scholarly journals Increased Na+ and K+ currents in small mouse dorsal root ganglion neurons after ganglion compression

2011 ◽  
Vol 106 (1) ◽  
pp. 211-218 ◽  
Author(s):  
Ni Fan ◽  
Parul Sikand ◽  
David F. Donnelly ◽  
Chao Ma ◽  
Robert H. LaMotte
Keyword(s):  
Dorsal Root Ganglion ◽  
Dorsal Root ◽  
Neuronal Response ◽  
Small Diameter ◽  
Neuronal Cell ◽  
Radicular Pain ◽  
Dorsal Root Ganglion Neurons ◽  
Delayed Rectifier ◽  
Ganglion Neurons

We investigated the effects of chronic compression (CCD) of the L3 and L4 dorsal root ganglion (DRG) on pain behavior in the mouse and on the electrophysiological properties of the small-diameter neuronal cell bodies in the intact ganglion. CCD is a model of human radicular pain produced by intraforaminal stenosis and other disorders affecting the DRG, spinal nerve, or root. On days 1, 3, 5, and 7 after the onset of compression, there was a significant decrease from preoperative values in the threshold mechanical force required to elicit a withdrawal of the foot ipsilateral to the CCD (tactile allodynia). Whole cell patch-clamp recordings were obtained, in vitro, from small-sized somata and, for the first time, in the intact DRG. Under current clamp, CCD neurons exhibited a significantly lower rheobase compared with controls. A few CCD but no control neurons exhibited spontaneous action potentials. CCD neurons showed an increase in the density of TTX-resistant and TTX-sensitive Na+ current. CCD neurons also exhibited an enhanced density of voltage-dependent K+ current, due to an increase in delayed rectifier K+ current, without a change in the transient or “A” current. We conclude that CCD in the mouse produces a model of radicular pain, as we have previously demonstrated in the rat. While the role of enhanced K+ current remains to be elucidated, we speculate that it represents a compensatory neuronal response to reduce ectopic or aberrant levels of neuronal activity produced by the injury.

scholarly journals Chronic compression of mouse dorsal root ganglion alters voltage-gated sodium and potassium currents in medium-sized dorsal root ganglion neurons

2011 ◽  
Vol 106 (6) ◽  
pp. 3067-3072 ◽  
Author(s):  
Ni Fan ◽  
David F. Donnelly ◽  
Robert H. LaMotte
Keyword(s):  
Dorsal Root Ganglion ◽  
Dorsal Root ◽  
Small Diameter ◽  
Radicular Pain ◽  
Dorsal Root Ganglion Neurons ◽  
Delayed Rectifier ◽  
Drg Neurons ◽  
Voltage Dependent ◽  
Ganglion Neurons

Chronic compression (CCD) of the dorsal root ganglion (DRG) is a model of human radicular pain produced by intraforaminal stenosis and other disorders affecting the DRG, spinal nerve, or root. Previously, we examined electrophysiological changes in small-diameter lumbar level 3 (L3) and L4 DRG neurons treated with CCD; the present study extends these observations to medium-sized DRG neurons, which mediate additional sensory modalities, both nociceptive and non-nociceptive. Whole-cell patch-clamp recordings were obtained from medium-sized somata in the intact DRG in vitro. Compared with neurons from unoperated control animals, CCD neurons exhibited a decrease in the current threshold for action potential generation. In the CCD group, current densities of TTX-resistant and TTX-sensitive Na+ current were increased, whereas the density of delayed rectifier voltage-dependent K+ current was decreased. No change was observed in the transient or “A” current after CCD. We conclude that CCD in the mouse produces hyperexcitability in medium-sized DRG neurons, and the hyperexcitability is associated with an increased density of Na+ current and a decreased density of delayed rectifier voltage-dependent K+ current.

Antioxidants are necessary for myelination of dorsal root ganglion neurons, in vitro

Glia ◽  
2003 ◽  
Vol 45 (1) ◽  
pp. 54-58 ◽  
Author(s):  
Jewel L. Podratz ◽  
Esther H. Rodriguez ◽  
Anthony J. Windebank
Keyword(s):  
Dorsal Root Ganglion ◽  
Dorsal Root ◽  
Dorsal Root Ganglion Neurons ◽  
Ganglion Neurons

Variation in IH, IIR, and ILEAK between acutely isolated adult rat dorsal root ganglion neurons of different size

1994 ◽  
Vol 71 (1) ◽  
pp. 271-279 ◽  
Author(s):  
R. S. Scroggs ◽  
S. M. Todorovic ◽  
E. G. Anderson ◽  
A. P. Fox
Keyword(s):  
Dorsal Root Ganglion ◽  
Action Potentials ◽  
Dorsal Root ◽  
Membrane Surface ◽  
Small Diameter ◽  
Reversal Potential ◽  
Dorsal Root Ganglion Neurons ◽  
Time Dependent ◽  
Drg Neurons ◽  
Ganglion Neurons

1. The distribution of IH, IIR, and ILEAK was studied in different diameter rat dorsal root ganglion (DRG) neuron cell bodies (neurons). DRG neurons were studied in three diameter ranges: small (19–27 microns), medium (33–37 microns), and large (44-54 microns). IH was defined as a slowly activating inward current evoked by hyperpolarizing voltage steps from a holding potential (HP) of -60 mV, and blocked by 1 mM Cs2+ but not 1 mM Ba2+. Inward rectifier current (IIR) was defined as a rapidly activating current evoked by hyperpolarizations from HP -60 mV, which rectified inwardly around the reversal potential for potassium (EK), and was completely blocked by 100 microM Ba2+. ILEAK was defined as an outward resting current at HP -60 mV, which did not rectify and was blocked by 100 microM Ba2+ but not by 2 mM Cs+. 2. IH was observed in 23 of 23 large, 11 of 12 medium, and in 9 of 20 small diameter DRG neurons tested. Peak IH normalized to membrane surface area was significantly greater in large than in medium or small diameter DRG neurons expressing IH. All neurons exhibiting IH under voltage clamp conditions had short duration action potentials and exhibited time-dependent rectification under current clamp conditions, properties similar to A-type DRG neurons. The 11 small diameter neurons not expressing IH had long duration action potentials and did not exhibit time-dependent rectification, properties similar to C-type DRG neurons. 3. IIR was detected in 18 of 22 medium diameter neurons tested.(ABSTRACT TRUNCATED AT 250 WORDS)

scholarly journals 3D in vitro peripheral nervous system organoid using mouse primary dorsal root ganglion neurons and Schwann cells

IBRO Reports ◽  
2019 ◽  
Vol 6 ◽  
pp. S124
Author(s):  
Woon-Hae Kim ◽  
Hyun-Gyu Kang ◽  
Taehoon H. Kim ◽  
Yoon Jeong Mo ◽  
Yu Seon Kim ◽  
...  
Keyword(s):  
Nervous System ◽  
Dorsal Root Ganglion ◽  
Peripheral Nervous System ◽  
Schwann Cells ◽  
Dorsal Root ◽  
Dorsal Root Ganglion Neurons ◽  
Ganglion Neurons

scholarly journals Noradrenaline regulates substance P release from rat dorsal root ganglion neurons in vitro

2011 ◽  
Vol 27 (5) ◽  
pp. 300-306 ◽  
Author(s):  
Yan-Jie Wang ◽  
Xing-Fu Li ◽  
Feng Ding ◽  
Qiang Shu ◽  
Li-Jun Song ◽  
...  
Keyword(s):  
Substance P ◽  
Dorsal Root Ganglion ◽  
Dorsal Root ◽  
Dorsal Root Ganglion Neurons ◽  
P Release ◽  
Ganglion Neurons ◽  
Substance P Release

Down regulation of TRPC1 by shRNA reduces mechanosensitivity in mouse dorsal root ganglion neurons in vitro

2009 ◽  
Vol 457 (1) ◽  
pp. 3-7 ◽  
Author(s):  
Susanne Staaf ◽  
Ingela Maxvall ◽  
Ulrika Lind ◽  
Johanna Husmark ◽  
Jan P. Mattsson ◽  
...  
Keyword(s):  
Dorsal Root Ganglion ◽  
Dorsal Root ◽  
Dorsal Root Ganglion Neurons ◽  
Down Regulation ◽  
Mouse Dorsal Root Ganglion ◽  
Ganglion Neurons

scholarly journals Insulin potentiates the response to mechanical stimuli in small dorsal root ganglion neurons and thin fibre muscle afferents in vitro

2019 ◽  
Vol 597 (20) ◽  
pp. 5049-5062 ◽  
Author(s):  
Norio Hotta ◽  
Kimiaki Katanosaka ◽  
Kazue Mizumura ◽  
Gary A. Iwamoto ◽  
Rie Ishizawa ◽  
...  
Keyword(s):  
Dorsal Root Ganglion ◽  
Dorsal Root ◽  
Muscle Afferents ◽  
Dorsal Root Ganglion Neurons ◽  
Mechanical Stimuli ◽  
Ganglion Neurons
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