scholarly journals Application of a low-angle light scattering technique to cell volume and cell signaling studies on Ehrlich ascite tumor cells

2006 ◽  
Vol 20 (2) ◽  
pp. 45-55 ◽  
Author(s):  
Valeriy P. Zinchenko ◽  
Vyacheslav V. Lee ◽  
Alexey V. Berezhnov ◽  
Igor V. Mindukshev ◽  
Richard O. Jenkins ◽  
...  
Keyword(s):  
Light Scattering ◽  
Cell Signaling ◽  
Cell Volume ◽  
Tumor Cells ◽  
High Amplitude ◽  
Theoretical Background ◽  
Quantitative Measurements ◽  
Scattering Technique ◽  
Different Dimensions ◽  
Light Scattering Technique

A method for studying cells based on low-angle light scattering was applied to cell volume and cell signaling studies on Ehrlich ascite tumor cells (EATC). Changes in the volume of EATC were measured in hypotonic medium, as well as after activation with exogenous ATP, ionomycin and thimerosal. Increase of [Ca2+]iunder ATP and ionomycin action induced reversible changes of cell volume: fast shrinking was followed by swelling. Thimerosal caused a reversible change in EATC volume with high amplitude; endoplasmic reticulum played the key role in this response. Having obtained kinetic parameters of changes in cell volume under activation of the cells, quantitative measurements of K+, Na+and anion flows responsible for this process can then be obtained. In spite of some fundamental differences in the behavior of cells of different dimensions there are many similarities, and there is a good theoretical background for dealing with both small and large cells.

scholarly journals Osmotic water permeability of the human red cell. Dependence on direction of water flow and cell volume.

1983 ◽  
Vol 81 (2) ◽  
pp. 213-220 ◽  
Author(s):  
H J Mlekoday ◽  
R Moore ◽  
D G Levitt
Keyword(s):  
Light Scattering ◽  
Cell Volume ◽  
Half Time ◽  
Average Value ◽  
Osmotic Water ◽  
Medium Osmolarity ◽  
Scattering Technique ◽  
Scattering Signal ◽  
Cell Volumes ◽  
Light Scattering Technique

The osmotic permeability coefficient (Pf) was measured with a stopped-flow light-scattering technique. There is an artifactual light-scattering signal produced by the initial mixing that decays with a half-time of approximately 0.2 s. This seriously interferes with the measurement of the osmotically induced change in cell volume, which has a similar half-time. This "injection artifact" is associated with the biconcave shape of the cells. It is negligible for cells that have been made nearly spherical by swelling them in 160 mosmol. The dependence of this artifact on the cell volume may explain the previously observed dependence of Pf on the cell volume. When cells are made echinocytic (and therefore spherically symmetric), this injection artifact becomes negligible at all cell volumes and Pf can be accurately measured. The Pf of echinocytic cells was nearly constant, varying by less than 10% with the direction of flow and the medium osmolarity (160-360 mosmol). The average value of Pf was 2.0 X 10(-2) cm/s (T = 23 degrees C).

An assay of DNA by resonance light scattering technique and its application in screening anticancer drugs

2012 ◽  
Vol 4 (6) ◽  
pp. 1546-1551 ◽  
Author(s):  
Xi Chen ◽  
Guoliang Liu ◽  
Shengwang Liang ◽  
Sihua Qian ◽  
Jinbin Liu ◽  
...  
Keyword(s):  
Light Scattering ◽  
Anticancer Drugs ◽  
Resonance Light Scattering ◽  
Scattering Technique ◽  
Resonance Light Scattering Technique ◽  
Light Scattering Technique
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