scholarly journals Human Gastric Mucosal Hydrophobicity Does dot Decrease with Helicobacter Pylori Infection or Chronological Age

2005 ◽  
Vol 19 (1) ◽  
pp. 37-41
Author(s):  
Mohammed S Al-Marhoon ◽  
Sheila Nunn ◽  
Roger W Soames
Keyword(s):  
Contact Angle ◽  
Helicobacter Pylori ◽  
Computer Software ◽  
Contact Angles ◽  
Chronological Age ◽  
Peptic Ulcers ◽  
Gastric Mucus ◽  
Gastric Diseases ◽  
Significant Difference ◽  
H Pylori

BACKGROUND AND AIMS: Infection with cytotoxin-associated gene A (cagA) Helicobacter pylori is associated with severe gastric diseases. Previous studies in humans have reported a decreased gastric hydrophobicity with H pylori infection. The aim of the present study was to differentiate between the effect of cagA+ and cagA- strains on gastric mucus hydrophobicity.METHODS: One hundred patients without peptic ulcers and not on medication were randomly recruited from endoscopy clinics; each patient had six biopsies. Contact angle measurements were performed using a goniometer assisted by computer software. H pylori status was assessed by histology, Campylobacter-like organism test and culture, and cagA+ status was determined by polymerase chain reaction.RESULTS: In age- and sex-matched patients, there was no significant difference (P=0.27) in contact angle between H pylori-positive (61±2.8°) and H pylori-negative patients (65.5±3.0°). There was also no significant difference (P=0.36) in contact angle among H pylori-negative, cagA- and cagA+ patients (65.5±3.0°, 58.6±3.6° and 63.4±4.9°, respectively). However, a trend of increased mean contact angles in cagA+ compared with cagA- and H pylori-negative patients was observed in patients 50 years and younger (68.3±8.3°, 61.1±6.1° and 63.6±2.2°, respectively; P=0.70) and in patients without atrophy (71.1±8°, 59.6±4° and 66±2°, respectively; P=0.30). In addition, there was no significant correlation between contact angles and patient age (r=0.104, P=0.306).CONCLUSIONS: The present study shows that H pylori infection and the chronological age have no effect on the gastric mucus hydrophobicity, but it highlights a trend of increased mucus hydrophobicity with cagA+ infection that needs to be supported by future studies.

scholarly journals Cross-sectional study of human coding- and non-coding RNAs in progressive stages of Helicobacter pylori infection

2020 ◽  
Vol 7 (1) ◽  
Author(s):  
Sergio Lario ◽  
María J. Ramírez-Lázaro ◽  
Aintzane González-Lahera ◽  
José L. Lavín ◽  
Maria Vila-Casadesús ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Helicobacter Pylori ◽  
Expression Profiles ◽  
Gastric Carcinogenesis ◽  
Individual Response ◽  
Peptic Ulcers ◽  
Gastric Diseases ◽  
Sequence Of Events ◽  
Non Coding Rnas ◽  
H Pylori

Abstract Helicobacter pylori infects 4.4 billion individuals worldwide and is considered the most important etiologic agent for peptic ulcers and gastric cancer. Individual response to H. pylori infection is complex and depends on complex interactions between host and environmental factors. The pathway towards gastric cancer is a sequence of events known as Correa’s model of gastric carcinogenesis, a stepwise inflammatory process from normal mucosa to chronic-active gastritis, atrophy, metaplasia and gastric adenocarcinoma. This study examines gastric clinical specimens representing different steps of the Correa pathway with the aim of identifying the expression profiles of coding- and non-coding RNAs that may have a role in Correa’s model of gastric carcinogenesis. We screened for differentially expressed genes in gastric biopsies by employing RNAseq, microarrays and qRT-PCR. Here we provide a detailed description of the experiments, methods and results generated. The datasets may help other scientists and clinicians to find new clues to the pathogenesis of H. pylori and the mechanisms of progression of the infection to more severe gastric diseases. Data is available via ArrayExpress.

A Study of the Relationship between Helicobacter pylori and Recurrent Aphthous Stomatitis Using a Urea Breath Test

2009 ◽  
Vol 10 (1) ◽  
pp. 9-16 ◽  
Author(s):  
Maryam Baharvand ◽  
Ziba Maleki ◽  
Kaveh Alavi ◽  
Ali Akbar Sayyari ◽  
Leila Sayyari
Keyword(s):  
Helicobacter Pylori ◽  
Breath Test ◽  
Urea Breath Test ◽  
Recurrent Aphthous Stomatitis ◽  
Aphthous Stomatitis ◽  
Control Group ◽  
Peptic Ulcers ◽  
Significant Difference ◽  
H Pylori ◽  
The Relationship

Abstract Aim Helicobacter pylori (H. pylori) is one of the most common, well-known pathogenic agents in the development of peptic ulcers. Some investigators have shown a relationship between H. pylori and recurrent aphthous stomatitis (RAS). However, this relationship is controversial. The aim of this study was to determine the association between H. pylori and RAS using the urea breath test (UBT). Methods and Materials Forty-three patients with RAS and 44 non-RAS controls were evaluated. There were no differences in gender or age in the two groups. The UBT was used to detect H. pylori infection. Data were analyzed using the Chi Square Test. Results Sixteen individuals in the RAS patients (37.2%) and 14 individuals in the control group (31.8%) had a positive breath test. The difference was not considered statistically significant (p=0.597). Conclusion In the present study no statistically significant difference was found between frequency of a positive UBT in the RAS patients and the control group. Clinical Significance Since the probability of a positive test was higher in the more severe cases this factor needs to be considered in the diagnosis and treatment of RAS. Citation Maleki Z, Sayyari AA, Alvavi K, Sayyari L, Baharvand M. A Study on the Relationship between Helicobacter pylori and Recurrent Aphthous Stomatitis Using a Urea Breath Test. J Contemp Dent Pract 2009 January; (10)1:009-016.

scholarly journals Galectin-2 Has Bactericidal Effects against Helicobacter pylori in a β-galactoside-Dependent Manner

2020 ◽  
Vol 21 (8) ◽  
pp. 2697 ◽  
Author(s):  
Takaharu Sasaki ◽  
Rei Saito ◽  
Midori Oyama ◽  
Tomoharu Takeuchi ◽  
Toru Tanaka ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Bactericidal Effect ◽  
Dependent Manner ◽  
Peptic Ulcers ◽  
Gastric Tissue ◽  
Gastric Mucus ◽  
Biological Phenomena ◽  
Bactericidal Effects ◽  
H Pylori

Helicobacter pylori is associated with the onset of gastritis, peptic ulcers, and gastric cancer. Galectins are a family of β-galactoside-binding proteins involved in diverse biological phenomena. Galectin-2 (Gal-2), a member of the galectin family, is predominantly expressed in the gastrointestinal tract. Although some galectin family proteins are involved in immunoreaction, the role of Gal-2 against H. pylori infection remains unclear. In this study, the effects of Gal-2 on H. pylori morphology and survival were examined. Gal-2 induced H. pylori aggregation depending on β-galactoside and demonstrated a bactericidal effect. Immunohistochemical staining of the gastric tissue indicated that Gal-2 existed in the gastric mucus, as well as mucosa. These results suggested that Gal-2 plays a role in innate immunity against H. pylori infection in gastric mucus.

scholarly journals Helicobacter pylori CagA Induces AGS Cell Elongation through a Cell Retraction Defect That Is Independent of Cdc42, Rac1, and Arp2/3

2006 ◽  
Vol 75 (3) ◽  
pp. 1203-1213 ◽  
Author(s):  
Kevin M. Bourzac ◽  
Crystal M. Botham ◽  
Karen Guillemin
Keyword(s):  
Helicobacter Pylori ◽  
Cell Elongation ◽  
Severe Disease ◽  
Significant Risk ◽  
Immunohistochemical Analysis ◽  
Significant Risk Factor ◽  
Host Cells ◽  
Peptic Ulcers ◽  
Gastric Diseases ◽  
H Pylori

ABSTRACT Helicobacter pylori, which infects over one-half the world's population, is a significant risk factor in a spectrum of gastric diseases, including peptic ulcers and gastric cancer. Strains of H. pylori that deliver the effector molecule CagA into host cells via a type IV secretion system are associated with more severe disease outcomes. In a tissue culture model of infection, CagA delivery results in a dramatic cellular elongation referred to as the “hummingbird” phenotype, which is characterized by long, thin cellular extensions. These actin-based cytoskeletal rearrangements are reminiscent of structures that are regulated by Rho GTPases and the Arp2/3 complex. We tested whether these signaling pathways were important in the H. pylori-induced cell elongation phenotype. Contrary to our expectations, we found that these molecules are dispensable for cell elongation. Instead, time-lapse video microscopy revealed that cells infected by cagA + H. pylori become elongated because they fail to release their back ends during cell locomotion. Consistent with a model in which CagA causes cell elongation by inhibiting the disassembly of adhesive cell contacts at migrating cells' lagging ends, immunohistochemical analysis revealed that focal adhesion complexes persist at the distal tips of elongated cell projections. Thus, our data implicate a set of signaling molecules in the hummingbird phenotype that are different than the molecules previously suspected.

scholarly journals Effects of Infections of Helicobacter Pylori With Different Virulent Factors on Severity of Gastrointestinal Diseases

2021 ◽  
Author(s):  
Yan Zhang ◽  
Xiang-ming Fang ◽  
Kui Tian
Keyword(s):  
Helicobacter Pylori ◽  
Bacterial Infections ◽  
Gastrointestinal Diseases ◽  
Ulcer Bleeding ◽  
Type I ◽  
Peptic Ulcers ◽  
Type Ii ◽  
Type Iii ◽  
Significant Difference ◽  
H Pylori

Abstract Background: Helicobacter Pylori (H. pylori) infection, one of the most common chronic bacterial infections, has been considered as a major cause of diseases such as lymphoma, gastritis, peptic ulcers, and stomach cancer. Here, we aimed to determine whether H. pylori strains with different virulence contribute to the gastrointestinal diseases differentially in clinical settings, which may provide future direction for eradication of H. pylori infection. Methods: We recruited 501 patients with gastrointestinal disorders for analysis of antibody types of H. pylori infection. Correlation analysis was done to determine the association of different virulence of H. pylori with patients’ baseline parameters and personal disease history. Next, subjects with each type of anti- H. pylori infection antibody were subjected to esophagogastro duodenoscopy（EGD) and colonoscopy examinations. The pathological diagnosis was also conducted in endoscopic samples. Chi-squared test was employed to compare the differences in endoscopic assessments and pathological findings among three types of H. pylori infection determined by the presence of antibodies to virulent factors. Results: There were 296 cases with Type I H. pylori infection, 120 cases with Type II H. pylori infection, and 85 cases without H. pylori infection (negative, Type III). No correlation was found between different virulence of H. pylori and participants’ baseline data (P > 0.05). EGD results showed that the incidences of peptic ulcer, bleeding and malignant lesions in Type I group were significantly higher than that in Type II and Type III (P＜0.05). Despite of increased trends of incidences of precancerous alterations and the malignance in Type I group compared with type II and III groups, there was no significant difference (P > 0.05). In addition, coloscopic features were similar among three groups. On the other hand, infections of H. pylori with cytotoxin-associated gene A (CagA) and/or vacuolating cytotoxin A (VacA) virulent factors resulted in more severe histopathological diseases than that with only Ure A/B factor and without infection (P < 0.05). Conclusions: Infections of H. pylori strains with CagA/VacA are likely to cause development of severe gastrointestinal diseases. These results are helpful to treat for H. pylori infection clinically.

scholarly journals The role of Helicobacter pylori in preeclampsia and in gastric diseases in pregnant women

2020 ◽  
Vol 7 (7) ◽  
pp. 2097
Author(s):  
Jehan Sabah Hasan ◽  
Mohammed Ahmed Alshami
Keyword(s):  
Helicobacter Pylori ◽  
Pregnant Women ◽  
Epigastric Pain ◽  
Peptic Ulcers ◽  
Gastric Diseases ◽  
Number Of Patients ◽  
Mild Preeclampsia ◽  
Laboratory Investigations ◽  
H Pylori

Background: Helicobacter pylori is a gastric organism was first observed more than 100 years ago. It may cause chronic gastritis, peptic ulcers and gastric adenocarcinoma and lymphoma. It can produce some extragastric disorders including preeclampsia. This study aimed to focus on the importance of H. pylori and its relationship with preeclampsia and gastric illnesses in pregnant patients.Methods: This study includeed 100 pregnant women, half of them were healthy and the other half with preeclampsia. Patients with chronic medical illnesses were excluded. Data was collected and laboratory investigations were done including that for H. pylori. Oesophagogastroduodenoscopy then was done 6 weeks post-delivery or termination of pregnancy for symptomatic H. pylori positive women.Results: 50% of the total number were healthy, 17% had mild preeclampsia, 33% suffering from severe preeclampsia. Seropositivity for H. pylori is significantly higher in preeclampsia and significantly related to severity of preeclampsia and complications of pregnancy. Positive H. pylori test in stool was seen in (45%) of total number of patients. Epigastric pain persist in 41 (91%) of patients with positive H. pylori test in stool. Gastritis, duodenal ulcer, and gastric ulcer were found in 85.3%, 12.1% and 2.4% of symptomatic patients respectively.Conclusions: Significant correlation between Helicobacter pylori seropositivity and preeclampsia may indicate the benefit of using this parameter in the prediction and management of preeclampsia and its severity. H. pylori infection plays an important role in gastric pathologies in pregnant women that can be dealt with or may be prevented more efficiently in future. 

scholarly journals Helicobacter pylori Infection in Tripoli, North Lebanon: Assessment and Risk Factors

Biology ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 599
Author(s):  
Ghalia Khoder ◽  
Sara Mina ◽  
Ibrahim Mahmoud ◽  
Jibran Sualeh Muhammad ◽  
Rania Harati ◽  
...  
Keyword(s):  
Risk Factors ◽  
Helicobacter Pylori ◽  
Demographic Data ◽  
Cross Sectional Study ◽  
Statistical Correlation ◽  
Binary Logistic Regression Analysis ◽  
Peptic Ulcers ◽  
Gastric Diseases ◽  
Cross Sectional ◽  
H Pylori

Helicobacter pylori (H. pylori) infection occurs among half of the general population worldwide, with high geographic variability. Even though H. pylori is the leading cause of several gastric diseases, ranging from gastritis and peptic ulcers to gastric malignancies, such as gastric cancer and mucosa-associated lymphoid tissue lymphoma, most of the infections remain asymptomatic. Early detection and eradication of H. pylori can definitely prevent severe long-term gastric diseases associated with H. pylori. In Lebanon, the prevalence of H. pylori is not well documented, especially in healthy subjects. The aim of this study is to assess H. pylori infections and the associated risk factors in Tripoli, North Lebanon. A cross-sectional study was conducted on 300 healthy Lebanese volunteers, including both children and adults. The H. pylori stool antigens were detected using the Premier Platinum HpSA test. The socio-demographic data, lifestyle characteristics, and gastrointestinal characteristics of all participants were analyzed. Out of the 300 tested volunteer subjects, 31% were found to be positive for H. pylori. A multivariate binary logistic regression analysis for factors associated with H. pylori infection revealed a significant association between H. pylori infection and gastrointestinal disturbances, the crowding index, and occupation. A significant statistical correlation was found between sheesha smoking (p = 0.001) and H. pylori infection. These findings highlight the need for the development of preventive approaches and strategic indications for the appropriate treatment of H. pylori infections in Tripoli, North Lebanon.

Increased Production of Matrix Metalloproteinases in Helicobacter pylori Infection that Stimulates Gastric Cancer Stem Cells

2020 ◽  
Keyword(s):  
Gastric Cancer ◽  
Stem Cells ◽  
Helicobacter Pylori ◽  
Cancer Stem Cells ◽  
Histopathological Examination ◽  
Physiological Saline ◽  
Rrna Gene ◽  
Peptic Ulcers ◽  
Gastric Cancer Stem Cells ◽  
H Pylori

Background and aim: Helicobacter pylori (H. pylori) is an incriminated pathogen causing diseases in both animals and humans and considered a zoonotic pathogen. H. pylori infection is considered a cause of gastric cancer, which rests a significant health care challenge. This study analyzes the expression pattern of matrix metalloprotein 2 (MMP-2) in patients with Helicobacter pylori-associated gastritis and the effect of H. pylori on gastric cancer stem cells, as well as study the role of helicon bacteriosis in dog in transmission of H. pylori infection to human. Materials and methods: Fifty-five of each sample (gastric biopsy, blood and stool) were collected from patients suffering from dyspepsia, chronic vomiting and perforated peptic ulcers and also from apparent healthy dogs. The investigation detected H. pylori by serological and histopathological examination. Biopsies were stored in physiological saline for identification of H. pylori by conventional time PCR. MMP-2 and Gastric cancer stem cells were then identified by immunohistochemistry. Results: Serological identification for H. pylori Antigen and Antibodies revealed (63% human, 50% dogs) and (87% human, 90% dogs) respectively were positive. Genotyping of H. pylori based on 16S rRNA gene showed 54.5% of human and 35% of dogs were positive. Immunohistochemistry revealed strong expression of CD44 in H. pylori- associated gastric cancer cases, MMP-2 expression was observed in all neoplastic lesions associated with H. pylori infection. Conclusion: H. pylori infection affects gastric mucosa and induces changes in gastric stem cells altering their differentiation and increased expression of MMP’s and CD44with a resultant potentiation of oncogenic alteration. In addition the up-regulation of both markers could be an instrumental to interpret the origination of gastric cancer.

Analysis of babA, cagE and cagA Genes in Helicobacter pylori from Upper Gastric Patients in the North of Iran

2019 ◽  
Vol 19 (3) ◽  
pp. 274-278 ◽  
Author(s):  
Saba Fakhrieh Asl ◽  
Mehrnaz Pourvahedi ◽  
Ali Mojtahedi ◽  
Mohammad Shenagari
Keyword(s):  
Helicobacter Pylori ◽  
Gastric Diseases ◽  
Specific Primers ◽  
The North ◽  
Different Types ◽  
North Of Iran ◽  
Gastric Biopsies ◽  
Pcr Method ◽  
Non Ulcer Dyspepsia ◽  
H Pylori

Objective:Helicobacter pylori is a Gram-negative bacterium which has a serious effect on up to half of the world’s population and has been related to different gastric diseases. The goal of this study was to assess the frequency of babA, cagE and cagA genotypes among H. pylori strains isolated from gastric biopsies of endoscopic patients in the north of Iran.Methods:The present study was performed on 90 strains of H. pylori isolated from patients with gastric diseases (Gastric ulcer (GU), Duodenal ulcer (DU), Gastritis (G), Non-ulcer dyspepsia (NUD) and Gastric adenocarcinoma (GC)). DNA was extracted from all isolated strains and PCR method was performed to detect the prevalence of babA2, cagE and cagA genes using specific primers.Results:Among 90 samples of H. pylori, babA2, cagE, and cagA genes were detected in 42.2%, 30% and 82.2% of strains respectively. The statistical analysis showed that the prevalence of cagA gene in GU, G, DU, and NUD was significantly higher than other genes. Moreover, cagA, and babA2 genes were significantly more prevalent in GC patients compared to cagE gene. Our isolates exhibited 8 distinct arrangements of virulence patterns. The occurrence of cagA (35.6%) was the most prevalent pattern followed by cagA/babA2 (20%) and cagA/babA2/cagE (14.4%).Conclusion:In summary, as first report from Guilan province in the north of Iran, we showed significant association between the presence of babA2, cagE, and cagA genes in different types of gastric disorders.

Perfil Epidemiológico de Pacientes Submetidos à Biópsia Gástrica em um Hospital Escola do Sul de Minas Gerais/ Epidemiological Profile of Patients Undergo Gastric Biopsy in a School Hospital of Minas Gerais

1970 ◽  
Vol 4 (3) ◽  
pp. 48-57
Author(s):  
Isabela Maria A. Ribeiro Simões ◽  
Ana Carolina Mauad Coli ◽  
Roseane de Souza Candido Irulegui
Keyword(s):  
Helicobacter Pylori ◽  
Age Groups ◽  
Epidemiological Data ◽  
Minas Gerais ◽  
Gastric Biopsy ◽  
Gastric Ulcers ◽  
Histopathological Diagnosis ◽  
Gastric Diseases ◽  
H Pylori ◽  
Epidemiological Profile

Objetivo: Determinar a prevalência de lesões benignas e neoplasia gástrica através do estudo de biópsias realizadas em um Hospital Escola do Sul de Minas Gerais, no período entre 2007 e 2011. Materiais e Métodos: A pesquisa documental foi quantitativa e retrospectiva, baseada na análise dos registros de biópsias e prontuários. Realizou-se o levantamento de dados referentes à idade, gênero, cor, profissão, diagnóstico histopatológico e presença de Helicobacter pylori nas amostras. Resultados: O número total de biópsias gástricas analisadas foi de 1225, cujo perfil populacional encontrado foi: idade média de 56,75 anos, sexo masculino (52%), cor branca (81,9 %), aposentado (30%). Os diagnósticos mais frequentes foram: gastrites (71,9%), pólipos (14,2%), adenocarcinomas (5,9%), úlceras gástricas (6%), linfomas (0,4%), sem alterações (0,4%) e outros (1,2%). Em outros, encontram-se achados de malignidade, metaplasia e xantelasma gástrico. Em relação à presença de Helicobacter pylori nas amostras, o resultado encontrado foi de24% positivas, 46% negativas e 30% não pesquisadas. Conclusão: Os resultados confirmam a alta frequência das doenças gástricas e sua incidência nas diversas faixas etárias, além do envolvimento do H. pylori em tais afecções. É de grande importância a caracterização dos dados epidemiológicos, o que permite prováveis direcionamentos para programas de prevenção e informação para a população. Palavras-chave: biópsia gástrica, gastropatia, perfil epidemiológico.  ABSTRACTObjective: To determine the prevalence of benign lesions and gastric cancer through study of biopsies performed at a school hospital in southern Minas Gerais, in the period between 2007 and 2011.Materials and Methods: The research was quantitative and retrospective, based on analysis of biopsies records and medical records. We conducted the survey data regarding age, sex, color, profession, histopathological diagnosis and the presence of Helicobacter pylori in the samples. Results: The total number of gastric biopsies analyzed was 1225. Population listing was found: mean age of 56.75 years, male (52%), white (81.9%), retired (30%). The most frequent diagnoses were gastritis (71.9%), polyps (14.2%), adenocarcinomas (5.9%), gastric ulcers (6%), lymphoma (0.4%), unchanged (0, 4%) and others (1.2%). In others, there are: findings of malignancy, metaplasia, gastric xanthelasma. Regarding the presence of Helicobacter pylori in the sample, the result was: 24% positive, 46% negative, 30% non searched. Conclusion: The results confirm the high frequency of gastric diseases and their incidence in the various age groups additionally to the involvement of H. pylori in such conditions. It is of great importance to characterize the epidemiological data, allowing probable directions for prevention and information programs for population. Keywords: gastric biopsy, gastropathy, epidemiological profile

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