scholarly journals Hydrolysis Profiles of Formalin Fixed Paraffin-Embedded Tumors Based on IOD (Integrated Optical Density) and Nuclear Texture Feature Measurements

1999 ◽  
Vol 19 (3-4) ◽  
pp. 111-118
Author(s):  
Margareta Fležar ◽  
Marija Us‐Krašovec ◽  
Mario žganec ◽  
Jaka Lavrenčak ◽  
Rastko Golouh
Keyword(s):  
Texture Feature ◽  
Texture Features ◽  
Serous Carcinoma ◽  
Hydrolysis Time ◽  
Formalin Fixed Paraffin ◽  
Integrated Optical ◽  
Formalin Fixed Paraffin Embedded ◽  
Serous Tumor ◽  
Borderline Malignancy ◽  
Nuclear Texture

The aim of the study was to determine optimal hydrolysis time for the Feulgen DNA staining of archival formalin fixed paraffin‐embedded surgical samples, prepared as single cell suspensions for image cytometric measurements. The nuclear texture features along with the IOD (integrated optical density) of the tumor nuclei were analysed by an automated high resolution image cytometer as a function of duration of hydrolysis treatment (in 5 N HCl at room temperature). Tissue blocks of breast carcinoma, ovarian serous carcinoma, ovarian serous tumor of borderline malignancy and leiomyosarcoma were included in the study. IOD hydrolysis profiles showed plateau between 30 and 60 min in the breast carcinoma and leiomyosarcoma, and between 40 and 60 min in the ovarian serous carcinoma and ovarian serous tumor of borderline malignancy. Most of the nuclear texture features remained stable after 20 min of hydrolysis treatment. Our results indicate that the optimal hydrolysis time for IOD and for nuclear texture feature measurements, was between 40 and 60 min in the cell preparations from tissue blocks of three epithelial and one soft tissue tumor.

scholarly journals Specific Changes of Chromatin Structure in Nuclei of Normal Epithelium Adjacent to Laryngeal Squamous Cell Carcinoma

2000 ◽  
Vol 20 (2-3) ◽  
pp. 141-150 ◽  
Author(s):  
Thomas Dreyer ◽  
Iris Knoblauch ◽  
David Garner ◽  
Alexei Doudkine ◽  
Calum MacAulay ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Laryngeal Squamous Cell Carcinoma ◽  
Texture Feature ◽  
Texture Features ◽  
Discriminant Functions ◽  
Paraffin Sections ◽  
Normal Epithelium ◽  
Integrated Optical ◽  
Nuclear Texture

The aim of this study was to confirm the existence of specific nuclear texture feature alterations of histologically normal epithelial borders nearby invasive laryngeal cancer (NC).Paraffin sections of NC and of chronic inflammations unrelated to cancer (CI) were analysed for nuclear texture and for integrated optical density (IOD‐index) and were compared to normal epithelium of patients without evidence of cancer (NE). Several discriminant functions based on nuclear texture features were trained to separate different subgroups.As the most important result, specific nuclear texture feature shifts were only found in NC with high‐density lymphocytic stroma infiltrate (NC+). Classification of nuclei of NE versus NC+ was correct in 70%. The same classifier was correct in only 58% when nuclei of NE were classified versus CI. We also found lower values of IOD‐Index within the NC+ group when compared to NE (p< 0:001).

scholarly journals Automatic Quantification of Immunohistochemically Stained Cell Nuclei Based on Standard Reference Cells

1998 ◽  
Vol 17 (2) ◽  
pp. 111-123 ◽  
Author(s):  
Petter Ranefall ◽  
Kenneth Wester ◽  
Ann-Catrin Andersson ◽  
Christer Busch ◽  
Ewert Bengtsson
Keyword(s):  
Cyclin A ◽  
Cell Nuclei ◽  
Cultured Fibroblasts ◽  
Automatic Quantification ◽  
Formalin Fixed Paraffin ◽  
Ki67 Antigen ◽  
Formalin Fixed Paraffin Embedded ◽  
Fully Automatic ◽  
Formalin Fixed ◽  
Nuclear Texture

A fully automatic method for quantification of images of immunohistochemically stained cell nuclei by computing area proportions, is presented. Agarose embedded cultured fibroblasts were fixed, paraffin embedded and sectioned at 4 µm. They were then stained together with 4 µm sections of the test specimen obtained from bladder cancer material.A colour based classifier is automatically computed from the control cells. The method was tested on formalin fixed paraffin embedded tissue section material, stained with monoclonal antibodies against the Ki67 antigen and cyclin A protein. Ki67 staining results in a detailed nuclear texture with pronounced nucleoli and cyclin A staining is obtained in a more homogeneously distributed pattern.However, different staining patterns did not seem to influence labelling index quantification, and the sensitivity to variations in light conditions and choice of areas within the control population was low. Thus, the technique represents a robust and reproducible quantification method.In tests measuring proportions of stained area an average standard deviation of about 1.5% for the same field was achieved when classified with classifiers created from different control samples.

A103 Immunostaining in the Diagnosis of Adrenal Cortical Tumors

2002 ◽  
Vol 126 (2) ◽  
pp. 170-172 ◽  
Author(s):  
Timothy S. Loy ◽  
Roy W. Phillips ◽  
Chadwick L. Linder
Keyword(s):  
Differential Diagnosis ◽  
Metastatic Carcinoma ◽  
Serous Carcinoma ◽  
Positive Staining ◽  
Adrenal Metastases ◽  
Cortical Cells ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Adrenal Cortical Cells ◽  
Formalin Fixed

Abstract Context.—The monoclonal antibody A103 recognizes an antigen on melanoma cells known as Melan-A or MART-1. Recent studies have shown that A103 also reacts with adrenal cortical cells and may be useful in the diagnosis of adrenal cortical tumors. However, only small numbers of some of the tumors in the differential diagnosis of adrenal cortical neoplasms have been studied. Objective.—To study the specificity of A103 immunohistochemistry in a large number of tumors in the differential diagnosis of adrenal cortical neoplasms. Design.—Formalin-fixed, paraffin-embedded tissue from 21 adrenal cortical tumors, 16 cases of metastatic carcinoma to the adrenal, 10 pheochromocytomas, and 269 extra-adrenal carcinomas was evaluated for A103 immunoreactivity using a commercially available antibody (Novocastra, Newcastle, UK). Results.—Positive staining was seen in all of the adrenal cortical tumors but in none of the adrenal metastases or pheochromocytomas. In the 269 extra-adrenal carcinomas, A103 immunoreactivity was limited to a single ovarian serous carcinoma. Conclusion.—A103 immunostaining is useful in distinguishing adrenal cortical neoplasms from other carcinomas and pheochromocytoma.

scholarly journals BRCA1 and MAD2 Are Coexpressed and Are Prognostic Indicators in Tubo-ovarian High-Grade Serous Carcinoma

2018 ◽  
Vol 28 (3) ◽  
pp. 472-478 ◽  
Author(s):  
Tara Byrne ◽  
Laura Nelson ◽  
James P. Beirne ◽  
Daniel Sharpe ◽  
Jennifer E. Quinn ◽  
...  
Keyword(s):  
Overall Survival ◽  
Staining Intensity ◽  
Prognostic Indicators ◽  
Serous Carcinoma ◽  
Nuclear Staining ◽  
High Grade ◽  
Strongly Correlated ◽  
Brca1 Expression ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded

ObjectivesThe aim of this study was to investigate the relationship between BRCA1 and mitotic arrest deficiency protein 2 (MAD2) protein expression, as determined by immunohistochemistry, and clinical outcomes in epithelial ovarian carcinoma (EOC).MethodsA tissue microarray consisting of 94 formalin-fixed paraffin-embedded EOC with fully matched clinicopathological data were immunohistochemically stained with anti-BRCA1 and anti-MAD2 antibodies. The cores were scored in a semiquantitative manner evaluating nuclear staining intensity and extent. Coexpression of BRCA1 and MAD2 was evaluated, and patient survival analyses were undertaken.ResultsCoexpression of BRCA1 and MAD2 was assessed in 94 EOC samples, and survival analysis was performed on 65 high-grade serous carcinomas (HGSCs). There was a significant positive correlation between BRCA1 and MAD2 expression in this patient cohort (P < 0.0001). Both low BRCA1 and low MAD2 are independently associated with overall survival because of HGSC. Low coexpression of BRCA1 and MAD2 was also significantly associated with overall survival and was driven by BRCA1 expression.ConclusionBRCA1 and MAD2 expressions are strongly correlated in EOC, but BRCA1 expression remains the stronger prognostic factor in HGSC.

Activation of EGFL7 within High Grade Ovarian Serous Carcinoma is Associated with Lower Tumor Infiltrating CD4+ and CD8+ Lymphocytes

2020 ◽  
Author(s):  
Jacek Jan Sznurkowski ◽  
Anton Żawrocki ◽  
Natalia Krawczyńska ◽  
Michał Bieńkowski ◽  
Bartosz Wasąg ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Immune Cell ◽  
Prognostic Significance ◽  
Intraepithelial Lymphocytes ◽  
Serous Carcinoma ◽  
High Grade ◽  
Ovarian Serous Carcinoma ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Epidermal Growth

Abstract It has been suggested that Epidermal Growth Factor like domain 7 (EFFL7) promotes tumor cell escape from immunity by downregulating the activation of tumor blood vessels. Aim: to analyze mRNA expression of EGFL7 within tumor microenvironment of high-grade ovarian serous carcinoma and its association with number of intraepithelial CD4+/CD8 + lymphocytes and expression of molecule involved in diapedesis ICAM-1. Methods: qPCR analysis of EGFL7 mRNA in cancer cells and adjacent stromal endothelium microdissected from formalin-fixed paraffin-embedded tumors of 59 high-grade ovarian serous carcinoma patients, was performed. Infiltration of intraepithelial lymphocytes (CD4+/CD8+) and expression of ICAM-1 were evaluated by immunohistochemistry and compared between tumors with different status of EGFL7 expression. Results: EGFL7 was expressed in cancer cells (9/59, 15.25%), endothelium (8/59, 13.56%), or both cancer cells and adjacent endothelium (4/59, 6.78%). ICAM-1 was expressed on cancer cells (47/59, 79.66%), stromal endothelium (46/59, 77.97%), or both epithelium and endothelium (40 of 59, 67.8%). EGFL7-positivity of cancer cells and endothelium was associated with lower intraepithelial inflow of CD4+ (p = 0.022 and p = 0.029, respectively) and CD8 + lymphocytes (p = 0.04 and p = 0.031, respectively) but impact neither epithelial nor endothelial ICAM-1 expression (p = 0.098 and p = 0.119, respectively). Patients’ median follow-up was 23.83 months (range 1.07–78.07). Lack of prognostic significance of EGFL7-status and ICAM-1 expression was notified. Conclusion: EGFL7 was independently activated in the cancer epithelium as frequently as in endothelium of human high-grade ovarian serous carcinoma. Activation of EGFL7 on cancer cells and/or endothelium impairs influx of immune cell effectors into cancer nest.

Sequencing Analysis and Phylogenetic Tree of HPV Isolated from Breast Cancer Patients at Thi-Qar Province/Iraq

2020 ◽  
pp. 37-40
Keyword(s):  
Nucleotide Sequence ◽  
Phylogenetic Tree ◽  
Evolutionary Relationship ◽  
Nucleotide Sequencing ◽  
Sequencing Analysis ◽  
Breast Cancer Patients ◽  
The North ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
History Of

Genetic variety examination has demonstrated fundamental to the understanding of the epidemiological and developmental history of Papillomavirus (HPV), for the development of accurate diagnostic tests and for efficient vaccine design. The HPV nucleotide diversity has been investigated widely among high-risk HPV types. To make the nucleotide sequence of HPV and do the virus database in Thi-Qar province, and compare sequences of our isolates with previously described isolates from around the world and then draw its phylogenetic tree, this study done. A total of 6 breast formalin-fixed paraffin-embedded (FFPE) of the female patients were included in the study, divided as 4 FFPE malignant tumor and 2 FFPE of benign tumor. The PCR technique was implemented to detect the presence of HPV in breast tissue, and the real-time PCR used to determinant HPV genotypes, then determined a complete nucleotide sequence of HPV of L1 capsid gene, and draw its phylogenetic tree. The nucleotide sequencing finding detects a number of substitution mutation (SNPs) in (L1) gene, which have not been designated before, were identified once in this study population, and revealed that the HPV16 strains have the evolutionary relationship with the South African race, while, the HPV33 and HPV6 showing the evolutionary association with the North American and East Asian race, respectively.

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