scholarly journals A Protein Binding Specifically to the IgG2b Switch Region

1997 ◽  
Vol 5 (2) ◽  
pp. 105-114 ◽  
Author(s):  
Heiner Völk ◽  
Matthias Wabl
Keyword(s):  
Affinity Chromatography ◽  
B Cell ◽  
Cell Line ◽  
Main Peak ◽  
Switch Region ◽  
Nuclear Extracts ◽  
Purification Scheme ◽  
Different Types ◽  
Abelson Virus ◽  
Dna Affinity Chromatography

The Abelson-virus-transformed mouse pre-B-cell line 18-81 switches almost exclusively from μ to γ2b. From nuclear extracts of this cell line, we have isolated a factor that specifically binds to Sγ2b. After an eight-step purification scheme, in which different types of DNA-affinity chromatography were used as key elements, we obtained a preparation with two narrowly spaced bands at approximately 69 kD on a silver-stained SDS gel. Binding specificity of main-peak fractions of affinity-purified proteins was analyzed by gel shift assays in which Sγ2b, but not Sμ, competes. The results are consistent with this factor being part of the switch recombinase.

scholarly journals Transformation-associated alterations in interactions between pre-B cells and fibronectin

Blood ◽  
1990 ◽  
Vol 76 (11) ◽  
pp. 2311-2320 ◽  
Author(s):  
FM Lemoine ◽  
S Dedhar ◽  
GM Lima ◽  
CJ Eaves
Keyword(s):  
B Cells ◽  
B Cell ◽  
Cell Line ◽  
Cell Lines ◽  
Stromal Cells ◽  
Stromal Cell ◽  
Cell Attachment ◽  
Attachment Site ◽  
Receptor Antibodies

Abstract Marrow stromal elements produce as yet uncharacterized soluble growth factors that can stimulate the proliferation of murine pre-B cells, although close contact between these two cell types appears to ensure a better pre-B cell response. We have now shown that freshly isolated normal pre-B cells (ie, the B220+, surface mu- fraction of adult mouse bone marrow) adhere to fibronectin (FN) via an RGD cell-attachment site, as shown in a serum-free adherence assay, and they lose this functional ability on differentiation in vivo into B cells (ie, the B220+, surface mu+ fraction). Similarly, cells from an immortalized but stromal cell-dependent and nontumorigenic murine pre-B cell line originally derived from a Whitlock-Witte culture were also found to adhere to fibronectin (FN) via an RGD cell-attachment site. Moreover, in the presence of anti-FN receptor antibodies, the ability of this immortalized pre-B cell line to proliferate when co-cultured with a supportive stromal cell line (M2–10B4 cells) was markedly reduced (down to 30% of control). This suggests that pre-B cell attachment to FN on stromal cells may be an important component of the mechanism by which stromal cells stimulate normal pre-B cell proliferation and one that is no longer operative to control their more differentiated progeny. Two differently transformed pre-B cell lines, both of which are autocrine, stromal-independent, tumorigenic in vivo, and partially or completely differentiation-arrested at a very early stage of pre-B cell development, did not bind to FN. In addition, anti-FN receptor antibodies were much less effective in diminishing the ability of these tumorigenic pre-B cells to respond to M2–10B4 cell stimulation, which could still be demonstrated when the tumorigenic pre-B cells were co- cultured with M2–10B4 cells at a sufficiently low cell density. Analysis of cell surface molecules immunoprecipitated from both the nontumorigenic and tumorigenic pre-B cell lines by an anti-FN receptor antibody showed an increase in very late antigen (VLA) alpha chain(s) in both tumorigenic pre-B cell lines and a decrease in the beta 1 chain in one. Interestingly, all of the pre-B cell lines expressed similar amounts of messenger RNA for the beta 1 chain of the FN receptor. These results suggest that alteration of FN receptor expression on pre-B cells may represent a mechanism contributing to the outgrowth of leukemic pre-B cells with an autocrine phenotype and capable of stromal cell-independent, autonomous growth.

scholarly journals Purification and characterization of class II histocompatibility antigens from a homozygous human B cell line.

1987 ◽  
Vol 262 (33) ◽  
pp. 16087-16094
Author(s):  
J C Gorga ◽  
V Horejsí ◽  
D R Johnson ◽  
R Raghupathy ◽  
J L Strominger
Keyword(s):  
B Cell ◽  
Cell Line ◽  
Class Ii ◽  
Purification And Characterization ◽  
Histocompatibility Antigens ◽  
Human B Cell

A human B cell line AF10 expressing hIL-17

IUBMB Life ◽  
1998 ◽  
Vol 45 (6) ◽  
pp. 1113-1119
Author(s):  
Limei Zhou ◽  
Shanyun Peng ◽  
Jubao Duan ◽  
Jing Zhou ◽  
Lihong Wang ◽  
...  
Keyword(s):  
B Cell ◽  
Cell Line ◽  
Human B Cell

scholarly journals Transcriptional activation of vesicular monoamine transporter 2 in the pre-B cell line Ea3.123

1999 ◽  
Vol 337 (2) ◽  
pp. 193 ◽  
Author(s):  
Fiona WATSON ◽  
Damian G. DEAVALL ◽  
Janet A. MACRO ◽  
Rachel KIERNAN ◽  
Rod DIMALINE
Keyword(s):  
B Cell ◽  
Cell Line ◽  
Transcriptional Activation ◽  
Vesicular Monoamine Transporter ◽  
Monoamine Transporter ◽  
Vesicular Monoamine Transporter 2

scholarly journals Inhibitor (IK) of IFN-γ induced HLA class II antigens expression also inhibits HLA class II constitutive expression in the human Raji B cell line

Oncogene ◽  
1997 ◽  
Vol 14 (12) ◽  
pp. 1453-1461 ◽  
Author(s):  
Jocelyn Vedrenne ◽  
Eric Assier ◽  
Raffaele Pereno ◽  
Haniaa Bouzinba-Segard ◽  
Bruno Azzarone ◽  
...  
Keyword(s):  
B Cell ◽  
Cell Line ◽  
Constitutive Expression ◽  
Class Ii ◽  
Hla Class Ii ◽  
Ifn Γ ◽  
Class Ii Antigens
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