scholarly journals Daytime of Sampling, Tooth-Brushing and Ascorbic Acid Influence Salivary Thiobarbituric Acid Reacting Substances – A Potential Clinical Marker of Gingival Status

2005 ◽  
Vol 21 (4) ◽  
pp. 203-207 ◽  
Author(s):  
Július Hodosy ◽  
Peter Celec
Keyword(s):  
Ascorbic Acid ◽  
Sialic Acid ◽  
Acid Content ◽  
Thiobarbituric Acid ◽  
Tooth Brushing ◽  
Gingival Tissue ◽  
Sialic Acid Content ◽  
Modified Method ◽  
Before And After ◽  
Pre Treatment

Background. Salivary thiobarbituric acid reacting substances (TBARS) have been previously shown to correlate with the impairment of gingival tissue. Although the details on the origin and the composition of this heterogeneous group of compounds in saliva are unknown, the potential clinical usefulness makes necessary the studies of factors influencing the salivary TBARS levels. Aim. To observe the effects of daily dynamics, tooth-brushing and ascorbic acid administration on salivary TBARS levels. Subjects and methods. Self-collected samples were obtained from 10 young healthy men collecting samples in the morning, in the afternoon and in the evening during 2 consecutive days. Ascorbic acid (250 mg) was administered orally after the last sampling on day 1 and before every sampling on day 2. Additional sampling was performed before and after tooth-brushing. TBARS levels in saliva specimens were detected spectrofluorometrically. Sialic acid content was measured using a modified method of Warren. Results. Salivary TBARS levels vary significantly during a day (p< 0.001) with highest concentrations in the morning. Both, tooth-brushing (p< 0.05) and short-term antioxidative treatment with ascorbic acid (p< 0.005) decrease salivary TBARS levels. Sialic acid content of saliva is not influenced significantly by any of the investigated factors. Conclusion. TBARS levels in saliva are affected by daytime of sampling, tooth-brushing and ascorbic acid pre-treatment. These results must be considered in clinical research using salivary TBARS levels. Sialic acid seems not to be a major component of TBARS in saliva. Further studies should clarify the molecular compounds of salivary TBARS and uncover the role of oral microbial factors.

Effect of desialylation of highly purified isoforms of human luteinizing hormone on their bioactivity in vitro, radioreceptor activity and immunoactivity

1997 ◽  
Vol 9 (5) ◽  
pp. 501 ◽  
Author(s):  
Patrick G. Burgon ◽  
Peter G. Stanton ◽  
Kim Pettersson ◽  
David M. Robertson
Keyword(s):  
Sialic Acid ◽  
Luteinizing Hormone ◽  
Acid Content ◽  
Specific Activity ◽  
Sialic Acid Residue ◽  
In Vitro Bioactivity ◽  
Human Pituitary ◽  
Sialic Acid Content ◽  
Before And After

To establish whether sialic acid content is responsible for an observed 7–8-fold variability in bioactivity in vitro of highly purified human pituitary luteinizing hormone (hLH) isoforms, the bioactivity in vitro, radioreceptor activity and immunoactivity of hLH isoforms were determined before and after enzymatic desialylation. Three immunofluorometric assays with different hLH specificities allowed characterization of 13–24 pituitary hLH isoform preparations of pI 7·03–8·98 in terms of sialic acid content (1–5 sialic acid residues per LH molecule), bioactivity in vitro (4030–30 000 I.U. mg-1), radioreceptor activity (6420–25 400 I.U. mg-1) and hLH immunoactivity (2900–4400 to 18 300–27 300 I.U. mg-1). Significant positive correlations between sialic acid content and either immunoactivity or in vitro bioactivity were observed, whereas radioreceptor activity showed a curvilinear response. Following more than 90% removal of sialic acid, both in vitro bioactivity and radioreceptor activity were increased, although specific activity still differed between isoforms; immunoactivities were unaffected. It is concluded that the presence of the sialic acid residue(s) on hLH isoforms does partially contribute to the in vitro bioactivity and radioreceptor activity of the isoforms, but that hLH immunoactivity is independent of sialic acid content.

SIALIC ACID CONTENT OF NORMAL RAT LIVER AND OF DAB-INDUCED HEPATOMATA

1964 ◽  
Vol 42 (1) ◽  
pp. 25-31 ◽  
Author(s):  
H. Kalant ◽  
Wilhelmina Mons ◽  
Marta Guttman
Keyword(s):  
Sialic Acid ◽  
Rat Liver ◽  
Acid Content ◽  
Thiobarbituric Acid ◽  
Normal Liver ◽  
Fresh Tissue ◽  
Sialic Acid Content ◽  
Tissue Samples ◽  
Total Sialic Acid ◽  
Normal Rat

Total sialic acid content of rat livers and tumors was determined by acid hydrolysis of tissue samples, isolation on Dowex-2 columns, and reaction with thiobarbituric acid. Normal rat liver was found to contain 465 μg of neuraminic acid per g of fresh tissue. In rats bearing hepatomata induced by the feeding of p-dimethylaminoazobenzene, the tumors contained an average of 1162 μg/g, while the surrounding liver contained 587 μg/g. Treatment of tissue sections with purified neuraminidase liberated only 27–28% of the sialic acid from liver tissue, but 49% of that in the hepatomata. Paper chromatographic analysis indicated a difference between normal liver and hepatomata with respect to the types of sialic acid present.

129: Sialic Acid Content of Urinary TAMM-Horsfall Protein is Reduced in Interstitial Cystitis Patients

2007 ◽  
Vol 177 (4S) ◽  
pp. 44-45
Author(s):  
C. Lowell Parsons ◽  
Mahadevan Rajasekaran ◽  
Marianne Chenoweth ◽  
Paul Stein
Keyword(s):  
Sialic Acid ◽  
Interstitial Cystitis ◽  
Acid Content ◽  
Sialic Acid Content

scholarly journals Differences in Sialic Acid Content of Human Interferons

1978 ◽  
Vol 41 (1) ◽  
pp. 175-178 ◽  
Author(s):  
J. Morser ◽  
J. P. Kabayo ◽  
D. W. Hutchinson
Keyword(s):  
Sialic Acid ◽  
Acid Content ◽  
Sialic Acid Content

scholarly journals Infection with Listeria monocytogenes impairs sialic acid addition to host cell glycoproteins.

1994 ◽  
Vol 180 (6) ◽  
pp. 2137-2145 ◽  
Author(s):  
M S Villanueva ◽  
C J Beckers ◽  
E G Pamer
Keyword(s):  
Listeria Monocytogenes ◽  
Sialic Acid ◽  
Host Cell ◽  
Mhc Class I ◽  
Acid Content ◽  
Class I ◽  
Sialic Acid Content ◽  
Infected Cells ◽  
The Impact ◽  
Glycosylation Defect

Listeria monocytogenes is a facultative intracellular bacterium that causes severe disease in neonates and immunocompromised adults. Although entry, multiplication, and locomotion of Listeria in the cytosol of infected cells are well described, the impact of such infection on the host cell is unknown. In this report, we investigate the effect of L. monocytogenes infection on MHC class I synthesis, processing, and intracellular trafficking. We show that L. monocytogenes infection interferes with normal processing of N-linked oligosaccharides on the major histocompatibility complex (MHC) class I heavy chain molecule, H-2Kd, resulting in a reduced sialic acid content. The glycosylation defect is more pronounced as the infection progresses and results from interference with the addition of sialic acid rather than its removal by a neuraminidase. The effect is found in two different cell lines and is not limited to MHC class I molecules since CD45, a surface glycoprotein, and LGP120, a lysosomal glycoprotein, are similarly affected by L. monocytogenes infection. The glycosylation defect is specific for infection by L. monocytogenes since neither Trypanosoma cruzi nor Yersinia enterocolitica, two other intracellular pathogens, reproduces the effect. The resultant hyposialylation of H-2Kd does not impair its surface expression in infected cells. Diminished sialic acid content of surface glycoproteins may enhance host-defense by increasing susceptibility to lysis and promoting clearance of Listeria-infected cells.

scholarly journals Erythrocyte Sialic Acid Content during Aging in Humans: Correlation with Markers of Oxidative Stress

2012 ◽  
Vol 32 (3) ◽  
pp. 179-186 ◽  
Author(s):  
Mohammad Murtaza Mehdi ◽  
Prabhakar Singh ◽  
Syed Ibrahim Rizvi
Keyword(s):  
Oxidative Stress ◽  
Sialic Acid ◽  
Erythrocyte Membrane ◽  
Acid Content ◽  
Lipid Hydroperoxide ◽  
Plasma Lipid ◽  
Sialic Acids ◽  
Sialic Acid Content ◽  
Total Antioxidant ◽  
The Relationship

Sialic acids are substituted neuraminic acid derivatives which are typically found at the outermost end of glycan chains on the membrane in all cell types. The role of erythrocyte membrane sialic acids during aging has been established however the relationship between sialic acid and oxidative stress is not fully understood. The present work was undertaken to analyze the relationship between erythrocyte membrane sialic acid with its plasma level, membrane and plasma lipid hydroperoxide levels and plasma total antioxidant capacity. Results show that sialic acid content decreases significantly (P< 0.001) in RBC membrane (r= −0.901) and increases in plasma (r= 0.860) as a function of age in humans. Lipid peroxidation measured in the form of hydroperoxides increases significantly (P< 0.001) in plasma (r= 0.830) and RBC membranes (r= 0.875) with age in humans. The Trolox Equivalent Total Antioxidant Capacity (TETAC) of plasma was found to be significantly decreased (P< 0.001,r= −0.844). We observe significant correlations between decrease of erythrocyte membrane sialic acid and plasma lipid hydroperoxide and TETAC. Based on the observed correlations, we hypothesize that increase in oxidative stress during aging may influence the sialic acid decomposition from membrane thereby altering the membrane configuration affecting many enzymatic and transporter activities. Considering the importance of plasma sialic acid as a diagnostic parameter, it is important to establish age-dependent reference.

Tamm-Horsfall glycoprotein: role in inhibition and promotion of renal calcium oxalate stone formation studied with Fourier-transform infrared spectroscopy

1994 ◽  
Vol 40 (9) ◽  
pp. 1739-1743 ◽  
Author(s):  
R Knörle ◽  
P Schnierle ◽  
A Koch ◽  
N P Buchholz ◽  
F Hering ◽  
...  
Keyword(s):  
Fourier Transform ◽  
Infrared Spectroscopy ◽  
Sialic Acid ◽  
Calcium Oxalate ◽  
Fourier Transform Infrared Spectroscopy ◽  
Acid Content ◽  
Stone Formation ◽  
Fourier Transform Infrared ◽  
Sialic Acid Content ◽  
Stone Formers

Abstract Tamm-Horsfall glycoprotein (THP) from healthy probands inhibits the precipitation of calcium oxalate, whereas THP from individuals who repeatedly develop calcium oxalate stones has no effect or even promotes precipitation. Using Fourier-transform infrared spectroscopy, we found a structural differentiation between these functionally different THPs: a decisive difference in sialic acid content. Quantitative analysis for sialic acid showed the same results. THP from healthy probands had a high sialic acid content (51 +/- 9 g/kg), whereas THP from recurrent stone formers had a decreased sialic acid content (21 +/- 4 g/kg). This explains the dual role of THP in the precipitation of calcium oxalate and the formation of renal stones and shows the importance of glycosylation in the function of this glycoprotein.

scholarly journals Role of Sialic Acid in the Dysfibrinogenemia Associated with Liver Disease

1977 ◽  
Author(s):  
J. Martinez ◽  
J. Palascak ◽  
D. Kwasniak ◽  
S.S. Shapiro
Keyword(s):  
Liver Disease ◽  
Sialic Acid ◽  
Acid Content ◽  
Polyacrylamide Gel Electrophoresis ◽  
Precipitation Method ◽  
Thrombin Time ◽  
Functional Abnormality ◽  
Sialic Acid Content ◽  
Fibrin Monomer

We have described an abnormal fibrinogen in 6 patients with liver disease who had prolonged plasma thrombin times due to impaired fibrin monomer aggregation. To investigate the role of sialic acid in this functional abnormality, fibrinogen was purified from normal and patient plasmas by the glycine precipitation method. Sialic acid content of the fibrinogens was measured by the thriobarbituric acid assay after acid hydrolysis. Normal fibrinogen had 6.1 ± 0.5 residues per molecule of fibrinogen, whereas patient fibrinogen sialic acid content ranged between 7.5 and 10 residues per molecule. The reduced fibrinogen demonstrated normal mobility of Aα, B3 and γ chains on SDS Polyacrylamide gel electrophoresis when stained for protein and, similar to normal fibrinogen, only the Bβ and γ chains stained with PAS. The degree of prolongation of the thrombin times of the purified patient fibrinogens appeared to correlate with the increase in the fibrinogen sialic acid. The effect on fibrin monomer aggregation of decreasing patient fibrinogen sialic acid content was studied. Partially desialated patient fibrinogen was prepared by treating the protein with Vibrio cholerae neuraminidase for varying periods of time. Partial removal of sialic acid from patient fibrinogen resulted in normalization of the thrombin time and improvement in fibrin monomer aggregation. Thrombin times ranged from 31.5 to 49.5 seconds prior to removal of excess sialic acid compared to 20.5 to 25.5 seconds post removal. These findings indicate that the dysfibrinogenemia associated with liver disease is biochemically characterized by increased sialic acid content and removal of this sialic acid results in a functional normalization of the protein.

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