Prolonged storage of spermatozoa in the genital tract of female Mexican big-eared bats (Corynorhinus mexicanus): the role of lipid peroxidation

1999 ◽  
Vol 77 (1) ◽  
pp. 7-12 ◽  
Author(s):  
Miguel Angel León-Galvan ◽  
Teresa Fonseca ◽  
Ricardo López-Wilchis ◽  
Adolfo Rosado
Keyword(s):  
Lipid Peroxidation ◽  
Genital Tract ◽  
Female Genital Tract ◽  
Reproductive Activity ◽  
Sperm Storage ◽  
Prolonged Storage ◽  
Corpora Lutea ◽  
Dependent Inhibition ◽  
Significant Concentration

The results of this work support the existence of sperm storage in Corynorhinus mexicanus, a vespertilionid bat endemic to Mexico, and evidence is presented that modulation of peroxidative toxicity plays a role in the mechanism of prolonged sperm storage in Chiroptera. Spermatozoa were obtained from C. mexicanus by retrograde perfusion of the cauda epididymis, and from genital washings from previously inseminated females captured during reproductive activity. Ejaculated pig spermatozoa were simultaneously studied as controls. Lipid peroxidation was determined in both the presence and absence of genital secretions obtained from previously inseminated female C. mexicanus by measuring malondialdehyde generated during aerobic incubation of spermatozoa suspensions. The number of spermatozoa recovered from the cauda epididymis decreased steadily from November through January. Corpora lutea were observed in January. None of the female bats captured between October and January were pregnant, but some females captured in mid-February were already pregnant. Spermatozoa of C. mexicanus showed substantial lipid peroxidation activity (0.64 ± 0.11 nmol malondialdehyde was produced by 108 spermatozoa per 22 h), about half of that observed in ejaculated pig spermatozoa. Incubation of pig and C. mexicanus spermatozoa in the presence of bat female genital tract washings induced highly significant concentration-dependent inhibition of lipid peroxidation.

Prolonged storage of spermatozoa in the genital tract of female Mexican big-eared bats (Corynorhinus mexicanus): the role of lipid peroxidation

1999 ◽  
Vol 77 (1) ◽  
pp. 7-12 ◽  
Author(s):  
Miguel Angel León-Galvan ◽  
Teresa Fonseca ◽  
Ricardo López-Wilchis ◽  
Adolfo Rosado
Keyword(s):  
Lipid Peroxidation ◽  
Genital Tract ◽  
Prolonged Storage

Genital mycoplasmas, including Mycoplasma genitalium, as sexually transmitted agents

2002 ◽  
Vol 13 (2) ◽  
pp. 79-85 ◽  
Author(s):  
Anneli Uusküla ◽  
Peter K Kohl
Keyword(s):  
Genital Tract ◽  
Ureaplasma Urealyticum ◽  
Female Genital Tract ◽  
Mycoplasma Species ◽  
Case Control Studies ◽  
Pathogenic Potential ◽  
Intracellular Location ◽  
Main Site ◽  
Sexually Transmitted

Mycoplasmas are the smallest free-living organisms, widespread in nature. Several mycoplasma species have been isolated from humans. For 6 of them: Mycoplasma hominis, Ureaplasma urealyticum, M. primatum, M. genitalium, M. spermatophilum and M. penetrans, the genital tract is the main site of colonization. This review is concentrated on the role of mycoplasmas as sexually transmitted agents, with the emphasis to M. genitalium infections. M. hominis and U. urealyticum are isolated from the genital tract of healthy men and women with considerable frequency. The biological features (attachment properties, possible intracellular location) and experimental inoculation studies of M. genitalium indicate that this mycoplasma has pathogenic potential. Data from case-control studies, looking at men with non-gonococcal urethritis and women with cervicitis, have revealed that M. genitalium behave similarly to Chlamydia trachomatis and have revealed that carriage of M. genitalium and C. trachomatis is usually independent of one another. M. genitalium could be considered as a potential cause of sexually transmitted urethritis in men, including men with persistent or recurrent urethritis. More studies are expected to ascertain the role of M. genitalium in the female genital tract. Evidence-based data are needed to decide whether current non-gonococcal infection treatment principles are applicable or not for M. genitalium infections.

Evidence for cGAS-STING signaling in the female genital tract resistance to Chlamydia trachomatis infection

2022 ◽  
Author(s):  
Xin Su ◽  
Hong Xu ◽  
Maegan French ◽  
Yujie Zhao ◽  
Lingli Tang ◽  
...  
Keyword(s):  
Innate Immunity ◽  
Chlamydia Trachomatis ◽  
Signaling Pathway ◽  
Genital Tract ◽  
Essential Role ◽  
Cultured Cells ◽  
Female Genital Tract ◽  
Lower Genital Tract ◽  
Female Genital

Sexually transmitted Chlamydia trachomatis can ascend to the upper genital tract due to its resistance to innate immunity in the lower genital tract. C. trachomatis can activate cGAS-STING signaling pathway in cultured cells via either cGAS or STING. The current study was designed to evaluate the role of the cGAS-STING pathway in innate immunity against C. trachomatis in the mouse genital tract. Following intravaginal inoculation, C. trachomatis significantly declined by day 5 following a peak infection on day 3 while the mouse-adapted C. muridarum continued to rise for >1 week, indicating that C. trachomatis is susceptible to the innate immunity in the female mouse genital tract. This conclusion was supported by the observation of a similar shedding course in mice deficient in adaptive immunity. Thus, C. trachomatis can be used to evaluate innate immunity in the female genital tract. It was found that mice deficient in either cGAS or STING significantly increased the yields of live C. trachomatis on day 5, indicating an essential role of the cGAS-STING signaling pathway in innate immunity of the mouse genital tract. Comparison of live C. trachomatis recovered from different genital tissues revealed that the cGAS-STING-dependent immunity against C. trachomatis was restricted to the mouse lower genital tract regardless of whether C. trachomatis was inoculated intravaginally or transcervically. Thus, we have demonstrated an essential role of the cGAS-STING signaling pathway in innate immunity against chlamydial infection, laying a foundation for further illuminating the mechanisms of the innate immunity in the female lower genital tract.

Surgical Prevention in Ovarian Cancer

2021 ◽  
pp. 194-206
Author(s):  
Alexios Papanikolaou ◽  
Anastasios Liberis ◽  
Anastasia Vatopoulou
Keyword(s):  
Ovarian Cancer ◽  
Primary Prevention ◽  
Early Detection ◽  
Early Diagnosis ◽  
Genital Tract ◽  
Female Genital Tract ◽  
Current Evidence ◽  
Advanced Stage ◽  
Stage Disease

Ovarian cancer is the second most common malignant disease of the female genital tract, but the first in mortality because it is usually diagnosed at an advanced stage. Options for early detection, diagnosis, and treatment are limited. Prevention of ovarian cancer relates to primary prevention by avoiding factors that are epidemiologically associated with an increased incidence of ovarian cancer and the adoption of protective habits. These include interventions to exclude the fallopian tubes and ovaries. Secondary prevention is related to early diagnosis. The chapter aims to summarize current evidence on prevention of ovarian cancer as well as role of surgery to prevent advanced-stage disease.

scholarly journals Investigating the role of immunotherapy in advanced/recurrent female genital tract melanoma: a preliminary experience

2019 ◽  
Vol 30 (6) ◽  
Author(s):  
Alice Indini ◽  
Lorenza Di Guardo ◽  
Carolina Cimminiello ◽  
Domenica Lorusso ◽  
Francesco Raspagliesi ◽  
...  
Keyword(s):  
Genital Tract ◽  
Female Genital Tract ◽  
Female Genital

Role of Semen in Altering the Balance Between Inflammation and Tolerance in the Female Genital Tract: Does it Contribute to HIV Risk?

2014 ◽  
Vol 27 (5) ◽  
pp. 200-206 ◽  
Author(s):  
Cosnet L. Rametse ◽  
Abraham J. Olivier ◽  
Lindi Masson ◽  
Shaun Barnabas ◽  
Lyle R. McKinnon ◽  
...  
Keyword(s):  
Hiv Risk ◽  
Genital Tract ◽  
Female Genital Tract ◽  
Female Genital
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