scholarly journals Cytologic and DNA-Cytometric Early Diagnosis of Oral Cancer

2001 ◽  
Vol 22 (4) ◽  
pp. 211-221 ◽  
Author(s):  
Torsten W. Remmerbach ◽  
Horst Weidenbach ◽  
Natalja Pomjanski ◽  
Kristiane Knops ◽  
Stefanie Mathes ◽  
...  
Keyword(s):  
Positive Predictive Value ◽  
Cancer Cells ◽  
Squamous Cell ◽  
Predictive Value ◽  
Early Identification ◽  
Image Cytometry ◽  
Oral Lesions ◽  
Dna Aneuploidy ◽  
Dna Image Cytometry

Objective. The aim of this prospective study was to report on the diagnostic accuracy of conventional oral exfoliative cytology taken from white‐spotted, ulcerated or other suspicious oral lesions in our clinic. In addition we checked DNA‐image cytometry as an adjuvant diagnostic tool. Our hypothesis is that DNA‐aneuploidy is a sensitive and specific marker for the early identification of tumor cells in oral brushings. Study design. 251 cytological diagnoses obtained from exfoliative smears of 181 patients from macroscopically suspicious lesions of the oral mucosa and from clinically seemingly benign oral lesions which were exisiced for establishing histological diagnoses were compared with histological and/or clinical follow‐ups of the respective patients. Additionally nuclear DNA‐contents were measured after Feulgen restaining using a TV image analysis system. Results. Sensitivity of our cytological diagnosis on oral smears for the detection of cancer cells was 94.6%, specificity 99.5%, positive predictive value 98.1% and negative predictive value 98.5%. DNA‐aneuploidy was assumed if abnormal DNA‐stemlines or cells with DNA‐content greater 9c were observed. On this basis the prevalence of DNA‐aneuploidy in smears of oral squamous cell carcinomasin situor invasive carcinomas was 96.4%. Sensitivity of DNA‐aneuploidy in oral smears for the detection of cancer cells was 96.4%, specificity 100%, positive predictive value 100% and negative 99.0%. The combination of both techniques increased the sensivity to 98.2%, specificity to 100%, positive predictive value to 100% and negative to 99.5%. Conclusions. Brush cytology of all visible oral lesions, if they are clinically considered as suspicious for cancer, are an easily practicable, cheap, non‐invasive, painless, safe and accurate screening method for detection of oral precancerous lesions, carcinomain situor invasive squamous cell carcinoma in all stages. We conclude that DNA‐image cytometry is a very sensitive, highly specific and objective adjuvant tool for the early identification of neoplastic epithelial cells in oral smears.

scholarly journals Immunocytochemistry and DNA-image Cytometry in Diagnostic Effusion Cytology. II. Diagnostic Accuracy in Equivocal Smears

1999 ◽  
Vol 19 (2) ◽  
pp. 59-66 ◽  
Author(s):  
Helma Motherby ◽  
Nicolaus Friedrichs ◽  
Mary Kube ◽  
Bahram Nadjari ◽  
Kristiane Knops ◽  
...  
Keyword(s):  
Positive Predictive Value ◽  
Diagnostic Accuracy ◽  
Predictive Value ◽  
Image Cytometry ◽  
Malignant Cells ◽  
Dna Cytometry ◽  
Dna Aneuploidy ◽  
Immunocytochemical Detection ◽  
Dna Image Cytometry

To determine sensitivity and specificity of different antibodies for the immunocytochemical detection of malignant cells in diagnostically equivocal effusions in comparison with those achieved by DNA‐image cytometry. 65 cytologically doubtful effusions of the serous cavities were stained with twelve antibodies. Furthermore, DNA‐image cytometry was performed. Data were correlated with patient follow‐up. Sensitivity of cellular staining of Ber‐EP4 for the identification of malignant cells was 77.8%, specificity of absent staining for benign cells was 100%. Positive predictive value for the identification of malignant cells was 100%, negative value 65.5%. Sensitivity of DNA‐aneuploidy for the identification of malignancy was 82.9%, specificity of DNA‐non‐aneuploidy for benignity 94.7%. The positive predictive value of DNA‐aneuploidy for the occurrence of malignant cells was 96.7%. Negative predictive value of DNA‐non‐aneuploidy was 72.0%. Combining immunocytochemistry applying Ber‐EP4 only and DNA‐cytometry in equivocal effusions resulted in a sensitivity of 88.9% for the identification of malignant cells associated with a 95.0% specificity. Positive predictive value was 97.7%, the negative one 79.2%.

scholarly journals Diagnostic DNA-Flow- vs. -Image-Cytometry in Effusion Cytology

2002 ◽  
Vol 24 (1) ◽  
pp. 5-15 ◽  
Author(s):  
Helma Motherby ◽  
Natalia Pomjanski ◽  
Mary Kube ◽  
Alexandra Boros ◽  
Thomas Heiden ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Positive Predictive Value ◽  
Tumor Cell ◽  
Negative Predictive Value ◽  
Sensitivity And Specificity ◽  
Predictive Value ◽  
Diagnostic Marker ◽  
Image Cytometry ◽  
Malignant Cells ◽  
Dna Aneuploidy

Aims: To determine the sensitivity and specificity of flow‐ and image‐cytometry for the detection of DNA‐aneuploidy as a marker for malignant cells in effusions.Methods: 200 effusions (80 tumor cell‐positive, 74 negative and 46 cytologically equivocal) were stained with DAPI‐SR for DNA‐flow‐ and with Feulgen‐Pararosaniline for ‐image‐cytometry. They were measured using a PAS‐flow‐cytometer and an AutoCyte‐QUIC‐DNA‐workstation according to the ESACP consensus reports for DNA‐flow‐ and ‐image‐cytometry, respectively [7,23,29,49].Results: Sensitivity of DNA‐aneuploidy for the identification of malignant cells was 32.1% for DNA‐flow‐ and 75.0% for ‐image‐cytometry, specificity of ‐euploidy in benign cells was 100.0% for both methods. Positive predictive value of DNA‐aneuploidy for the identification of malignant cells was 100.0% for both techniques, negative predictive value of DNA‐euploidy was 48.6% for DNA‐flow‐ and 72.0% for ‐image‐cytometry.Conclusions: Searching for DNA‐aneuploidy as a diagnostic marker for neoplastic cells in serous effusions image‐cytometry revealed superior sensitivity as compared with monoparametric flow cytometry.

scholarly journals Earliest Detection of Oral Cancer Using Non-Invasive Brush Biopsy Including DNA-Image-Cytometry: Report on Four Cases

2003 ◽  
Vol 25 (4) ◽  
pp. 159-166 ◽  
Author(s):  
Torsten W. Remmerbach ◽  
Horst Weidenbach ◽  
Alexander Hemprich ◽  
Alfred Böcking
Keyword(s):  
Oral Cancer ◽  
Case Reports ◽  
Image Cytometry ◽  
Severe Dysplasia ◽  
Brush Biopsy ◽  
Oral Cancers ◽  
Non Invasive ◽  
Dna Measurements ◽  
Dna Image Cytometry

Objective: We describe four patients presenting early oral cancers, detected cytologically on non‐invasive brush biopsies including DNA‐image cytometry as an adjunctive method before histology on scalpel biopsies confirmed the evidence of malignancy.Methods: Brush biopsies were performed and smears thereof investigated cytologically. After Feulgen restaining, DNA‐measurements were performed using a DNA‐Image‐Cytometer.Case reports: Oral squamous cell carcinomas were diagnosed cytologically in macroscopically suspicious lesions and malignancy confirmed by DNA‐cytometry. The initially performed scalpel biopsies did neither supply evidence of oral cancer nor of severe dysplasia. After at least one to 15 months the occurrence of cancer was finally proven histologically on a second scalpel biopsy each (three microinvasive and onein situcarcinoma).Conclusion: Non‐invasive brush biopsies are a suitable instrument for early cytologic detection of cancer of the mouth. DNA‐image‐cytometry, as an adjunctive method, can be used to confirm the cytologic diagnosis or suspicion of cancer in patients with doubtful lesions (dysplasias). DNA‐aneuploidy is a marker for (prospective) malignancy in smears of the oral cavity, which may detect malignancy months prior to histology. In future this method could be used as a mass screening tool in dentists practise. Colour figures can be viewed onhttp://www.esacp.org/acp/2003/25‐4/remmerbach.htm.

scholarly journals Automated Quantification of DNA Aneuploidy by Image Cytometry as an Adjunct for the Cytologic Diagnosis of Malignant Effusion

2013 ◽  
Vol 36 (3-4) ◽  
pp. 107-115 ◽  
Author(s):  
Zhilan Meng ◽  
Jie Shi ◽  
Chenyan Zhu ◽  
Jiangang Gu ◽  
Chen Zhou
Keyword(s):  
Image Cytometry ◽  
Cost Effective ◽  
Diagnostic Value ◽  
Youden Index ◽  
Malignant Effusion ◽  
Cytologic Diagnosis ◽  
Histologic Diagnosis ◽  
Dna Aneuploidy ◽  
Diploid Cells ◽  
Dna Image Cytometry

DNA aneuploidy is a cancer biomarker, which may have a potential diagnostic value in body effusion specimen. DNA aneuploidy is determined by measuring the DNA content of tested cells and comparing them with diploid cells (2c). In order to assess the value of automated DNA image cytometry (DNA-ICM) in the cytologic diagnosis of effusion, we measured DNA ploidy using an automated DNA-ICM analysis system in 126 consecutive effusion specimens and followed the cases for histologic diagnosis. Half of each effusion specimen was used to prepare cytologic smears for conventional cytologic diagnosis, while the other half was used to prepare a monolayer slide stained by Feulgen stain for automated ICM. By using Youden index, we found that 4 cells exceeding 2.5c is the optimal cut off value for aneuploidy, which has a sensitivity of 88.3% and specificity of 100% for diagnosis of malignant effusion. We also found that the DNA aneuploidy thresholds used for other types of cytologic specimens cannot be used in the diagnosis of effusion specimens. Our study demonstrated that automated DNA image cytometry is a simple, practical and cost-effective method for adjunct diagnosis of malignant effusion.

DNA image cytometry. A prognostic tool in squamous cell carcinoma of the esophagus?

Cancer ◽  
1991 ◽  
Vol 67 (9) ◽  
pp. 2290-2294 ◽  
Author(s):  
Thomas Böttger ◽  
Stefan Störkel ◽  
Michael Stöckle ◽  
Wolfgang Wahl ◽  
Michael Jugenheimer ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Image Cytometry ◽  
Carcinoma Of The Esophagus ◽  
Prognostic Tool ◽  
Dna Image Cytometry
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