scholarly journals Does exercise-induced hypoxemia modify lactate influx into erythrocytes and hemorheological parameters in athletes?

2004 ◽  
Vol 97 (3) ◽  
pp. 1053-1058 ◽  
Author(s):  
Philippe Connes ◽  
Didier Bouix ◽  
Guillaume Py ◽  
Corinne Caillaud ◽  
Pascale Kippelen ◽  
...  
Keyword(s):  
Blood Cells ◽  
Aerobic Power ◽  
Venous Blood ◽  
Maximal Aerobic Power ◽  
Monocarboxylate Transporter ◽  
Arterial Oxygen ◽  
Plasma Lactate ◽  
Monocarboxylate Transporter 1 ◽  
Arterial Oxygen Partial Pressure ◽  
Exercise Induced

This study investigated 1) red blood cells (RBC) rigidity and 2) lactate influxes into RBCs in endurance-trained athletes with and without exercise-induced hypoxemia (EIH). Nine EIH and six non-EIH subjects performed a submaximal steady-state exercise on a cyclo-ergometer at 60% of maximal aerobic power for 10 min, followed by 15 min at 85% of maximal aerobic power. At rest and at the end of exercise, arterialized blood was sampled for analysis of arterialized pressure in oxygen, and venous blood was drawn for analysis of plasma lactate concentrations and hemorheological parameters. Lactate influxes into RBCs were measured at three labeled [U-14C]lactate concentrations (1.6, 8.1, and 41 mM) on venous blood sampled at rest. The EIH subjects had higher maximal oxygen uptake than non-EIH ( P < 0.05). Total lactate influx was significantly higher in RBCs from EIH compared with non-EIH subjects at 8.1 mM (1,498.1 ± 87.8 vs. 1,035.9 ± 114.8 nmol·ml−1·min−1; P < 0.05) and 41 mM (2,562.0 ± 145.0 vs. 1,618.1 ± 149.4 nmol·ml−1·min−1; P < 0.01). Monocarboxylate transporter-1-mediated lactate influx was also higher in EIH at 8.1 mM ( P < 0.05) and 41 mM ( P < 0.01). The drop in arterial oxygen partial pressure was negatively correlated with total lactate influx measured at 8.1 mM ( r = −0.82, P < 0.05) and 41 mM ( r = −0.84, P < 0.05) in the two groups together. Plasma lactate concentrations and hemorheological data were similar in the two groups at rest and at the end of exercise. The results showed higher monocarboxylate transporter-1-mediated lactate influx in the EIH subjects and suggested that EIH could modify lactate influx into erythrocyte. However, higher lactate influx in EIH subjects was not accompanied by an increase in RBC rigidity.

Short-term training increases human muscle MCT1 and femoral venous lactate in relation to muscle lactate

1998 ◽  
Vol 274 (1) ◽  
pp. E102-E107 ◽  
Author(s):  
A. Bonen ◽  
K. J. A. McCullagh ◽  
C. T. Putman ◽  
E. Hultman ◽  
N. L. Jones ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Vastus Lateralis ◽  
Venous Blood ◽  
Maximal Oxygen Consumption ◽  
Bicycle Ergometer ◽  
Monocarboxylate Transporter ◽  
Short Term ◽  
Bicycle Ergometry ◽  
Muscle Lactate ◽  
Monocarboxylate Transporter 1

We examined the effects of increasing a known lactate transporter protein, monocarboxylate transporter 1 (MCT1), on lactate extrusion from human skeletal muscle during exercise. Before and after short-term bicycle ergometry training [2 h/day, 7 days at 65% maximal oxygen consumption (V˙o 2 max)], subjects ( n = 7) completed a continuous bicycle ergometer ride at 30%V˙o 2 max (15 min), 60%V˙o 2 max (15 min), and 75% V˙o 2 max (15 min). Muscle biopsy samples (vastus lateralis) and arterial and femoral venous blood samples were obtained before exercise and at the end of each workload. After 7 days of training the MCT1 content in muscle was increased (+18%; P < 0.05). The concentrations of both muscle lactate and femoral venous lactate were reduced during exercise ( P < 0.05) that was performed after training. High correlations were observed between muscle lactate and venous lactate before training ( r = 0.92, P < 0.05) and after training ( r = 0.85, P < 0.05), but the slopes of the regression lines between these variables differed markedly. Before training, the slope was 0.12 ± 0.01 mM lactate ⋅ mmol lactate−1 ⋅ kg muscle dry wt−1, and this was increased by 33% after training to 0.18 ± 0.02 mM lactate ⋅ mmol lactate−1 ⋅ kg muscle dry wt−1. This indicated that after training the femoral venous lactate concentrations were increased for a given amount of muscle lactate. These results suggest that lactate extrusion from exercising muscles is increased after training, and this may be associated with the increase in skeletal muscle MCT1.

Training does not protect against exhaustive exercise-induced lactate transport capacity alterations

2000 ◽  
Vol 278 (6) ◽  
pp. E1045-E1052 ◽  
Author(s):  
Nicolas Eydoux ◽  
Guillaume Py ◽  
Karen Lambert ◽  
Hervé Dubouchaud ◽  
Christian Préfaut ◽  
...  
Keyword(s):  
Endurance Training ◽  
Exhaustive Exercise ◽  
Monocarboxylate Transporter ◽  
Transport Capacity ◽  
Lactate Transport ◽  
Monocarboxylate Transporter 1 ◽  
Saturation Properties ◽  
Apparent Loss ◽  
Male Wistar Rats ◽  
Exercise Induced

The effects of endurance training on lactate transport capacity remain controversial. This study examined whether endurance training 1) alters lactate transport capacity, 2) can protect against exhaustive exercise-induced lactate transport alteration, and 3) can modify heart and oxidative muscle monocarboxylate transporter 1 (MCT1) content. Forty male Wistar rats were divided into control (C), trained (T), exhaustively exercised (E), and trained and exercised (TE) groups. Rats in the T and TE groups ran on a treadmill (1 h/day, 5 days/wk at 25 m/min, 10% incline) for 5 wk; C and E were familiarized with the exercise task for 5 min/day. Before being killed, E and TE rats underwent exhaustive exercise (25 m/min, 10% grade), which lasted 80 and 204 min, respectively ( P < 0.05). Although lactate transport measurements (zero- trans) did not differ between groups C and T, both E and TE groups presented an apparent loss of protein saturation properties. In the trained groups, MCT1 content increased in soleus (+28% for T and +26% for TE; P < 0.05) and heart muscle (+36% for T and +33% for TE; P < 0.05). Moreover, despite the metabolic adaptations typically observed after endurance training, we also noted increased lipid peroxidation byproducts after exhaustive exercise. We concluded that 1) endurance training does not alter lactate transport capacity, 2) exhaustive exercise-induced lactate transport alteration is not prevented by training despite increased MCT1 content, and 3) exercise-induced oxidative stress may enhance the passive diffusion responsible for the apparent loss of saturation properties, possibly masking lactate transport regulation.

Interindividual variation in total and carrier-mediated lactate influx into red blood cells

1998 ◽  
Vol 274 (4) ◽  
pp. R1025-R1030 ◽  
Author(s):  
Leena K. Väihkönen ◽  
A. Reeta Pösö
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Venous Blood ◽  
Age Groups ◽  
Lactate Concentration ◽  
Bimodal Distribution ◽  
Monocarboxylate Transporter ◽  
Interindividual Variation ◽  
Transport Activity ◽  
Major Carrier

To study in standardbred horses interindividual variation in the influx of lactate into red blood cells, venous blood samples were collected from 89 horses from 2 wk to 9 yr of age. For 62 horses, the rate of influx was normally distributed with a mean rate of 4.09 nmol ⋅ mg protein−1 ⋅ min−1at a lactate concentration of 10 mM, and the respective value for the other 27 horses was 0.58 nmol ⋅ mg protein−1 ⋅ min−1. At 30 mM of lactate, the rates were 8.71 and 1.97 nmol ⋅ mg protein−1 ⋅ min−1, respectively. This bimodal distribution was independent of age. In horses with high transport activity, the monocarboxylate transporter (MCT) appears to be the major carrier, whereas, in those with low transport activity, no activity of the MCT could be detected. The band 3 protein may account for 18–39% of transport activity. With all age groups combined, the transport activity tended to be higher in mares than in stallions. Lactate transport into red blood cells seems thus to be an inherent property in which participation of various transporters varies interindividually.

scholarly journals Differential Expression of Monocarboxylate Transporter 1 and Ancillary Protein CD147 in Red Blood Cells of Show Jumping Horses

2017 ◽  
Vol 50 ◽  
pp. 139-144
Author(s):  
Walter Heinz Feringer Júnior ◽  
Julia Ribeiro Garcia de Carvalho ◽  
Maria Luiza Mendes de Almeida ◽  
Eliana Gertrudes Macedo Lemos ◽  
Otávio Augusto Brioschi Soares ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Differential Expression ◽  
Blood Cells ◽  
Monocarboxylate Transporter ◽  
Monocarboxylate Transporter 1 ◽  
Show Jumping

scholarly journals Influence of gender, menstrual phase, and oral contraceptive use on immunological changes in response to prolonged cycling

2005 ◽  
Vol 99 (3) ◽  
pp. 979-985 ◽  
Author(s):  
Brian W. Timmons ◽  
Mazen J. Hamadeh ◽  
Michaela C. Devries ◽  
Mark A. Tarnopolsky
Keyword(s):  
Oral Contraceptive ◽  
Immune Cell ◽  
Aerobic Power ◽  
Statistical Significance ◽  
Venous Blood ◽  
Activity Level ◽  
Menstrual Phase ◽  
Cell Counts ◽  
Immunological Changes ◽  
Exercise Induced

This study determined the influence of gender, menstrual phase (MP), and oral contraceptive (OC) use on immunological changes in response to endurance exercise. Twelve women and 11 men similar in age, aerobic power, and activity level cycled for 90 min at 65% maximal aerobic power. Women were OC users ( n = 6) or nonusers (NOC) and cycled during the follicular (Fol) and the luteal (Lut) phases. Venous blood was collected before and after exercise to determine leukocyte counts, IL-6 concentrations, and cortisol. Higher resting levels of neutrophils (∼1.5-fold) and cortisol (∼2.5-fold) were found in OC vs. NOC and men. Exercise-induced immune cell count and IL-6 changes were similar between men and NOC, except for an ∼38% greater lymphocyte response in NOC vs. men ( P = 0.07). Neutrophil, monocyte, and lymphocyte responses to exercise during Lut in OC were greater than during Fol and also greater than the responses in men ( P ≤ 0.003). Changes in immune cell counts were consistently greater during Lut in OC vs. NOC, regardless of MP, but only neutrophil responses reached statistical significance ( P = 0.01). The exercise-induced change in IL-6 was ∼80% greater in NOC vs. OC during Fol ( P = 0.06), but it was similar between these groups during Lut. Cortisol changes with exercise were not different between groups or MP. These results highlight the necessity to control for gender, and in particular OC use, when designing studies evaluating exercise and immunology.

Ventilatory and cardiac responses to hypoxia at submaximal exercise are independent of altitude and exercise intensity

2012 ◽  
Vol 112 (4) ◽  
pp. 566-570 ◽  
Author(s):  
François J. Lhuissier ◽  
Maxime Brumm ◽  
Didier Ramier ◽  
Jean-Paul Richalet
Keyword(s):  
Exercise Intensity ◽  
Aerobic Power ◽  
Arterial Oxygen Saturation ◽  
Submaximal Exercise ◽  
Maximal Aerobic Power ◽  
Arterial Oxygen ◽  
Simulated Altitude ◽  
Cardiac Responses ◽  
The Individual ◽  
Hypoxic Exercise

The hypoxic exercise test combining a 4,800-m simulated altitude and a cycloergometer exercise at 30% of normoxic maximal aerobic power (MAP) is used to evaluate the individual chemosensitivity to hypoxia in submaximal exercise conditions. This test allows the calculation of three main parameters: the decrease in arterial oxygen saturation induced by hypoxia at exercise (ΔSae) and the ventilatory (HVRe) and cardiac (HCRe) responses to hypoxia at exercise. The aim of this study was to determine the influence of altitude and exercise intensity on the values of ΔSae, HVRe, and HCRe. Nine subjects performed hypoxic tests at three simulated altitudes (3,000 m, 4,000 m, and 4,800 m) and three exercise intensities (20%, 30%, and 40% MAP). ΔSae increased with altitude and was higher for 40% MAP than for 20% or 30% ( P < 0.05). For a constant heart rate, the loss in power output induced by hypoxia, relative to ΔSae, was independent of altitude (4,000–4,800 m) and of exercise intensity. HVRe and HCRe were independent of altitude (3,000–4,800 m) and exercise intensity (20%-40% MAP). Moreover, the intraindividual variability of responses to hypoxia was lower during moderate exercise than at rest ( P < 0.05 to P < 0.001). Therefore, we suggest that HVRe and HCRe are invariant parameters that can be considered as intrinsic physiological characteristics of chemosensitivity to hypoxia.

scholarly journals Differential Expression of Monocarboxylate Transporter 1 and Ancillary Protein CD147 in Red Blood Cells of Show Jumping Horses

2019 ◽  
Vol 81 ◽  
pp. 102791 ◽  
Author(s):  
Walter Heinz Feringer Júnior ◽  
Julia Ribeiro Garcia de Carvalho ◽  
Maria Luiza Mendes de Almeida ◽  
Eliana Gertrudes Macedo Lemos ◽  
Otávio Augusto Brioschi Soares ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Differential Expression ◽  
Blood Cells ◽  
Monocarboxylate Transporter ◽  
Monocarboxylate Transporter 1 ◽  
Show Jumping

scholarly journals Physical Exercise–Induced Hypoglycemia Caused by Failed Silencing of Monocarboxylate Transporter 1 in Pancreatic β Cells

2007 ◽  
Vol 81 (3) ◽  
pp. 467-474 ◽  
Author(s):  
Timo Otonkoski ◽  
Hong Jiao ◽  
Nina Kaminen-Ahola ◽  
Isabel Tapia-Paez ◽  
Mohammed S. Ullah ◽  
...  
Keyword(s):  
Physical Exercise ◽  
Monocarboxylate Transporter ◽  
Β Cells ◽  
Pancreatic Β Cells ◽  
Monocarboxylate Transporter 1 ◽  
Exercise Induced
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