scholarly journals The Laboratory Diagnosis of Bacterial Vaginosis

2005 ◽  
Vol 16 (2) ◽  
pp. 77-79 ◽  
Author(s):  
Deborah Money
Keyword(s):  
Bacterial Vaginosis ◽  
Pregnancy Outcomes ◽  
Laboratory Diagnosis ◽  
Vaginal Flora ◽  
Gram Stain ◽  
Transmitted Infection ◽  
Sexually Transmitted ◽  
Inappropriate Use ◽  
Future Developments ◽  
Stain Analysis

Bacterial vaginosis (BV) is an extremely common health problem for women. In addition to the troublesome symptoms often associated with a disruption in the balance of vaginal flora, BV is associated with adverse gynecological and pregnancy outcomes. Although not technically a sexually transmitted infection, BV is a sexually associated condition. Diagnostic tests include real-time clinical/microbiological diagnosis, and the current gold standard, the standardized evaluation of morphotypes on Gram stain analysis. The inappropriate use of vaginal culture can be misleading. Future developments into molecular-based diagnostics will be important to further understand this complex endogenous flora disruption.

scholarly journals Bacterial Vaginosis in Pregnant Women at Maternal and Child Hospital, West Java, Indonesia, 2018: High Prevalence in Asymptomatic Females

2019 ◽  
Vol 11 (2) ◽  
pp. 53-59
Author(s):  
Pati Aji Achdiat ◽  
Rasmia Rowawi ◽  
Irma Fakhrosa ◽  
Hendra Gunawan ◽  
Reti Hindritiani ◽  
...  
Keyword(s):  
Bacterial Vaginosis ◽  
Pregnant Women ◽  
Genital Tract Infection ◽  
West Java ◽  
Transmitted Infection ◽  
Cross Sectional ◽  
Sexually Transmitted ◽  
Human Immuno Deficiency Virus ◽  
Lower Genital Tract Infection ◽  
Child Hospital

Abstract Bacterial vaginosis (BV) is a lower genital tract infection of reproductive women which can occur in pregnant and non-pregnant women. BV in pregnant women can increase the risk of complications, including increased incidence of abortion, premature rupture of membranes, preterm birth, and babies with low birth weight. BV can also increase the risk of acquired sexually transmitted infection (STI) and their further transmission, including human immuno-deficiency virus (HIV). Each country has a different prevalence of BV. The previous report of BV prevalence in pregnant women was submitted in Jakarta, Indonesia in 1990. Until now, there is no update data of BV in pregnant women, especially in West Java, Indonesia. Thus, we conducted a descriptive observational study using a cross-sectional design and a consecutive sampling method in June 2018. This study included 60 pregnant women in the Maternal and Child Hospital, Bandung, Indonesia. Out of 60 participants, seven (11.67%) participants had BV according to Amsel criteria. Asymptomatic BV was diagnosed in all participants. This study shows the prevalence of BV in pregnant women in the Maternal and Child Hospital in Bandung during June 2018. The assessment of screening BV should be recommended as a routine workup. To avoid complications in pregnant women and infants it should not be waited for the symptoms to reveal.

scholarly journals The Laboratory Diagnosis ofHaemophilus ducreyi

2005 ◽  
Vol 16 (1) ◽  
pp. 31-34 ◽  
Author(s):  
Michelle Alfa
Keyword(s):  
Diagnostic Testing ◽  
Laboratory Diagnosis ◽  
Primary Method ◽  
Sample Collection ◽  
Culture Methods ◽  
Transmitted Infection ◽  
Diagnostic Laboratory ◽  
Sexually Transmitted ◽  
Alternative Approaches ◽  
Genetic Amplification

Chancroid is a sexually transmitted infection caused byHaemophilus ducreyi. This fastidious, Gram-negative coccobacilli dies rapidly outside the human host, making diagnostic testing using culture methods difficult. This genital ulcer infection is not common in Canada and, therefore, can often be misdiagnosed. The objective of the present paper is to provide practical approaches for the diagnosis of chancroid in Canadian patients where the prevalence of this infection is low. Issues related to sample collection, sample transport and available diagnostic tests are reviewed, and several alternative approaches are outlined. Although antigen detection, serology and genetic amplification methods have all been reported forH ducreyi, none are commercially available. Culture is still the primary method available to most laboratories. However, the special media necessary for direct bedside inoculation is often not available; therefore, communication with the diagnostic laboratory and rapid specimen transport are essential when chancroid is suspected

scholarly journals Pilot Study to Evaluate the Appropriate Management of Patients With Coexistent Bacterial Vaginosis and Cervicitis

1995 ◽  
Vol 3 (3) ◽  
pp. 119-122 ◽  
Author(s):  
Jane R. Schwebke ◽  
Mary Beth Schulien ◽  
Mark Zajackowski
Keyword(s):  
Targeted Therapy ◽  
Pilot Study ◽  
Phase I ◽  
Bacterial Vaginosis ◽  
Phase Ii ◽  
Preliminary Data ◽  
Sexually Transmitted Disease ◽  
Transmitted Disease ◽  
Vaginal Flora ◽  
Sexually Transmitted

A pilot study was performed to obtain preliminary data regarding the need for targeted therapy of bacterial vaginosis (BV) when it accompanies clinically defined cervicitis. Specifically, women attending a sexually transmitted disease (STD) clinic with clinically defined BV and cervicitis were treated in the first phase of the study with doxycycline alone. In phase II, the patients received doxycycline and concomitant intravaginal metronidazole gel. All patients were reexamined 3–4 weeks after therapy. Persistence of BV occurred in 18/19 (95%) of women in phase I vs. 1/7 (14%) of women in phase II (P < 0.001). We concluded that, in women with coexistent clinically defined cervicitis and BV, the treatment of cervicitis does not result in a normalization of the vaginal flora.

scholarly journals Availability of laboratory diagnosis of gonorrhoea and its meaning in case reporting in Shandong province, China

2020 ◽  
Author(s):  
Xinlong Chen ◽  
Yanling Gan ◽  
dianchang liu
Keyword(s):  
Laboratory Tests ◽  
Descriptive Analysis ◽  
Laboratory Diagnosis ◽  
Shandong Province ◽  
Clinical Services ◽  
Chi Square ◽  
Transmitted Infection ◽  
Sexually Transmitted ◽  
Significant Difference ◽  
Different Levels

Abstract Background The aim of this study is to understand the availability of laboratory diagnosis of gonorrhoea in Shandong province and its meaning in case reporting. Methods Two surveys were conducted among hospitals providing clinical services for sexually transmitted infection(STI) in Shandong Province in 2012 and 2018. The availability of laboratory tests for Neisseria gonorrhoeae (NG) and distribution of reported gonorrhea cases were compared among different hospitals provided clinical services for STI in Shandong province. Descriptive analysis and Chi-square were used for statistical analysis. Results Smear, culture and PCR tests for NG were used among 301(74.69%), 123(30.52%), 43(10.67%) hospitals in 2012 and 356(74.48%), 176(36.82%) and 73(15.27%) hospitals in 2018, respectively. Significant differences were observed in the availability of smear among hospitals at different levels (X2 = 12.159, p = 0.000) and with different affiliations(X2 = 4.551, p = 0.033) in 2018. Significant differences were observed in the availability of culture and PCR tests among hospitals at different levels both in 2012 (X2 = 34.532, p = 0.000; X2 = 7.380, p = 0.007) and 2018 (X2 = 49.820, p = 0.000; X2 = 21.658, p = 0.000). The availability of culture and PCR tests for NG among hospitals in 2018 was higher than that in 2012 (X2 = 3.870, p = 0.049; X2 = 4.050, p = 0.044). More hospitals reported gonorrhea cases in 2018 than in 2012 (X2 = 6.768, p = 0.009). A significant difference was observed in distribution of case reporting among hospitals at different levels in 2018 (X2 = 6.975, p = 0.008) and among different types of hospitals both in 2012 (X2 = 52.362, p = 0.000) and 2018 (X2 = 74.478, p = 0.000). Conclusion Poor availability of NG laboratory tests affects case reporting and is a crucial reason leading to high sex ratio and underreporting of gonorrhea cases in Shandong province. Trial registration Not applicable

scholarly journals Bacterial Vaginosis and Sexually Transmitted Infections in an HIV-Positive Cohort

2021 ◽  
Vol 3 ◽  
Author(s):  
Karina Nava-Memije ◽  
Cecilia Hernández-Cortez ◽  
Verónica Ruiz-González ◽  
Claudia A. Saldaña-Juárez ◽  
Yazmín Medina-Islas ◽  
...  
Keyword(s):  
Bacterial Vaginosis ◽  
Hiv And Aids ◽  
World Health ◽  
Hiv Patients ◽  
Transmitted Infection ◽  
Genital Infections ◽  
Sexually Transmitted ◽  
Nations Programme ◽  
Health Organization ◽  
A Prospective Study

The World Health Organization (WHO) and the Joint United Nations Programme on HIV and AIDS (UNAIDS) suggest that sexually transmitted infection (STI) surveillance should include other genital infections and not only human immunodeficiency virus (HIV). To monitor the concomitance of bacterial vaginosis (BV) and STIs in HIV-seropositive (HIV+) and HIV-seronegative (HIV–) patients, a prospective study was conducted in a cohort of 349 volunteers at a clinic specializing in treating STIs in Mexico City. Microbiological and molecular methods were used to detect STIs and dysbiosis in HIV+ and HIV– individuals. The prevalence of infection was higher in HIV+ (69.28%) than in HIV– (54.87%) individuals. BV was the most frequent infection in HIV+ individuals, and polymicrobial infections were 3 times more common in HIV+ individuals than in HIV– individuals (31.48 vs. 10.98%). Behaviors documented in a self-administered questionnaire included low condom use frequency in HIV+ individuals co-infected with BV or a STI. This finding highlights the importance of surveillance using routine microbiological evaluations for the correct management of genital infections in HIV+ patients because in the presence of HIV, the clinical presentations, courses, and therapeutic responses of some STIs can differ from those in patients without HIV infection.

scholarly journals Bacterial vaginosis

F1000Research ◽  
2017 ◽  
Vol 6 ◽  
pp. 1761 ◽  
Author(s):  
Phillip Hay
Keyword(s):  
Bacterial Vaginosis ◽  
Molecular Techniques ◽  
Women Of Childbearing Age ◽  
Childbearing Age ◽  
Dynamic Changes ◽  
Transmitted Infection ◽  
Sexually Transmitted ◽  
Health And Disease ◽  
Atopobium Vaginae

Bacterial vaginosis is the most prevalent cause of abnormal vaginal discharge in women of childbearing age. It can have a major impact on quality of life and psychological wellbeing if frequently recurrent and strongly symptomatic. The use of molecular techniques to study the vaginal microbiome is increasing our understanding of the dynamic changes in flora that occur in health and disease. It might soon be possible to separate Gardnerella into different pathogenic and non-pathogenic species. Many groups are studying compounds that can disrupt the biofilm which is dominated by Gardnerella and Atopobium vaginae. Several studies in the last decade support the concept of bacterial vaginosis as a sexually transmitted infection.

scholarly journals Bacterial vaginosis in pregnancy: distribution of bacterial species in different gram-stain categories of the vaginal flora

1996 ◽  
Vol 45 (2) ◽  
pp. 120-126 ◽  
Author(s):  
I. J. ROSENSTEIN ◽  
D. J. MORGAN ◽  
M. SHEEHAN ◽  
R. F. LAMONT ◽  
D. TAYLOR-ROBINSON
Keyword(s):  
Bacterial Vaginosis ◽  
Bacterial Species ◽  
Vaginal Flora ◽  
Gram Stain ◽  
In Pregnancy
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