scholarly journals Gene expression tomography

2002 ◽  
Vol 8 (2) ◽  
pp. 159-167 ◽  
Author(s):  
VANESSA M. BROWN ◽  
ALEX OSSADTCHI ◽  
ARSHAD H. KHAN ◽  
SANJIV S. GAMBHIR ◽  
SIMON R. CHERRY ◽  
...  
Keyword(s):  
Gene Expression ◽  
Image Reconstruction ◽  
Expression Analysis ◽  
Gene Expression Analysis ◽  
Expression Patterns ◽  
Brain Slices ◽  
Axial Rotation ◽  
Gene Expression Patterns ◽  
Rnase Protection ◽  
The Brain

Gene expression tomography, or GET, is a new method to increase the speed of three-dimensional (3-D) gene expression analysis in the brain. The name is evocative of the method’s dual foundations in high-throughput gene expression analysis and computerized tomographic image reconstruction, familiar from techniques such as positron emission tomography (PET) and X-ray computerized tomography (CT). In GET, brain slices are taken using a cryostat in conjunction with axial rotation about independent axes to create a series of “views” of the brain. Gene expression information obtained from the axially rotated views can then be used to recreate 3-D gene expression patterns. GET was used to successfully reconstruct images of tyrosine hydroxylase gene expression in the mouse brain, using both RNase protection and real-time quantitative reverse transcription PCR (QRT-PCR). A Monte-Carlo analysis confirmed the good quality of the GET image reconstruction. By speeding acquisition of gene expression patterns, GET may help improve our understanding of the genomics of the brain in both health and disease.

scholarly journals Comparative uterine gene expression analysis after dioxin and estradiol administration

2004 ◽  
Vol 33 (3) ◽  
pp. 763-771 ◽  
Author(s):  
H Watanabe ◽  
A Suzuki ◽  
M Goto ◽  
S Ohsako ◽  
C Tohyama ◽  
...  
Keyword(s):  
Gene Expression ◽  
Expression Analysis ◽  
Gene Expression Analysis ◽  
Estrogenic Activity ◽  
Expression Patterns ◽  
Environmental Pollutant ◽  
Abnormal Development ◽  
Gene Expression Patterns ◽  
Endocrine Effects ◽  
Antiestrogenic Activity

The environmental pollutant 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) adversely affects many organisms. TCDD exposure is known to be associated with abnormal development, hepatotoxicity and endocrine effects. It has also been reported to have antiestrogenic activity in addition to estrogenic activity. In order to clarify the effects of TCDD in the uterus, we evaluated the patterns of gene expression after TCDD and estradiol administration. Of the 10 000 arrayed genes, only a few were affected by both estradiol and TCDD. Although the subset of genes that responded to estrogen was also activated by TCDD, the response to TCDD was more limited than that observed in response to estradiol. Therefore, according to our analysis of gene expression patterns, TCDD had partial and weak estrogenic activity in the uterus.

scholarly journals Gene expression analysis characterizes antemortem stress and has implications for establishing cause of death

2011 ◽  
Vol 43 (16) ◽  
pp. 974-980 ◽  
Author(s):  
David Jardine ◽  
Leanne Cornel ◽  
Mary Emond
Keyword(s):  
Gene Expression ◽  
Cause Of Death ◽  
Expression Analysis ◽  
Liver Tissue ◽  
Gene Expression Analysis ◽  
Expression Patterns ◽  
Gene Expression Patterns ◽  
Sudden Infant ◽  
K Nearest Neighbor ◽  
Diagnostic Laboratory

Within the field of forensic pathology, determination of the cause of death depends upon identifying physical changes in the corpse or finding diagnostic laboratory abnormalities. When such perturbations are absent, definitive assignment of a cause of death may be difficult or impossible. An example of such a problem is sudden infant death syndrome (SIDS), a common cause of neonatal mortality that does not produce physical findings or laboratory abnormalities. Although respiratory failure as a cause of SIDS represents the most widely held hypothesis, sudden cardiac death and hyperthermia have also been advanced as possible causes. We hypothesize that each of these physiological stresses would produce a different pattern of premortem gene expression and that these patterns of gene expression would remain evident in tissues collected postmortem. If these patterns were sufficiently distinctive, they could be used to identify the cause of death. Using an infant mouse model, we compared gene expression patterns in liver tissue after sudden death, lethal hyperthermia, and lethal hypoxia. Each of these conditions produced readily distinguishable differences in gene expression patterns. With the K-nearest neighbor classification algorithm, only 10 genes are necessary to correctly classify samples. If the liver tissue was not harvested immediately after death, additional alteration in gene expression patterns resulted; however, these alterations did not affect the group of genes used to classify the samples. Our findings suggest that gene expression analysis from tissues collected postmortem may provide useful clues about certain physiologic stresses that may precede death.

Laser capture microdissection protocol for gene expression analysis in the brain

2017 ◽  
Vol 148 (3) ◽  
pp. 299-311 ◽  
Author(s):  
P. Garrido-Gil ◽  
P. Fernandez-Rodríguez ◽  
J. Rodríguez-Pallares ◽  
Jose L. Labandeira-Garcia
Keyword(s):  
Gene Expression ◽  
Expression Analysis ◽  
Laser Capture Microdissection ◽  
Gene Expression Analysis ◽  
Laser Capture ◽  
The Brain
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