A Functional Description of ANALYZE: A Computer-Assisted Analysis System for Linear Programming Models

1983 ◽  
Vol 9 (1) ◽  
pp. 18-56 ◽  
Author(s):  
Harvey Greenberg
Keyword(s):  
Linear Programming ◽  
Programming Models ◽  
Computer Assisted ◽  
Functional Description ◽  
Analysis System ◽  
Assisted Analysis

Optimization of a continuous real-time computerized semen analysis system for ram sperm motility assessment, and evaluation of four methods of semen preparation

1996 ◽  
Vol 8 (2) ◽  
pp. 219 ◽  
Author(s):  
WV Holt ◽  
MJ Palomo
Keyword(s):  
Real Time ◽  
Semen Analysis ◽  
Computer Assisted ◽  
Assessment And Evaluation ◽  
Analysis System ◽  
Set Up ◽  
Assisted Analysis ◽  
Ram Sperm ◽  
Preparation Techniques

Sampling conditions that affect the biological validity of computer-assisted analysis of ram sperm motion were examined using a continuous real-time computerized semen analysis system (Hobson Sperm Tracker). Search radius (SR, 10 settings) and minimum track point (MTP, 10 settings) were varied factorially to evaluate their effects on the inclusion of sperm subpopulations within derived datasets. Low SR (< 12 microns) or high MTP values (> 26 frames) precluded measurements of rapidly moving cells, whereas high SR (> 17 microns) and low MTP settings (< 22 frames) led to erroneous tracking and poor data quality. Suitable settings for these set-up parameters were derived and tested for their biological consistency with four methods of preparing ram semen for computerized analysis. The preparation techniques tested were: centrifugation through sucrose-based Ficoll and Percoll media, a swim-up technique and simple dilution in Tyrode's media. The 'selective' Percoll and swim-up methods generated sperm populations with significantly higher linear velocities and a lower tendency to deviate from linear trajectories than from either the Ficoll method or dilution technique. Deleterious effects of centrifugation were evident, particularly on sperm survival in vitro over several hours. It is concluded that computer-assisted semen analysis provides useful information about the behaviour of ram spermatozoa in vitro, but the measurement conditions must be defined carefully.

scholarly journals Computer analysis of double labeling: ultrastructural distribution of vasoactive intestinal peptide and transferrin in human colon cancer cells.

1990 ◽  
Vol 38 (9) ◽  
pp. 1257-1266 ◽  
Author(s):  
C Lefevre ◽  
H H Phan ◽  
A Anteunis ◽  
G Rosselin
Keyword(s):  
Vasoactive Intestinal Peptide ◽  
Human Colon ◽  
Endocytic Pathway ◽  
Computer Assisted ◽  
Human Colon Cancer ◽  
Double Labeling ◽  
Colon Carcinoma Cells ◽  
Analysis System ◽  
Assisted Analysis ◽  
Endosomal Vesicles

Transferrin (Tf) and vasoactive intestinal peptide (VIP) were labeled with horseradish peroxidase (HRP) and 125I, respectively. To determine whether two simultaneously incubated ligands are conveyed by the same population of endosomal vesicles in human colon carcinoma cells (HT-29), we used an analysis system derived from the cross-fire method for quantitation of autoradiographic data. This system permitted the collection of data and the statistical calculations required by the double labeling of the cells. HRP-labeled Tf organelles were chosen as reference structures of the endosomal apparatus and taken as the conventional source of the radiolabeling. Our data established from the co-localization hypothesis strongly suggest that after a 30-sec (T 1/2) and a 10-min (T10) internalization at 37 degrees C, VIP and Tf share in major part the same endocytic pathway and even the recycling route to the cell surface. At T10, most of the radiolabeling was located inside the tubulovesicular network, and we also detected slight radiolabeling inside the vesicles recycling Tf. The number of double-labeled endosomes involved in ligand traffic were advantageously observed with our computer-assisted analysis system.

DINAS: A computer-assisted analysis system for multiobjective transshipment problems with facility location

1992 ◽  
Vol 19 (7) ◽  
pp. 637-647 ◽  
Author(s):  
Włodzimierz Ogryczak ◽  
Krzysztof Studziński ◽  
Krystian Zorychta
Keyword(s):  
Facility Location ◽  
Computer Assisted ◽  
Analysis System ◽  
Assisted Analysis

Computer-Assisted Analysis of Multi-Color Confocal Microscopic Datasets: Automated Light Microscopic Discrimination of Synaptic Relationships

1996 ◽  
Vol 54 ◽  
pp. 284-285
Author(s):  
M Wessendorf ◽  
A Beuning ◽  
D Cameron ◽  
J Williams ◽  
C Knox
Keyword(s):  
Nerve Fibers ◽  
Confocal Scanning Laser Microscopy ◽  
Computer Assisted ◽  
Nerve Terminals ◽  
Neuronal Somata ◽  
Scanning Laser Microscopy ◽  
Confocal Scanning ◽  
Entire Cell ◽  
Confocal Scanning Laser ◽  
Assisted Analysis

Multi-color confocal scanning-laser microscopy (CSLM) allows examination of the relationships between neuronal somata and the nerve fibers surrounding them at sub-micron resolution in x,y, and z. Given these properties, it should be possible to use multi-color CSLM to identify relationships that might be synapses and eliminate those that are clearly too distant to be synapses. In previous studies of this type, pairs of images (e.g., red and green images for tissue stained with rhodamine and fluorescein) have been merged and examined for nerve terminals that appose a stained cell (see, for instance, Mason et al.). The above method suffers from two disadvantages, though. First, although it is possible to recognize appositions in which the varicosity abuts the cell in the x or y axes, it is more difficult to recognize them if the apposition is oriented at all in the z-axis—e.g., if the varicosity lies above or below the neuron rather than next to it. Second, using this method to identify potential appositions over an entire cell is time-consuming and tedious.

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