Nano Gold / Phytic Acid Layer-by-layer Assembly for Fabrication of Horseradish Peroxidase Biosensor

2019 ◽  
Vol 16 (11) ◽  
pp. 491-496
Author(s):  
Xiaoling Ma ◽  
Yaqin Zheng ◽  
Guoping Duan ◽  
Zongrang Zhang ◽  
Haifeng Yang
2010 ◽  
Vol 157 (1) ◽  
pp. K5 ◽  
Author(s):  
Yao Wang ◽  
Xiaoling Ma ◽  
Ying Wen ◽  
Yaqing Zheng ◽  
Guoping Duan ◽  
...  

2011 ◽  
Vol 217-218 ◽  
pp. 1793-1796 ◽  
Author(s):  
Li Juan Gao ◽  
Ning Gan ◽  
Fu Tao Hu ◽  
Yu Ting Cao ◽  
Lei Zheng

This paper introduces a novel amperometric immunosensor consisting of hemin (Fe(III)-protoporphyrinIX) layers formed by layer-by-layer assembly for determination of clenbuterol (CLB). Layer-by-layer assembly of hemin with Poly(allylamine hydrochloride) (PAH) is achieved on the electrode surface which is modified by single-wall carbon nanotubes (SWCNTs) based on the electrostatic attraction between negatively charged hemin and positively charged Poly(allylamine hydrochl- oride). The modified electrodes were characterized by cyclic voltammetry (CV), scanning electron microscope (SEM), and X-ray photo electron spectrum (XPS). The decrease of current results from the increasing concentration of CLB antigen, which leads to a linear decrease in CLB antigen concentration ranges from 0.5 ~ 10 ng/mL with the detection limit of 0.2 ng/mL (3σ).


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