A Porphyrin Molecularly Imprinted Biomimetic Electrochemical Sensor Based on Gold Nanoparticles and Carboxyl Graphene Composite for the Highly Efficient Detection of Methyl Parathion

NANO ◽  
2017 ◽  
Vol 12 (04) ◽  
pp. 1750046 ◽  
Author(s):  
Bin He ◽  
Ya-Li Mao ◽  
Ya Zhang ◽  
Wei Yin ◽  
Chang-Jun Hou ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Methyl Parathion ◽  
Dynamic Range ◽  
Limit Of Detection ◽  
Differential Pulse ◽  
Molecularly Imprinted ◽  
Zinc Porphyrin ◽  
Efficient Detection ◽  
Novel Strategy ◽  
Biomimetic Sensor

A highly sensitive and selective biomimetic sensor based on zinc porphyrin molecularly imprinted Polymer microspheres (MIPMs), gold nanoparticles (AuNPs) and carboxyl graphene (CG) nanomaterials was successfully developed for direct electrochemical detection of methyl parathion (MP). The novel strategy emphasized the fabrication of a porphyrin zinc-based sensor via attaching MIPMs on AuNPs/CG nanocomposites. MIPMs was prepared by free radical polymerization using MP as the template, Zinc porphyrin as the functional monomer, ethylene glycol dimethacrylate (EGDMA) as the cross-linking reagent and azobisisobutyronitrile (AIBN) as the initiator. The introduction of AuNPs/CG significantly increased the effective electrode area, and amplified the sensor signal. The modified electrode was characterized by cyclic voltammetry (CV) and differential pulse voltammetry (DPV). The parameters of the detection process were also optimized. The biomimetic sensor exhibits a much wider linear dynamic range between 1.0[Formula: see text][Formula: see text][Formula: see text]10[Formula: see text][Formula: see text]mol L[Formula: see text] and 8.0[Formula: see text][Formula: see text][Formula: see text]10[Formula: see text][Formula: see text]mol L[Formula: see text] and the limit of detection (LOD) down to 3.16[Formula: see text][Formula: see text][Formula: see text]10[Formula: see text][Formula: see text]mol L[Formula: see text] based on S/N [Formula: see text] 3. The sensor had good reproducibility, stability and selectivity for MP detection. The developed sensor was successfully employed for the detection of MP in real samples.

Gold Nanoparticle Based Electrochemical Immunosensor for Detection of T3 Hormone

2020 ◽  
Vol 20 (10) ◽  
pp. 6057-6062
Author(s):  
Rahul Saxena ◽  
H. Fouad ◽  
Sudha Srivastava
Keyword(s):  
Gold Nanoparticles ◽  
Cyclic Voltammetry ◽  
Electrode Surface ◽  
Dynamic Range ◽  
Limit Of Detection ◽  
Differential Pulse ◽  
Electrochemical Immunosensor ◽  
Antigen Concentration ◽  
Antigen Present ◽  
Pulse Voltammetry

We report a nanoparticles based electrochemical immunosensor to detect and quantify triiodothyronine (T3) hormone. Immunosensor developed using gold nanoparticles and anti-T3 antibody, was employed for quantification of T3 antigen using cyclic voltammetry (CV) and differential pulse voltammetry (DPV) technique. The electrochemical response of the developed immunosensor correlates well with the amount of antigen present in the sample. With increase in antigen concentration the immunocomplex formation on electrode surface increases and hence redox current decreases. The immunosensor shows a lower limit of detection of 1 pg/mL and dynamic range from 1 to 500 pg/mL. Sensitivity of the immunosensor was found to be 29.81 μA/pg/mL/cm2.

scholarly journals Supramolecular assemblies of carbon nanocoils and tetraphenylporphyrin derivatives for sensing of catechol and hydroquinone in aqueous solution

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Syeda Aqsa Batool Bukhari ◽  
Habib Nasir ◽  
Lujun Pan ◽  
Mehroz Tasawar ◽  
Manzar Sohail ◽  
...  
Keyword(s):  
Electrochemical Impedance ◽  
Limit Of Detection ◽  
Linear Trend ◽  
Differential Pulse ◽  
Peak Potential ◽  
Limit Of Quantification ◽  
Efficient Detection ◽  
Good Repeatability ◽  
Ft Ir ◽  
Pulse Voltammetry

AbstractNon-enzymatic electrochemical detection of catechol (CC) and hydroquinone (HQ), the xenobiotic pollutants, was carried out at the surface of novel carbon nanocoils/zinc-tetraphenylporphyrin (CNCs/Zn-TPP) nanocomposite supported on glassy carbon electrode. The synergistic effect of chemoresponsive activity of Zn-TPP and a large surface area and electron transfer ability of CNCs lead to efficient detection of CC and HQ. The nanocomposite was characterized by using FT-IR, UV/vis. spectrophotometer, SEM and energy dispersive X-ray spectroscopy (EDS). Cyclic voltammetry, differential pulse voltammetry (DPV) and electrochemical impedance spectroscopy were used for the electrochemical studies. CNCs/Zn-TPP/GCE nanosensor displayed a limit of detection (LOD), limit of quantification (LOQ) and sensitivity for catechol as 0.9 µM, 3.1 µM and 0.48 µA µM−1 cm−2, respectively in a concentration range of 25–1500 µM. Similarly, a linear trend in the concentration of hydroquinone detection was observed between 25 and 1500 µM with an LOD, LOQ and sensitivity of 1.5 µM, 5.1 µM and 0.35 µA µM−1 cm−2, respectively. DPV of binary mixture pictured well resolved peaks with anodic peak potential difference, ∆Epa(CC-HQ), of 110 mV showing efficient sensing of CC and HQ. The developed nanosensor exhibits stability for up to 30 days, better selectivity and good repeatability for eight measurements (4.5% for CC and 5.4% for HQ).

scholarly journals Quantification of DNA by a Thermal-Durable Biosensor Modified with Conductive Poly(3,4-ethylenedioxythiophene)

Sensors ◽  
2018 ◽  
Vol 18 (11) ◽  
pp. 3684 ◽  
Author(s):  
Yesong Gu ◽  
Po-Yuan Tseng ◽  
Xiang Bi ◽  
Jason Yang
Keyword(s):  
Gold Nanoparticles ◽  
Limit Of Detection ◽  
Differential Pulse ◽  
Complementary Sequence ◽  
Signal Decay ◽  
Target Dna ◽  
Hybridization Efficiency ◽  
Sh Group ◽  
Polymerase Chain ◽  
Or Gene

The general clinical procedure for viral DNA detection or gene mutation diagnosis following polymerase chain reaction (PCR) often involves gel electrophoresis and DNA sequencing, which is usually time-consuming. In this study, we have proposed a facile strategy to construct a DNA biosensor, in which the platinum electrode was modified with a dual-film of electrochemically synthesized poly(3,4-ethylenedioxythiophene) (PEDOT) resulting in immobilized gold nanoparticles, with the gold nanoparticles easily immobilized in a uniform distribution. The DNA probe labeled with a SH group was then assembled to the fabricated electrode and employed to capture the target DNA based on the complementary sequence. The hybridization efficiency was evaluated with differential pulse voltammetry (DPV) in the presence of daunorubicin hydrochloride. Our results demonstrated that the peak current in DPV exhibited a linear correlation the concentration of target DNA that was complementary to the probe DNA. Moreover, the electrode could be reused by heating denaturation and re-hybridization, which only brought slight signal decay. In addition, the addition of the oxidized form of nicotinamide adenine dinucleotide (NAD+) could dramatically enhance the sensitivity by more than 5.45-fold, and the limit-of-detection reached about 100 pM.

Electrochemical Detection of Fenthion Insecticide in Olive Oils by a Sensitive Non-Enzymatic Biomimetic Sensor Enhanced with Metal Nanoparticles

2021 ◽  
Vol 5 (1) ◽  
pp. 64
Author(s):  
Youssra Aghoutane ◽  
Nezha El Bari ◽  
Zoubida Laghrari ◽  
Benachir Bouchikhi
Keyword(s):  
Electrochemical Impedance ◽  
Differential Pulse ◽  
Organophosphate Insecticide ◽  
Imprinted Polymer ◽  
High Recovery ◽  
Molecularly Imprinted ◽  
Olive Oils ◽  
Pulse Voltammetry ◽  
Biomimetic Sensor

Fenthion, an organophosphate insecticide, is a cholinesterase inhibitor and is highly toxic. An electrochemical sensor based on molecularly imprinted polymer is developed here for its detection. For this purpose, 2-aminothiophenol mixed with gold nanoparticles was immobilized on screen-printed gold electrodes. The FEN pattern was then fixed before being covered with 2-aminothiophenol. Cyclic voltammetry, differential pulse voltammetry and electrochemical impedance spectroscopy methods were used for the electrochemical characterization. The low detection limit was 0.05 mg/Kg over a range of 0.01–17.3 µg/mL. The sensor was successfully applied for the determination of FEN in olive oil samples with high recovery values.

scholarly journals A chitosan gold nanoparticles molecularly imprinted polymer based ciprofloxacin sensor

RSC Advances ◽  
2020 ◽  
Vol 10 (22) ◽  
pp. 12823-12832 ◽  
Author(s):  
Sandeep G. Surya ◽  
Shahjadi Khatoon ◽  
Abdellatif Ait Lahcen ◽  
An T. H. Nguyen ◽  
Boris B. Dzantiev ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Molecularly Imprinted Polymer ◽  
Imprinted Polymer ◽  
Molecularly Imprinted ◽  
Biomimetic Sensor

In this work, we present a novel study on the development of an electrochemical biomimetic sensor to detect the ciprofloxacin (CIP) antibiotic.

scholarly journals Sensitive and Selective Electrochemical Detection of Epirubicin as Anticancer Drug Based on Nickel Ferrite Decorated with Gold Nanoparticles

Micromachines ◽  
2021 ◽  
Vol 12 (11) ◽  
pp. 1334
Author(s):  
Mohammad Mehmandoust ◽  
Nevin Erk ◽  
Ceren Karaman ◽  
Fatemeh Karimi ◽  
Sadegh Salmanpour
Keyword(s):  
Electron Microscopy ◽  
Gold Nanoparticles ◽  
Nickel Ferrite ◽  
Electrochemical Impedance ◽  
Limit Of Detection ◽  
Differential Pulse ◽  
X Ray ◽  
Transmission Electron ◽  
The Stability ◽  
Electrochemical Electrode

The accurate and precise monitoring of epirubicin (EPR), one of the most widely used anticancer drugs, is significant for human and environmental health. In this context, we developed a highly sensitive electrochemical electrode for EPR detection based on nickel ferrite decorated with gold nanoparticles (Au@NiFe2O4) on the screen-printed electrode (SPE). Various spectral characteristic methods such as Fourier transform infrared spectra (FT-IR), X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM), ultraviolet-visible spectroscopy (UV-Vis), energy-dispersive X-ray spectroscopy (EDX) and electrochemical impedance spectroscopy (EIS) were used to investigate the surface morphology and structure of the synthesized Au@NiFe2O4 nanocomposite. The novel decorated electrode exhibited a high electrocatalytic activity toward the electrooxidation of EPR, and a nanomolar limit of detection (5.3 nM) was estimated using differential pulse voltammetry (DPV) with linear concentration ranges from 0.01 to 0.7 and 0.7 to 3.6 µM. The stability, selectivity, repeatability reproducibility and reusability, with a very low electrode response detection limit, make it very appropriate for determining trace amounts of EPR in pharmaceutical and clinical preparations.

Colorimetric Sensing of Methyl Parathion Using Nanozymatic Activity of Gold Nanoparticles

2020 ◽  
Vol 12 (2) ◽  
pp. 232-241
Author(s):  
Anjana Pandey ◽  
Saumya Srivastava ◽  
Gayatri ◽  
Priya Rai ◽  
Ashutosh Pandey
Keyword(s):  
Gold Nanoparticles ◽  
Methyl Parathion ◽  
Limit Of Detection ◽  
Colorimetric Assay ◽  
Cost Effective ◽  
Colorimetric Sensing ◽  
Electron Transfer Mechanism ◽  
Au Nps ◽  
New Approach ◽  
Cost Effective Technique

In this study, we have used new approach for detection of the methyl parathion residues i.e., by inhibiting half of the peroxidase-like activity of gold nanoparticles. We have investigated the peroxidase like activity of gold nanoparticles by colorimetric assay and optimized the pH, temperature, incubation time and different concentrations of H2O2 by using TMB as substrate as well as organophosphates effect on their enzyme mimetic activity. Kinetic study of gold nanoparticles has shown better catalytic activity than horseradish peroxidase at pH 3.5. The peroxidase substrate TMB (3,3,5,5-tetramethylbenzidine) can be oxidized by H2O2 by the enzymatic action of the gold nanoparticles resulting in a blue-coloured product, oxidized TMB. The principle involved behind the inhibition of enzymatic activity of nanozyme is due to hindrance of electron transfer mechanism TMB-H2O2-Au NPs system by the methyl parathion. The peroxidase activity is selectively reduced with increasing methyl parathion concentration. This sensing method has lowest limit of detection of 78.95 nM. This study can be used for development of sensitive and cost effective technique for sensing of harmful pesticides.

scholarly journals Novel Competitive Voltammetric Aptasensor Based on Electrospun Carbon Nanofibers-Gold Nanoparticles Modified Graphite Electrode for Salmonella enterica serovar Detection

2020 ◽  
Vol 11 (2) ◽  
pp. 8702-8715
Keyword(s):  
Gold Nanoparticles ◽  
Carbon Nanofibers ◽  
Electrochemical Detection ◽  
Salmonella Enterica ◽  
Electrochemical Impedance ◽  
Graphite Electrode ◽  
Limit Of Detection ◽  
Differential Pulse ◽  
Pencil Graphite Electrode ◽  
Experimental Conditions

Salmonella enterica is considered one of the most common bacterial agent causes of acute gastroenteritis and foodborne illness in humans worldwide. Antibiotic-resistant is considered as a major problem in Salmonella enterica Serovar. This study introduces a new simple and sensitive aptasensor based on chitosan (Chi)-electrospun carbon nanofibers (CNF) /gold nanoparticles (GNPs) decorated pencil graphite electrode (GE) as a novel platform for electrochemical detection of Salmonella enterica Serovar. A Salmonella-specific recognition aptamer ssDNA sequence was used in the development of this voltammetric biosensor. Electrochemical behaviors of electrodes; unmodified GE, CNF-Chi/GE, GNPs/CNF-Chi/GE, GNPs/CNF-Chi/GEs linked with the aptamer were studied by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). After the optimization of experimental conditions (e.g., CNF concentration, pH, and incubation time), electrochemical detection of Salmonella was performed via differential pulse voltammetry (DPV) in methylene blue solution. The designed aptasensor exhibited a linear range of 10 to 105 (CFU/mL) with the limit of detection (LOD) 1.223 (Cfu/mL) for Salmonella. This aptasensor displayed excellent selectivity and remarkable sensitivity in terms of the detection of Salmonella enterica even in the real samples as compared to the polymerase chain reaction (PCR) technique. The constructed aptasensor is a highly sensitive sensor for the detection of Salmonella enterica and also can be tailored for various other targets.

scholarly journals A Novel Method for Extraction of Galegine by Molecularly Imprinted Polymer (MIP) Technique Reinforced with Graphene Oxide and Its Evaluation Using Polarography

2020 ◽  
Vol 2020 ◽  
pp. 1-9
Author(s):  
M. Azimi ◽  
M. Ahmadi Golsefidi ◽  
A. Varasteh Moradi ◽  
M. Ebadii ◽  
R. Zafar Mehrabian
Keyword(s):  
Molecularly Imprinted Polymer ◽  
Limit Of Detection ◽  
Diabetes Type 2 ◽  
Differential Pulse ◽  
Template Molecule ◽  
Imprinted Polymer ◽  
Limit Of Quantification ◽  
Molecularly Imprinted ◽  
Galega Officinalis ◽  
Relative Standard

Galega officinalis products have been used for the control of diabetes (type 2) across the world. Experimental and clinical evaluations of galegine substance produced by a medicinal plant (Galega officinalis) provided the pharmacological and chemical basis for metformin discovery which was confirmed for diabetes therapy. In this paper, the molecularly imprinted polymer (MIP) was synthesized for galegine, using galegine as a template molecule, methacrylic acid (MAA) as a functional monomer, ethylene glycol dimethacrylate (EGDMA) as a cross-linker, azobisisobutyronitrile (AIBN) as a reaction initiator, and acetonitrile as a solvent. The assisted functional groups, morphology, topographic image of surface, and crystalline structure of synthesized MIP were characterized by FT-IR spectroscopy, field emission scanning electron microscopy (FE-SEM), atomic force microscopy (AFM) images, and XRD diffraction pattern techniques, respectively. Also, the performance of the mentioned electrode was quantified and qualified by the differential pulse voltammetry technique (DPV). The galegine amount was determined with the polarographic technique. In this research, the galegine extraction conditions were optimized and graphene nanoparticles were used to increase the adsorption. In addition, different parameters affecting extraction were investigated such as MIP adsorbent amount, pH of solution, effect of the surfactant, and ionic compound to achieve high recovery percent. The recovery percent, limit of detection (LOD), limit of quantification (LOQ), and relative standard deviation (RSD %) were 4.101 μg·mL−1, 12.427 μg·mL−1, and 1.199% (n = 3), respectively. The results show that the prepared MIP can be used as an effective and inexpensive adsorbent for preconcentration and galegine extraction from a natural sample. It is noteworthy that this developed method was used successfully to determine galegine extracted from Galega officinalis L.

scholarly journals Highly sensitive detection of estradiol by a SERS sensor based on TiO2 covered with gold nanoparticles

2020 ◽  
Vol 11 ◽  
pp. 1026-1035 ◽  
Author(s):  
Andrea Brognara ◽  
Ili F Mohamad Ali Nasri ◽  
Beatrice R Bricchi ◽  
Andrea Li Bassi ◽  
Caroline Gauchotte-Lindsay ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Dynamic Range ◽  
Limit Of Detection ◽  
Gold Deposition ◽  
Annealing Conditions ◽  
Highly Sensitive ◽  
Surface Enhanced Raman ◽  
17Β Estradiol ◽  
Enhanced Raman Scattering ◽  
Highly Sensitive Detection

We propose the use of gold nanoparticles grown on the surface of nanoporous TiO2 films as surface-enhanced Raman scattering (SERS) sensors for the detection of 17β-estradiol. Gold deposition on top of a TiO2 surface leads to the formation of nanoparticles the plasmonic properties of which fulfil the requirements of a SERS sensor. The morphological and optical properties of the surface were investigated. Specifically, we demonstrate that the TiO2 background pressure during pulsed laser deposition and the annealing conditions offer control over the formation of Au nanoparticles with different sizes, shapes and distributions, yielding a versatile sensor. We have exploited the surface for the detection of 17β-estradiol, an emerging contaminant in environmental waters. We have found a limit of detection of 1 nM with a sensitivity allowing for a dynamic range of five orders of magnitude (up to 100 µM).

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