Are chicken embryos endotherms or ectotherms? A laboratory exercise integrating concepts in thermoregulation and metabolism

2007 ◽  
Vol 31 (1) ◽  
pp. 97-109 ◽  
Author(s):  
Sara M. Hiebert ◽  
Jocelyne Noveral

This investigative laboratory exercise uses the different relations between ambient temperature and metabolic rate in endotherms and ectotherms as a core concept to answer the following question: What thermoregulatory mode is employed by chicken embryos? Emphasis is placed on the physiological concepts that can be taught with this exercise, including methods for measuring rates of oxygen consumption, the relation between oxygen consumption and metabolic rate, the influence of temperature on metabolic rate, and the differences between endotherms and ectotherms both in the overall magnitude of metabolic rate and in the shape of the relation between metabolic rate and ambient temperature. Included in this article are respirometer designs suitable for teachers working with a wide variety of budgets and available equipment, specific laboratory protocols for collecting data, sample data, thought questions with sample answers, and suggestions for classroom implementation as a 1-, 2- or 3-wk laboratory exercise that can be taught at a variety of undergraduate levels.

1987 ◽  
Vol 119 (10) ◽  
pp. 941-954 ◽  
Author(s):  
Kees van Frankenhuyzen ◽  
Carl W. Nystrom

AbstractSpruce budworm larvae were bioassayed against Bacillus thuringiensis Berliner to study the effect of temperature on the expression of toxicity. Temperatures between 16 and 28°C did not affect the ultimate level of toxicity (LC50). However, LT50’s increased from 2–8 days at 28°C to 11–20 days at 16°C, depending on concentration of the pathogen. When larvae were force-fed with a single dose, temperature had a similar effect on the time course of mortality without affecting the level of mortality. Feeding inhibition of force-fed larvae commenced immediately after dosing. Larvae that did not recover died without further feeding, even at lower temperatures when death occurred 2–3 weeks after dosing. Recovering larvae resumed feeding after 2 (28°C) to 6 (13°C) days. Recovered larvae took longer to develop and produced lighter pupae than untreated larvae. Our data suggest that temperature-dependent feeding and recovery did not contribute to quicker death at higher temperatures. Expression of the toxin itself appears to depend on temperature, possibly through the influence of temperature on metabolic rate of affected gut cells. Implications of these findings for the efficacy of spruce budworm control operations are discussed.


Author(s):  
Willie J. Van Aardt ◽  
Japie Mienie ◽  
J.M. Le Roux

Adult scorpions (2.4g – 4.5 kg) were collected near Potchefstroom (26° .55’10” – 27° 10” 5”). Oxygen consumption rate (MO2) and carbon dioxide production rate (MCO2) were measured together with the metabolism of injected radioactive glucose.


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