PKA, Germ Cells, and Fertility

Physiology ◽  
2007 ◽  
Vol 22 (1) ◽  
pp. 40-46 ◽  
Author(s):  
Kimberly A. Burton ◽  
G. Stanley McKnight
Keyword(s):  
Sperm Motility ◽  
Germ Cells ◽  
Genetic Mutations ◽  
Spatial Regulation ◽  
Temporal And Spatial

Temporal and spatial regulation of PKA activity are essential for vigorous sperm motility and for the resumption of meiosis in oocytes, two events required for successful fertilization. Genetic mutations in mice that affect PKA signaling in germ cells lead to infertility and illustrate the importance of this pathway in mammalian reproduction.

scholarly journals Distinct roles of resident and nonresident macrophages in nonischemic cardiomyopathy

2018 ◽  
Vol 115 (20) ◽  
pp. E4661-E4669 ◽  
Author(s):  
Xudong Liao ◽  
Yuyan Shen ◽  
Rongli Zhang ◽  
Keiki Sugi ◽  
Neelakantan T. Vasudevan ◽  
...  
Keyword(s):  
Heart Failure ◽  
Cardiac Function ◽  
Late Phase ◽  
Myeloid Cells ◽  
Pressure Overload ◽  
Genetic Mutations ◽  
Nonischemic Cardiomyopathy ◽  
Resident Macrophage ◽  
Temporal And Spatial

Nonischemic cardiomyopathy (NICM) resulting from long-standing hypertension, valvular disease, and genetic mutations is a major cause of heart failure worldwide. Recent observations suggest that myeloid cells can impact cardiac function, but the role of tissue-intrinsic vs. tissue-extrinsic myeloid cells in NICM remains poorly understood. Here, we show that cardiac resident macrophage proliferation occurs within the first week following pressure overload hypertrophy (POH; a model of heart failure) and is requisite for the heart’s adaptive response. Mechanistically, we identify Kruppel-like factor 4 (KLF4) as a key transcription factor that regulates cardiac resident macrophage proliferation and angiogenic activities. Finally, we show that blood-borne macrophages recruited in late-phase POH are detrimental, and that blockade of their infiltration improves myocardial angiogenesis and preserves cardiac function. These observations demonstrate previously unappreciated temporal and spatial roles for resident and nonresident macrophages in the development of heart failure.

scholarly journals Temporal and spatial regulation of protein cross-linking by the pre-assembled substrates of a Bacillus subtilis spore coat transglutaminase

PLoS Genetics ◽  
2019 ◽  
Vol 15 (4) ◽  
pp. e1007912 ◽  
Author(s):  
Catarina G. Fernandes ◽  
Diogo Martins ◽  
Guillem Hernandez ◽  
Ana L. Sousa ◽  
Carolina Freitas ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Cross Linking ◽  
Spore Coat ◽  
Subtilis Spore ◽  
Spatial Regulation ◽  
Bacillus Subtilis Spore ◽  
Temporal And Spatial ◽  
Protein Cross Linking

scholarly journals Temporal and spatial regulation of a gene expressed during rat heart maturation • 123

1997 ◽  
Vol 41 ◽  
pp. 23-23
Author(s):  
Thomas J L'Ecuyer ◽  
Kimberly A Mendel ◽  
Allen D Everett
Keyword(s):  
Rat Heart ◽  
Spatial Regulation ◽  
Temporal And Spatial

Use of Green Fluorescent Protein (GFT)-Chimeras to Study Cytoskeletal Assembly and Dynamics

1998 ◽  
Vol 4 (S2) ◽  
pp. 1010-1011
Author(s):  
G. G. Gundersen ◽  
A. Mikhailov ◽  
J. L. Martys ◽  
L. Ho ◽  
R. K. H. Liem ◽  
...  
Keyword(s):  
Green Fluorescent Protein ◽  
Cell Lines ◽  
Intermediate Filaments ◽  
Fluorescent Protein ◽  
Cell Structure ◽  
Β1 Integrin ◽  
Spatial Regulation ◽  
Temporal And Spatial ◽  
Green Fluorescent ◽  
3T3 Cell Lines

The cytoskeleton plays an important role in cell structure, polarity, locomotion and division. Individual elements of the cytoskeleton are composed of subunit proteins which assemble and disassemble in specific places and times within the cell. Knowledge of the temporal and spatial regulation of subunit assembly and disassembly is essential to understanding how the cytoskeleton contributes to cellular activities. The assembly and dynamics of two cytoskeletal structures, namely adhesion plaques (APs) and intermediate filaments (IFs), have been difficult to study by traditional methods. We have generated GFP-chimeras to label these structures and to study their dynamics in motile fibroblasts.To study the dynamics of APs, we prepared stable 3T3 cell lines expressing a GFP-β1 integrin chimera. The chimera was prepared by fusing GFP to the transmembrane and cytoplasmic portions of β1 intergrin, since previous studies had shown that the cytoplasmic tail of β integrins is sufficient to direct integrins to APs.

scholarly journals Temporal and Spatial Regulation of Chemotaxis

2002 ◽  
Vol 3 (4) ◽  
pp. 469-478 ◽  
Author(s):  
Miho Iijima ◽  
Yi Elaine Huang ◽  
Peter Devreotes
Keyword(s):  
Spatial Regulation ◽  
Temporal And Spatial
Sign in / Sign up

Export Citation Format

Share Document