Microsatellite variation associated with prolactin expression and growth of salt-challenged tilapia

2002 ◽  
Vol 9 (1) ◽  
pp. 1-4 ◽  
Author(s):  
J. T. Streelman ◽  
T. D. Kocher
Keyword(s):  
Gene Expression ◽  
Simple Sequence Repeat ◽  
Growth Response ◽  
Regulatory Evolution ◽  
Microsatellite Variation ◽  
Dna Structures ◽  
Microsatellite Alleles ◽  
Affect Gene Expression ◽  
Simple Sequence

Biologists have long argued that runs of alternating purines and pyrimidines could form alternative DNA structures, which might regulate transcription. Here, we report that simple sequence repeat polymorphisms in the tilapia prolactin 1 ( prl 1) promoter are associated with differences in prl 1 gene expression and the growth response of salt-challenged fishes. Individuals homozygous for long microsatellite alleles express less prl 1 in fresh water but more prl 1 in half-seawater than fishes with other genotypes. Our work provides the first in vivo evidence that differences in microsatellite length among individuals may indeed affect gene expression and that variance in expression has concomitant physiological consequences. These results suggest that dinucleotide microsatellites represent an under-appreciated source of genetic variation for regulatory evolution.

Plant simple sequence repeats: distribution, variation, and effects on gene expression

Genome ◽  
2008 ◽  
Vol 51 (2) ◽  
pp. 79-90 ◽  
Author(s):  
Natalya Sharopova
Keyword(s):  
Gene Expression ◽  
Dna Methylation ◽  
Simple Sequence Repeat ◽  
Expression Data ◽  
Sequence Repeat ◽  
Level Control ◽  
Functional Annotations ◽  
Genome Wide ◽  
Affect Gene Expression ◽  
Simple Sequence

Genome-wide simple sequence repeat (SSR) information was analyzed together with functional annotations of Arabidopsis genes and public gene expression data for Arabidopsis and rice. Analysis of more than 15 000 Arabidopsis and more than 16 000 rice SSRs indicated that SSRs may affect the expression of hundreds of genes. Data from experiments on DNA methylation, histone acetylation, and transcript turnover suggest that SSRs may affect gene expression at transcriptional and posttranscriptional levels. Members of some functional groups were shown to be enriched with SSRs and often contained similar but non-homologous repeats within the same gene regions. In addition, the distribution of perfect and imperfect SSRs in some Arabidopsis, maize, and rice genes was used to demonstrate how two-level control of SSR variation may contribute to protein evolution.

scholarly journals Mammalian linker-histone subtypes differentially affect gene expression in vivo

2003 ◽  
Vol 100 (10) ◽  
pp. 5920-5925 ◽  
Author(s):  
R. Alami ◽  
Y. Fan ◽  
S. Pack ◽  
T. M. Sonbuchner ◽  
A. Besse ◽  
...  
Keyword(s):  
Gene Expression ◽  
Linker Histone ◽  
Affect Gene Expression

scholarly journals Helicases FANCJ, RTEL1 and BLM Act on Guanine Quadruplex DNA in Vivo

Genes ◽  
2019 ◽  
Vol 10 (11) ◽  
pp. 870 ◽  
Author(s):  
Peter Lansdorp ◽  
Niek van Wietmarschen
Keyword(s):  
Gene Expression ◽  
Genome Stability ◽  
Cell Function ◽  
Historical Context ◽  
Telomere Maintenance ◽  
Dna Structures ◽  
G4 Dna ◽  
Guanine Quadruplex

Guanine quadruplex (G4) structures are among the most stable secondary DNA structures that can form in vitro, and evidence for their existence in vivo has been steadily accumulating. Originally described mainly for their deleterious effects on genome stability, more recent research has focused on (potential) functions of G4 structures in telomere maintenance, gene expression, and other cellular processes. The combined research on G4 structures has revealed that properly regulating G4 DNA structures in cells is important to prevent genome instability and disruption of normal cell function. In this short review we provide some background and historical context of our work resulting in the identification of FANCJ, RTEL1 and BLM as helicases that act on G4 structures in vivo. Taken together these studies highlight important roles of different G4 DNA structures and specific G4 helicases at selected genomic locations and telomeres in regulating gene expression and maintaining genome stability.

scholarly journals In Vivo Treatments with Fulvestrant and Anastrozole Differentially Affect Gene Expression in the Rat Efferent Ductules1

2011 ◽  
Vol 84 (1) ◽  
pp. 52-61 ◽  
Author(s):  
Gisele Renata Oliveira Gomes ◽  
Fabiana Yasuhara ◽  
Erica Rosanna Siu ◽  
Sheilla Alessandra Ferreira Fernandes ◽  
Maria Christina Werneck Avellar ◽  
...  
Keyword(s):  
Gene Expression ◽  
Affect Gene Expression

scholarly journals Hypothesis: Regulation of neuroplasticity may involve I-motif and G-quadruplex DNA formation modulated by epigenetic mechanisms

2018 ◽  
Author(s):  
German I. Todorov ◽  
Catarina Cunha
Keyword(s):  
Gene Expression ◽  
Synaptic Plasticity ◽  
Epigenetic Mechanisms ◽  
Rna Structures ◽  
Regulatory Activity ◽  
Dna Structures ◽  
Dna And Rna ◽  
Neuronal Mechanisms ◽  
G Quadruplex

Recent studies demonstrated the existence in vivo of various functional DNA structures that differ from the double helix. The G-quadruplex (G4) and intercalated motif (I-motif or IM) DNA structures are formed as knots where, correspondingly, guanines or cytosines on the same strand of DNA bind to each other. There are grounds to believe that G4 and IM sequences play a significant role in regulating gene expression considering their tendency to be found in or near regulatory sites (such as promoters, enhancers, and telomeres) as well as the correlation between the prevalence of G4 or IM conformations and specific phases of cell cycle. Notably, G4 and IM capable sequences tend to be found on the opposite strands of the same DNA site with at most one of the two structures formed at any given time. The recent evidence that K+, Mg2+ concentrations directly affect IM formation (and likely G4 formation indirectly) lead us to believe that these structures may play a major role in synaptic plasticity of neurons, and, therefore, in a variety of central nervous system (CNS) functions including memory, learning, habitual behaviors, pain perception and others. Furthermore, epigenetic mechanisms, which have an important role in synaptic plasticity and memory formation, were also shown to influence formation and stability of G4s and IMs. Our hypothesis is that non-canonical DNA and RNA structures could be an integral part of neuroplasticity control via gene expression regulation at the level of transcription, translation and splicing. We propose that the regulatory activity of DNA IM and G4 structures is modulated by DNA methylation/demethylation of the IM and/or G4 sequences, which facilitates the switch between canonical and non-canonical conformation. Other neuronal mechanisms interacting with the formation and regulatory activity of non-canonical DNA and RNA structures, particularly G4, IM and triplexes, may involve microRNAs as well as ion and proton fluxes. We are proposing experiments in acute brain slices and in vivo to test our hypothesis. The proposed studies would provide new insights into fundamental neuronal mechanisms in health and disease and potentially open new avenues for treating mental health disorders.

Acute intrarenal infusion of ANG II does not stimulate immediate early gene expression in the kidney

2002 ◽  
Vol 282 (4) ◽  
pp. R1133-R1139 ◽  
Author(s):  
Amanda J. Edgley ◽  
Nancy R. Nichols ◽  
Warwick P. Anderson
Keyword(s):  
Gene Expression ◽  
Growth Response ◽  
Early Growth ◽  
Early Gene ◽  
Immediate Early ◽  
Vehicle Group ◽  
Ang Ii ◽  
Early Growth Response ◽  
Renal Expression

ANG II is capable of stimulating expression of immediate early genes such as egr-1 and c- fos in a variety of cultured cells, including cells of renal origin. To investigate whether ANG II can stimulate early growth response gene expression in vivo, we studied the effects of acute renal artery infusion of low-dose ANG II (2.5 ng · kg−1 · min−1) or vehicle on the renal expression of c- fos and egr-1 genes in rats. ANG II infusion for 30 or 240 min decreased renal vascular conductance by ∼13 and 8%, respectively, compared with the vehicle group. Expression of the early growth response genes c-fosand egr-1 was analyzed using Northern blot hybridization. No significant upregulation of c- fos or egr-1 mRNA levels was detected in rats that received ANG II for either 30 or 240 min, compared with the vehicle groups. We conclude that ANG II, at doses that cause significant physiological effects, does not increase the renal expression of c- fos or egr-1 genes over periods of up to 4 h in vivo.

Do the microRNAs we eat affect gene expression?

Nature ◽  
2020 ◽  
Vol 582 (7812) ◽  
pp. S10-S11 ◽  
Author(s):  
Kristina Campbell
Keyword(s):  
Gene Expression ◽  
Affect Gene Expression

Effect of X-irradiation on gene expression of rat liver chemokines: in vivo and in vitro studies

2008 ◽  
Vol 46 (01) ◽  
Author(s):  
F Moriconi ◽  
H Christiansen ◽  
H Christiansen ◽  
N Sheikh ◽  
J Dudas ◽  
...  
Keyword(s):  
Gene Expression ◽  
Rat Liver ◽  
In Vitro Studies ◽  
X Irradiation
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