scholarly journals DNA Ploidy and Liver Cell Dysplasia in Liver Biopsies from Patients with Liver Cirrhosis

2004 ◽  
Vol 18 (2) ◽  
pp. 87-91 ◽  
Author(s):  
Sayed S El-Sayed ◽  
Mohamed El-Sadany ◽  
Ashraf A Tabll ◽  
Ahmad Soltan ◽  
Ibrahim El-Dosoky ◽  
...  
Keyword(s):  
Liver Cirrhosis ◽  
Liver Cell ◽  
Clinical Laboratory ◽  
Dna Ploidy ◽  
Histopathological Examination ◽  
Dna Index ◽  
Dna Ploidy Pattern ◽  
Significant Difference ◽  
Liver Cell Dysplasia ◽  
Liver Biopsies

There is controversy among pathologists when assessing the presence or absence of liver cell dysplasia in liver biopsies taken from cirrhotic patients. The objective of the present study was to determine the DNA ploidy pattern of hepatocytes of patients with liver cirrhosis and its relationship to liver cell dysplasia. A total of 48 male patients diagnosed with liver cirrhosis based on clinical, laboratory and histopathological criteria were included in the study. A liver biopsy was taken from each patient; one part of the biopsy was subjected to histopathology, and the other to flow cytometry. The histopathological examination revealed liver cell dysplasia in 60% of patients with liver cirrhosis (62% of them had large cell dysplasia [LCD] and 38% had small cell dysplasia [SCD]). Abnormal DNA content (aneuploidy) was found in 81.5% of positive liver cell dysplasia specimens and found only in 11.1% of negative liver cell dysplasia specimens, with a statistically significant difference (P<0.001). Aneuploidy was found more commonly in LCD but without significant difference (P>0.05) in comparison with SCD. In conclusion, SCD (similar to LCD) is also associated with aneuploidy and elevated DNA index, and may carry the same risk for progression to hepatocellular carcinoma.

The Contribution of Sex Difference on Different Liver Histopathology Between Male and Female Mice After Oral Administration of Caffeine

2021 ◽  
Vol 2 (2) ◽  
pp. 37-41
Author(s):  
Muhammad Sholikhuddin Nafi’ ◽  
Tri Hartini Yuliawati ◽  
Prijati Sri Irawati ◽  
Nurina Hasanatuludhhiyah
Keyword(s):  
Oral Administration ◽  
Sex Difference ◽  
Liver Cell ◽  
Histopathological Examination ◽  
Treated Group ◽  
Male And Female ◽  
Female Mice ◽  
Liver Histopathology ◽  
End Of Treatment ◽  
Significant Difference

Background : There are several studies reporting the effect of caffeine on liver histopathology, but it remains controversy. The laboratory animal used in those studies were predominantly male, whereas there is contribution of sex difference on different liver reaction to xenobiotic between male and female subject. Objective : It is necessary to conduct a study to explore the differences between the liver histopathology of male and female mice after oral administration of caffeine. Methods : This study used 36 mice (Mus musculus) that were divided into 4 groups: male & female untreated groups and male & female treated groups which were orally administered with caffeine 0.4 mg / 20 gramBW daily for 30 days. At the end of treatment, mice were euthanized and dissected. Histopathological examination was done to determine the percentage of  liver cell death of each group. Results: The percentage of liver cell deathin female treated group was higher than male treated group (p = 0.0001). But there was no significant difference of liver cells death between male control and treated group and between female control and treated group. Conclusion : There was significant difference in liver histopathology between male and female mice after oral administration of caffeine.

AgNORs count and DNA ploidy in liver biopsies from patients with schistosomal liver cirrhosis and hepatocellular carcinoma

2009 ◽  
Vol 42 (16-17) ◽  
pp. 1616-1620 ◽  
Author(s):  
Abdelfattah M. Attallah ◽  
Ashraf A. Tabll ◽  
Eman El-Nashar ◽  
Kadry A. El-Bakry ◽  
Mohamed El-Sadany ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Liver Cirrhosis ◽  
Dna Ploidy ◽  
Liver Biopsies

DNA ploidy of liver biopsies from patients with liver cirrhosis and hepatocellular carcinoma: a flow cytometric analysis

1999 ◽  
Vol 142 (1) ◽  
pp. 65-69 ◽  
Author(s):  
Abdelfattah M Attallah ◽  
Ashraf A Tabll ◽  
Samia F Salem ◽  
Mohamed El-Sadany ◽  
Talaat A Ibrahim ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Liver Cirrhosis ◽  
Dna Ploidy ◽  
Flow Cytometric Analysis ◽  
Flow Cytometric ◽  
Liver Biopsies

scholarly journals Study of DNA Ploidy Pattern and DNA Index Using Flow Cytometry in Distant Metastasis of Gastric Cancer.

1993 ◽  
Vol 26 (10) ◽  
pp. 2395-2400
Author(s):  
Yukio Imanishi ◽  
Kazuyoshi Kurooka ◽  
Hiromi Yamada ◽  
Yoshinori Fujii ◽  
Suguru Sato ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Flow Cytometry ◽  
Distant Metastasis ◽  
Dna Ploidy ◽  
Dna Index ◽  
Dna Ploidy Pattern ◽  
Ploidy Pattern

scholarly journals Comparison of Histology and Clinical Variables to DNA Ploidy in Canine Mammary Tumors

1988 ◽  
Vol 25 (3) ◽  
pp. 219-226 ◽  
Author(s):  
E. Hellmén ◽  
A. Lindgren ◽  
F. Linell ◽  
P. Matsson ◽  
A. Nilsson
Keyword(s):  
Dna Analysis ◽  
Dna Ploidy ◽  
Mammary Tumors ◽  
Clinical Staging ◽  
Benign Tumors ◽  
Aneuploid Cell ◽  
Dna Index ◽  
Regional Lymph Nodes ◽  
Canine Mammary Tumors ◽  
Significant Difference

Flow cytometric DNA analysis was done on 132 canine mammary tumors from 99 dogs to evaluate the relation to histology and to clinical staging. Seventy-one tumors (54%) were histologically malignant; 38 (54%) of these were aneuploid and 33 (46%) were diploid. Fifty-two (39%) tumors were histologically benign, of which 45 (87%) were diploid and seven (13%) aneuploid. There were nine dysplastic mammae (7%); two were aneuploid and the rest diploid. DNA indices varied from 0.72 to 2.35. Of 58 mammary carcinomas, 25 (43%) were diploid and 33 (57%) were aneuploid (of the latter, 16 showed hypodiploidy and 17 hyperdiploidy with a predominance between DNA index 1.10 and 1.50). Three tumors (two carcinomas and one malignant mixed tumor) were multiploid with two aneuploid cell populations. The histological type varied within eight tumors, and in four of these the DNA index also varied. DNA indices varied within three tumors with uniform morphology. No correlation was found between DNA index and age of the dogs, nor between DNA index and tumor size. No significant differences were found between DNA index and histology, tumor growth pattern, or tumor location. Benign tumors were smaller than carcinomas, which were smaller than malignant mesenchymal tumors. Tumors growing adherent to the skin were larger than those not adherent to the skin. The regional lymph nodes were examined in 33 cases. No significant difference between the mean DNA index and presence of lymph node metastasis was found. These results show the possibility of using flow cytometry for DNA analysis in canine mammary tumors. Further investigations are needed to evaluate DNA ploidy as a diagnostic and prognostic tool in the investigation of canine mammary tumors.

AgNORs count and DNA ploidy in liver biopsies from patients with schistosomal liver cirrhosis and hepatocellular carcinoma [Clin. Biochem. 42 (2009) 1616–1620]

2016 ◽  
Vol 49 (12) ◽  
pp. 943
Author(s):  
Abdelfattah M. Attallah ◽  
Ashraf A. Tabll ◽  
Eman El-Nashar ◽  
Kadry A. El-Bakry ◽  
Mohamed El-Sadany ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Liver Cirrhosis ◽  
Dna Ploidy ◽  
Liver Biopsies

scholarly journals Levels and Significance of Serum Adiponectin in Patients with Chronic HBV Infection at Different Clinical Stages

2020 ◽  
Vol 185 ◽  
pp. 03008
Author(s):  
Bei Liu
Keyword(s):  
Liver Cirrhosis ◽  
Clinical Laboratory ◽  
High Serum ◽  
Hbv Infection ◽  
Control Group ◽  
Chronic Hbv Infection ◽  
Compensated Cirrhosis ◽  
Significant Difference ◽  
Clinical Stages ◽  
Chronic Hbv

To analyze the serum APN levels of patients with chronic HBV infection in different clinical stages and their correlation with clinical laboratory examination indicators. A total of 120 HBV-infected patients are included in this study, including chronic HBV carriers, chronic hepatitis B (CHB) and compensated cirrhosis patients, 40 cases in each group, and 40 medical examinees as healthy controls. Compared with the healthy control group, the APN level in the chronic HBV infection group is significantly increased (p<0.05), and the APN level in the cirrhosis group is significantly increased compared with the other two groups (p<0.05). There is no significant difference between the chronic HBV carrier group and the CHB group. The ratio of people with high APN in the high DNA viral load group is higher (p = 0.002, χ2 = 9.143); the APN level of the liver cirrhosis group is significantly different from the non-cirrhosis group (P = 0.004, χ2 = 8.123). There is no significant correlation between APN level and ALT, AST and other indicators (P>, 0.05). High serum APN may be used as a marker for the diagnosis of HBV-infected liver cirrhosis.

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