Expression and functional features of NaCT, a sodium-coupled citrate transporter, in human and rat livers and cell lines

2007 ◽  
Vol 292 (1) ◽  
pp. G402-G408 ◽  
Author(s):  
Elangovan Gopal ◽  
Seiji Miyauchi ◽  
Pamela M. Martin ◽  
Sudha Ananth ◽  
Sonne R. Srinivas ◽  
...  
Keyword(s):  
Cell Line ◽  
Cell Lines ◽  
Rat Liver ◽  
Liver Cell ◽  
Transport Activity ◽  
Primary Hepatocytes ◽  
Michaelis Constant ◽  
Functional Features ◽  
And Function ◽  
Rat Livers

In this article, we report on the expression and function of a Na+-coupled transporter for citrate, NaCT, in human and rat liver cell lines and in primary hepatocytes from the rat liver. We also describe the polarized expression of this transporter in human and rat livers. Citrate uptake in human liver cell lines HepG2 and Huh-7 was obligatorily dependent on Na+. The uptake system showed a preference for citrate over other intermediates of the citric acid cycle and exhibited a Michaelis constant of ∼6 mM for citrate. The transport activity was stimulated by Li+, and the activation was associated with a marked increase in substrate affinity. Citrate uptake in rat liver cell line MH1C1 was also Na+ dependent and showed a preference for citrate. The Michaelis constant for citrate was ∼10 μM. The transport activity was inhibited by Li+. Primary hepatocytes from the rat liver also showed robust activity for Na+-coupled citrate uptake, with functional features similar to those described in the rat liver cell line. Immunolabeling with a specific anti-NaCT antibody showed exclusive expression of the transporter in the sinusoidal membrane of hepatocytes in human and rat livers. This constitutes the first report on the expression and function of NaCT in liver cells.

The nuclear 3,5,3'-triiodothyronine receptor in human leukaemic cell lines

1984 ◽  
Vol 105 (3) ◽  
pp. 429-432 ◽  
Author(s):  
Juan Bernal ◽  
Leif C. Andersson
Keyword(s):  
Growth Hormone ◽  
Cell Line ◽  
Cell Lines ◽  
Rat Liver ◽  
Association Constant ◽  
Erythroid Differentiation ◽  
Leukaemic Cell ◽  
Cells In Culture ◽  
Gh Cells ◽  
High Concentrations

Abstract. The 3,5,3'-triiodothyronine (T3) receptor has been studied in a series of continuously growing human leukaemic cell lines. High concentrations of receptor were found in the erythroblastoid cell line K-562. T3 was bound to the nuclei of these cells with an association constant of 3.4 × 109 m−1, and capacity 104 fmol/100 μg DNA, or 8700 molecules/nucleus. This capacity is comparable to that of rat liver or growth hormone producing cells (GH cells) in culture, and suggests that the K-562 cell line could be a useful model for the study of T3 action on erythroid differentiation.

Survival and Function of Fibroblasts Stored as Stock Cultures at a Non-freezing Temperature

1993 ◽  
Vol 21 (2) ◽  
pp. 206-209
Author(s):  
Anders H. G. Andrén ◽  
Anders P. Wieslander
Keyword(s):  
Cell Growth ◽  
Cell Line ◽  
Cell Lines ◽  
Fibroblast Cell ◽  
Freezing Temperature ◽  
Mouse Fibroblast ◽  
Toxic Response ◽  
Normal Manner ◽  
And Function

Cytotoxicity, measured as inhibition of cell growth of cultured cell lines, is a widely used method for testing the safety of biomaterials and chemicals. One major technical disadvantage with this method is the continuous routine maintenance of the cell lines. We decided to investigate the possibility of storing stock cultures of fibroblasts (L-929) in an ordinary refrigerator as a means of reducing the routine workload. Stock cultures of the mouse fibroblast cell line L-929 were prepared in plastic vials with Eagle's minimum essential medium. The vials were stored in a refrigerator at 4–10°C for periods of 7–31 days. The condition of the cells after storage was determined as cell viability, cell growth and the toxic response to acrylamide, measured as cell growth inhibition. We found that the L-929 cell line can be stored for 2–3, weeks with a viabilty > 90% and a cell growth of about 95%, compared to L-929 cells grown and subcultured in the normal manner. The results also show that the toxic response to acrylamide, using refrigerator stored L-929 cells, corresponds to that of control L-929 cells. We concluded that it is possible to store L-929 cells in a refrigerator for periods of up to 3 weeks and still use the cells for in vitro cytotoxic assays.

Glucose metabolism by adult hepatocytes in primary culture and by cell lines from rat liver

1978 ◽  
Vol 234 (3) ◽  
pp. C122-C130 ◽  
Author(s):  
D. M. Bissell ◽  
G. A. Levine ◽  
M. J. Bissell
Keyword(s):  
Glucose Metabolism ◽  
Primary Culture ◽  
Cell Lines ◽  
Rat Liver ◽  
Liver Cell ◽  
Time Course ◽  
Primary Cultures ◽  
Functional Change ◽  
Permanent Cell ◽  
Rat Hepatoma

The metabolic fate of [U-14C]glucose has been examined in detail in adult rat hepatocytes in primary monolayer culture, as well as in two permanent cell lines--Buffalo rat liver (BRL) and transplantable rat hepatoma (HTC) cells-derived from normal rat liver and from rat hepatoma, respectively. Under defined conditions of incubation, at a glucose concentration of 5.5 mM, the three types of cultured liver cells exhibited pronounced differences in glucose metabolism. Primary cultures, like the intact liver, differed from the cell lines in consuming relatively small amounts of glucose and converting approximately 50% of the total metabolized glucose to lactate. By contrast, the permantent cell lines consumed glucose at a 40-fold greater rate than did primary cultures, converting 80--90% of the carbohydrate to lactate. Oxidative metabolism of glucose carbon also differed among the three types of liver culture. Of the total [U-14C]glucose consumed, primary cultures converted approximately 30% to labeled CO2 per hour, whereas the liver cell lines converted 5--10%. Finally, glucose metabolism in primary culture exhibited adaptation as hepatocytes aged in culture, shifting progressively toward the pattern exhibited by the permanent cell lines. This change occurred over a time course similar to that for other kinds of functional change in hepatocytes in primary culture and thus may be relevant to the general problem of phenotypic alteration in liver cell culture.

scholarly journals Pancreatic Transdifferentiation and Glucose-Regulated Production of Human Insulin in the H4IIE Rat Liver Cell Line

2016 ◽  
Vol 17 (4) ◽  
pp. 534 ◽  
Author(s):  
Binhai Ren ◽  
Chang Tao ◽  
Margaret Swan ◽  
Nichole Joachim ◽  
Rosetta Martiniello-Wilks ◽  
...  
Keyword(s):  
Cell Line ◽  
Human Insulin ◽  
Rat Liver ◽  
Liver Cell ◽  
Liver Cell Line

Multiplication stimulating activity (MSA) from the BRL 3A rat liver cell line: Relation to human somatomedins and insulin

1981 ◽  
Vol 15 (3) ◽  
pp. 253-286 ◽  
Author(s):  
Matthew M. Rechler ◽  
S. Peter Nissley ◽  
George L. King ◽  
Alan C. Moses ◽  
Ellen E. Van Obberghen-Schilling ◽  
...  
Keyword(s):  
Cell Line ◽  
Rat Liver ◽  
Liver Cell ◽  
Liver Cell Line

A characterization of the ZFL cell line and primary hepatocytes as in vitro liver cell models for the zebrafish (Danio rerio)

2014 ◽  
Vol 147 ◽  
pp. 7-17 ◽  
Author(s):  
Marta Eide ◽  
Marte Rusten ◽  
Rune Male ◽  
Knut Helge Midtbø Jensen ◽  
Anders Goksøyr
Keyword(s):  
Cell Line ◽  
Danio Rerio ◽  
Liver Cell ◽  
Cell Models ◽  
Primary Hepatocytes

Thyroid hormone induction of Na(+)-K(+)-ATPase and its mRNAs in a rat liver cell line

1990 ◽  
Vol 258 (3) ◽  
pp. C544-C551 ◽  
Author(s):  
G. G. Gick ◽  
F. Ismail-Beigi
Keyword(s):  
Atpase Activity ◽  
Cell Line ◽  
Rat Liver ◽  
Liver Cell ◽  
Northern Blot Analysis ◽  
Fold Increase ◽  
Beta Subunits ◽  
Inhibit Protein Synthesis ◽  
Liver Cell Line ◽  
Stimulation Of

The expression of mRNAs encoding the alpha- and beta-subunits of Na(+)-K(+)-ATPase (Na(+)-K+ pump) was examined in a rat liver cell line, Clone 9, in various thyroidal states. Northern blot analysis of total RNA isolated from cells incubated in hypothyroid serum-containing medium revealed the expression of mRNAs encoding Na(+)-K(+)-ATPase alpha 1-(mRNA alpha 1) and beta- (mRNA beta) subunits; mRNAs encoding the alpha 2- and alpha 3-subunits were undetectable. There was a discrepancy in the abundance of mRNA alpha 1 relative to mRNA beta such that mRNA alpha 1 exceeded the sum of the multiple mRNA beta bands by approximately 35-fold. 3,3',5-Triiodothyronine (T3) produced a coordinate augmentation of mRNA alpha 1 and mRNA beta contents that was demonstrable within 2 h and preceded the stimulation of Na(+)-K(+)-ATPase activity. After incubation of cells with T3 for 48 h, Na(+)-K(+)-ATPase activity was stimulated by 1.32-fold, whereas mRNA alpha 1 and mRNA beta abundances were increased 1.46- and 2.87-fold, respectively. Treatment of cells for 6 h with 10 micrograms/ml cycloheximide, a concentration sufficient to inhibit protein synthesis by 95%, elicited a 3.5- and 5.1-fold increase in mRNA alpha 1 and mRNA beta content, respectively. Cycloheximide abrogated the stimulatory effect of T3 on mRNA beta abundance, whereas the T3-induced increase in mRNA alpha 1 content was not prevented.(ABSTRACT TRUNCATED AT 250 WORDS)

Stimulation of Active Na+and K+Transport by Thyroid Hormone in a Rat Liver Cell Line: Role of Enhanced Na+Entry*

Endocrinology ◽  
1986 ◽  
Vol 119 (6) ◽  
pp. 2527-2536 ◽  
Author(s):  
FARAMARZ ISMAIL-BEIGI ◽  
RICHARD S. HABER ◽  
JOHN N. LOEB
Keyword(s):  
Thyroid Hormone ◽  
Cell Line ◽  
Rat Liver ◽  
Liver Cell ◽  
Liver Cell Line ◽  
K Transport ◽  
Stimulation Of

Inhibitory Effects of Capsaicinoids on Fatty Acid Desaturation in a Rat Liver Cell Line

2001 ◽  
Vol 65 (8) ◽  
pp. 1859-1863 ◽  
Author(s):  
Nobuhiro NAKANO ◽  
Norifumi SHIRASAKA ◽  
Kazuki MASUOKA ◽  
Tetsuo MURAKAMI ◽  
Tatsuo WATANABE ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Cell Line ◽  
Rat Liver ◽  
Liver Cell ◽  
Fatty Acid Desaturation ◽  
Inhibitory Effects ◽  
Liver Cell Line
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