scholarly journals Signal Amplification of FISH for Automated Detection Using Image Cytometry

1997 ◽  
Vol 13 (3) ◽  
pp. 137-146 ◽  
Author(s):  
Khuong Truong ◽  
Jack Boenders ◽  
Zofia Maciorowski ◽  
Philippe Vielh ◽  
Bernard Dutrillaux ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Human Lymphocytes ◽  
Ccd Camera ◽  
Image Cytometry ◽  
Automated Detection ◽  
Image Analyzer ◽  
Automated Scoring ◽  
Visual Scoring ◽  
Signal Characteristics ◽  
Amplification System

The purpose of this study was to improve the detection of FISH signals, in order that spot counting by a fully automated image cytometer be comparable to that obtained visually under the microscope. Two systems of spot scoring, visual and automated counting, were investigated in parallel on stimulated human lymphocytes with FISH using a biotinylated centromeric probe for chromosome 3. Signal characteristics were first analyzed on images recorded with a coupled charge device (CCD) camera. Number of spots per nucleus were scored visually on these recorded images versus automatically with a DISCOVERY image analyzer. Several fluochromes, amplification systems and pretreatments were tested. Our results for both visual and automated scoring show that the tyramide amplification system (TSA) gives the best amplification of signal if pepsin treatment is applied prior to FISH. Accuracy of the automated scoring, however, remained low (58% of nuclei containing two spots) compared to the visual scoring because of the high intranuclear variation between FISH spots.

Amplification systems of weak interaction biosensors: applications and prospects

Sensor Review ◽  
2015 ◽  
Vol 35 (1) ◽  
pp. 30-42 ◽  
Author(s):  
Xingya Wang ◽  
Guangchang Pang
Keyword(s):  
G Protein ◽  
Weak Interaction ◽  
Signal Amplification ◽  
Signaling Cascade ◽  
G Protein Signaling ◽  
Protein Signaling ◽  
Content Type ◽  
Amplification Method ◽  
Amplification System ◽  
Cascade Amplification

Purpose – This paper aims to provide a detailed review of weak interaction biosensors and several common biosensor methods for magnifying signals, as well as judiciously guide readers through selecting an appropriate detecting system and signal amplification method according to their research and application purpose. Design/methodology/approach – This paper classifies the weak interactions between biomolecules, summarizes the common signal amplification methods used in biosensor design and compares the performance of different kinds of biosensors. It highlights a potential electrochemical signal amplification method: the G protein signaling cascade amplification system. Findings – Developed biosensors which, based on various principles, have their own strengths and weaknesses have met the basic detection requirements for weak interaction between biomolecules: the selectivity, sensitivity and detection limit of biosensors have been consistently improving with the use of new signal amplification methods. However, most of the weak interaction biosensors stop at the research stage; there are only a minority realization of final commercial application. Originality/value – This paper evaluates the status of research and application of weak interaction biosensors systematically. The G protein signaling cascade amplification system proposal offers a new avenue for the research and development of electrochemical biosensors.

An enzymatic signal amplification system for calorimetric studies of cellobiohydrolases

2010 ◽  
Vol 404 (2) ◽  
pp. 140-148 ◽  
Author(s):  
Leigh Murphy ◽  
Martin J. Baumann ◽  
Kim Borch ◽  
Matt Sweeney ◽  
Peter Westh
Keyword(s):  
Signal Amplification ◽  
Amplification System ◽  
Calorimetric Studies ◽  
Enzymatic Signal Amplification ◽  
Signal Amplification System
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