scholarly journals Epithelial Sodium Transport and Its Control by Aldosterone: The Story of Our Internal Environment Revisited

2015 ◽  
Vol 95 (1) ◽  
pp. 297-340 ◽  
Author(s):  
Bernard C. Rossier ◽  
Michael E. Baker ◽  
Romain A. Studer
Keyword(s):  
Sodium Transport ◽  
Water Conservation ◽  
Ionic Composition ◽  
Basolateral Membrane ◽  
Extracellular Fluid ◽  
High Concentration ◽  
Regulatory Pathways ◽  
Extracellular Milieu ◽  
Epithelial Barriers ◽  
Set Up

Transcription and translation require a high concentration of potassium across the entire tree of life. The conservation of a high intracellular potassium was an absolute requirement for the evolution of life on Earth. This was achieved by the interplay of P- and V-ATPases that can set up electrochemical gradients across the cell membrane, an energetically costly process requiring the synthesis of ATP by F-ATPases. In animals, the control of an extracellular compartment was achieved by the emergence of multicellular organisms able to produce tight epithelial barriers creating a stable extracellular milieu. Finally, the adaptation to a terrestrian environment was achieved by the evolution of distinct regulatory pathways allowing salt and water conservation. In this review we emphasize the critical and dual role of Na+-K+-ATPase in the control of the ionic composition of the extracellular fluid and the renin-angiotensin-aldosterone system (RAAS) in salt and water conservation in vertebrates. The action of aldosterone on transepithelial sodium transport by activation of the epithelial sodium channel (ENaC) at the apical membrane and that of Na+-K+-ATPase at the basolateral membrane may have evolved in lungfish before the emergence of tetrapods. Finally, we discuss the implication of RAAS in the origin of the present pandemia of hypertension and its associated cardiovascular diseases.

Axonal Function and Ionic Regulation in the Central Nervous System of a Phytophagous Insect (Carausius Morosus)

1967 ◽  
Vol 47 (2) ◽  
pp. 235-247
Author(s):  
J. E. TREHERNE ◽  
S. H. P. MADDRELL
Keyword(s):  
Ionic Composition ◽  
Extracellular Fluid ◽  
Extracellular Recording ◽  
Inorganic Ions ◽  
Sodium Ions ◽  
Phytophagous Insect ◽  
High Concentration ◽  
Action Current ◽  
Local Regulation ◽  
The Central Nervous System

1. Experiments vising intracellular and extracellular recording techniques indicate that, despite the specialized ionic composition of the haemolymph, the axons in the nerve cord of Carausius are conventional in that the action current is largely carried by sodium ions. 2. This effect is achieved by an appreciable regulation of the concentrations of inorganic ions in the extracellular fluid bathing the axon surfaces. 3. The extra-axonal regulation does not appear to result from any significant restriction in the accessibility of cations to the general extracellular system, but from a local regulation which appears to maintain a relatively high concentration of sodium ions at the axon surfaces. 4. It is suggested that such a regulation may be achieved by an extrusion of sodium ions from the glial cells into the restricted extra-axonal spaces demonstrated in the electron micrographs of this preparation.

PBA increases CFTR expression but at high doses inhibits Cl−secretion in Calu-3 airway epithelial cells

1999 ◽  
Vol 277 (4) ◽  
pp. L700-L708 ◽  
Author(s):  
Johannes Loffing ◽  
Bryan D. Moyer ◽  
Donna Reynolds ◽  
Bruce A. Stanton
Keyword(s):  
Protein Expression ◽  
Apical Membrane ◽  
Basolateral Membrane ◽  
Airway Epithelial Cells ◽  
Cell Phenotype ◽  
Apical Plasma Membrane ◽  
Airway Epithelial ◽  
High Concentration ◽  
Cycle Enzyme ◽  
Cf Transmembrane Conductance Regulator

Sodium 4-phenylbutyrate (PBA), a short-chain fatty acid, has been approved to treat patients with urea cycle enzyme deficiencies and is being evaluated in the management of sickle cell disease, thalassemia, cancer, and cystic fibrosis (CF). Because relatively little is known about the effects of PBA on the expression and function of the wild-type CF transmembrane conductance regulator (wt CFTR), the goal of this study was to examine the effects of PBA and related compounds on wt CFTR-mediated Cl−secretion. To this end, we studied Calu-3 cells, a human airway cell line that expresses endogenous wt CFTR and has a serous cell phenotype. We report that chronic treatment of Calu-3 cells with a high concentration (5 mM) of PBA, sodium butyrate, or sodium valproate but not of sodium acetate reduced basal and 8-(4-chlorophenylthio)-cAMP-stimulated Cl−secretion. Paradoxically, PBA enhanced CFTR protein expression 6- to 10-fold and increased the intensity of CFTR staining in the apical plasma membrane. PBA also increased protein expression of Na+-K+-ATPase. PBA reduced CFTR Cl−currents across the apical membrane but had no effect on Na+-K+-ATPase activity in the basolateral membrane. Thus a high concentration of PBA (5 mM) reduces Cl−secretion by inhibiting CFTR Cl−currents across the apical membrane. In contrast, lower therapeutic concentrations of PBA (0.05–2 mM) had no effect on cAMP-stimulated Cl−secretion across Calu-3 cells. We conclude that PBA concentrations in the therapeutic range are unlikely to have a negative effect on Cl−secretion. However, concentrations >5 mM might reduce transepithelial Cl−secretion by serous cells in submucosal glands in individuals expressing wt CFTR.

scholarly journals Volatility of secondary organic aerosol during OH radical induced ageing

2011 ◽  
Vol 11 (21) ◽  
pp. 11055-11067 ◽  
Author(s):  
K. Salo ◽  
M. Hallquist ◽  
Å. M. Jonsson ◽  
H. Saathoff ◽  
K.-H. Naumann ◽  
...  
Keyword(s):  
Gas Phase ◽  
Organic Aerosol ◽  
Oh Radical ◽  
High Concentration ◽  
Volatile Material ◽  
Phase Oxidation ◽  
Gas Phase Oxidation ◽  
Institute Of Technology ◽  
Set Up ◽  
Pinic Acid

Abstract. The aim of this study was to investigate oxidation of SOA formed from ozonolysis of α-pinene and limonene by hydroxyl radicals. This paper focuses on changes of particle volatility, using a Volatility Tandem DMA (VTDMA) set-up, in order to explain and elucidate the mechanism behind atmospheric ageing of the organic aerosol. The experiments were conducted at the AIDA chamber facility of Karlsruhe Institute of Technology (KIT) in Karlsruhe and at the SAPHIR chamber of Forchungzentrum Jülich (FZJ) in Jülich. A fresh SOA was produced from ozonolysis of α-pinene or limonene and then aged by enhanced OH exposure. As an OH radical source in the AIDA-chamber the ozonolysis of tetramethylethylene (TME) was used while in the SAPHIR-chamber the OH was produced by natural light photochemistry. A general feature is that SOA produced from ozonolysis of α-pinene and limonene initially was rather volatile and becomes less volatile with time in the ozonolysis part of the experiment. Inducing OH chemistry or adding a new portion of precursors made the SOA more volatile due to addition of new semi-volatile material to the aged aerosol. The effect of OH chemistry was less pronounced in high concentration and low temperature experiments when lower relative amounts of semi-volatile material were available in the gas phase. Conclusions drawn from the changes in volatility were confirmed by comparison with the measured and modelled chemical composition of the aerosol phase. Three quantified products from the α-pinene oxidation; pinonic acid, pinic acid and methylbutanetricarboxylic acid (MBTCA) were used to probe the processes influencing aerosol volatility. A major conclusion from the work is that the OH induced ageing can be attributed to gas phase oxidation of products produced in the primary SOA formation process and that there was no indication on significant bulk or surface reactions. The presented results, thus, strongly emphasise the importance of gas phase oxidation of semi- or intermediate-volatile organic compounds (SVOC and IVOC) for atmospheric aerosol ageing.

Adaptation of distal tubule and collecting duct to increased Na delivery. II. Na+ and K+ transport

1988 ◽  
Vol 255 (6) ◽  
pp. F1269-F1275 ◽  
Author(s):  
B. A. Stanton ◽  
B. Kaissling
Keyword(s):  
Tap Water ◽  
Collecting Duct ◽  
Basolateral Membrane ◽  
Extracellular Fluid ◽  
Distal Tubule ◽  
Sodium Absorption ◽  
Transport Capacity ◽  
Volume Depletion ◽  
Osmotic Minipump ◽  
Sodium Uptake

This study was conducted to determine whether a chronic increase in sodium delivery to, and sodium uptake by, the distal tubule stimulates the transport capacity of this tubular segment. To increase the rate of sodium delivery to the distal tubule, furosemide (12 mg/day) was administered continuously to rats by osmotic minipump for 6 days. Volume depletion was prevented by giving the animals a drinking solution containing 0.8% NaCl and 0.1% KCl. Control animals were given vehicle (0.9% NaCl) by osmotic minipump and tap water to drink. All animals were adrenalectomized and given replacement doses of aldosterone (0.5 microgram.100 g-1.day-1) and dexamethasone (1.2 microgram.100 g-1.day-1) to eliminate changes in adrenal corticosteroid levels. Furosemide was withdrawn 12 h before sodium and potassium transport rates were measured in distal tubules by in vivo microperfusion. We found that increased sodium uptake dramatically enhanced the transport capacity of the distal tubule. Sodium absorption rose from 71.7 to 316.7 pmol.min-1.mm-1, and potassium secretion increased from 30.7 to 73.7 pmol.min-1.mm-1. This response was accompanied by an increase in cell and mitochondrial volume and by proliferation of the basolateral membrane of distal convoluted cells, connecting tubule cells, and principal cells in the distal tubule. We conclude that a chronic increase in sodium uptake by the distal tubule, independent of alterations in extracellular fluid volume and aldosterone levels, stimulates the transport capacity of this nephron segment in part by inducing specific alterations in cell ultrastructure.

Regulation of electrolyte and fluid secretion in salivary acinar cells

1992 ◽  
Vol 263 (6) ◽  
pp. G823-G837 ◽  
Author(s):  
B. Nauntofte
Keyword(s):  
Ionic Composition ◽  
Basolateral Membrane ◽  
Acinar Cells ◽  
Fluid Secretion ◽  
Receptor Activation ◽  
Exchange Mechanism ◽  
Luminal Membrane ◽  
Net Uptake ◽  
Cl Channels ◽  
Simultaneous Activation

The primary secretion from exocrine gland cells is a fluid rich in Na+ and Cl- with a plasmalike ionic composition. Activation of specific receptors on the plasma membrane by hormones and neurotransmitters, which leads to activation of the phosphoinositol metabolism, results in release of Ca2+ from internal Ca2+ stores. Intracellular free Ca2+ concentration ([Ca2+]i) then rises simultaneously at both the basolateral and luminal parts of the acinar cell, reaching maximum values within 1 s after stimulation. In parotid acinar cells, increased [Ca2+]i activates the opening of maxi K+ channels located on the basolateral membrane and Cl- channels presumably located on the luminal membrane, resulting in rapid loss of K+ and Cl- and water and cell shrinkage. Extracellular electroneutrality is maintained by a paracellular Na+ flux into the lumen. Because of the simultaneous activation of K+ and Cl- channels, secretion occurs at a virtually constant membrane potential of about -60 mV. After maximal muscarinic cholinergic stimulation, loss of K+, Cl-, and water results in an approximate 25% reduction in cell volume within 10-15 s after receptor activation. Concomitant with loss of Cl-, there is a loss of HCO3- from the cell, causing a decrease in intracellular pH of 0.1 pH units because of the carbonic anhydrase-mediated conversion of CO2 into H+ and HCO3-. H+ generated from the metabolism and HCO3- production is compensated for by extrusion of H+ by a Na(+)-H+ exchange mechanism, which is responsible for approximately 75% of net Na+ gain that occurs after stimulation. Increased [Na+]i activates the Na(+)-K+ pump, which in turn extrudes Na+ from the cells. In both the unstimulated and stimulated states, cellular production of HCO3- can drive a net uptake of Cl- via the Cl(-)-HCO3- exchange mechanism operating in parallel with the Na(+)-H+ exchanger. The operation of the Cl(-)-HCO3- exchanger is, together with a Na(+)-K(+)-2Cl- cotransport system, essential for maintainance of a high [Cl-]i both in the unstimulated state and during Cl- reuptake.

scholarly journals Retracted: Study Hydrogeochemistry Exploration of Thermal Spring in Ramsar Area, (North of Iran)

2013 ◽  
Vol 2 (11) ◽  
pp. 134-143
Author(s):  
Hamid Reza Samadi
Keyword(s):  
Surface Temperature ◽  
Classification System ◽  
Relevant Information ◽  
Thermal Spring ◽  
Thermal Springs ◽  
High Concentration ◽  
Temperature Conductivity ◽  
Ph Values ◽  
North Of Iran ◽  
Set Up

Ramsar area is located across and between Alborze Mountain and Caspine Sea in North of Iran. About 30 spas are located south of the Ramsar and Sadatshar town. They are almost in between 20 to 70 m elevation. Paleozoic, Mesozoic and Tertiary rocks and alluvial deposit are exposed around the Ramsar area. In tertiary, acidic Plutonism was active and intrusion into the Paleozoic and Cenozoic formations. Quaternary and Alluvium deposits are exposed and extending on the Jurassic formations in Ramsar plain and have thickness lower than 10 m in show springs. The annual precipitation in the Ramsar region is 976 mm. There has not any proper Thermal spring management in Ramsar area yet. This could post some serious problem on improper management of Thermal spring sites, where its environment has been put into jeopardy. This study aims to provide a way to classify the Thermal springs in Ramsar area. The result of this study help in the classification of Thermal spring sites for official planning improvement of administration and sustainable development of natural resources of the area. The study makes use of the Department of Applied Geosciences in Islamic Azad University. GIS data of a total of 9 Thermal springs in the attempt to set up a classification system of Thermal springs in Ramsar area. These data include surface temperature, conductivity, alkalinity, acidity, TDS, pH values, H2S, Ca, Cl, Fe, K, Mg, Mn, Na, SiO2, SO4 contents, their locations, usages and other relevant information. The surface temperature of Thermal springs are between 19oC – 65oC and Na-K-Cageothermometer shows estimated reservoir temperature range from 25 o C – 195 o C. Most of the water from these Thermal springs is relatively turbidness and their composition is sodium choloride. The Thermal springs in this area generally exhibit high SiO2 and Na content; strong smell of sulfur. In addition, there are 9 Thermal springs that show high concentration of Cl, Ca, Na, K and Mg. There are three major criteria used in the classification system in this study, temperature, pH and their usage. On the basis of temperature, there are three classes of Thermal springs in Ramsar area: hyper thermal spring (10 %, 50-99o C); thermal spring (80%, 30-50o C). There is one class achieved on the basis of pH values: all of Thermal springs exhibit weak acids. There are 4 types of usage classification: swimming pools, Tourism, space heating and drying of organic materials.

Hyponatremia in the rat in the absence of positive water balance.

1997 ◽  
Vol 8 (4) ◽  
pp. 524-529
Author(s):  
M Gowrishankar ◽  
C B Chen ◽  
S Cheema-Dhadli ◽  
A Steele ◽  
M L Halperin
Keyword(s):  
Water Balance ◽  
Isotonic Saline ◽  
Extracellular Fluid ◽  
Free Water ◽  
Control Group ◽  
High Concentration ◽  
Additional Control ◽  
Two Factors ◽  
Negative Balance ◽  
Usual Diet

The purpose of this report is to determine the mechanisms that lead to hyponatremia when isotonic saline was the only fluid infused into rats given antidiuretic hormone (ADH), and what might minimize the degree of this hyponatremia. Normal rats were deprived of food and water for the 24-hr study period. They received an infusion of isotonic saline to expand their extracellular fluid (ECF) volume with and without exogenous ADH administration (N = 8 in each of the four groups). Similar studies were also carried out in 32 rats fed a low electrolyte diet for 72 hr before the experiment. An additional control group was fed the low electrolyte diet supplemented with sodium (Na), potassium (K), and chloride (Cl). Hyponatremia developed over 24 hr in rats fed their usual diet if treated with ADH and isotonic saline (fall, 13 +/- 2 mM, P < 0.01). The hyponatremia was caused by negative balance for Na + K salts. Hyponatremia did not develop after the saline + ADH treatment if rats were pretreated for 3 days with a low electrolyte diet. Two factors were required to develop this hyponatremia--generation of electrolyte-free water as a result of the excretion of a large quantity of Na + K salts at a high concentration in the urine, and prevention of the excretion of this electrolyte-free water by ADH. Increasing the avidity for Na reabsorption by the kidney prevented this type of hyponatremia from developing.

Coupled NaCl entry into Necturus gallbladder epithelial cells

1982 ◽  
Vol 243 (3) ◽  
pp. C140-C145 ◽  
Author(s):  
A. C. Ericson ◽  
K. R. Spring
Keyword(s):  
Epithelial Cells ◽  
Cell Volume ◽  
Apical Membrane ◽  
Ionic Composition ◽  
Basolateral Membrane ◽  
Cell Swelling ◽  
Disulfonic Acid ◽  
Bathing Solution ◽  
Parallel Operation ◽  
Solute Content

NaCl entry into Necturus maculosus gallbladder epithelial cells was studied by determination of the rate of fluid movement into the cell when the Na+-K+-ATPase was inhibited by 10(-4) M ouabain in the serosal bathing solution. The cell swelling was due to continuing entrance of NaCl into the cell across the apical membrane, which increased the solute content of the cell; the resultant rise in cell osmolality induced water flow and cell swelling. The rate of swelling was 4.3% of the cell volume per minute, equivalent to a volume flow across the apical membrane of 1.44 x 10(-6) cm/s, similar in magnitude to the normal rate of fluid absorption by the gallbladder. We determined the mechanism of NaCl entry by varying the ionic composition of the mucosal bath; when most of the mucosal Na+ or Cl- was replaced, cell volume did not increase during pump inhibition. The rate of NaCl entry was a saturable function of Na+ or Cl- in the mucosal bathing solution with K1/2 values of 26.6 mM for Na+ and 19.5 mM for Cl-. The mode of NaCl entry was probably not the parallel operation of Na+-H+ and Cl(-)-HCO-3 exchangers because of the lack of effect of bicarbonate removal or of the inhibitors amiloride and 4-acetamido-4'-isothiocyanostilbene-2,2'-disulfonic acid. NaCl entry was reversibly inhibited by bumetanide in the mucosal bathing solution. Transepithelial NaCl and water absorption is the result of the coupled, carrier-mediated movement of NaCl into the cell across the apical membrane and the active extrusion of Na+ by the Na+-K+-ATPase in the basolateral membrane.

Regulation of basolateral K channels in proximal tubule studied during continuous microperfusion

1993 ◽  
Vol 264 (3) ◽  
pp. F496-F501 ◽  
Author(s):  
J. S. Beck ◽  
A. M. Hurst ◽  
J. Y. Lapointe ◽  
R. Laprade
Keyword(s):  
Potassium Channel ◽  
Sodium Transport ◽  
Basolateral Membrane ◽  
Ph Sensitive ◽  
Cell Swelling ◽  
Channel Activity ◽  
Open Probability ◽  
Nephron Segments ◽  
Inwardly Rectifying ◽  
Stimulation Of

Potassium channel activity of the basolateral membrane of the collagenase-treated rabbit proximal convoluted tubule (PCT) was studied during continuous luminal microperfusion. In cell-attached patches (high-K pipette) an inwardly rectifying potassium channel was observed with an inward slope conductance of 60.8 +/- 3.3 pS (n = 12) and outward slope conductance of 17.1 +/- 2.7 pS (n = 6). Stimulation of transcellular sodium transport with luminal glucose and alanine increased channel activity [measured as single-channel open probability (NPo)] from 0.19 +/- 0.11 to 0.44 +/- 0.09 (n = 8). This increase in channel activity was not likely to be mediated by either cell depolarization or cell swelling, because channel activity was voltage insensitive over physiological potentials and because the channel was not activated by stretch. However, channel activity was pH sensitive; reducing luminal pH from 7.4 to 6.5 reduced NPo from 0.63 +/- 0.24 to 0.26 +/- 0.16 (n = 5). Our work demonstrates the feasibility of patch clamping the basolateral membrane of microperfused nephron segments. This has allowed us to follow the activity of this potassium channel during an increase in sodium transport and show that its activity does increase during this maneuver. We conclude that: 1) it is possible to patch clamp the basolateral membrane of microperfused nephron segments, and 2) basolateral membrane of the rabbit PCT contains an inwardly rectifying, pH-sensitive potassium channel. The behavior of this channel on stimulation of transcellular sodium transport could explain the macroscopic increase in basolateral potassium conductance observed under similar conditions.

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