Adrenergic stimulation of glycolysis without change in glycolytic enzyme binding in rainbow trout (Oncorhynchus mykiss) erythrocytes

1994 ◽  
Vol 72 (5) ◽  
pp. 950-953 ◽  
Author(s):  
Dawn H. Sephton ◽  
William R. Driedzic
Keyword(s):  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Whole Blood ◽  
Glycolytic Enzyme ◽  
Adrenergic Stimulation ◽  
Enzyme Binding ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Cell Membrane Binding ◽  
Stimulation Of

Whole blood from rainbow trout (Oncorhynchus mykiss) was incubated in vitro with pharmacological levels of isoproterenol. The adrenergic stimulation of glycolysis in erythrocytes (RBCs) was assessed by monitoring the rate of 14C incorporation from [6-14C]-glucose into the acid-soluble RBC fraction. During a 3-h in vitro incubation, incorporation of label into the acid-soluble RBC fraction of isoproterenol-treated whole blood (0.25 ± 0.04 μmol glucose∙mL−1 RBC∙h−1) was higher than into untreated blood (0.08 ± 0.01 μmol glucose∙mL−1 RBC∙h−1). The percentage of cell membrane binding for phosphofructokinase, aldolase, and glyceraldehyde-3-phosphate dehydrogenase ranged from 17 to 35% and was not altered by adrenergic stimulation. Adrenergic stimulation activates glycolysis in rainbow trout RBCs but not through the modulation of enzyme binding.

Estrogenic activity of multicyclic aromatic hydrocarbons in rainbow trout (Oncorhynchus mykiss) in vitro assays

2019 ◽  
Vol 207 ◽  
pp. 43-51 ◽  
Author(s):  
Richard C. Kolanczyk ◽  
Jeffrey S. Denny ◽  
Barbara R. Sheedy ◽  
Patricia K. Schmieder ◽  
Mark A. Tapper
Keyword(s):  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Aromatic Hydrocarbons ◽  
Estrogenic Activity ◽  
In Vitro Assays ◽  
Rainbow Trout Oncorhynchus Mykiss

scholarly journals Desensitization of Adrenaline-Induced Red Blood Cell H+ Extrusion in Vitro After Chronic Exposure of Rainbow Trout to Moderate Environmental Hypoxia

1991 ◽  
Vol 156 (1) ◽  
pp. 233-248 ◽  
Author(s):  
S. THOMAS ◽  
R. KINKEAD ◽  
P. J. WALSH ◽  
C. M. WOOD ◽  
S. F. PERRY
Keyword(s):  
Rainbow Trout ◽  
Blood Cell ◽  
Red Blood Cell ◽  
Whole Blood ◽  
Inverse Correlation ◽  
Adrenergic Stimulation ◽  
Plasma Adrenaline ◽  
Arterial Blood Gas ◽  
Arterial Blood

The sensitivity of red blood cell Na+/H+ exchange to exogenous adrenaline was assessed in vitro using blood withdrawn from catheterized rainbow trout (Oncorhynchus mykiss) maintained under normoxic conditions [water PO2, (PwO2)=20.66 kPa] or after exposure to moderate hypoxia (PwO2=6.67-9.33 kPa) for 48 h, which chronically elevated plasma adrenaline, but not noradrenaline, levels. Peak changes in whole-blood extracellular pH over a 30 min period after adding 50–1000 nmoll−1 adrenaline were employed as an index of sensitivity; the blood was pre-equilibrated to simulate arterial blood gas tensions in severely hypoxic fish (PaO2=2.0 kPa, PaCO2=0.31 kPa). Blood pooled from normoxic fish displayed a dose-dependent reduction in whole-blood pH after addition of adrenaline. Blood pooled from three separate groups of hypoxic fish, however, displayed diminished sensitivity to adrenaline, ranging from complete desensitization to a 60%reduction of the response. Subsequent experiments performed on blood from individual (i.e. not pooled) normoxic or hypoxic fish demonstrated an inverse correlation between the intensity of H+ extrusion (induced by exogenous adrenaline addition) and endogenous plasma adrenaline levels at the time of blood withdrawal. However, acute increases in plasma adrenaline levels in vitro did not affect the responsiveness of the red blood cell to subsequent adrenergic stimulation. The intensity of H+ extrusion was inversely related to the PaO2in vivo between 2.67 and 10.66 kPa, and directly related to the logarithm of the endogenous plasma adrenaline level. The results suggest that desensitization of Na+/H+ exchange in chronically hypoxic fish is related to persistent elevation of levels of this catecholamine. This desensitization can be reversed in vitro as a function of time, but only when blood is maintained under sufficiently aerobic conditions.

Insulin and insulin-like growth factor I signaling pathways in rainbow trout (Oncorhynchus mykiss) during adipogenesis and their implication in glucose uptake

2010 ◽  
Vol 299 (1) ◽  
pp. R33-R41 ◽  
Author(s):  
L. Bouraoui ◽  
E. Capilla ◽  
J. Gutiérrez ◽  
I. Navarro
Keyword(s):  
Rainbow Trout ◽  
Growth Factor ◽  
Oncorhynchus Mykiss ◽  
Glucose Uptake ◽  
Signaling Pathways ◽  
Mapk Phosphorylation ◽  
Factor I ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Igf I ◽  
Stimulation Of

Primary cultures of rainbow trout ( Oncorhynchus mykiss ) adipocytes were used to examine the main signaling pathways of insulin and insulin-like growth factor I (IGF-I) during adipogenesis. We first determined the presence of IGF-I receptors (IGF-IR) and insulin receptors (IR) in trout preadipocytes ( day 5) and adipocytes ( day 14). IGF-IRs were more abundant and appeared to be in higher levels in differentiated cells than in preadipocytes, whereas IRs were detected in lower but constant levels throughout the culture. The cells were immunoreactive against ERK1/2 MAPK, and AKT/PI3K, components of the two main signal transduction pathways for insulin and IGF-I receptors. Stimulation of MAPK phosphorylation by IGF-I was higher in preadipocytes than in adipocytes, while no effects were observed in MAPK phosphorylation after incubation of cells with insulin. AKT phosphorylation increased in the presence of both insulin and IGF-I, with higher levels of stimulation in adipocytes than in preadipocytes. Activation of both pathways was blocked by the use of specific inhibitors of MAPK (PD98059) and AKT (wortmannin). We describe here, for the first time, the effects of IGF-I and insulin on 2-deoxyglucose uptake in primary culture of trout adipocytes. IGF-I was more potent in stimulating glucose uptake than insulin, and PD98059 and wortmannin inhibited the stimulation of glucose uptake by this growth factor, suggesting that IGF-I plays an important metabolic role in trout adipocytes. Our results suggest that differential activation of the MAPK and AKT pathways are involved in the IGF-I- and insulin-induced effects of trout adipocytes during the various stages of adipogenesis.

scholarly journals Gill amoebae from freshwater rainbow trout ( Oncorhynchus mykiss ): In vitro evaluation of antiparasitic compounds against Vannella sp.

2020 ◽  
Vol 43 (6) ◽  
pp. 665-672
Author(s):  
Hannah Malene Jensen ◽  
Asma Mohammad Karami ◽  
Heidi Mathiessen ◽  
Azmi Al‐Jubury ◽  
Per Walter Kania ◽  
...  
Keyword(s):  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
In Vitro Evaluation ◽  
Rainbow Trout Oncorhynchus Mykiss
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