Alteration in expression of myosin isoforms in detrusor smooth muscle following bladder outlet obstruction

2003 ◽  
Vol 285 (6) ◽  
pp. C1397-C1410 ◽  
Author(s):  
Michael E. DiSanto ◽  
Raimund Stein ◽  
Shaohua Chang ◽  
Joseph A. Hypolite ◽  
Yongmu Zheng ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Bladder Outlet Obstruction ◽  
Rabbit Model ◽  
Outlet Obstruction ◽  
Maximum Velocity ◽  
Bladder Function ◽  
Myosin Isoforms ◽  
Detrusor Smooth Muscle ◽  
Myosin Heavy Chain Isoform ◽  
Essential Light Chain

Partial urinary bladder outlet obstruction (PBOO) in men, secondary to benign prostatic hyperplasia, induces detrusor smooth muscle (DSM) hypertrophy. However, despite DSM hypertrophy, some bladders become severely dysfunctional (decompensated). Using a rabbit model of PBOO, we found that although DSM from sham-operated bladders expressed nearly 100% of both the smooth muscle myosin heavy chain isoform SM-B and essential light chain isoform LC17a, DSM from severely dysfunctional bladders expressed as much as 75% SM-A and 40% LC17b(both associated with decreased maximum velocity of shortening). DSM from dysfunctional bladder also exhibited tonic-type contractions, characterized by slow force generation and high force maintenance. Immunofluorescence microscopy showed that decreased SM-B expression in dysfunctional bladders was not due to generation of a new cell population lacking SM-B. Metabolic cage monitoring revealed decreased void volume and increased voiding frequency correlated with overexpression of SM-A and LC17b. Myosin isoform expression and bladder function returned toward normal upon removal of the obstruction, indicating that the levels of expression of these isoforms are markers of the PBOO-induced dysfunctional bladders.

scholarly journals Protective Effects of Antimuscarinics on the Bladder Remodeling After Bladder Outlet Obstruction

2017 ◽  
Vol 44 (3) ◽  
pp. 907-919 ◽  
Author(s):  
Qiang Liu ◽  
Deyi Luo ◽  
Tongxin Yang ◽  
Banghua Liao ◽  
Hong Li ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Bladder Outlet Obstruction ◽  
Outlet Obstruction ◽  
Structure And Function ◽  
Bladder Function ◽  
Bladder Smooth Muscle ◽  
M3 Receptors ◽  
And Function

Background/Aims: Overactive bladder associated with bladder outlet obstruction (BOO) is a highly prevalent condition, which is usually treated with antimuscarinics. However, the potential effects of antimuscarinics on the structure and function of bladder have not been investigated thus far. Methods: Sprague-Dawley(R) rats accepted bladder neck obstruction surgery or sham surgery, and then received treatment of three different antimuscarinics (Solifenacin, Darifenacin, and Tolterodine) or vehicle. After 3, 6 and 12 weeks, the bladder function and structure were measured. The effect of antimuscarinics on cellular alteration in vitro was observed under mechanical stimulation. Bladder morphology were examined by immunohistochemistry, and the bladder function were investigated by cystometry and strip contractility test. The expression of muscarinic receptors and inflammatory cytokines were measured by PCR and Western blotting. Results: Here we demonstrate, both in vitro and in vivo, that antimuscarinics are protective regulators for the bladder structure and function. Antimuscarinics decrease the weight of bladders with BOO. Antimuscarinics improve the voiding parameter and enhance the contraction of bladder smooth muscle. The results also show that antimuscarinics inhibit the proliferation of bladder smooth muscle cells both in vivo and in vitro, it can reduce the collagen deposition and inflammatory cytokines in bladders with BOO. During this process, the expression of M2 and M3 receptors was altered by antimuscarinics. Conclusion: Antimuscarinics could reverse the structural and functional changes of BOO bladder wall at cellular and tissue level, and the alteration of M2 and M3 receptors may be involved in this biological process.

REGIONAL ALTERATIONS IN THE EXPRESSION OF SMOOTH MUSCLE MYOSIN ISOFORMS IN RESPONSE TO PARTIAL BLADDER OUTLET OBSTRUCTION

2005 ◽  
Vol 173 (1) ◽  
pp. 302-308 ◽  
Author(s):  
ANITA S. MANNIKAROTTU ◽  
JOSEPH A. HYPOLITE ◽  
STEPHEN A. ZDERIC ◽  
ALAN J. WEIN ◽  
SAMUEL CHACKO ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Bladder Outlet Obstruction ◽  
Outlet Obstruction ◽  
Smooth Muscle Myosin ◽  
Myosin Isoforms ◽  
Partial Bladder Outlet Obstruction ◽  
Muscle Myosin

scholarly journals Urinary Bladder Contraction and Relaxation: Physiology and Pathophysiology

2004 ◽  
Vol 84 (3) ◽  
pp. 935-986 ◽  
Author(s):  
Karl-Erik Andersson ◽  
Anders Arner
Keyword(s):  
Smooth Muscle ◽  
Urinary Bladder ◽  
Bladder Wall ◽  
Outlet Obstruction ◽  
Hormonal Control ◽  
Membrane Properties ◽  
Bladder Function ◽  
Future Research ◽  
Detrusor Smooth Muscle ◽  
Functional Changes

The detrusor smooth muscle is the main muscle component of the urinary bladder wall. Its ability to contract over a large length interval and to relax determines the bladder function during filling and micturition. These processes are regulated by several external nervous and hormonal control systems, and the detrusor contains multiple receptors and signaling pathways. Functional changes of the detrusor can be found in several clinically important conditions, e.g., lower urinary tract symptoms (LUTS) and bladder outlet obstruction. The aim of this review is to summarize and synthesize basic information and recent advances in the understanding of the properties of the detrusor smooth muscle, its contractile system, cellular signaling, membrane properties, and cellular receptors. Alterations in these systems in pathological conditions of the bladder wall are described, and some areas for future research are suggested.

Partial Bladder Outlet Obstruction Selectively Abolishes Protein Kinase C Induced Contraction of Rabbit Detrusor Smooth Muscle

2006 ◽  
Vol 176 (6) ◽  
pp. 2716-2721 ◽  
Author(s):  
Michaela C. Stanton ◽  
J. Christopher Austin ◽  
Daniel P. Delaney ◽  
Alex Gosfield ◽  
James O. Marx ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Protein Kinase C ◽  
Protein Kinase ◽  
Bladder Outlet Obstruction ◽  
Outlet Obstruction ◽  
Detrusor Smooth Muscle ◽  
Partial Bladder Outlet Obstruction ◽  
Kinase C
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