Advantage of Ultra Thin Overoxidized Polypyrrole Membrane in the Design of Amperometric Biosensor

2019 ◽  
Vol 33 (8) ◽  
pp. 21-24 ◽  
Author(s):  
Catherine Debiemme-Chouvy ◽  
Gwendoline Navarro
Keyword(s):  
Amperometric Biosensor ◽  
Overoxidized Polypyrrole

scholarly journals A Crosstalk- and Interferent-Free Dual Electrode Amperometric Biosensor for the Simultaneous Determination of Choline and Phosphocholine

Sensors ◽  
2021 ◽  
Vol 21 (10) ◽  
pp. 3545
Author(s):  
Rosanna Ciriello ◽  
Antonio Guerrieri
Keyword(s):  
Simultaneous Determination ◽  
High Resolution Mass Spectrometry ◽  
Limit Of Detection ◽  
Tissue Growth ◽  
Choline Oxidase ◽  
Amperometric Biosensor ◽  
Pt Electrode ◽  
Overoxidized Polypyrrole ◽  
Resolution Mass

Choline (Ch) and phosphocholine (PCh) levels in tissues are associated to tissue growth and so to carcinogenesis. Till now, only highly sophisticated and expensive techniques like those based on NMR spectroscopy or GC/LC- high resolution mass spectrometry permitted Ch and PCh analysis but very few of them were capable of a simultaneous determination of these analytes. Thus, a never reported before amperometric biosensor for PCh analysis based on choline oxidase and alkaline phosphatase co-immobilized onto a Pt electrode by co-crosslinking has been developed. Coupling the developed biosensor with a parallel sensor but specific to Ch, a crosstalk-free dual electrode biosensor was also developed, permitting the simultaneous determination of Ch and PCh in flow injection analysis. This novel sensing device performed remarkably in terms of sensitivity, linear range, and limit of detection so to exceed in most cases the more complex analytical instrumentations. Further, electrode modification by overoxidized polypyrrole permitted the development of a fouling- and interferent-free dual electrode biosensor which appeared promising for the simultaneous determination of Ch and PCh in a real sample.

scholarly journals Assay of serum cholinesterase activity by an amperometric biosensor based on a co-crosslinked choline oxidase/overoxidized polypyrrole bilayer

The Analyst ◽  
2018 ◽  
Vol 143 (4) ◽  
pp. 920-929 ◽  
Author(s):  
Rosanna Ciriello ◽  
Sonia Lo Magro ◽  
Antonio Guerrieri
Keyword(s):  
Human Serum ◽  
Cholinesterase Activity ◽  
Platinum Electrode ◽  
Choline Oxidase ◽  
Amperometric Biosensor ◽  
Serum Cholinesterase ◽  
Electrochemical Assay ◽  
Overoxidized Polypyrrole ◽  
Serum Cholinesterase Activity

Based on choline oxidase immobilized by co-crosslinking on an overoxidised polypyrrole modified platinum electrode, a novel electrochemical assay for cholinesterase activity in human serum was developed.

Instrumentation system for amperometric biosensor

2011 ◽  
Vol 1 (10) ◽  
pp. 49-51
Author(s):  
Chethan .G Chethan .G ◽  
◽  
Saurav Pratap Singh ◽  
Dr. Padmaja .K.V Dr. Padmaja .K.V ◽  
Dr. Prasanna kumar .S.C Dr. Prasanna kumar .S.C
Keyword(s):  
Amperometric Biosensor

An antibody-based amperometric biosensor for 20S proteasome activity and inhibitor screening

The Analyst ◽  
2021 ◽  
Author(s):  
Madalina M. Barsan ◽  
Victor C. Diculescu
Keyword(s):  
Proteasome Activity ◽  
Electrochemical Methods ◽  
Amperometric Biosensor ◽  
20S Proteasome ◽  
Specific Interactions ◽  
Inhibitor Screening

The 20S proteasome is immobilized through specific interactions with antibodies and its activity is evaluated by electrochemical methods.

Quantification of l-lysine in cheese by a novel amperometric biosensor

2015 ◽  
Vol 169 ◽  
pp. 13-19 ◽  
Author(s):  
Rosanna Ciriello ◽  
Tommaso R.I. Cataldi ◽  
Fabiana Crispo ◽  
Antonio Guerrieri
Keyword(s):  
Amperometric Biosensor

Development of a new amperometric biosensor based on polyphenoloxidase and polyethersulphone membrane

2001 ◽  
Vol 73 (12) ◽  
pp. 1993-1999 ◽  
Author(s):  
P. V. Climent ◽  
M. L. M. Serralheiro ◽  
M. J. F. Rebelo
Keyword(s):  
Phenolic Compounds ◽  
Response Time ◽  
Enzyme Immobilization ◽  
Platinum Electrode ◽  
Enzymatic Catalysis ◽  
Amperometric Biosensor ◽  
Catecholase Activity

An amperometric biosensor based on the enzyme polyphenoloxidase (PPO), which makes the bioelectrocatalysis of phenolic compounds, was developed and optimized using cathecol as substrate. Polyethersulphone membranes were used for enzyme immobilization. Polyphenoloxidase oxidizes monophenols (cresolase activity) and diphenols (catecholase activity) into the corresponding o-quinones; the o-quinones formed in the enzymatic catalysis are then reduced back to cathecol at ­200 mV (vs. Ag, AgCl) at a platinum electrode. The polyphenoloxidase immobilized was from commercial origin or extracted from mushrooms. p-Cresol and phenol substrates were also tested. Reproducibility, response time, linearity, sensitivity, and stability of the biosensor were studied.

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