Transcriptional profiling in mouse skeletal muscle following a single bout of voluntary running: evidence of increased cell proliferation

2005 ◽  
Vol 99 (6) ◽  
pp. 2406-2415 ◽  
Author(s):  
Sangdun Choi ◽  
Xuebin Liu ◽  
Ping Li ◽  
Takayuki Akimoto ◽  
Sun Young Lee ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Cell Proliferation ◽  
Transcriptional Profiling ◽  
Mouse Skeletal Muscle ◽  
Voluntary Running ◽  
Single Bout

Resident stem cells are not required for exercise-induced fiber-type switching and angiogenesis but are necessary for activity-dependent muscle growth

2006 ◽  
Vol 290 (6) ◽  
pp. C1461-C1468 ◽  
Author(s):  
Ping Li ◽  
Takayuki Akimoto ◽  
Mei Zhang ◽  
R. Sanders Williams ◽  
Zhen Yan
Keyword(s):  
Skeletal Muscle ◽  
Cell Proliferation ◽  
Stem Cell ◽  
Intraperitoneal Injection ◽  
Fiber Type ◽  
Muscle Growth ◽  
Plantaris Muscle ◽  
Voluntary Running ◽  
Exercise Induced ◽  
Activity Dependent

Skeletal muscle undergoes active remodeling in response to endurance exercise training, and the underlying mechanisms of this remodeling remain to be defined fully. We have recently obtained evidence that voluntary running induces cell cycle gene expression and cell proliferation in mouse plantaris muscles that undergo fast-to-slow fiber-type switching and angiogenesis after long-term exercise. To ascertain the functional role of cell proliferation in skeletal muscle adaptation, we performed in vivo 5-bromo-2′-deoxyuridine (BrdU) pulse labeling (a single intraperitoneal injection), which demonstrated a phasic increase (5- to 10-fold) in BrdU-positive cells in plantaris muscle between days 3 and 14 during 4 wk of voluntary running. Daily intraperitoneal injection of BrdU for 4 wk labeled 2.0% and 15.4% of the nuclei in plantaris muscle in sedentary and trained mice, respectively, and revealed the myogenic and angiogenic fates of the majority of proliferative cells. Ablation of resident stem cell activity by X-ray irradiation did not prevent voluntary running-induced increases of type IIa myofibers and CD31-positive endothelial cells but completely blocked the increase in muscle mass. These findings suggest that resident stem cell proliferation is not required for exercise-induced type IIb-to-IIa fiber-type switching and angiogenesis but is required for activity-dependent muscle growth. The origin of the angiogenic cells in this physiological exercise model remains to be determined.

scholarly journals Electroacupuncture suppresses myostatin gene expression: cell proliferative reaction in mouse skeletal muscle

2007 ◽  
Vol 30 (2) ◽  
pp. 102-110 ◽  
Author(s):  
Yutaka Takaoka ◽  
Mika Ohta ◽  
Akihiko Ito ◽  
Kunihiko Takamatsu ◽  
Aki Sugano ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Cell Proliferation ◽  
Satellite Cells ◽  
Expression Patterns ◽  
Low Frequency ◽  
Immunohistochemical Analysis ◽  
Long Term Effects ◽  
Transcription Control ◽  
Myostatin Gene ◽  
Mouse Skeletal Muscle

Complementary and alternative medicine (CAM) may provide patients with an alternative to traditional medicine, but an assessment of its efficacy is required. One CAM method, electroacupuncture (EA) treatment, is a maneuver that utilizes stimulation of acupuncture needles with a low-frequency microcurrent. To study the effect of short-term EA, we evaluated the differential expression of genes induced by EA in mouse skeletal muscle for up to 24 h. We then used RT-PCR to confirm the expression patterns of six differentially expressed genes. Bioinformatics analysis of their transcription control regions showed that EA-inducible genes have numerous common binding motifs that are related to cell differentiation, cell proliferation, muscle repair, and hyperplasia. These results suggested that EA treatment may induce cell proliferation in skeletal muscle. To verify this possibility, we used EA to stimulate mouse skeletal muscle daily for up to 1 mo and examined the long-term effects. Immunohistochemical analysis showed that nuclei of muscle cells treated with EA for 1 mo, especially nuclei of satellite cells, reacted with anti-human PCNA. Also, expression of the gene encoding myostatin, which is a growth repressor in muscle satellite cells, was suppressed by daily EA treatment for 1 wk; EA treatment for 1 mo resulted in more marked suppression of the gene. These molecular findings constitute strong evidence that EA treatment suppresses myostatin expression, which leads to a satellite cell-related proliferative reaction and repair in skeletal muscle.

scholarly journals The MicroRNA miR-696 is Regulated by SNARK and Reduces Mitochondrial Activity in Mouse Skeletal Muscle Through Pgc1α Inhibition

2021 ◽  
pp. 101226
Author(s):  
André L. Queiroz ◽  
Sarah J. Lessard ◽  
Amanda T. Ouchida ◽  
Hygor N. Araujo ◽  
Dawit A. Gonçalves ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Mitochondrial Activity ◽  
Mouse Skeletal Muscle
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