Genetic and morphological variability in cestodes of the genus Proteocephalus: geographical variation in Proteocephalus percae populations

1999 ◽  
Vol 77 (9) ◽  
pp. 1450-1458 ◽  
Author(s):  
Vladimíra Hanzelová ◽  
Viliam Šnábel ◽  
Ivica Král'ová ◽  
Tomáš Scholz ◽  
Stefano D'Amelio
Keyword(s):  
Random Amplified Polymorphic Dna ◽  
Morphological Variability ◽  
Population Sample ◽  
Natural Populations ◽  
Water Reservoir ◽  
Perca Fluviatilis ◽  
Morphological Characters ◽  
Rapd Profile ◽  
Perch Perca Fluviatilis ◽  
Apical Sucker

Genetic and morphological variability was studied in the tapeworm Proteocephalus percae (Müller, 1780) (Cestoda: Proteocephalidae), a parasite of perch (Perca fluviatilis L.). Geographic variation in isoenzyme patterns, random amplified polymorphic DNA (RAPD) profiles, and morphological characters were found within six natural populations of P. percae from Slovakia and Switzerland. All approaches used showed that similarity was substantially higher among Swiss populations than among Slovak populations. Proteocephalus percae specimens from the Dobšiná water reservoir (Slovakia) differed significantly from all other population sample sets in that they exclusively expressed the genotype Acp92/Acp92 and possessed a specific RAPD profile after amplification with the primer p78 and distinct measurements of the scolex (scolex width and apical sucker). The unique position of the P. percae population from Dobšiná could be related to the contamination of this locality with heavy metals (cadmium), through the possible impact of these metals on the alterations observed. The taxonomic relevance and stability of the morphological characters related to the scolex in the Proteocephalus tapeworm group is confirmed.

scholarly journals The Contribution of Perch (Perca fluviatilis L. 1758) to Maintaining the Diphyllobothriasis Focus in the Krasnoyarsk Reservoir and the Yenisei River

2020 ◽  
pp. 297-309
Author(s):  
Julia K. Chugunova ◽  
Tatyana Yu. Ronzhina ◽  
Alexey A. Syromyatnikov
Keyword(s):  
Water Reservoir ◽  
Perca Fluviatilis ◽  
Fish Muscle ◽  
Middle Part ◽  
Yenisei River ◽  
Leading Role ◽  
The People ◽  
Perch Perca Fluviatilis ◽  
The Yenisei River ◽  
Diphyllobothrium Latum

The study investigates infection rates of perch by plerocercoids of the Dibothriocephalus latus (Linnaeus, 1758) Lühe, 1899 (syn. Diphyllobothrium latum) broad tapeworm in the creeks of the Krasnoyarsk Water Reservoir and in the Yenisei River (within the limits of the city of Krasnoyarsk). Plerocercoids were found in the perch in all study areas. The rates of invasion of the fish in the Yenisei River and in the creeks of the lower part of the Reservoir – Biryusa and Shumikha – were low (9.8, 6.9, and 13.5%, respectively); the infection of perch was higher in the middle part of the Reservoir, in the Sisim Creek (21.4%). The fish purchased at an unauthorized market was the most infected – 68.5%. The sample included large fish, weighing from 70 to 970 g. A positive correlation was noted between invasion rate and fish age (r=0.7). The plerocercoids found in the fish were viable. They were mainly localized in the fish muscle. Diphyllobothriosis is a commonly occurring helminthiasis in the riverine communities transformed because of the damming of rivers. In the Krasnoyarsk Water Reservoir, the broad tapeworms are usually transmitted along the plankton – perch – pike chain. The data obtained in this study show that small perch, consuming infected plankton, plays a leading role in maintaining the abundance of D. latus in the water reservoir. However, the main contribution to the epidemiological situation in the region is made by the large perch, which is heavily infected with broad tapeworm plerocercoids because of its cannibalistic feeding behavior. Both recreational fishermen and the people purchasing fish at unauthorized markets prefer large perch. Commercial and recreational fishing, settlements on the shores of the Reservoir, and the increasing number of tourists have contributed to the invasion of pathogenic organisms in the Reservoir. The perch caught in the Krasnoyarsk Water Reservoir or in the Yenisei River or purchased at unauthorized markets, which have not been inspected for disease, could be a source of diphyllobothriosis infection for people

EVALUATION OF PTYCHOBOTHRIDEAN CESTODES USING RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) MARKERS FROM FRESH WATER FISHES IN GODAVARI BASIN (M.S.) INDIA

2017 ◽  
Vol 23 (2) ◽  
Author(s):  
SUNITA BORDE ◽  
ASAWARI FARTADE ◽  
AMOL THOSAR ◽  
RAHUL KHAWAL
Keyword(s):  
Fresh Water ◽  
Rapd Markers ◽  
Random Amplified Polymorphic Dna ◽  
Band Pattern ◽  
Genomic Variation ◽  
Polymorphic Band ◽  
Rapd Profile ◽  
Band Size ◽  
Pcr Product ◽  
The Common

Ptychobothridean genera like Senga and Circumoncobothrium are the common parasites of fresh water fishes. The genotypic study of these parasites was taken by RAPD. The RAPD profile of these two parasites were not similar to each other as depicted by the band pattern in picture. These results suggest the presence of inter-specific polymorphism among cestode parasites of two different genera for RAPD analysis. The present study demonstrated that genetic differentiation of cestode parasites could be accomplished on the basis of genomic variation with polymorphic band pattern using RAPD. All the detected bands (PCR product) were polymorphic and band size ranged from 500-5000 bp in length. The RAPD of profiles using GBO-31, GBO-32, GBO-33, GBO-34, GBO-35 and GBO-36. Primers were able to characterize inter-specific polymorphism among the two genus ( Senga and Circumoncobothrium ). Genetic analysis suggests that Senga and Circumoncobothrium show genetic diversity with respect to RAPD patterns using all the six primers used for the present study. The genetic distance between the analyzed genuses ranged from 0.14 to 0.80. The differentiation of the two parasites on the basis of genetic markers could greatly facilitate study on the biology of these parasites.

Consumption of plant material by perch (Perca fluviatilis)

2016 ◽  
Vol 65 (2) ◽  
pp. 95-97 ◽  
Author(s):  
Tomáš Zapletal ◽  
Zdeněk Adámek ◽  
Pavel Jurajda ◽  
Kevin Roche ◽  
Lucie Všetičková ◽  
...  
Keyword(s):  
Plant Material ◽  
Perca Fluviatilis ◽  
Perch Perca Fluviatilis

Short-term storage of perch (Perca fluviatilis L.) milt under cooling conditions

2013 ◽  
Vol 13 ◽  
pp. 39 ◽  
Author(s):  
Beata Sarosiek ◽  
Beata I. Cejko ◽  
Dariusz Kucharczyk ◽  
Daniel Żarski ◽  
Sylwia Judycka ◽  
...  
Keyword(s):  
Perca Fluviatilis ◽  
Short Term ◽  
Cooling Conditions ◽  
Perch Perca Fluviatilis ◽  
Short Term Storage ◽  
Term Storage
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