Direct Electrochemistry of Ascorbate Oxidase Self-Assembled on Au-Modified Glassy Carbon

2013 ◽  
Vol 160 (8) ◽  
pp. H414-H419 ◽  
Author(s):  
Nina Dimcheva ◽  
Totka Dodevska ◽  
Elena Horozova
Keyword(s):  
Glassy Carbon ◽  
Direct Electrochemistry ◽  
Ascorbate Oxidase ◽  
Self Assembled

Direct electrochemistry and intramolecular electron transfer of ascorbate oxidase confined on l-cysteine self-assembled gold electrode

2014 ◽  
Vol 95 ◽  
pp. 15-22 ◽  
Author(s):  
Bhushan Patil ◽  
Yoshiki Kobayashi ◽  
Shigenori Fujikawa ◽  
Takeyoshi Okajima ◽  
Lanqun Mao ◽  
...  
Keyword(s):  
Electron Transfer ◽  
Gold Electrode ◽  
Direct Electrochemistry ◽  
Ascorbate Oxidase ◽  
Intramolecular Electron Transfer ◽  
Self Assembled

Fabrication of Ascorbic Acid Biosensor Based on Coupling Polyethylene Glycol Modified Silk Fibroin Membrane onto Glassy Carbon Electrode

2016 ◽  
Vol 835 ◽  
pp. 63-70
Author(s):  
Pacharawan Ratanasongtham ◽  
Lalida Shank ◽  
Jaroon Jakmunee ◽  
Ruangsri Watanesk ◽  
Surasak Watanesk
Keyword(s):  
Ascorbic Acid ◽  
Polyethylene Glycol ◽  
Glassy Carbon Electrode ◽  
Silk Fibroin ◽  
Glassy Carbon ◽  
Phosphate Buffer ◽  
Storage Time ◽  
Initial Response ◽  
Ascorbate Oxidase ◽  
Carbon Electrode

Nowadays biosensors have been extensively used in a wide variety of applications especially in clinical works and food industry. In this work, a specific ascorbic acid (AA) biosensor was developed by immobilizing ascorbate oxidase (ASOD) on a polyethylene glycol (PEG) modified silk fibroin (SF) membrane then coupling to the glassy carbon electrode (GCE). The SF-PEG-ASOD membrane provided the highest enzyme activity in phosphate buffer at pH 5. As being the electrode, the SF-PEG-ASOD modified GCE displayed the highest response when it is operated under the condition of 0.40 mg/L of ASOD in phosphate buffer at pH 5. This biosensor provided both good linearity (r2 = 0.999 in the range of 1.0-10.0 mM) and sensitivity with short response time (26s). It also exhibited good anti-interference ability with the storage time of 5 days without changing its initial response.

scholarly journals The direct electrochemistry and bioelectrocatalysis of nitrate reductase at a gold nanoparticles/aminated graphene sheets modified glassy carbon electrode

RSC Advances ◽  
2019 ◽  
Vol 9 (64) ◽  
pp. 37207-37213 ◽  
Author(s):  
Ke Zhang ◽  
Hao Zhou ◽  
Ping Hu ◽  
Qing Lu
Keyword(s):  
Gold Nanoparticles ◽  
Nitrate Reductase ◽  
Glassy Carbon Electrode ◽  
Glassy Carbon ◽  
Direct Electrochemistry ◽  
Graphene Sheets ◽  
Carbon Electrode ◽  
Modified Glassy Carbon Electrode ◽  
And Control

The aminated graphene sheets could immobilize nitrate reductase stably and control its orientation through electrostatic attractions, achieving its direct electrochemistry.

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