scholarly journals Infrared Spectra and Secondary Structure of Deoxyribonucleic Acid

1961 ◽  
Vol 17 ◽  
pp. 99-107 ◽  
Author(s):  
Masamichi Tsuboi
1971 ◽  
Vol 19 (12) ◽  
pp. 761-765 ◽  
Author(s):  
MANUEL DIAZ ◽  
JOSE HIERRO ◽  
GRACIELA DEMICHELI DE DIAZ

A new method is proposed to study the secondary structure of deoxyribonucleic acid (DNA) in situ in fixed chromatin. It is based on acriflavine staining and on differentiation with a nitrous acid solution of the fixed cytologic preparation. The presence of green fluorescence after this treatment is regarded as indicative of double stranded DNA. Experiments are described with DNA-acriflavine mixtures in solution, DNA-agar models and cytologic preparations submitted to different pretreatments. The feasibility and limitations of the method are discussed in the light of the results reported upon.


2000 ◽  
Vol 285 (1) ◽  
pp. 33-49 ◽  
Author(s):  
Ganesh Vedantham ◽  
H.Gerald Sparks ◽  
Samir U. Sane ◽  
Stelios Tzannis ◽  
Todd M. Przybycien

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