Comparison of 3 Methods for in Vitro Isolation of Human Ovarian Granulosa Cell

2018 ◽  
Author(s):  
Hua Zhou ◽  
Zhixu He ◽  
Shuyun Zhao
Keyword(s):  
Granulosa Cell ◽  
Ovarian Granulosa Cell

scholarly journals Effect of the Methoxychlor Metabolite HPTE on the Rat Ovarian Granulosa Cell Transcriptome In Vitro

2009 ◽  
Vol 110 (1) ◽  
pp. 95-106 ◽  
Author(s):  
Craig N. Harvey ◽  
Mahmoud Esmail ◽  
Qi Wang ◽  
Andrew I. Brooks ◽  
Rob Zachow ◽  
...  
Keyword(s):  
Granulosa Cell ◽  
Ovarian Granulosa Cell ◽  
Cell Transcriptome

In-vitro role of leptin on ovarian granulosa cell steroidogenesis.

2001 ◽  
Vol 76 (3) ◽  
pp. S162
Author(s):  
F Dincsahin ◽  
O Taskin ◽  
E Erturan ◽  
S Kursun ◽  
C Ispahi ◽  
...  
Keyword(s):  
Granulosa Cell ◽  
Ovarian Granulosa Cell

scholarly journals Effects of benzene, quercetin, and their combination on porcine ovarian cell proliferation, apoptosis, and hormone release

2019 ◽  
Vol 62 (1) ◽  
pp. 345-351 ◽  
Author(s):  
Adam Tarko ◽  
Aneta Štochmal'ová ◽  
Katarína Jedličková ◽  
Sandra Hrabovszká ◽  
Adriana Vachanová ◽  
...  
Keyword(s):  
Granulosa Cell ◽  
Protective Action ◽  
Inhibitory Effect ◽  
Hormone Release ◽  
Ovarian Cell ◽  
Cell Functions ◽  
Ovarian Granulosa Cell ◽  
Oxytocin Release ◽  
Prostaglandin F

Abstract. We hypothesized that the environmental contaminant benzene and the plant antioxidant quercetin may affect ovarian cell functions and that quercetin could offer protection against the adverse effects of benzene. This study aimed to examine the action of benzene, quercetin, and their combination on porcine ovarian granulosa cell functions. We elucidated the effects of benzene (20 µg mL−1), quercetin (at the doses 0, 1, 10, 100 µg mL−1), and their combination on ovarian granulosa cell functions (proliferation, apoptosis, and hormone release) in vitro using immunocytochemistry and enzyme immunoassay respectively. Benzene alone stimulated proliferation, apoptosis, and oxytocin release and inhibited progesterone and prostaglandin F release. Quercetin alone inhibited proliferation, apoptosis, and stimulated oxytocin release but did not affect progesterone and prostaglandin F release. When used in combination with benzene, quercetin promoted the inhibitory effect of benzene on progesterone release. Overall, these data suggest that benzene and quercetin have direct stimulatory and inhibitory effects, respectively, on basic ovarian functions. Moreover, no protective action of quercetin against the effects of benzene was found. Rather, it was found to enhance the effect of benzene on progesterone release. Therefore, quercetin cannot be considered for preventing or mitigating the effects of benzene on reproductive processes.

scholarly journals Downregulating lncRNA NEAT1 induces proliferation and represses apoptosis of ovarian granulosa cells in polycystic ovary syndrome via microRNA-381/IGF1 axis

2021 ◽  
Vol 28 (1) ◽  
Author(s):  
Jingran Zhen ◽  
Jiangli Li ◽  
Xia Li ◽  
Xue Wang ◽  
Yaling Xiao ◽  
...  
Keyword(s):  
Granulosa Cell ◽  
Granulosa Cells ◽  
Cell Apoptosis ◽  
Polycystic Ovary ◽  
Pathological Changes ◽  
Ovary Syndrome ◽  
Ovarian Granulosa Cells ◽  
Ovarian Granulosa Cell ◽  
Igf1 Expression

Abstract Objective Researchers have revealed the combined functions of long noncoding RNAs (lncRNAs) and microRNA (miRNAs) in polycystic ovary syndrome (PCOS). This study aimed to understand the role of nuclear-enriched abundant transcript 1 (NEAT1) and miR-381 involving insulin-like growth factor 1 (IGF1) in PCOS. Methods PCOS rat model was established by dehydroepiandrosterone induction. NEAT1, miR-381 and IGF1 expression in ovarian granulosa cells of PCOS patients and ovarian tissues of PCOS rats were tested. Bioinformatics website and dual luciferase reporter gene assay were utilized to verify the relationship between NEAT1 and miR-381 and that between miR-381 and IGF1. Levels of sex hormone, pathological changes and ovarian granulosa cell apoptosis in ovarian tissues of PCOS rats were detected. Ovarian granulosa cell proliferation and apoptosis were analyzed in vitro. Results NEAT1 and IGF1 expression increased while miR-381 expression decreased in the ovarian granulosa cells of patients with PCOS and the ovarian tissues of PCOS rats. In in vivo experiments, interference with NEAT1 improved the levels of sex hormones, alleviated pathological changes and suppressed ovarian granulosa cell apoptosis in the ovarian tissues of PCOS rats. In in vitro cell experiments, interference with NEAT1 suppressed apoptosis and enhanced cell proliferation of ovarian granulosa cells. NEAT1 interference-mediated effect would be reversed by up-regulating miR-381. NEAT1 acted as a ceRNA to adsorb miR-381 to target IGF1. Overexpression of IGF1 reversed the inhibitory effect of miR-381 on ovarian granulosa cell apoptosis. Conclusion Interference with NEAT1 increases miR-381 and reduces IGF1 levels, effectively improving the levels of sex hormones and reducing the pathological damage of ovarian tissue in rats with PCOS.

Study on the effect of antiprogestational agents on goat ovarian granulosa cell estradiol production in vitro

1986 ◽  
Vol 25 ◽  
pp. 132
Author(s):  
S.M. Kharbanda ◽  
V. Band ◽  
K. Murugesan ◽  
U. Verma ◽  
A. Farooq
Keyword(s):  
Granulosa Cell ◽  
Ovarian Granulosa Cell

Evidence for Existence of Immunoglobulins That Block Ovarian Granulosa Cell Growth in Vitro. A Putative Role in Resistant Ovary Syndrome

1992 ◽  
Vol 47 (1) ◽  
pp. 55-57
Author(s):  
MIRJAM M. VAN WEISSENBRUCH ◽  
ANNEMIEKE HOEK ◽  
INGRID VAN VLIET-BLEEKER ◽  
JOOP SCHOEMAKER ◽  
HEMMO DREXHAGE
Keyword(s):  
Cell Growth ◽  
Granulosa Cell ◽  
Ovary Syndrome ◽  
Putative Role ◽  
Ovarian Granulosa Cell ◽  
Resistant Ovary Syndrome

scholarly journals Menoprogen, a TCM Herbal Formula for Menopause, Increases Endogenous E2in an Aged Rat Model of Menopause by Reducing Ovarian Granulosa Cell Apoptosis

2016 ◽  
Vol 2016 ◽  
pp. 1-12
Author(s):  
Yu Li ◽  
Hong Ma ◽  
Ye Lu ◽  
B. J. Tan ◽  
L. Xu ◽  
...  
Keyword(s):  
Granulosa Cell ◽  
Rat Model ◽  
Secretory Granules ◽  
Female Rats ◽  
Estradiol Valerate ◽  
Intragastric Administration ◽  
Aged Rat ◽  
Ovarian Granulosa Cell

The effect of Menoprogen (MPG) on ovarian granulosa cell (GC) apoptosis was investigated in vitro and in vivo in an aged rat model of menopause. Intragastric administration of Menoprogen or estradiol valerate to 14-month-old senile female rats for eight weeks increased plasmaE2levels, as well as the weight of both ovarian and uterine tissues. Flow cytometric (FCM) analysis of isolated GCs from MPG-treated aged rats showed reductions in theG0/G1ratio and apoptotic peaks. Isolated GCs also exhibited an increase in cell size and the number of cytoplastic organelles and intracellular gap junctions, the reappearance of secretory granules, and a lack of apoptotic bodies as determined by TEM. Results from a TdT-mediated dUTP nick end-labeling (TUNEL) assay revealed a reduction in TUNEL-positive GCs after MPG treatment. Immunohistochemical analysis showed a downregulation of proapoptotic Bax proteins and an upregulation of antiapoptotic Bcl-2 proteins. The addition of MPG-medicated serum to the media of cultured GCs also reduced cadmium chloride-induced apoptosis and downregulated caspase-3 protein expression. This work demonstrates that Menoprogen inhibits GC apoptosis in aged female rats and thereby increasesE2production. This represents a novel mechanism of action for this herbal medicine in the treatment of menopausal symptoms.

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