Antibacterial activity of the seminal plasma of Paracentrotus lividus

1994 ◽  
Vol 72 (7) ◽  
pp. 1211-1216 ◽  
Author(s):  
Loredana Stabili ◽  
Calogero Canicattì
Keyword(s):  
Antibacterial Activity ◽  
Seminal Plasma ◽  
Bacterial Growth ◽  
Bacterial Cell ◽  
Inhibitory Action ◽  
Paracentrotus Lividus ◽  
Cell Number ◽  
Morphological Examination ◽  
Bacterial Cell Number ◽  
Antibacterial Factor

Seminal plasma from Paracentrotus lividus exerted an inhibitory action on the growth of bacterial colonies. The antibacterial reaction took 30 min to reach full expression and depended on both the dose of seminal plasma and the bacterial cell number. Heating at 56 °C for 60 min did not lower the antibacterial power of the seminal plasma. Morphological examination of bacteria treated with seminal plasma revealed a conspicuous alteration of their surface and suggested a lytic mode of action for the antibacterial factor(s). Lysozyme could be involved in this process. In fact, inhibition of bacterial growth strongly decreased when this hydrolase was inactivated by heating at a basic pH.

scholarly journals Consideration of Influential Factors on Bioleaching of Gold Ore Using Iodide-Oxidizing Bacteria

Minerals ◽  
2019 ◽  
Vol 9 (5) ◽  
pp. 274 ◽  
Author(s):  
San Yee Khaing ◽  
Yuichi Sugai ◽  
Kyuro Sasaki ◽  
Myo Min Tun
Keyword(s):  
Potassium Iodide ◽  
Bacterial Cell ◽  
Cell Number ◽  
Culture Solution ◽  
Gold Dissolution ◽  
Marine Broth ◽  
Gold Leaching ◽  
Temperature Range ◽  
Gold Ore ◽  
Bacterial Cell Number

Iodide-oxidizing bacteria (IOB) oxidize iodide into iodine and triiodide which can be utilized for gold dissolution. IOB can be therefore useful for gold leaching. This study examined the impact of incubation conditions such as concentration of the nutrient and iodide, initial bacterial cell number, incubation temperature, and shaking condition on the performance of the gold dissolution through the experiments incubating IOB in the culture medium containing the marine broth, potassium iodide and gold ore. The minimum necessary concentration of marine broth and potassium iodide for the complete gold dissolution were determined to be 18.7 g/L and 10.9 g/L respectively. The initial bacterial cell number had no effect on gold dissolution when it was 1 × 104 cells/mL or higher. Gold leaching with IOB should be operated under a temperature range of 30–35 °C, which was the optimal temperature range for IOB. The bacterial growth rate under shaking conditions was three times faster than that under static conditions. Shaking incubation effectively shortened the contact time compared to the static incubation. According to the pH and redox potential of the culture solution, the stable gold complex in the culture solution of this study could be designated as gold (I) diiodide.

scholarly journals Efficacy Bacteriophage in Removal Pseudomonas aeruginosa from Infectious Surfaces

2021 ◽  
Author(s):  
Golnar Rahimzadeh ◽  
Mohammad Sadegh Rezai
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Nosocomial Infection ◽  
Nosocomial Infections ◽  
Bacterial Cell ◽  
Cell Number ◽  
Biocontrol Agents ◽  
Hospital Environment ◽  
Bacterial Cells ◽  
Bacterial Cell Number

Nosocomial infections can be transmitted by contaminated hospital surfaces with resistant pathogens. conventional sanitations are not efficiently contributing to removing resistant pathogens. Bacteriophages suggest as decontaminating agents, safe, their selective ability to kill specific bacteria. This work aimed to assess the efficiency of a phage in removing Pseudomonas aeruginosa from different hard surfaces. The decontamination ability of phages w was tested in vitro against Pseudomonas aeruginosa strain. Cystoviridae Phages with titer (2 × 1012 PFU/mL) can efficiently reduce viable bacterial cells on contaminated surfaces. The treated surfaces with alcohol 70% and phage showed an evident drop of bacterial cell number from 1 h to 24 h. These results suggest that bacteriophages are biocontrol agents removing nosocomial infection pathogens transmitted by contaminated surfaces in the hospital environment.

scholarly journals Estimation of lactic acid bacterial cell number by DNA quantification

2018 ◽  
Vol 12 (2) ◽  
pp. 88-91 ◽  
Author(s):  
Masaki Ishii ◽  
Yasuhiko Matsumoto ◽  
Kazuhisa Sekimizu
Keyword(s):  
Lactic Acid ◽  
Bacterial Cell ◽  
Cell Number ◽  
Dna Quantification ◽  
Bacterial Cell Number

Development of a bioluminescent ATP assay to quantify mammalian and bacterial cell number from a mixed population

Biomaterials ◽  
2003 ◽  
Vol 24 (1) ◽  
pp. 27-34 ◽  
Author(s):  
Sarah J. Dexter ◽  
Miguel Cámara ◽  
Martyn Davies ◽  
Kevin M. Shakesheff
Keyword(s):  
Bacterial Cell ◽  
Cell Number ◽  
Mixed Population ◽  
Atp Assay ◽  
Bacterial Cell Number

Evaluation of Polyphenolic Profile and Antibacterial Activity of Pomegranate Juice in Combination with Rifampin (R) against MDR-TB Clinical Isolates

2019 ◽  
Vol 20 (4) ◽  
pp. 317-326 ◽  
Author(s):  
Manaf AlMatar ◽  
Işıl Var ◽  
Begüm Kayar ◽  
Emel Eker ◽  
Ebru Kafkas ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Phenolic Compounds ◽  
Synergistic Effect ◽  
Bacterial Growth ◽  
Total Flavonoid ◽  
Clinical Isolates ◽  
Pomegranate Juice ◽  
Total Phenolic ◽  
Total Anthocyanin ◽  
Mdr Tb

Background: The global rise of multi-drug resistant M. tuberculosis demands unconventional treatment to enhance the efficiency of current drugs. Punica granatum, which is known as pomegranate, is considered as a member of the Punicaceae family. Pomegranate, which is broadly documented for its activity against a wide spectrum of bacterial pathogens, deserves further scrutiny in this respect. Methods: Within this scope, this study investigated the effect of fresh pomegranate juice (FPJ) on the antibacterial activity of anti-tuberculosis drugs (Rifampin (R) and Isoniazid (INH)) against MDR-TB clinical isolates. The drug resistance profiles in M. tuberculosis clinical isolates were determined by susceptibility test using BACTEC MGIT 960 system. Four concentrations of fresh pomegranate juice (FPJ) (5%, 10%, 15%, and 20%) were evaluated in combination with R and INH at a dose range of (1.0 µg/ml) and (0.1 µg/ml), respectively against the MDR-TB isolates by the BACTEC MGIT 960 system. Moreover, this study scrutinized individual phenolic compounds of FPJ by using highperformance liquid chromatography (HPLC). The total polyphenols (TP), total flavonoid (TF), total anthocyanins content (TAC), and the antioxidant capacity were also assessed in FPJ. Results: Synergistic effects were observed between R and INH with FPJ against all tested strains. However, combination therapy of rifampin was more effective than isoniazid one. Therefore, the combination of R and FPJ has been used against (27) MDR-TB clinical isolates. 5% of FPJ plus R (1.0 µg/ml) were found to suppress the growth of one isolates for first group (INH and R resistant). However, 5% of FPJ demonstrated no synergistic impact with R for second (SM, R and INH resistant) and third group (INH, EMB, R and SM resistant). Moreover, 10% of FPJ and R (1.0 μg/ml) inhibited the bacterial growth of three isolates of first group and two isolates and one isolate for second and third group, respectively. Remarkably, 15% of FPJ plus R (1.0 µg/ml) appeared to inhibit the growth of MDR-TB isolates for all tested groups indicating a strong synergistic effect. Regarding H37RV, the complete inhibition of the bacterial growth was found to occur at 15% and 20% concentrations of FPJ only. Minimum inhibitory concentration (MIC) of FPJ ranged from (4% to13%) for first group and from (10% to15%) for second and third group. Thus, FPJ at 15% inhibited 100% of bacteria for all tested isolates (MIC100% =15%). Phenolic compounds identified in FPJ were gallic acid, benzoic acid, syringic, folic acid, pelargonidin, naringin+ellagic acid, naringenin, chlorogenic acid, caffeic acid, catechin, myricetin, kaempferol, quercetin, cyanidin-3-glycoside, p-cummaric acid, ferulic acid, and rutin. Total phenolic (TP), total flavonoid (TF), and total anthocyanin (TA) content were 841.5 mg/L, 638.73 mg RE/L, and 47.43 mg/L, accordingly. Conclusion: Overall, FPJ displayed synergistic effect with R against MDR-TB clinical isolates due to its high content of polyphenol and antioxidant capability.

scholarly journals Effect of tannic and gallic acids alone or in combination with carbenicillin or tetracycline on Chromobacterium violaceum CV026 growth, motility, and biofilm formation

2015 ◽  
Vol 61 (7) ◽  
pp. 487-494 ◽  
Author(s):  
Devendra H. Dusane ◽  
Che O’May ◽  
Nathalie Tufenkji
Keyword(s):  
Antibacterial Activity ◽  
Gallic Acid ◽  
Tannic Acid ◽  
Bacterial Growth ◽  
Biofilm Formation ◽  
Opportunistic Pathogen ◽  
Chromobacterium Violaceum ◽  
Minimum Inhibitory Concentrations

Chromobacterium violaceum is an opportunistic pathogen that causes infections that are difficult to treat. The goal of this research was to evaluate the effect of selected tannins (tannic acid (TA) and gallic acid (GA)) on bacterial growth, motility, antibiotic (carbenicillin, tetracycline) susceptibility, and biofilm formation. Both tannins, particularly TA, impaired bacterial growth levels and swimming motilities at sub-minimum inhibitory concentrations (sub-MICs). In combination with tannins, antibiotics showed increased MICs, suggesting that tannins interfered with antibacterial activity. Sub-MICs of tetracycline or TA alone enhanced biofilm formation of C. violaceum; however, in combination, these compounds inhibited biofilm formation. In contrast, carbenicillin at sub-MICs was effective in inhibiting C. violaceum biofilm formation; however, in combination with lower concentrations of TA or GA, biofilms were enhanced. These results provide insights into the effects of tannins on C. violaceum growth and their varying interaction with antibiotics used to target C. violaceum infections.

scholarly journals In vitro Antibacterial Activity of Organic Extracts of Aloe barbadensis against Multi-drug resistant Pseudomonas aeruginosa Isolated from Wound Specimens

2018 ◽  
Vol 5 ◽  
pp. 69-76
Author(s):  
Mamata Adhikari ◽  
Anil Kumar Sah ◽  
Dev Raj Joshi
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Inhibitory Action ◽  
Therapeutic Option ◽  
Diffusion Method ◽  
Drug Resistant ◽  
Aloe Barbadensis ◽  
Organic Extracts ◽  
In Vitro Antibacterial Activity

Objectives: In order to investigate alternate therapeutic option, this study was carried out to assess the in vitro antibacterial activity of gel extract of Aloe barbadensis against multiple antibiotic resistant Pseudomonas aeruginosa isolated from wound specimens. Methods: A total of 180 different wound specimens collected in a hospital, were subjected to isolate and identify P. aeruginosa by cultural methods. Antibiotic susceptibility testing was done by Kirby- Bauer disc diffusion method to screen multidrug resistant isolates. A. barbadensis extracts were prepared using aqueous and organic solvents and their in vitro inhibitory action was evaluated by agar well diffusion methods. Results: Out of total, 38 (21.1%) of the wound specimens showed the occurrence of P. aeruginosa, among which 15 (39%) isolates were multi-drug resistant. Organic extracts of various concentrations (0.2 - 0.8 v/v %) inhibited 66.7% of MDR and all non-MDR (n = 23) P. aeruginosa with zone of inhibition ranging from 9.5 ±1.0 to 21.3 ± 2.2 mm but not by aqueous extract. A positive Pearson’s correlation (r=0.983) was found between antibacterial effect and concentrations of the extracts. The antibacterial activity of organic extracts was statistically associated with antibiotic resistance profile of the organism (p<0.05). Conclusion: Organic extracts of A. barbadensis revealed variable in vitro inhibitory action against both MDR and non-MDR P. aeruginosa isolated from wound specimens. Although further confirmation is needed, aloe gel extract may be applied as an alternate treatment option.

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