girls aged 1–17 years living in Germany in 2003–2005. Methods Serum samples were tested for antibodies to nine mucosal and seven cutaneous HPV types. The samples had been collected during the nationally representative German Health Interview and Examination Survey for Children and Adolescents in 2003–2006. We calculated age- and gender-specific HPV seroprevalence. We used multivariable regression models to identify associations between demographic and behavioral characteristics and HPV seropositivity. Results We found low but non-zero seroprevalence for the majority of tested HPV types among children and adolescents in Germany. The overall seroprevalence of HPV-16 was 2.6%, with slightly higher values in adolescents. Seroprevalence of all mucosal types but HPV-6 ranged from 0.6% for HPV-33, to 6.4% for HPV-31 and did not differ by gender. We found high overall seroprevalence for HPV-6 with 24.8%. Cutaneous HPV type seroprevalence ranged from 4.0% for HPV-38 to 31.7% for HPV-1. In the majority of cutaneous types, seroprevalence did not differ between boys and girls, but increased sharply with age, (e.g., HPV-1 from 1.5% in 1–3-years-old to 45.1% in 10–11-years-old). Associations between behavioral factors and type-specific HPV prevalence were determined to be heterogeneous. Conclusions We report the first nationally representative data of naturally acquired HPV antibody reactivity in the pre-HPV-vaccination era among children and adolescents living in Germany. These data can be used as baseline estimates for evaluating the impact of the current HPV vaccination strategy targeting 9–14-years-old boys and girls.

Estimating the prevalence of and treatment patterns for juvenile onset recurrent respiratory papillomatosis in Australia pre-vaccination: a pilot study

Sexual Health ◽  
2010 ◽  
Vol 7 (3) ◽  
pp. 253 ◽  
Author(s):  
Daniel Novakovic ◽  
Alan T. L. Cheng ◽  
Daron H. Cope ◽  
Julia M. L. Brotherton
Keyword(s):  
Disease Burden ◽  
Online Survey ◽  
Chart Review ◽  
Retrospective Chart Review ◽  
Tertiary Hospital ◽  
Recurrent Respiratory Papillomatosis ◽  
Juvenile Onset ◽  
Hpv Types ◽  
The Impact ◽  
Near Future

Background: Recurrent respiratory papillomatosis (RRP) causes serious morbidity. RRP in Australia may be eliminated in the near future following the implementation of a national vaccination program using a human papillomavirus (HPV) vaccine that protects against infection with HPV types 6 and 11, those responsible for RRP. Baseline data on RRP prevalence and disease burden in Australia are lacking. Methods: Three study methods were used to estimate the burden of juvenile onset RRP in Australia. We conducted a retrospective chart review of RRP cases treated at The Children’s Hospital at Westmead over 10 years, examined the coding of these cases, and then calculated and applied the positive predictive value of the codes to national data to estimate the prevalence of RRP in Australia. We also conducted an online survey of otolaryngologists in Australia who manage RRP. Results: Nineteen patients were treated at the hospital over 10 years, involving 359 admissions. We estimate that between 33 and 56 RRP cases aged <20 are being treated nationally per year (0.6–1.1 per 100 000 persons), with children 5–9 years having a higher estimated rate of 1.2–1.8 per 100 000. Among 39 otolaryngologists treating juvenile onset RRP, the majority (73%) treated RRP in a paediatric tertiary hospital, and used the microdebrider for ablation of lesions. Conclusions: Our estimates of RRP disease burden agree with international estimates. As a small number of clinicians treat RRP nationally, we believe that establishment of a national RRP register is both feasible and necessary to monitor the impact of vaccination.

scholarly journals The impact of 10 years of human papillomavirus (HPV) vaccination in Australia: what additional disease burden will a nonavalent vaccine prevent?

2018 ◽  
Vol 23 (41) ◽  
Author(s):  
Cyra Patel ◽  
Julia ML Brotherton ◽  
Alexis Pillsbury ◽  
Sanjay Jayasinghe ◽  
Basil Donovan ◽  
...  
Keyword(s):  
Vaccination Programme ◽  
Disease Burden ◽  
Hpv Vaccination ◽  
Genital Warts ◽  
Hpv Infection ◽  
High Coverage ◽  
Related Disease ◽  
Hpv Prevalence ◽  
Vaccine Type ◽  
The Impact

Background: A National human papilloma virus (HPV) Vaccination Programme for the prevention of HPV infection and associated disease using the quadrivalent HPV vaccine (4vHPV) has been funded and implemented in Australia since 2007, initially for girls only and extended to boys in 2013, with uptake rates among the highest observed worldwide. Aim: We report on the impact of this national programme on HPV prevalence and associated disease burden and estimate the potential impact of adopting a nonavalent HPV (9vHPV) vaccine. Methods: We performed a non-systematic literature review of studies measuring the burden of HPV-associated disease and infection in Australia before and after introduction of HPV vaccination. We also included key national reports with estimates of HPV-related disease burden. Results: Substantial declines in high-grade cervical disease and genital warts among vaccine-eligible women have been observed. Reductions in genital warts incidence and HPV prevalence among heterosexual men of similar age were observed before introduction of the male vaccination programme, indicating a substantial herd effect. 9vHPV vaccine is expected to prevent up to 90% of cervical and 96% of anal cancers. Of an estimated 1,544 HPV-associated cancers in 2012, 1,242 would have been preventable by the 4vHPV vaccine and an additional 187 anogenital cancers by the 9vHPV vaccine. Conclusions: Vaccination using 4vHPV vaccine has had a large demonstrable impact on HPV-related disease in Australia. A switch to 9vHPV could further reduce the HPV-associated cancer burden. With continued high coverage among both males and females, elimination of vaccine-type HPV disease seems achievable in Australia.

scholarly journals Impact and cost-effectiveness of nonavalent human papillomavirus vaccination in Switzerland: insights from a dynamic transmission model

2019 ◽  
Author(s):  
Maurane Riesen ◽  
Johannes A. Bogaards ◽  
Nicola Low ◽  
Christian L. Althaus
Keyword(s):  
Cervical Cancer ◽  
Sensitivity Analysis ◽  
Cost Effectiveness ◽  
Hpv Vaccination ◽  
Quadrivalent Vaccine ◽  
Transmission Model ◽  
Price Difference ◽  
Dynamic Transmission Model ◽  
Hpv Types ◽  
The Impact

ABSTRACTAIMIn Switzerland, human papillomavirus (HPV) vaccination has been implemented using a quadrivalent vaccine that covers HPV types 16 and 18, responsible for about 70% of cervical cancer. The average national uptake was 56% in girls by the age of 16 years in 2014–2016. A nonavalent vaccine, covering five additional oncogenic HPV types was recommended at the end of 2018. The primary aim of this study was to assess the impact and cost-effectiveness of introducing the nonavalent HPV vaccine in Switzerland compared with the quadrivalent vaccine.METHODSWe developed a dynamic transmission model that describes the spread of 10 high risk HPV types. We informed the model with Swiss data about sexual behaviour and cervical cancer screening, and calibrated the model to cervical cancer incidence in Switzerland. We modelled the impact of quadrivalent and nonavalent vaccines at the achieved (56%) and national recommended uptake (80%) in girls. We calculated the incremental cost-effectiveness ratio (ICER) between the nonavalent vaccine, the quadrivalent vaccine and no vaccination. We evaluated costs linked to cervical cancer screening, treatment of different disease stages and vaccination in a sensitivity analysis.RESULTSCompared with quadrivalent HPV vaccination in Switzerland at 56% uptake, vaccinating with the nonavalent vaccine would avert 1,175 cervical cancer deaths, 3,641 cases of cervical cancer and 106,898 CIN treatments over 100 years at 56% uptake. Compared with the quadrivalent vaccine, which would prevent an estimated 67% and 72% of cervical cancer cases at 56% and 80% coverage, the nonavalent vaccine would prevent 83% and 89% of all cervical cancers at the same coverage rates. The sensitivity analysis shows that introducing the nonavalent vaccination should improve health outcomes and offers a cost-saving alternative to the quadrivalent vaccine under the current price difference.CONCLUSIONSAll scenarios with quadrivalent and nonavalent vaccination are likely to be cost-effective compared with no vaccination. Switching to the nonavalent vaccine at current and improved vaccination uptake is likely to be cost-saving under the investigated price difference.

Universal Human Papillomavirus Vaccination and its Impact on the Southern Italian Region

2020 ◽  
Vol 26 (3) ◽  
pp. 343-357 ◽  
Author(s):  
Marcello Guido ◽  
Annarita Bruno ◽  
Luigi Tagliaferro ◽  
Valerio Aprile ◽  
Andrea Tinelli ◽  
...  
Keyword(s):  
Vaccination Coverage ◽  
Hpv Vaccination ◽  
Invasive Cervical Cancer ◽  
Hpv Infection ◽  
Vaccine Uptake ◽  
Local Health ◽  
Italian Region ◽  
Vaccine Type ◽  
Hpv Types ◽  
The Impact

HPV is still the most common sexually transmitted infection, leading to the onset of many disorders while causing an increase in direct and indirect health costs. High Risk (HR) HPV is the primary cause of invasive cervical cancer and contributes significantly to the development of anogenital and oropharyngeal cancers. The introduction of universal HPV vaccination has led to a significant reduction in vaccine-targeted HPV infections, cross-protective genotypes, precancerous lesions and anogenital warts. Despite the several limitations of HPV vaccination programs, including vaccine type specificity, different schedules, target age-groups and poor communication, the impact has become increasingly evident, especially in countries with high vaccine uptake. We carried out a review of the most recent literature to evaluate the effects of HPV vaccination on vaccinetargeted HPV genotypes and to assess the level of cross-protection provided against non-vaccine HPV types. Subsequently, to assess the rates of HPV infection in a southeast Italian region, we performed an epidemiological investigation on the impact of vaccination on genotypes and on the prevalence and distribution of HPV infection during the twelve-year period 2006-2017 in the Local Health Unit (LHU) of Lecce. The vaccination coverage of about 70% among girls in the LHU led to an initial reduction in vaccine-targeted HPV types and cross-protective genotypes. However, the results on this population should be interpreted cautiously because the period since the start of vaccination is too short and the coverage rate is not yet optimal to evaluate the efficacy of vaccination in lowering the prevalence of non-vaccine HR HPV types in the vaccinated cohort and in older subjects. Nevertheless, it is expected that direct effects will increase further and that herd immunity will begin to emerge as vaccination coverage increases.

scholarly journals NanoString Technology for Human Papillomavirus Typing

Viruses ◽  
2021 ◽  
Vol 13 (2) ◽  
pp. 188
Author(s):  
Mangalathu S. Rajeevan ◽  
Sonya Patel ◽  
Tengguo Li ◽  
Elizabeth R. Unger
Keyword(s):  
Limit Of Detection ◽  
Globin Gene ◽  
Hpv Vaccination ◽  
Specific Detection ◽  
Digital Readout ◽  
Hpv Types ◽  
Roche Linear Array ◽  
Nanostring Technology ◽  
The Impact ◽  
Single Reaction

High-throughput HPV typing assays with increased automation, faster turnaround and type-specific digital readout would facilitate studies monitoring the impact of HPV vaccination. We evaluated the NanoString nCounter® platform for detection and digital readout of 48 HPV types in a single reaction. NanoString (NS) used proprietary software to design CodeSets: type-specific probe pairs targeting 48 HPV types and the globin gene. We tested residual DNA extracts from epidemiologic specimens and defined samples (HPV plasmids at 10 to 104 copies/reaction) directly (No-PCR) as well as after L1 consensus PCR of 45 (PCR-45) or 15 cycles (PCR-15). Assay and interpretation followed NS recommendations. We evaluated analytic performance by comparing NanoString results for types included in prior assays: Roche Linear Array (LA) or HPV TypeSeq assay. No-PCR results on 40 samples showed good type-specific agreement with LA (k = 0.621) but sensitivity was 65% with lower limit of detection (LOD) at 104 plasmid copies. PCR-45 results showed almost perfect type-specific agreement with LA (k = 0.862), 82% sensitivity and LOD at 10 copies. PCR-15 results on 75 samples showed substantial type-specific agreement with LA (k = 0.796, 92% sensitivity) and TypeSeq (k = 0.777, 87% sensitivity), and LOD at 10 copies of plasmids. This proof-of-principle study demonstrates the efficacy of the NS platform with HPV CodeSet for type-specific detection using a low number of PCR cycles (PCR-15). Studies are in progress to evaluate assay reproducibility and analytic validation with a larger number of samples.

scholarly journals Assessment of the State of Knowledge about HPV Infection and HPV Vaccination among Polish Resident Doctors

2021 ◽  
Vol 18 (2) ◽  
pp. 551
Author(s):  
Katarzyna Smolarczyk ◽  
Wojciech Pieta ◽  
Slawomir Majewski
Keyword(s):  
Human Papillomavirus ◽  
Primary Prevention ◽  
Hpv Vaccine ◽  
Hpv Vaccination ◽  
Hpv Infection ◽  
The State ◽  
Vaccination Programs ◽  
Gynecology And Obstetrics ◽  
Hpv Types ◽  
The Impact

HPV (human papillomavirus) vaccinations have been introduced into the population of many countries through vaccination programs, although their acceptance varies from country to country, largely dependent on the state of knowledge about diseases caused by genital HPV types as well as cultural, social, and religious factors. The aim of the study was to analyze the state of knowledge about HPV and HPV vaccines among doctors during their specialization in gynecology and obstetrics, dermatology and venereology, and pediatrics. Another objective of the study was to analyze the impact of the state of knowledge about HPV vaccination on their attitude to primary prevention, i.e., vaccinations. A questionnaire was used to collect the data and 639 doctors took part in the study. The analysis was carried out mainly using descriptive statistical methods. In Poland, doctors’ knowledge about HPV is low, independent of gender, age, and subject of specialization. Doctors’ knowledge about the HPV vaccine is very low and independent of sex, age, and subject of specialization. However, doctors’ knowledge about HPV and the HPV vaccine influences the attitude to HPV vaccination and does not affect pro-active behaviors.

THE IMPACT OF UKRAINE'S EXCISE DUTY POLICY ON THE TRANSFORMATION PROCESSES OF THE TOBACCO MARKET

2020 ◽  
Vol 2020 (10) ◽  
pp. 19-33
Author(s):  
Nadiia NOVYTSKA ◽  
◽  
Inna KHLIEBNIKOVA ◽  
Keyword(s):  
Structural Changes ◽  
Tax Avoidance ◽  
Transformation Process ◽  
State Regulation ◽  
Tobacco Products ◽  
Excise Duty ◽  
Increasing Demand ◽  
High Level ◽  
The Impact ◽  
Transformation Processes

The market of tobacco products in Ukraine is one of the most dynamic and competitive. It develops under the influence of certain factors that cause structural changes, therefore, the aim of the article is to conduct a comprehensive analysis of transformation processes in the market of tobacco and their alternatives in Ukraine and identify the factors that cause them. The high level of tax burden and the proliferation of alternative products with a potentially lower risk to human health, including heating tobacco products and e-cigarettes, are key factors in the market’s transformation process. Their presence leads to an increase in illicit turnover of tobacco products, which accounts for 6.37% of the market, and the gradual replacement of cigarettes with alternative products, which account for 12.95%. The presence on the market of products that are not taxed or taxed at lower rates is one of the reasons for the reduction of excise duty revenues. According to the results of 2019, the planned indicators of revenues were not met by 23.5%. Other reasons for non-fulfillment of excise duty revenues include: declining dynamics of the tobacco products market; reduction in the number of smokers; reorientation of «cheap whites» cigarette flows from Ukraine to neighboring countries; tax avoidance. Prospects for further research are identified, namely the need to develop measures for state regulation and optimization of excise duty taxation of tobacco products and their alternatives, taking into account the risks to public health and increasing demand of illegal products.

scholarly journals Prostate cancer and the human papilloma virus: causative association, role of vaccines, and the impact of the COVID-19 pandemic

2021 ◽  
Author(s):  
Naomi Morka ◽  
Joseph M. Norris ◽  
Mark Emberton ◽  
Daniel Kelly
Keyword(s):  
Prostate Cancer ◽  
Human Papilloma Virus ◽  
Hpv Vaccination ◽  
Significant Proportion ◽  
Vaccine Uptake ◽  
Papilloma Virus ◽  
Vaccination Campaigns ◽  
The Impact ◽  
Hpv Vaccine Uptake

AbstractProstate cancer affects a significant proportion of men worldwide. Evidence from genetic and clinical studies suggests that there may be a causal association between prostate cancer and the human papilloma virus (HPV). As HPV is a vaccine-preventable pathogen, the possibility of a role in prostate cancer causation may reinforce the importance of effective HPV vaccination campaigns. This is of particular relevance in light of the COVID-19 pandemic, which may have considerable effects on HPV vaccine uptake and distribution.

scholarly journals A Systematic Review of Landsat Data for Change Detection Applications: 50 Years of Monitoring the Earth

2021 ◽  
Vol 13 (15) ◽  
pp. 2869
Author(s):  
MohammadAli Hemati ◽  
Mahdi Hasanlou ◽  
Masoud Mahdianpari ◽  
Fariba Mohammadimanesh
Keyword(s):  
Time Series ◽  
Change Detection ◽  
Policy Development ◽  
Atmospheric Correction ◽  
Open Data ◽  
Detection Methods ◽  
Landsat 8 ◽  
Landsat Images ◽  
Detection Analysis ◽  
The Impact

With uninterrupted space-based data collection since 1972, Landsat plays a key role in systematic monitoring of the Earth’s surface, enabled by an extensive and free, radiometrically consistent, global archive of imagery. Governments and international organizations rely on Landsat time series for monitoring and deriving a systematic understanding of the dynamics of the Earth’s surface at a spatial scale relevant to management, scientific inquiry, and policy development. In this study, we identify trends in Landsat-informed change detection studies by surveying 50 years of published applications, processing, and change detection methods. Specifically, a representative database was created resulting in 490 relevant journal articles derived from the Web of Science and Scopus. From these articles, we provide a review of recent developments, opportunities, and trends in Landsat change detection studies. The impact of the Landsat free and open data policy in 2008 is evident in the literature as a turning point in the number and nature of change detection studies. Based upon the search terms used and articles included, average number of Landsat images used in studies increased from 10 images before 2008 to 100,000 images in 2020. The 2008 opening of the Landsat archive resulted in a marked increase in the number of images used per study, typically providing the basis for the other trends in evidence. These key trends include an increase in automated processing, use of analysis-ready data (especially those with atmospheric correction), and use of cloud computing platforms, all over increasing large areas. The nature of change methods has evolved from representative bi-temporal pairs to time series of images capturing dynamics and trends, capable of revealing both gradual and abrupt changes. The result also revealed a greater use of nonparametric classifiers for Landsat change detection analysis. Landsat-9, to be launched in September 2021, in combination with the continued operation of Landsat-8 and integration with Sentinel-2, enhances opportunities for improved monitoring of change over increasingly larger areas with greater intra- and interannual frequency.

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