Left ventricle transcriptomic analysis reveals connective tissue accumulation associates with initial age-dependent decline in V̇o2peak from its lifetime apex

2017 ◽  
Vol 49 (1) ◽  
pp. 53-66 ◽  
Author(s):  
Gregory N. Ruegsegger ◽  
Ryan G. Toedebusch ◽  
Joshua F. Braselton ◽  
Thomas E. Childs ◽  
Frank W. Booth
Keyword(s):  
Connective Tissue ◽  
Left Ventricle ◽  
Wheel Running ◽  
Protein Translation ◽  
Transcriptomic Analysis ◽  
Peak Oxygen Consumption ◽  
Activity Levels ◽  
Tissue Development ◽  
Female Wistar Rats ◽  
Better Than

Peak oxygen consumption (V̇o2peak) strongly predicts morbidity and mortality better than other established risk factors, yet mechanisms associated with its age-associated decline are unknown. Our laboratory has shown that V̇o2peak first begins to decrease at the same age of 19–20 wk in both sedentary and wheel-running, female Wistar rats (Toedebusch et al., Physiol Genomics. 48: 101–115, 2016). Here, we employed a total systemic approach using unsupervised interrogation of mRNA with RNA sequencing. The purpose of our study was to analyze transcriptomic profiles from both sedentary (SED) and wheel-running (RUN) conditions as a strategy to identify pathways in the left ventricle that may contribute to the initial reductions in V̇o2peak occurring between 19 and 27 wk of age. Transcriptomic comparisons were made within both SED and RUN rats between 19 and 27 wk ( n = 5–8). Analysis of mRNAs shared in SED and RUN between 19 and 27 wk found 17 upregulated (e.g., Adra1d, Rpl17, Xpo7) and 8 downregulated (e.g., Cdo1, Ctfg, Sfrp1) mRNAs, at 19 wk, respectively. Furthermore, bioinformatics analysis of mRNAs common to SED and RUN produced networks suggestive of increased connective tissue development at 27 vs. 19 wk. Additionally, Ctfg mRNA was negatively associated with V̇o2peak in both SED and RUN ( P < 0.05). In summary, transcriptomic analysis revealed mRNAs and networks associated with increased connective tissue development, decreased α-adrenergic activity, and decreased protein translation in the left ventricle that could, in part, potentially influence the initiation of the lifelong reduction in V̇o2peak, independent of physical activity levels.

scholarly journals I. A contribution to the anatomy of the ductless glands and lymphatic system of the angler fish ( Lophius piscatorius )

1927 ◽  
Vol 215 (421-430) ◽  
pp. 1-56 ◽  
Keyword(s):  
Connective Tissue ◽  
Lymphatic System ◽  
Loose Connective Tissue ◽  
Mode Of Life ◽  
Better Than ◽  
Considerable Size

The Angler ( Lophius piscatorius ) is a fish much modified for a bottom habit, and apart from many peculiarities of form and structure associated with this particular mode of life, is remarkable for the looseness of its skin and the abundance of soft connective tissue that separates it from the underlying fascia and muscles. Within this layer of loose connective tissue lie many of the larger trunks of the lymphatic system, mostly of very considerable size and easy to inject. The fish thus furnishes material better than most for the study of this system.

scholarly journals Primary Cardiac Fibroma in an Adult

2015 ◽  
Vol 2015 ◽  
pp. 1-3
Author(s):  
Samuel H. Cho ◽  
Timothy Fritz ◽  
Lynn J. Cronin ◽  
Stephen D. Cohle
Keyword(s):  
Connective Tissue ◽  
Left Ventricle ◽  
Pediatric Population ◽  
Adult Population ◽  
Surgical Excision ◽  
Imaging Modalities ◽  
Cardiac Fibroma ◽  
Primary Tumors ◽  
Immunohistological Analysis ◽  
Unusual Finding

Cardiac fibromas are benign primary tumors composed of connective tissue and fibroblasts. These uncommon tumors are primarily found in the pediatric population, and their prevalence among the adult population is exceedingly rare. We report a case of an adult with nonspecific symptoms, who was subsequently found to have a solitary mass located in the left ventricle. This case highlights an unusual finding in an adult who through various imaging modalities, surgical excision, and immunohistological analysis was found to have a cardiac fibroma.

scholarly journals Can myocardial work indices contribute to the exploration of patients with cardiac amyloidosis?

Open Heart ◽  
2020 ◽  
Vol 7 (2) ◽  
pp. e001346
Author(s):  
Aénora Roger-Rollé ◽  
Eve Cariou ◽  
Khailène Rguez ◽  
Pauline Fournier ◽  
Yoan Lavie-Badie ◽  
...  
Keyword(s):  
Cardiac Amyloidosis ◽  
Characteristic Curve ◽  
Global Longitudinal Strain ◽  
Left Ventricular ◽  
Peak Oxygen Consumption ◽  
Left Ventricular Ejection ◽  
Ventricular Ejection Fraction ◽  
Echocardiographic Parameters ◽  
Global Work ◽  
Better Than

BackgroundCardiac amyloidosis (CA) is a life-threatening restrictive cardiomyopathy. Identifying patients with a poor prognosis is essential to ensure appropriate care. The aim of this study was to compare myocardial work (MW) indices with standard echocardiographic parameters in predicting mortality among patients with CA.MethodsClinical, biological and transthoracic echocardiographic parameters were retrospectively compared among 118 patients with CA. Global work index (GWI) was calculated as the area of left ventricular pressure–strain loop. Global work efficiency (GWE) was defined as percentage ratio of constructive work to sum of constructive and wasted works. Sixty-one (52%) patients performed a cardiopulmonary exercise.ResultsGWI, GWE, global longitudinal strain (GLS), left ventricular ejection fraction (LVEF) and myocardial contraction fraction (MCF) were correlated with N-terminal prohormone brain natriuretic peptide (R=−0.518, R=−0.383, R=−0.553, R=−0.382 and R=−0.336, respectively; p<0.001). GWI and GLS were correlated with peak oxygen consumption (R=0.359 and R=0.313, respectively; p<0.05). Twenty-eight (24%) patients died during a median follow-up of 11 (4–19) months. The best cut-off values to predict all-cause mortality for GWI, GWE, GLS, LVEF and MCF were 937 mm Hg/%, 89%, 10%, 52% and 15%, respectively. The area under the receiver operator characteristic curve of GWE, GLS, GWI, LVEF and MCF were 0.689, 0.631, 0.626, 0.511 and 0.504, respectively.ConclusionIn CA population, MW indices are well correlated with known prognosis markers and are better than LVEF and MCF in predicting mortality. However, MW does not perform better than GLS.

scholarly journals Improvement of Skeletal Muscle Regeneration by Platelet-Rich Plasma in Rats with Experimental Chronic Hyperglycemia

2020 ◽  
Vol 2020 ◽  
pp. 1-9
Author(s):  
Raed Rtail ◽  
Olena Maksymova ◽  
Viacheslav Illiashenko ◽  
Olena Gortynska ◽  
Oleksii Korenkov ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Connective Tissue ◽  
Muscle Regeneration ◽  
Platelet Rich Plasma ◽  
Triceps Surae ◽  
Skeletal Muscle Regeneration ◽  
Male Rats ◽  
Tissue Development ◽  
Muscle Recovery ◽  
Chronic Hyperglycemia

Herein, the structural effect of autologous platelet-rich plasma (PRP) on posttraumatic skeletal muscle regeneration in rats with chronic hyperglycemia (CH) was tested. 130 white laboratory male rats divided into four groups (I—control; II—rats with CH; III—rats with CH and PRP treatment; and IV—rats for CH confirmation) were used for the experiment. CH was simulated by streptozotocin and nicotinic acid administration. Triceps surae muscle injury was reproduced by transverse linear incision. Autologous PRP was used in order to correct the possible negative CH effect on skeletal muscle recovery. On the 28th day after the injury, the regenerating muscle fiber and blood vessel number in the CH+PRP group were higher than those in the CH rats. However, the connective tissue area in the CH group was larger than that in the CH+PRP animals. The amount of agranulocytes in the regenerating muscle of the CH rats was lower compared to that of the CH+PRP group. The histological analysis of skeletal muscle recovery in CH+PRP animals revealed more intensive neoangiogenesis compared to that in the CH group. Herewith, the massive connective tissue development and inflammation signs were observed within the skeletal muscle of CH rats. Obtained results suggest that streptozotocin-induced CH has a negative effect on posttraumatic skeletal muscle regeneration, contributing to massive connective tissue development. The autologous PRP injection promotes muscle recovery process in rats with CH, shifting it away from fibrosis toward the complete muscular organ repair.

scholarly journals A method for isolating RNA from canine bone

BioTechniques ◽  
2020 ◽  
Vol 68 (6) ◽  
pp. 311-317
Author(s):  
Rebecca Nance ◽  
Payal Agarwal ◽  
Maninder Sandey ◽  
Dmytro Starenki ◽  
Jey Koehler ◽  
...  
Keyword(s):  
Gene Expression ◽  
Bone Marrow ◽  
Connective Tissue ◽  
Bone Cells ◽  
Transcriptomic Analysis ◽  
Unique Challenge ◽  
Downstream Application ◽  
Transcriptomic Sequencing ◽  
Mineralized Matrix

Extracting sufficient quantity and quality RNA from bone is essential for downstream application, such as transcriptomic sequencing, to evaluate gene expression. Isolation of RNA from bone presents a unique challenge owing to the hypocellular, brittle and mineralized matrix, which makes homogenizing the tissue difficult and provides little RNA to work with. Removal of contaminating tissue, such as bone marrow and connective tissue, is essential for isolating RNA that is unique to osteoblasts, osteoclasts and osteocytes. This study established a method to effectively isolate RNA from normal canine bone cells using the phalanges, without contamination from other tissue types, for downstream transcriptomic analysis.

scholarly journals Phyllosphere-inhabiting endophytic bacteria feature a stationary phase-like lifestyle

2021 ◽  
Author(s):  
André C Velásquez ◽  
José C Huguet-Tapia ◽  
Sheng Yang He
Keyword(s):  
Stationary Phase ◽  
Pseudomonas Syringae ◽  
Stress Responses ◽  
Endophytic Bacteria ◽  
Physiological State ◽  
Protein Translation ◽  
Transcriptomic Analysis ◽  
In Planta ◽  
Population Densities

Plants are in constant association with a variety of microbes, and although much is known about how symbiotic and pathogenic microbes interact with plants, less is known about the population dynamics, adaptive traits, and transcriptional features of endophytic commensal microbes that live inside leaves. In this study, we evaluated the long-term population and transcriptional dynamics of two bacterial microbiota endophytes and compared them to those of a commensal-simulating non-pathogenic mutant of the bacterial pathogen Pseudomonas syringae. We found that population densities of all three endophytic phyllosphere bacteria remained static over a long period of time, which was caused by a continual equilibrium between bacterial multiplication and death, as evidenced by treatment of plants with antibiotics that only targeted dividing bacteria or by in planta visualization of bacteria carrying a fluorescent division reporter. Population stasis could not be explained by a lack of resources, as Arabidopsis leaves could support population densities up to 100 times higher than the normal microbiota populations, nor was population stasis reversed by significantly quenching PAMP-triggered immunity. Long-term temporal in planta transcriptomic analysis of these three bacterial endophytes revealed up-regulation of protein translation, the generation of energy, and the response to stress, and interestingly, for the microbiota strains, the longer the bacteria remained inside plants, the greater the up-regulation of some of these processes. Further transcriptomic analysis of in planta populations of commensal-simulating Pseudomonas syringae revealed a remarkable resemblance to those of in vitro bacteria in stationary phase, a metabolically active physiological state in which the production of secondary metabolites and stress responses are induced. This study provides novel insight into how endophytic bacteria survive and thrive inside plant leaves, and reshapes our current understanding of what it means to be part of the endophytic microbiota in the phyllosphere.

scholarly journals Assessment of subclinical hypertention-mediated heart damage in young adults with connective tissue dysplasia

2021 ◽  
Author(s):  
М.И. Шупина ◽  
Ю.В. Терещенко ◽  
В.В. Потапов ◽  
Г.И. Нечаева
Keyword(s):  
Young Adults ◽  
Connective Tissue ◽  
Left Ventricle ◽  
Interventricular Septum ◽  
Left Ventricle Mass ◽  
Hypertensive Patients ◽  
Heart Damage ◽  
Upper Limit ◽  
Echocardiographic Examination ◽  
Connective Tissue Dysplasia

С целью оценки особенностей опосредованного артериальной гипертензией (АГ) поражения сердца у лиц молодого возраста с АГ и дисплазией соединительной ткани (ДСТ) проведено эхокардиографическое обследование 574 человек в возрасте от 18 до 29 лет. При анализе полученных данных обнаружено, что у молодых людей с АГ и ДСТ толщина межжелудочковой перегородки, стенок левого желудочка, массы и индексированных показателей массы левого желудочка не достигают общепризнанной единой верхней границы нормы. Это ведет к систематической гиподиагностике поражения сердца как органа-мишени, некорректной оценке стадии АГ и неадекватной тактике ведения этих пациентов. In order to assess the features of hypertension-mediated heart damage in young adults with hypertension and connective tissue dysplasia (СTD), an echocardiographic examination of 574 people aged 18 to 29 years was performed. It was revealed that young adults with hypertension and CTD have lower levels of the interventricular septum and left ventricle walls thickness, left ventricle mass and its indexes than in hypertensive patients without CTD, and these parameters do not reach the generally recognized upper limit of the norm. This leads to systematic underdiagnosis of hypertension-mediated heart damage, incorrect assessment of the stage of hypertension, and inadequate management for these patients.

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