scholarly journals Detection of Human Papillomavirus Type 2 Related Sequence in Oral Papilloma

1998 ◽  
Vol 16 (3) ◽  
pp. 125-130 ◽  
Author(s):  
Taihei Yamaguchi ◽  
Masanobu Shindoh ◽  
Akira Amemiya ◽  
Nobuo Inoue ◽  
Masaaki Kawamura ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
Blot Hybridization ◽  
Southern Blot Hybridization ◽  
Hpv Infection ◽  
Related Sequence ◽  
Copy Numbers ◽  
Labeled Probe ◽  
Virus Capsid Antigen

Oral papilloma is a benign tumourous lesion. Part of this lesion is associated with human papillomavirus (HPV) infection. We analysed the genetical and histopathological evidence for HPV type 2 infection in three oral papillomas. Southern blot hybridization showed HPV 2a sequence in one lesion. Cells of the positive specimen appeared to contain high copy numbers of the viral DNA in an episomal state.In situstaining demonstrated virus capsid antigen in koilocytotic cells and surrounding cells in the hyperplastic epithelial layer. Two other specimens contained no HPV sequences by labeled probe of full length linear HPVs 2a, 6b, 11, 16, 18, 31 and 33 DNA under low stringency hybridization conditions. These results showed the possibility that HPV 2 plays a role in oral papilloma.

Human Papillomavirus (HPV) Cervical Lesions: Results from 300 Italian Women Studied with Dna Hybridization Techniques and Morphology

1988 ◽  
Vol 74 (6) ◽  
pp. 745-749 ◽  
Author(s):  
Fiorella Nuzzo ◽  
Vittorio Tison ◽  
Antonella Castagnoli ◽  
Maurizio Tiboni ◽  
Ethel-Michele De Villiers ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Human Papillomavirus ◽  
Southern Blot ◽  
Dna Hybridization ◽  
Blot Hybridization ◽  
Hpv Infection ◽  
Cervical Lesions ◽  
Hpv Dna ◽  
Cervical Infection

Human papillomavirus cervical infection was investigated in a series of 300 unselected women by comparing morphological diagnoses (cytology and histology) with results of DNA hybridization techniques (filter in situ hybridization of DNA from exfoliated cervical cells and Southern blot analysis of HPV-DNA in cervical biopsy specimens). The prevalence of HPV cervical infection diagnosed by PAP smears was 11.6 %. Despite disadvantages, filter in situ hybridization was confirmed to be particularly useful for screening purposes to detect HPV in cervical scrapings. In 3 cases it was the only applicable method for diagnosing « high-risk » HPV infection. Southern blot hybridization of tissue DNA with HPV 16-DNA revealed the presence of this virus in 8 cases, and HPV 31-DNA and HPV 42-DNA in 1 case each.

The Association of Human Papillomavirus Infection with Balanoposthitis: A Description of Five Cases with Proposals for Treatment

1994 ◽  
Vol 5 (2) ◽  
pp. 139-141 ◽  
Author(s):  
H D L Birley ◽  
G A Luzzi ◽  
M M Walker ◽  
B Ryait ◽  
D Taylor-Robinson ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
Blot Hybridization ◽  
Southern Blot Hybridization ◽  
Hpv Infection ◽  
Topical Steroids ◽  
Treatment Needs ◽  
Hpv Dna ◽  
Papillomavirus Infection ◽  
Oral Isotretinoin ◽  
Polymerase Chain

The features and clinical course of chronic balanitis in 5 patients are presented. In each case, histological examination of a cutaneous biopsy sample showed pronounced features of human papillomavirus (HPV) infection. In addition, HPV DNA was demonstrated in each biopsy specimen by a polymerase chain reaction and was found to be type 6 by Southern blot hybridization in 4 of the cases. Although the association of histological features of HPV infection with balanitis does not prove that HPV is causal, the failure to find other causes, the prolonged and distressing symptoms, and the ineffectiveness of topical steroids in improving symptoms, all suggest the importance of HPV infection. While effective treatment needs to be sought and developed, the response of one patient to oral isotretinoin suggests that this agent may be appropriate for a larger trial.

A combined ultrastructural and gene molecular research for the relationship between human uterine cervical carcinoma and human papillomavirus

1990 ◽  
Vol 48 (3) ◽  
pp. 204-205
Author(s):  
Kun Lee ◽  
Jingyi Si ◽  
Ricai Han ◽  
Wei Zhang ◽  
Bingbing Tan ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Human Papillomavirus ◽  
Intraepithelial Neoplasia ◽  
Hpv Infection ◽  
Dot Blot ◽  
Homologous Sequences ◽  
Normal Cervical Epithelium ◽  
The Relationship ◽  
Chronic Cervicitis

There are more supports for the view that human papillomavirus (HPV) infection might be an etiological factor in the development of cervical cancer when the association of persistent condylomata is considered. Biopsies from 318 cases with squamous cell carcinoma of uterine cervix, 48 with cervical and vulvar condylomata, 14 with cervical intraepithelial neoplasia (CIN), 34 with chronic cervicitis and 24 normal cervical epithelium were collected from 5 geographic regions of China with different cervical cancer mortalities. All specimens were prepared for Dot blot, Southern blot and in situ DNA-DNA hybridizations by using HPV-11, 16, 18 DNA labelled with 32P and 3H as probes to detect viral homologous sequences in samples. Among them, 32 cases with cervical cancer, 27 with condyloma and 10 normal cervical epitheliums were randomly chosen for comparative EM observation. The results showed that: 1), 192 out of 318 (60.4%) cases of cervical cancer were positive for HPV-16 DNA probe (Table I)

Human papillomavirus genotype as a major determinant of the course of cervical cancer.

1998 ◽  
Vol 16 (8) ◽  
pp. 2613-2619 ◽  
Author(s):  
I Lombard ◽  
A Vincent-Salomon ◽  
P Validire ◽  
B Zafrani ◽  
A de la Rochefordière ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Human Papillomavirus ◽  
Uterine Cervix ◽  
Blot Hybridization ◽  
Invasive Cervical Cancer ◽  
Disease Free Survival ◽  
Southern Blot Hybridization ◽  
Tumor Stage ◽  
Hpv Typing ◽  
Associated Tumors

PURPOSE To determine whether the prognosis of invasive cancers of the uterine cervix is related to the type of human papillomavirus (HPV) associated with the tumor. PATIENTS AND METHODS Two hundred ninety-seven patients with invasive cervical cancer were prospectively registered from 1986 to 1994. HPV typing was performed on DNA extracted from frozen tumor specimens by means of Southern blot hybridization (SBH) and polymerase chain reaction (PCR) techniques. The median follow-up was 38 months. RESULTS HPV sequences were detected in 246 patients (83%): 150 patients had HPV16, 31 patients had HPV18, and 14 patients had one of the intermediate-oncogenic-risk HPV types (HPV31, 33, 35, 52, 58). In 51 patients, HPV type remained undetermined, and in 51 patients, no viral sequences were found. No significant associations were observed between virologic data and tumor stage or node status. The 5-year disease-free survival (DFS) rate was 100% for patients with intermediate-risk HPV-associated tumors, 58% for patients with HPV16-positive tumors, and 38% for patients with HPV18-positive tumors (P = .02). In multivariate analysis, patients with HPV18-associated tumors had a relative risk (RR) of death 2.4 times greater (95% confidence interval [CI], 1.29-4.59) than that for patients with HPV16, and 4.4 times greater (95% CI, 3.48-5.32) than that for patients with a tumor associated with a viral type different from HPV16/18. CONCLUSION The prognosis for invasive cancers of the uterine cervix is dependent on the oncogenic potential of the associated HPV type. HPV typing may provide a prognostic indicator for individual patients and is of potential use in defining specific therapies against HPV-harboring tumor cells.

Human Papillomavirus DNA in Anal CarcinomasComparison of In Situ and Dot Blot Hybridization

1991 ◽  
Vol 96 (3) ◽  
pp. 318-325 ◽  
Author(s):  
MÁire A. Duggan ◽  
Valerie F. Boras ◽  
Masafumi Inoue ◽  
S. Elizabeth McGregor
Keyword(s):  
Human Papillomavirus ◽  
Blot Hybridization ◽  
Dot Blot ◽  
Dot Blot Hybridization ◽  
Papillomavirus Dna

scholarly journals Papillomas and Carcinomas Associated with a Papillomavirus in European Harvest Mice (Micromys minutus)

1988 ◽  
Vol 25 (5) ◽  
pp. 356-361 ◽  
Author(s):  
J. P. Sundberg ◽  
M. K. O'Banion ◽  
A. Shima ◽  
C. Knupp ◽  
M. E. Reichmann
Keyword(s):  
Blot Hybridization ◽  
Southern Blot Hybridization ◽  
Skin Lesions ◽  
Sebaceous Carcinoma ◽  
Cutaneous Lesions ◽  
Pulmonary Tumors ◽  
Papillomavirus Dna ◽  
Inverted Papillomas ◽  
Micromys Minutus

Papillomaviruses, group-specific papillomavirus antigens, or extrachromosomal papillomavirus DNA were detected in cutaneous, mucocutaneous, and pulmonary tumors affecting a colony of European harvest mice (Micromys minutus). Skin lesions were classified as acanthomatous hyperplasia, epidermal inclusion cysts. squamous papillomas, inverted papillomas, trichoepitheliomas, and sebaceous carcinomas. Cutaneous horns (hyperkeratotic papillomas) were on mucocutaneous junctions of one animal. One mouse, with a cutaneous sebaceous carcinoma, had multiple pulmonary keratinaceous cysts. Papillomavirus antigens, detected by the avidin-biotin technique, were in 20 of 31 lesions tested. In contrast, by Southern blot hybridization all 28 lesions tested contained papillomavirus DNA. Papillomavirus DNA was demonstrated in two often benign cutaneous lesions by in situ hybridization.

scholarly journals Fatal Epstein-Barr virus-associated hemophagocytic syndrome

Blood ◽  
1993 ◽  
Vol 82 (11) ◽  
pp. 3259-3264 ◽  
Author(s):  
H Kikuta ◽  
Y Sakiyama ◽  
S Matsumoto ◽  
T Oh-Ishi ◽  
T Nakano ◽  
...  
Keyword(s):  
Epstein Barr Virus ◽  
Hemophagocytic Syndrome ◽  
Blot Hybridization ◽  
Southern Blot Hybridization ◽  
Barr Virus ◽  
Clonality Analysis ◽  
Serologic Evidence ◽  
Infected Cells ◽  
Epstein Barr

A virus-associated hemophagocytic syndrome is characterized by high fever, liver dysfunction, coagulation abnormalities, pancytopenia, and a benign histiocytic proliferation with prominent hemophagocytosis in bone marrow, lymph node, spleen, and liver. We describe six Japanese children with fatal Epstein-Barr virus (EBV)-associated hemophagocytic syndrome. Five of the six patients had serologic evidence of primary EBV infection at the onset of their diseases. EBV genomes were detected in all the patients by Southern blot hybridization or the polymerase chain reaction. Furthermore, clonality analysis of the EBV genome showed that EBV-infected cells proliferated monoclonally or biclonally in three examined patients. In situ hybridization study using EBV- encoded RNA 1 (EBER1) showed that EBER1 was detected in one of two examined liver tissues, which localized in hepatocytes.

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