Satellite cells and utrophin are not directly correlated with the degree of skeletal muscle damage in mdx mice

2005 ◽  
Vol 289 (1) ◽  
pp. C42-C48 ◽  
Author(s):  
Akira Yamane ◽  
Satonari Akutsu ◽  
Thomas G. H. Diekwisch ◽  
Ryoichi Matsuda
Keyword(s):  
Skeletal Muscle ◽  
Muscle Damage ◽  
Satellite Cells ◽  
Skeletal Muscles ◽  
Expression Level ◽  
Mdx Mice ◽  
Cell Nuclei ◽  
Muscle Satellite Cells ◽  
Skeletal Muscle Damage ◽  
Muscle Tissues

To determine whether muscle satellite cells and utrophin are correlated with the degree of damage in mdx skeletal muscles, we measured the area of the degenerative region as an indicator of myofiber degeneration in the masseter, gastrocnemius, soleus, and diaphragm muscles of mdx mice. Furthermore, we analyzed the expression levels of the paired box homeotic gene 7 ( pax7), m-cadherin (the makers of muscle satellite cells), and utrophin mRNA. We also investigated the immunolocalization of m-cadherin and utrophin proteins in the muscles of normal C57BL/10J (B10) and mdx mice. The expression level of pax7 mRNA and the percentage of m-cadherin-positive cells among the total number of cell nuclei in the muscle tissues in all four muscles studied were greater in the mdx mice than in the B10 mice. However, there was no significant correlation between muscle damage and expression level for pax7 mRNA ( R = −0.140), nor was there a correlation between muscle damage and the percentage of satellite cells among the total number of cell nuclei ( R = −0.411) in the mdx mice. The expression level of utrophin mRNA and the intensity of immunostaining for utrophin in all four muscles studied were greater in the mdx mice than in the B10 mice. However, there also was not a significant correlation between muscle damage and expression level of utrophin mRNA ( R = 0.231) in the mdx mice, although upregulated utrophin was incorporated into the sarcolemma. These results suggest that satellite cells and utrophin are not directly correlated with the degree of skeletal muscle damage in mdx mice.

scholarly journals Ubiquitin-specific peptidase 8 regulates proliferation and early differentiation of sheep skeletal muscle satellite cells

2021 ◽  
Vol 66 (No. 3) ◽  
pp. 87-96
Author(s):  
Shaoyu Wang ◽  
Kui Li ◽  
Hui Gao ◽  
Zepeng Liu ◽  
Shuang Shi ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Satellite Cells ◽  
Mrna Level ◽  
Expression Level ◽  
Muscle Satellite Cells ◽  
Early Differentiation ◽  
Skeletal Muscle Satellite Cells ◽  
Proliferation And Differentiation ◽  
Specific Protease ◽  
Ubiquitin Specific Protease

Ubiquitin-specific protease 8 (USP8), a member of the ubiquitin-specific protease (USP) family, was originally identified as playing a role in the regulation of growth and cell cycle. However, its functional role in myogenesis is unknown. In this study, we investigated the role of USP8 in proliferation and differentiation of sheep skeletal muscle satellite cells. The results showed that the expression level of USP8 was significantly increased on days 2 and 3 following the induction of the differentiation process. Furthermore, knocking down USP8 resulted in a significant increase in myogenin-positive cells, and promoted early differentiation of satellite cells by regulating the expression level of paired box 7 (PAX7). Additionally, knocking down USP8 suppressed muscle satellite cell proliferation, possibly explaining that the relative mRNA level of USP8 was linearly related to muscle fibre density of Hu sheep. Overall, our research demonstrates that USP8 plays a role in proliferation and early differentiation of skeletal muscle satellite cells.

scholarly journals miR-21-5p Regulates the Proliferation and Differentiation of Skeletal Muscle Satellite Cells by Targeting KLF3 in Chicken

Genes ◽  
2021 ◽  
Vol 12 (6) ◽  
pp. 814
Author(s):  
Donghao Zhang ◽  
Jinshan Ran ◽  
Jingjing Li ◽  
Chunlin Yu ◽  
Zhifu Cui ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Satellite Cells ◽  
Muscle Development ◽  
Target Genes ◽  
Cell Number ◽  
Sequencing Data ◽  
Proliferation Markers ◽  
Muscle Satellite Cells ◽  
Skeletal Muscle Satellite Cells ◽  
Proliferation And Differentiation

The proliferation and differentiation of skeletal muscle satellite cells (SMSCs) play an important role in the development of skeletal muscle. Our previous sequencing data showed that miR-21-5p is one of the most abundant miRNAs in chicken skeletal muscle. Therefore, in this study, the spatiotemporal expression of miR-21-5p and its effects on skeletal muscle development of chickens were explored using in vitro cultured SMSCs as a model. The results in this study showed that miR-21-5p was highly expressed in the skeletal muscle of chickens. The overexpression of miR-21-5p promoted the proliferation of SMSCs as evidenced by increased cell viability, increased cell number in the proliferative phase, and increased mRNA and protein expression of proliferation markers including PCNA, CDK2, and CCND1. Moreover, it was revealed that miR-21-5p promotes the formation of myotubes by modulating the expression of myogenic markers including MyoG, MyoD, and MyHC, whereas knockdown of miR-21-5p showed the opposite result. Gene prediction and dual fluorescence analysis confirmed that KLF3 was one of the direct target genes of miR-21-5p. We confirmed that, contrary to the function of miR-21-5p, KLF3 plays a negative role in the proliferation and differentiation of SMSCs. Si-KLF3 promotes cell number and proliferation activity, as well as the cell differentiation processes. Our results demonstrated that miR-21-5p promotes the proliferation and differentiation of SMSCs by targeting KLF3. Collectively, the results obtained in this study laid a foundation for exploring the mechanism through which miR-21-5p regulates SMSCs.

Cardiorespiratory and skeletal muscle damage due to COVID-19: making the urgent case for rehabilitation

2021 ◽  
pp. 1-14
Author(s):  
Rebeca Nunes Silva ◽  
Cássia Da Luz Goulart ◽  
Murilo Rezende Oliveira ◽  
Guilherme Yassuyuki Tacao ◽  
Guilherme Dionir Back ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Muscle Damage ◽  
Skeletal Muscle Damage ◽  
Urgent Case

Modifications in the Concentrations of Circulating Myoglobin after Treatment with Low Doses of Adriamycin

1985 ◽  
Vol 71 (5) ◽  
pp. 463-468 ◽  
Author(s):  
Giovanni Carulli ◽  
Aldo Clerico ◽  
Alessandra Marini ◽  
Maria Grazia Del Chicca ◽  
Renato Vanacore ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Creatine Kinase ◽  
Cancer Patients ◽  
Muscle Damage ◽  
Low Doses ◽  
Skeletal Muscle Damage ◽  
Creatine Kinase Mb

The modifications in the concentration of circulating myoglobin have been studied by means of a radioimmunoassay in 15 cancer patients undergoing polychemotherapy including adriamycin. In 8 patients significant increases in myoglobin levels were found after injection of low doses of the drug (25-50 mg/m2). Moreover, a disturbance of the normal biorhythm of the protein was evident in 12 patients. Creatine kinase-MB was evaluated by means of a radioimmunoassay, but there was no relation between an increase in the isoenzyme and an increase in myoglobin. No ECG modifications were detected. These data indicate that the measurement of myoglobin may offer an indication of myocardial or skeletal muscle damage caused by adriamycin.

Isoform specific ALT measurement during liver and skeletal muscle damage

2012 ◽  
Vol 211 ◽  
pp. S48
Author(s):  
Björn Glinghammar ◽  
Ingalill Rafter ◽  
Ina Schuppe-Koistinen ◽  
Ian Cotgreave
Keyword(s):  
Skeletal Muscle ◽  
Muscle Damage ◽  
Skeletal Muscle Damage
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