Reagentless Biosensor Using for Stereospecific Interaction between γ-globulin andN-isobutyryl-cysteine

2013 ◽  
Vol 160 (8) ◽  
pp. B102-B106 ◽  
Author(s):  
Qian Han ◽  
Yonghua Wang ◽  
Yihan Huang ◽  
Liju Guo ◽  
Yingzi Fu
Keyword(s):  
Reagentless Biosensor

Reagentless biosensor for phenolic compounds based on tyrosinase entrapped within gelatine film

2005 ◽  
Vol 383 (7-8) ◽  
pp. 1127-1132 ◽  
Author(s):  
Nan Li ◽  
Min-Hua Xue ◽  
Hui Yao ◽  
Jun-Jie Zhu
Keyword(s):  
Phenolic Compounds ◽  
Reagentless Biosensor

scholarly journals Engineering a reagentless biosensor for single-stranded DNA to measure real-time helicase activity in Bacillus

2014 ◽  
Vol 61 ◽  
pp. 579-586 ◽  
Author(s):  
Matthew Green ◽  
Neville S. Gilhooly ◽  
Shahriar Abedeen ◽  
David J. Scott ◽  
Mark S. Dillingham ◽  
...  
Keyword(s):  
Real Time ◽  
Helicase Activity ◽  
Single Stranded Dna ◽  
Reagentless Biosensor

scholarly journals Development of a Reagentless Biosensor for Inorganic Phosphate, Applicable over a Wide Concentration Range

Biochemistry ◽  
2015 ◽  
Vol 54 (32) ◽  
pp. 5054-5062 ◽  
Author(s):  
Claudia Solscheid ◽  
Simone Kunzelmann ◽  
Colin T. Davis ◽  
Jackie L. Hunter ◽  
Annie Nofer ◽  
...  
Keyword(s):  
Inorganic Phosphate ◽  
Wide Concentration Range ◽  
Reagentless Biosensor

A Reagentless Biosensor for the Amperometric Determination of NADH

1998 ◽  
Vol 10 (8) ◽  
pp. 521-525 ◽  
Author(s):  
Serge Cosnier ◽  
Jean-Luc Décout ◽  
Marc Fontecave ◽  
Christelle Frier ◽  
Christophe Innocent
Keyword(s):  
Amperometric Determination ◽  
Reagentless Biosensor

scholarly journals Fluorescence detection of GDP in real time with the reagentless biosensor rhodamine–ParM

2011 ◽  
Vol 440 (1) ◽  
pp. 43-49 ◽  
Author(s):  
Simone Kunzelmann ◽  
Martin R. Webb
Keyword(s):  
Drug Discovery ◽  
Response Time ◽  
Real Time ◽  
Fluorescence Detection ◽  
Basic Research ◽  
Nucleotide Exchange ◽  
Gtp Hydrolysis ◽  
Fluorescence Response ◽  
Reagentless Biosensor ◽  
Fluorescence Methods

The development of novel fluorescence methods for the detection of key biomolecules is of great interest, both in basic research and in drug discovery. Particularly relevant and widespread molecules in cells are ADP and GDP, which are the products of a large number of cellular reactions, including reactions catalysed by nucleoside triphosphatases and kinases. Previously, biosensors for ADP were developed in this laboratory, based on fluorophore adducts with the bacterial actin homologue ParM. It is shown in the present study that one of these biosensors, tetramethylrhodamine–ParM, can also monitor GDP. The biosensor can be used to measure micromolar concentrations of GDP on the background of millimolar concentrations of GTP. The fluorescence response of the biosensor is fast, the response time being <0.2 s. Thus the biosensor allows real-time measurements of GTPase and GTP-dependent kinase reactions. Applications of the GDP biosensor are exemplified with two different GTPases, measuring the rates of GTP hydrolysis and nucleotide exchange.

The Study of a Disposable Reagentless Biosensor for Fast Test of Aspartate Aminotransferase

2008 ◽  
Vol 20 (10) ◽  
pp. 1135-1141 ◽  
Author(s):  
Zonghui Guo ◽  
Qingde Yang ◽  
Hongmin Liu ◽  
Chunxiu Liu ◽  
Xinxia Cai
Keyword(s):  
Aspartate Aminotransferase ◽  
Reagentless Biosensor
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