Subcellular localization of Apaf-1 in apoptotic rat pituitary cells

2006 ◽  
Vol 290 (3) ◽  
pp. C672-C677 ◽  
Author(s):  
Maja Potokar ◽  
Marko Kreft ◽  
Helena H. Chowdhury ◽  
Nina Vardjan ◽  
Robert Zorec
Keyword(s):  
Cytochrome C ◽  
Single Cells ◽  
Cellular Level ◽  
Pituitary Cells ◽  
Apoptotic Pathway ◽  
Proliferation And Apoptosis ◽  
Rat Pituitary ◽  
Before And After ◽  
Rat Pituitary Cells ◽  
First Time

A key step in the intrinsic apoptotic pathway is the assembly of the apoptosome complex. The apoptosome components are well known; however, the physiology of the assembly of the apoptosome complex at the cellular level is still poorly defined. The aim of this work was to study the subcellular distribution of the apoptosome scaffold protein apoptotic protease-activating factor 1 (Apaf-1) before and after triggering apoptosis in single somatotrophs. Somatotrophs are the subject of extensive pituitary tissue remodeling in different physiological situations in which the quality and the number of pituitary cells are determined by cell proliferation and apoptosis. We show herein that 2 h after triggering apoptosis with rotenone, Apaf-1 redistributed to the proximity of mitochondria. In addition, the degree of colocalization between Apaf-1 and fluorescently labeled caspase-9 significantly increased during the same period. Furthermore, we show herein for the first time in single cells that the colocalization between Apaf-1 and cytochrome c increases only transiently, indicating a transient interaction between cytochrome c and Apaf-1 during the activation of apoptosis in these cells.

High concentrations of immunoreactive inhibin in the plasma of mares and fetal gonads during the second half of pregnancy

1996 ◽  
Vol 8 (8) ◽  
pp. 1137 ◽  
Author(s):  
Y Nambo ◽  
S Nagata ◽  
M Oikawa ◽  
T Yoshihara ◽  
N Tsunoda ◽  
...  
Keyword(s):  
Immunohistochemical Staining ◽  
Follicle Stimulating Hormone ◽  
Plasma Concentrations ◽  
Peripheral Circulation ◽  
Pituitary Cells ◽  
Rat Pituitary ◽  
High Concentrations ◽  
Rat Pituitary Cells ◽  
First Time

Plasma concentrations of immunoreactive (ir)-inhibin were measured in seven pregnant mares from around Day 140 of gestation to Day 2 after parturition using a heterologous bovine-based radioimmunoassay (RIA). Concentrations of luteinizing hormone (LH), follicle-stimulating hormone (FSH), oestradiol-17 beta, progesterone and relaxin were also measured in the same samples. A marked increase in plasma concentrations of ir-inhibin, FSH and LH occurred between Day 220 and Day 300 of gestation but the concentrations of all three hormones returned to baseline by about Day 320 (three weeks before parturition). In contrast, circulating concentrations of the three placental hormones, oestradiol-17 beta, progesterone and relaxin, increased during the final weeks of pregnancy and then decreased markedly to basal values within two days of parturition. There was a positive correlation between circulating concentrations of ir-inhibin and FSH (r = 0.75, P < 0.01) rather than the expected negative correlation. ir-inhibin was not detected in homogenates obtained at Day 190 of pregnancy and form term placenta, but high concentrations of ir-inhibin were present in homogenates of fetal and newborn gonads. Despite the high concentrations of ir-inhibin in these homogenates, they failed to exert any suppressive bioactivity on FSH secretion by rat pituitary cells cultured in vitro. Furthermore, immunohistochemical staining revealed the presence of inhibin in the interstitial cells of equine fetal gonads at Day 190 of gestation. These findings demonstrate for the first time that high concentrations of ir-inhibin, LH and FSH are secreted into the peripheral circulation of the mare during the second half of pregnancy. However, ir-inhibin present in the plasma of pregnant mares appears to be biologically inactive. This hormone is not presumed to be of placental origin but it is proposed that either the enlarged fetal gonads or the maternal ovaries, or both of these organs, may be a source of inhibin in response to the coincident increase in circulating concentrations of LH and FSH.

SPONTANEOUS RELEASE OF IMMUNOREACTIVE ACTH FROM ISOLATED RAT PITUITARY CELLS

1974 ◽  
Vol 77 (1_Suppl) ◽  
pp. S162
Author(s):  
H. L. Fehm ◽  
K. H. Voigt ◽  
R. Lang ◽  
M. Schleyer ◽  
E. F. Pfeiffer
Keyword(s):  
Pituitary Cells ◽  
Spontaneous Release ◽  
Rat Pituitary ◽  
Rat Pituitary Cells ◽  
Isolated Rat

Modulation of prolactin secretion by contraceptive progestins in cultured rat pituitary cells in vitro

1988 ◽  
Vol 117 (4_Suppl) ◽  
pp. S188-S189
Author(s):  
L. KIESEL ◽  
T. RABE ◽  
D. SCHOLZ ◽  
V. KIRSCHNER ◽  
B. RUNNEBAUM
Keyword(s):  
Prolactin Secretion ◽  
Pituitary Cells ◽  
Rat Pituitary ◽  
Rat Pituitary Cells

Effects of chlorobenzenes on the histamine (HA) activated arginine-vasopressin (AVP) release -- from rat pituitary cells

2013 ◽  
Author(s):  
Zsuzsanna Valkusz ◽  
Zsolt Molnar ◽  
Peter Hausinger ◽  
Mariann Radacs ◽  
Marta Galfi ◽  
...  
Keyword(s):  
Arginine Vasopressin ◽  
Pituitary Cells ◽  
Rat Pituitary ◽  
Rat Pituitary Cells

scholarly journals CircAgtpbp1 Acts as a Molecular Sponge of miR-543-5p to Regulate the Secretion of GH in Rat Pituitary Cells

Animals ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 558
Author(s):  
ZeWen Yu ◽  
WenZhi Ren ◽  
Tian Wang ◽  
WeiDi Zhang ◽  
ChangJiang Wang ◽  
...  
Keyword(s):  
Pituitary Gland ◽  
Inhibitory Effect ◽  
Pituitary Cells ◽  
Sequencing Analysis ◽  
Hormone Regulation ◽  
Gh Secretion ◽  
Qrt Pcr ◽  
Rat Pituitary ◽  
Rat Pituitary Cells

CircRNAs have been identified to be expressed differently and stably in numerous species and tissues, but their functions in growth hormone (GH) secretion are still largely unknown. In summary, we have revealed a circRNA-miRNA-mRNA network that may play a biological role in the rat pituitary gland. First, we verified the chromosome location information of circAgtpbp1 according to sequencing analysis. The circAgtpbp1 characteristics were authenticated through PCR, qRT–PCR, treating with RNase and fluorescent in situ hybridization (FISH). Second, we detected the expression pattern of circAgtpbp1 in the rat anterior pituitary by qRT–PCR. We also designed circAgtpbp1 siRNA and constructed overexpression plasmid to evaluate the effect of circAgtpbp1 function on GH secretion by qRT–PCR, ELISA and Western blot. CircAgtpbp1 is a stable, truly circular molecule. We found that circAgtpbp1 interacted with miR-543-5p and can regulate GH secretion in pituitary cells through a circAgtpbp1-miR-543-5p-GH axis. Overall, the evidence generated by our study suggests that circAgtpbp1 can act as a sponge of miR-543-5p to reduce the inhibitory effect of miR-543-5p on Gh1 and further promote GH secretion. These findings expand our existing knowledge on the mechanisms of hormone regulation in the pituitary gland.

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