Steady‐State Characteristics of Oxygen Concentration Cell Sensors Subjected to Nonequilibrium Gas Mixtures

1981 ◽  
Vol 128 (2) ◽  
pp. 294-300 ◽  
Author(s):  
J. E. Anderson ◽  
Y. B. Graves
Keyword(s):  
Steady State ◽  
Oxygen Concentration ◽  
Gas Mixtures ◽  
Concentration Cell ◽  
Nonequilibrium Gas

Voltage Step Characteristics of Oxygen Concentration Cell Sensors for Nonequilibrium Gas Mixtures

1988 ◽  
Vol 135 (7) ◽  
pp. 1686-1691 ◽  
Author(s):  
Keiichi Saji ◽  
Haruyoshi Kondo ◽  
Takashi Takeuchi ◽  
Isemi Igarashi
Keyword(s):  
Oxygen Concentration ◽  
Gas Mixtures ◽  
Voltage Step ◽  
Concentration Cell ◽  
Nonequilibrium Gas

Kinetics of the cytochrome c oxidase and reductase reactions in energized and de-energized mitochondria

1977 ◽  
Vol 55 (7) ◽  
pp. 706-713 ◽  
Author(s):  
Lars Chr. Petersen ◽  
Hans Degn ◽  
Peter Nicholls
Keyword(s):  
Steady State ◽  
Cytochrome C ◽  
Cytochrome C Oxidase ◽  
Oxygen Concentration ◽  
Respiration Rate ◽  
Redox State ◽  
Rat Liver Mitochondria ◽  
Excess Oxygen ◽  
Succinate Oxidation ◽  
State Reduction

1. Coupled, cytochrome-c-depleted ('stripped') rat liver mitochondria reducing oxygen in the presence of exogenous cytochrome c, with succinate or ascorbate as substrates, show marked declines in the steady-state reduction of cytochrome c in excess oxygen on addition of uncouplers. Calculated ratios of maximal turnover in the uncoupled state and in the energized state for the cytochrome c oxidase (EC 1.9.3.1) reaction lie between 3 and 6, as obtained with reconstituted oxidase-containing vesicles. The succinate-cytochrome c reductase activity in such mitochondria shows a smaller response to uncoupler than that of the oxidase.2. The respiration rates of uncoupled mitochondria oxidizing ascorbate in the presence of added cytochrome c follow a Michaelis–Menten relationship with respect to oxygen concentration, in accordance with the pattern found previously with the solubilized oxidase. But succinate oxidation tends to give nonlinear concave-upward double-reciprocal plots of respiration rate against oxygen concentration, in accordance with the pattern found previously with intact uncoupled mitochondria.3. From simultaneous measurements of cytochrome c steady-state reduction, respiration rate, and oxygen concentration during succinate oxidation under uncoupled conditions it is found that at full reduction of cytochrome c, apparent Km for oxygen is 0.9 μM and the maximal oxidase (aa3) turnover is 400 s−1 (pH 7.4, 30 °C).4. The redox state of cytochrome c in uncoupled systems reflects a simple steady state; the redox state of cytochrome c in energized systems tends towards an equilibrium condition with the terminal cytochrome a3, whose apparent potential under these conditions is more negative than that of cytochrome c.

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