scholarly journals Single molecule Raman detection of enkephalin on silver colloidal particles

2004 ◽  
Vol 18 (3) ◽  
pp. 433-440 ◽  
Author(s):  
Katrin Kneipp ◽  
Harald Kneipp ◽  
Salim Abdali ◽  
Rolf W. Berg ◽  
Henrik Bohr
Keyword(s):  
Single Molecule ◽  
Building Block ◽  
Colloidal Particles ◽  
Raman Signal ◽  
Single Molecule Level ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Intrinsic Marker ◽  
Label Molecule ◽  
Specific Label

Enkephalin, an endogeneous substance in the human brain showing morphine‒like biological functions, has been detected at the single molecule level based on the surface‒enhanced Raman signal of the ring breathing mode of phenylalanine, which is one building block of the molecule. For enhancing the Raman signal the enkephalin molecules have been attached to silver colloidal cluster structures. The experiments demonstrate that the SERS signal of the strongly enhanced ring breathing vibration of phenylalanine at 1000 cm−1can be used as “intrinsic marker” for detecting a single enkephalin molecule without using a specific label molecule. The reported result suggests the use of the phenylalanine 1000 cm−1SERS line as spectroscopic signature for monitoring single proteins containing this amino acid as a building block.

scholarly journals Surface-enhanced Raman spectroscopy: benefits, trade-offs and future developments

2020 ◽  
Vol 11 (18) ◽  
pp. 4563-4577 ◽  
Author(s):  
Ana Isabel Pérez-Jiménez ◽  
Danya Lyu ◽  
Zhixuan Lu ◽  
Guokun Liu ◽  
Bin Ren
Keyword(s):  
Raman Spectroscopy ◽  
Single Molecule ◽  
Surface Enhanced Raman Spectroscopy ◽  
Direct Identification ◽  
Molecular Fingerprints ◽  
Single Molecule Level ◽  
Trade Offs ◽  
Target Analytes ◽  
Surface Enhanced ◽  
Surface Enhanced Raman

Surface-enhanced Raman spectroscopy (SERS) is a vibrational spectroscopy technique with sensitivity down to the single molecule level that provides fine molecular fingerprints, allowing for direct identification of target analytes.

Single-Molecule Level Rare Events Revealed by Dynamic Surface-Enhanced Raman Spectroscopy

2020 ◽  
Vol 92 (24) ◽  
pp. 15806-15810
Author(s):  
Cheng Zong ◽  
Chan-juan Chen ◽  
Xiang Wang ◽  
Pei Hu ◽  
Guo-kun Liu ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Single Molecule ◽  
Rare Events ◽  
Surface Enhanced Raman Spectroscopy ◽  
Dynamic Surface ◽  
Single Molecule Level ◽  
Surface Enhanced ◽  
Surface Enhanced Raman

scholarly journals Revealing unconventional host–guest complexation at nanostructured interface by surface-enhanced Raman spectroscopy

2021 ◽  
Vol 10 (1) ◽  
Author(s):  
Gan-Yu Chen ◽  
Yi-Bin Sun ◽  
Pei-Chen Shi ◽  
Tao Liu ◽  
Zhi-Hao Li ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Single Molecule ◽  
Inclusion Complexation ◽  
Surface Enhanced Raman Spectroscopy ◽  
Molecular Fingerprint ◽  
Au Nps ◽  
Single Molecule Level ◽  
Cooperative Adsorption ◽  
Surface Enhanced ◽  
Surface Enhanced Raman

AbstractInterfacial host–guest complexation offers a versatile way to functionalize nanomaterials. However, the complicated interfacial environment and trace amounts of components present at the interface make the study of interfacial complexation very difficult. Herein, taking the advantages of near-single-molecule level sensitivity and molecular fingerprint of surface-enhanced Raman spectroscopy (SERS), we reveal that a cooperative effect between cucurbit[7]uril (CB[7]) and methyl viologen (MV2+2I−) in aggregating Au NPs originates from the cooperative adsorption of halide counter anions I−, MV2+, and CB[7] on Au NPs surface. Moreover, similar SERS peak shifts in the control experiments using CB[n]s but with smaller cavity sizes suggested the occurrence of the same guest complexations among CB[5], CB[6], and CB[7] with MV2+. Hence, an unconventional exclusive complexation model is proposed between CB[7] and MV2+ on the surface of Au NPs, distinct from the well-known 1:1 inclusion complexation model in aqueous solutions. In summary, new insights into the fundamental understanding of host–guest interactions at nanostructured interfaces were obtained by SERS, which might be useful for applications related to host–guest chemistry in engineered nanomaterials.

Surface-Enhanced Raman Signal for Terbium Single-Molecule Magnets Grafted on Graphene

ACS Nano ◽  
2010 ◽  
Vol 4 (12) ◽  
pp. 7531-7537 ◽  
Author(s):  
Manuel Lopes ◽  
Andrea Candini ◽  
Matias Urdampilleta ◽  
Antoine Reserbat-Plantey ◽  
Valerio Bellini ◽  
...  
Keyword(s):  
Single Molecule ◽  
Raman Signal ◽  
Single Molecule Magnets ◽  
Surface Enhanced ◽  
Surface Enhanced Raman

scholarly journals Flexible SERS Sensors Based on Carbon Nanomaterials-Supported Au Nanostructures

2021 ◽  
Vol 6 (1) ◽  
pp. 7
Author(s):  
Rong Yang ◽  
Weichen Fang ◽  
Xiao Zuo ◽  
Igor M. De Rosa ◽  
Wenbo Xin
Keyword(s):  
Single Molecule ◽  
High Performance ◽  
Carbon Nanomaterials ◽  
Label Free ◽  
Single Molecule Level ◽  
Low Concentrations ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Enhanced Raman Scattering ◽  
Au Nanowires

Surface-enhanced Raman scattering (SERS) is a powerful technique to detect analytes in a label-free and non-destructive way at extremely low concentrations, even down to the single-molecule level. In the present study, a series of anisotropic Au nanostructures are integrated onto the platforms of carbon nanomaterials, mainly including carbon nanotubes (CNTs) and graphene, in order to fabricate high-performance flexible SERS sensors. Sizes, dimensions, and shapes of Au nanostructures can be well controlled through this strategy, based on which Au nanowires, nanoribbons, nanoplates, nanobelts, and nanoframes are successfully deposited onto CNT films and graphene templates, respectively. Significantly enhanced plasmonic activity originates from these Au nanocrystals, which provide increased SERS signals of the analytes by many orders of magnitude, while CNT films or graphene substrates offer superior flexibility and accessibility. For instance, A flexible SERS sensor made of graphene supported Au nanoframes can detect the analyte R6G at the concentration as low as 10−9 M. The mechanism for the sensitivity enhancement could be attributed to the homogenous distribution of Au nanoframes on the graphene support as well as the strong molecule adsorption to the graphene nanoporous network.

scholarly journals Rapid uropathogen identification using surface enhanced Raman spectroscopy active filters

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Simon D. Dryden ◽  
Salzitsa Anastasova ◽  
Giovanni Satta ◽  
Alex J. Thompson ◽  
Daniel R. Leff ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Bacterial Infections ◽  
Surface Enhanced Raman Spectroscopy ◽  
Active Filters ◽  
Raman Signal ◽  
Rapid Diagnostics ◽  
Bacterial Classification ◽  
Surface Enhanced ◽  
Surface Enhanced Raman

AbstractUrinary tract infection is one of the most common bacterial infections leading to increased morbidity, mortality and societal costs. Current diagnostics exacerbate this problem due to an inability to provide timely pathogen identification. Surface enhanced Raman spectroscopy (SERS) has the potential to overcome these issues by providing immediate bacterial classification. To date, achieving accurate classification has required technically complicated processes to capture pathogens, which has precluded the integration of SERS into rapid diagnostics. This work demonstrates that gold-coated membrane filters capture and aggregate bacteria, separating them from urine, while also providing Raman signal enhancement. An optimal gold coating thickness of 50 nm was demonstrated, and the diagnostic performance of the SERS-active filters was assessed using phantom urine infection samples at clinically relevant concentrations (105 CFU/ml). Infected and uninfected (control) samples were identified with an accuracy of 91.1%. Amongst infected samples only, classification of three bacteria (Escherichia coli, Enterococcus faecalis, Klebsiella pneumoniae) was achieved at a rate of 91.6%.

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