scholarly journals The Ontogeny of Chicken Bursal Stromal Cells Defined by Monoclonal Antibodies

1990 ◽  
Vol 1 (1) ◽  
pp. 31-39 ◽  
Author(s):  
Trevor J. Wilson ◽  
Richard L. Boyd
Keyword(s):  
Monoclonal Antibodies ◽  
B Cell ◽  
Stromal Cells ◽  
Preliminary Evidence ◽  
Bursa Of Fabricius ◽  
Surface Epithelium ◽  
Secretory Cells ◽  
B Cell Differentiation ◽  
Ontogenic Development ◽  
Tunica Propria

Molecules expressed on lymphoid stromal cells influence the differentiation of lymphocytes. We have examined the expression of stromal markers, identified by monoclonal antibodies, in the chicken bursa of Fabricius during ontogenic development. These results are also consistent with the hypothesis that medullary secretory cells are of mesenchymal origin, whereas the basement membrane-associated and some medullary epithelium are derived from the endoderm. Our results have demonstrated the complexity of bursal stromal development with determinants expressed on the adult medullary stellate cells (e.g., MUI-57 and 62) and cortical macrophages (e.g., MUI-66 and 72) detected on the early em.bryonic tunica propria (e.g., MUI-57, 66 and 72) or surface epithelium (e.g., MUI-62 and 66). In addition, we provide preliminary evidence regarding potential functions of these molecules in stem cell colonization (MUI-52), early B-cell differentiation (e.g., MUI-72), late bursal B-cell development (MUI-69 and 71) and the follicle-associated epithelium transport mechanism (MUI-61 and 73).

scholarly journals The immunomodulatory functions and molecular mechanism of a new bursal heptapeptide (BP7) in immune responses and immature B cells

2019 ◽  
Vol 50 (1) ◽  
Author(s):  
Xiu Li Feng ◽  
Yang Zheng ◽  
Man Man Zong ◽  
Shan Shan Hao ◽  
Guang Fang Zhou ◽  
...  
Keyword(s):  
B Cells ◽  
Immune Responses ◽  
B Cell ◽  
Molecular Mechanisms ◽  
Bursa Of Fabricius ◽  
Biological Factor ◽  
Mrna Levels ◽  
B Cell Differentiation ◽  
Immune Functions ◽  
Wehi 231

Abstract The bursa of Fabricius (BF) is the acknowledged central humoural immune organ unique to birds and plays a vital role in B lymphocyte development. In addition, the unique molecular immune features of bursal-derived biological peptides involved in B cell development are rarely reported. In this paper, a novel bursal heptapeptide (BP7) with the sequence GGCDGAA was isolated from the BF and was shown to enhance the monoclonal antibody production of a hybridoma. A mouse immunization experiment showed that mice immunized with an AIV antigen and BP7 produced strong antibody responses and cell-mediated immune responses. Additionally, BP7 stimulated increased mRNA levels of sIgM in immature mouse WEHI-231 B cells. Gene microarray results confirmed that BP7 regulated 2465 differentially expressed genes in BP7-treated WEHI-231 cells and induced 13 signalling pathways and various immune-related functional processes. Furthermore, we found that BP7 stimulated WEHI-231 cell autophagy and AMPK-ULK1 phosphorylation and regulated Bcl-2 protein expression. Finally, chicken immunization showed that BP7 enhanced the potential antibody and cytokine responses to the AIV antigen. These results suggested that BP7 might be an active biological factor that functions as a potential immunopotentiator, which provided some novel insights into the molecular mechanisms of the effects of bursal peptides on immune functions and B cell differentiation.

scholarly journals Support of early B-cell differentiation in mouse fetal liver by stromal cells and interleukin-7

Blood ◽  
1991 ◽  
Vol 77 (12) ◽  
pp. 2612-2617 ◽  
Author(s):  
Y Gunji ◽  
T Sudo ◽  
J Suda ◽  
Y Yamaguchi ◽  
H Nakauchi ◽  
...  
Keyword(s):  
B Cell ◽  
Cell Line ◽  
Stromal Cells ◽  
Stromal Cell ◽  
Fetal Liver ◽  
Liver Cells ◽  
B Cell Differentiation ◽  
Stromal Cell Line ◽  
Fetal Liver Cells

We compared the development of B-cell progenitors with that of myeloid progenitors in fetal liver cells at various gestational ages. Day 12 to 14 fetal liver cells did not form pre-B-cell colonies. Pre-B-cell colonies were developed from day 15 fetal liver cells. The incidence of colonies increased with increases in gestational age and reached a maximum on days 18 to 19. In contrast, the incidence of myeloid colonies formed in the presence of interleukin-3 (IL-3) and erythropoietin did not change significantly during days 13 to 21 of gestation. After coculturing day 13 fetal liver cells with IL-7- producing stromal cell line ST-2, they could respond to IL-7 and proliferate. Analysis of the phenotypes showed that day 13 fetal liver cells were B220-, IgM-, while culturing day 13 fetal liver cells with ST-2 and untreated day 18 fetal liver cells contained the population of B220+ cells. Even in the presence of IL-7-defective stromal cell line FLS-3, IL-7-responsive cells could be induced from day 13 fetal liver cells. IL-7 acted on B220+ cells and induced pre-B-cell colonies that contained IgM+ cells in the methylcellulose culture. IL-7 mRNA was expressed in days 13 and 18 fetal liver cells but not in pre-B cells or adult liver cells. From these findings, it is suggested that stromal cells or stromal-derived factors but not IL-7 were required for the differentiation from B220- cells to B220+ cells. In the second stage, B220+, IgM- cells proliferated and some of them differentiated to IgM+ cells in the presence of IL-7 alone. The two-step model can apply to in vivo early B lymphopoiesis.

scholarly journals Support of early B-cell differentiation in mouse fetal liver by stromal cells and interleukin-7

Blood ◽  
1991 ◽  
Vol 77 (12) ◽  
pp. 2612-2617 ◽  
Author(s):  
Y Gunji ◽  
T Sudo ◽  
J Suda ◽  
Y Yamaguchi ◽  
H Nakauchi ◽  
...  
Keyword(s):  
B Cell ◽  
Cell Line ◽  
Stromal Cells ◽  
Stromal Cell ◽  
Fetal Liver ◽  
Liver Cells ◽  
B Cell Differentiation ◽  
Stromal Cell Line ◽  
Fetal Liver Cells

Abstract We compared the development of B-cell progenitors with that of myeloid progenitors in fetal liver cells at various gestational ages. Day 12 to 14 fetal liver cells did not form pre-B-cell colonies. Pre-B-cell colonies were developed from day 15 fetal liver cells. The incidence of colonies increased with increases in gestational age and reached a maximum on days 18 to 19. In contrast, the incidence of myeloid colonies formed in the presence of interleukin-3 (IL-3) and erythropoietin did not change significantly during days 13 to 21 of gestation. After coculturing day 13 fetal liver cells with IL-7- producing stromal cell line ST-2, they could respond to IL-7 and proliferate. Analysis of the phenotypes showed that day 13 fetal liver cells were B220-, IgM-, while culturing day 13 fetal liver cells with ST-2 and untreated day 18 fetal liver cells contained the population of B220+ cells. Even in the presence of IL-7-defective stromal cell line FLS-3, IL-7-responsive cells could be induced from day 13 fetal liver cells. IL-7 acted on B220+ cells and induced pre-B-cell colonies that contained IgM+ cells in the methylcellulose culture. IL-7 mRNA was expressed in days 13 and 18 fetal liver cells but not in pre-B cells or adult liver cells. From these findings, it is suggested that stromal cells or stromal-derived factors but not IL-7 were required for the differentiation from B220- cells to B220+ cells. In the second stage, B220+, IgM- cells proliferated and some of them differentiated to IgM+ cells in the presence of IL-7 alone. The two-step model can apply to in vivo early B lymphopoiesis.

Regulation of B-cell differentiation by stromal cells

1995 ◽  
pp. 103-128 ◽  
Author(s):  
Elizabeth A. Faust ◽  
Douglas C. Saffran ◽  
Owen N. Witte
Keyword(s):  
Cell Differentiation ◽  
B Cell ◽  
Stromal Cells ◽  
B Cell Differentiation

B-C1, B-C2, B-C3: Monoclonal Antibodies against B Cell Differentiation Antigens

1986 ◽  
pp. 121-139 ◽  
Author(s):  
Ignacio Anegón ◽  
Ramón Vilella ◽  
Teresa Gallart ◽  
Cristina Cuturi ◽  
Luis Borche ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Cell Differentiation ◽  
B Cell ◽  
B Cell Differentiation ◽  
Differentiation Antigens

TPA-Induced Modulation of B Cell Differentiation Antigens Defined by Monoclonal Antibodies (HD6, HD28, HD37, HD39)

1986 ◽  
pp. 527-540 ◽  
Author(s):  
Reinhard Schwartz ◽  
Gerhard Moldenhauer ◽  
Bernd Dörken ◽  
Antonio Pezzutto ◽  
Frank Momburg ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Cell Differentiation ◽  
B Cell ◽  
B Cell Differentiation ◽  
Differentiation Antigens
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