scholarly journals New Algorithms Based on the Voronoi Diagram Applied in a Pilot Study on Normal Mucosa and Carcinomas

2000 ◽  
Vol 21 (2) ◽  
pp. 71-86 ◽  
Author(s):  
J. Sudbø ◽  
R. Marcelpoil ◽  
A. Reith
Keyword(s):  
Pilot Study ◽  
Oral Mucosa ◽  
Voronoi Diagram ◽  
Tongue Cancer ◽  
Ccd Camera ◽  
Structural Features ◽  
Grey Level ◽  
Tissue Architecture ◽  
Cell Nuclei ◽  
Cervical Carcinomas

An adequate reproducibility in the description of tissue architecture is still a challenge to diagnostic pathology, sometimes with unfortunate prognostic implications. To assess a possible diagnostic and prognostic value of quantitiative tissue architecture analysis, structural features based on the Voronoi Diagram (VD) and its subgraphs were developed and tested.A series of 27 structural features were developed and tested in a pilot study of 30 cases of prostate cancer, 10 cases of cervical carcinomas, 8 cases of tongue cancer and 8 cases of normal oral mucosa. Grey level images were acquired from hematoxyline‐eosine (HE) stained sections by a charge coupled device (CCD) camera mounted on a microscope connected to a personal computer (PC) with an image array processor. From the grey level images obtained, cell nuclei were automatically segmented and the geometrical centres of cell nuclei were computed. The resulting 2‐dimensional (2D) swarm of pointlike seeds distributed in a flat plane was the basis for construction of the VD and its subgraphs. From the polygons, triangulations and arborizations thus obtained, 27 structural features were computed as numerical values. Comparison of groups (normal vs. cancerous oral mucosa, cervical and prostate carcinomas with good and poor prognosis) with regard to distribution in the values of the structural features was performed with Student's t‐test.We demonstrate that some of the structural features developed are able to distinguish structurally between normal and cancerous oral mucosa (P=0.001), and between good and poor outcome groups in prostatic (P=0.001) and cervical carcinomas (P=0.001).We present results confirming previous findings that graph theory based algorithms are useful tools for describing tis‐ sue architecture (e.g., normal versus malignant). The present study also indicates that these methods have a potential for prognostication in malignant epithelial lesions.

scholarly journals Caveats: Numerical Requirements in Graph Theory Based Quantitation of Tissue Architecture

2000 ◽  
Vol 21 (2) ◽  
pp. 59-69 ◽  
Author(s):  
J. Sudbø ◽  
R. Marcelpoil ◽  
A. Reith
Keyword(s):  
Graph Theory ◽  
Structural Analysis ◽  
Oral Mucosa ◽  
Voronoi Diagram ◽  
Structural Features ◽  
Grey Level ◽  
Tissue Architecture ◽  
Cell Nuclei ◽  
Centre Of Gravity ◽  
Number Of Cells

Graph theory based methods represent one approach to an objective and reproducible structural analysis of tissue architecture. By these methods, neighborhood relations between a number of objects (e.g., cells) are explored and inherent to these methods are therefore certain requirements as to the number of objects to be included in the analysis. However, the question of how many objects are required to achieve reproducible values in repeated computations of proposed structural features, has previously not been adressed specifically.After digitising HE stained slides and storing them as grey level images, cell nuclei were segmented and their geometrical centre of gravity were computed, serving as the basis for construction of the Voronoi diagram (VD) and its subgraphs. Variations in repeated computations of structural features derived from these graphs were related to the number of cell nuclei included in the analysis.We demonstrate a large variation in the values of the structural features from one computation to another in one and the same section when only a limited number of cells (100–500) are included in the analysis. This variation decreased with increasing number of cells analyzed. The exact number of cells required to achieve reproducible values differ significantly between tissues, but not between separate cases of similar lesions. There are no significant differences between normal and malignantly changed tissues in oral mucosa with respect to how many cells must be included.For graph theory based analysis of tissue architecture, care must be taken to include an adequate number of objects; for some of the structural features we have tested, more than 3000 cells.

scholarly journals Contrast-enhanced ultrasound mapping of sentinel lymph nodes in oral tongue cancer—a pilot study

2017 ◽  
Vol 46 (3) ◽  
pp. 20160345 ◽  
Author(s):  
Shalva R Gvetadze ◽  
Ping Xiong ◽  
Mingming Lv ◽  
Jun Li ◽  
Jingzhou Hu ◽  
...  
Keyword(s):  
Pilot Study ◽  
Lymph Nodes ◽  
Tongue Cancer ◽  
Sentinel Lymph Nodes ◽  
Contrast Enhanced Ultrasound ◽  
Oral Tongue ◽  
Contrast Enhanced ◽  
Oral Tongue Cancer

Pilot study on laser propagation in maxillary and mandibular bone: Grey level image analysis for optical measurements

2017 ◽  
Vol 18 ◽  
pp. 226-231 ◽  
Author(s):  
Monalisa Jacob Guiselini ◽  
Alessandro Melo Deana ◽  
Daniela de Fátima Teixeira da Silva ◽  
Nelson Hideyoshi Koshoji ◽  
Raquel Agnelli Mesquita-Ferrari ◽  
...  
Keyword(s):  
Image Analysis ◽  
Pilot Study ◽  
Optical Measurements ◽  
Grey Level ◽  
Mandibular Bone ◽  
Laser Propagation ◽  
Grey Level Image

Deformation Characteristics of Wood Structures under Compression

2006 ◽  
Vol 505-507 ◽  
pp. 1051-1056 ◽  
Author(s):  
Keisuke Yamaguchi ◽  
M. Otsubo ◽  
Y. Motomura ◽  
Yasuo Marumo ◽  
Hiroyuki Shiozaki ◽  
...  
Keyword(s):  
Cell Structure ◽  
Ccd Camera ◽  
Structural Features ◽  
Deformation Characteristics ◽  
Compression Process ◽  
Long Distance ◽  
Wood Structures ◽  
Process Characteristics ◽  
Compression Direction ◽  
Grain Distribution

This study is aimed at investigating the deformation characteristics and mechanisms of wood and its deformation characteristics using long distance microscope and CCD-camera. The structures of actual wood can be treated as micro cell structure and layer one. Several deformation characteristics were obtained in compression process. Characteristics of the deformation were investigated experimentally. The following results were obtained for the deformation of wood. (1) The deformation modes of cell structures and the locations where deformation is generated were clarified. (2) The characteristics of the compression of layered structures were clarified. (3) The deformation of wood was largely affected by structural features such as the grain, distribution of tree rings, and compression direction. The results obtained are useful in carrying out effective compaction of wood.

scholarly journals Avaliação da biocompatibilidade do enxaguatório de Libidibia ferrea em mucosa bucal de ratos - estudo piloto / Evaluation of the biocompatibility of the Libidibia ferrea mouthwash in the oral mucosa of rats – a pilot study

2021 ◽  
Vol 7 (6) ◽  
pp. 60912-60927
Author(s):  
Raquel de Oliveira Marreiro ◽  
Wanderleia Monteiro de Souza ◽  
Tatiana Nayara Libório- Kimura ◽  
Tatiane Pereira de Souza ◽  
Maria Fulgência Costa Lima Bandeira ◽  
...  
Keyword(s):  
Pilot Study ◽  
Oral Mucosa

scholarly journals XANES studies of zirconia-ceria/Ni during partial/total methane oxidation

2014 ◽  
Vol 70 (a1) ◽  
pp. C130-C130
Author(s):  
Rebeca Bacani ◽  
Márcia Fantini ◽  
Tereza Martins ◽  
Susana Larrondo ◽  
Diego Lamas
Keyword(s):  
Methane Oxidation ◽  
Ccd Camera ◽  
Structural Features ◽  
Oxygen Storage ◽  
Total Oxidation ◽  
Oxidation Of Methane ◽  
Pluronic P123 ◽  
Release Capacity ◽  
Mesoporous Support ◽  
Temperature Programmed

Zirconia-ceria solid-solutions are extensively used as promoters for three-way catalysts, in addition, these materials can be used as anodes in solid oxide fuel cells (SOFCs) operated with hydrocarbons. The structural features of ZrO2-CeO2 materials in combination with oxygen storage/release capacity (OSC) are crucial for various catalytic reactions. The direct use of hydrocarbons as fuel for the SOFC (instead of pure H2), without the necessity of reforming and purification reactors can improve global efficiency of the system. The samples preparation method was developed using Zr and Ce chloride precursors, HCl aqueous solution, Pluronic P123, NH4OH and a Teflon autoclave. The samples were dried and calcined, until 540°C. The NiO impregnation was made with an ethanol dispersion of Ni(NO3)×6H2O, calcinated in air until 350°C for 2 hours. In-situ XANES experiments are capable to evaluate the reduction/oxidation potencial of Ni and Ce species in ZrO2-CeO2/Ni samples during partial/total methane oxidation and reduction reactions with H2. The experiments at the Ni K-edge/Ce L3-edge were collected at the LNLS D06A-DXAS beam line in transmission mode, using a Si(111) monochromator and a CCD camera as detector. The data were acquired during a series of temperature programmed reduction steps (TPR), under a 5% H2/He until 600°C, and mixtures of 20%CH4:5%O2/He with 2:1, 1:1 and 1:2 ratios. After each process with CH4 and O2, a TPR procedure was performed in order to evaluate the reduction capacity of the sample after reactions with CH4. The results demonstrated that NiO embedded in the porous ZrO2-CeO2 matrix, reduces at lower temperatures than standard NiO, measured in the same conditions, revealing that the mesoporous support improves the reduction of impregnated NiO. For both edges, there was formation of H2 during partial methane oxidation at 600°C. The total oxidation of methane was observed in lower temperatures (500°C). These results reveal that a high ceria content (90%) could be a great candidate for the SOFC anode.

Adjuvant antifungal therapy using tissue tolerable plasma on oral mucosa and removable dentures in oral candidiasis patients: a randomised double-blinded split-mouth pilot study

Mycoses ◽  
2016 ◽  
Vol 59 (7) ◽  
pp. 467-475 ◽  
Author(s):  
Saskia Preissner ◽  
Isabell Kastner ◽  
Eyke Schütte ◽  
Stefan Hartwig ◽  
Andrea Maria Schmidt-Westhausen ◽  
...  
Keyword(s):  
Pilot Study ◽  
Oral Mucosa ◽  
Antifungal Therapy ◽  
Oral Candidiasis ◽  
Double Blinded
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